Labshake search
Citations for Qiagen :
751 - 800 of 4054 citations for 6 methyl 4 oxo N phenyl 2 3 dihydro 1 4 oxathiine 5 carboxamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The coverslips were then incubated for ~ 10 min with 4 μg/ml of Penta•His biotin conjugate antibody (34440, Qiagen, UK) in reaction buffer [40 mM HEPES buffer (pH 7.3 ...
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bioRxiv - Cancer Biology 2022Quote: ... Variants in variant call format files were evaluated for pathogenicity using Ingenuity Variant Analysis (IVA) version 4 (Qiagen Inc, Alameda, CA) and American College of Medical Genetics and Genomics (ACMGG ...
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bioRxiv - Microbiology 2022Quote: ... microbial genomic DNA (gDNA) was extracted from possum excreta samples using the DNeasy PowerSoil HTP 96 Kit (Qiagen Cat# 12955-4) following the manufacturer’s protocols just prior to the addition of solution C4 ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were centrifuged at 14,000 x g for 20 min (4°C) to pellet insoluble cell debris and supernatants were transferred to violet QIAshredder mini spin columns (Qiagen, Australia) and centrifuged again for one minute ...
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bioRxiv - Genomics 2022Quote: ... 4°C) and stored at −80°C before metagenomic extraction using the DNeasy PowerSoil kit following manufacturer specifications (QIAGEN, Hilden, Germany). All sampling and sample processing was conducted following cold chain principles ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from MCF10AER/vSrc cells (Control and treated with 10 nM D-Alanine for 4 h or 24 h) using RNeasy Kit (Qiagen, #74104) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Cells were harvested after 72 hours siRNA knockdown with or without 4 hours etoposide treatment and genomic DNA was isolated with DNeasy Blood and Tissue kit (Qiagen # 69504). Nucleic acid was measured by nanodrop ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested 4 days (96hr) after treatment and RNA extracted using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, USA). Concentrations of RNA samples were analysed using Qubit fluorometric assays (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: PC9 and CRUK0764 cells were immediately centrifuged at 300 × g for 4 min to remove supernatant and add 350 µl RLT buffer (Qiagen, 79216) containing 1% β-mercaptoethanol (Sigma ...
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bioRxiv - Plant Biology 2020Quote: Genomic DNA was extracted from n=192 rhizosphere and n=192 bulk soil samples using the DNeasy PowerSoil kit (Qiagen, Hilden, Germany). Paired-end sequencing of a 300-bp sequence spanning the V4 region of the ribosomal 16 S rRNA was generated using the Illumina MiSeq platform (Illumina Inc. ...
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bioRxiv - Epidemiology 2019Quote: ... Genomic DNA was extracted from female and male reproductive parts (RP, n = 1157) as well as from whole bodies (WB, n = 258) using DNeasy® Blood and Tissue Kit (Qiagen, Germany). We used flies from Pagirinya (PAG ...
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bioRxiv - Genomics 2022Quote: ... The reactions were incubated for 15 min at 60 °C and then terminated by adding 1 μl 5 M NaOH to degrade RNA and heating at 95 °C for 5 min followed by neutralization with 1 μl 5 M HCl and one round of MinElute column clean-up (Qiagen). The R1R DNA adapter was adenylated by using a 5’ DNA Adenylation kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2023Quote: ... 200 ng psiCHECK-2 reporter plasmid were co-transfected with 5 µl miScript miRNA mimics (Qiagen; CatNo. 219600) using 4 µl Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... antlers and faecal samples (n = 38) using Kit (QIAGEN), QIAamp® DNA Micro Kit (QIAGEN ...
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bioRxiv - Biophysics 2021Quote: ... purified N protein was incubated with RNAse A (Qiagen) with 1:15 (RNAse A ...
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bioRxiv - Microbiology 2020Quote: ... N RNA was purified using RNeasy mini kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... N mRNA was purified with RNeasy mini kit (Qiagen).
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bioRxiv - Bioengineering 2023Quote: ... and gDNA elimination columns (#74134, Qiagen, Germantown, MD n). RNA concentration and quality were assessed using a NanoDrop (#A38189 ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µg⋅ml –1 Leupeptin and 3 mM Benzamidine) with steel beads at 28.5 Hz for 1 min (Qiagen TissueLyser II ...
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bioRxiv - Microbiology 2021Quote: ... Blood was obtained for lymphocyte preparation 3-5 days after the fifth immunization and RNA was prepared from lymphocytes using the RNeasy kit (Qiagen, Valencia, CA). A VHH-display phage library was prepared essentially as described previously [70] following each of the rounds of alpaca immunization ...
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bioRxiv - Molecular Biology 2021Quote: ... EpiTect II DNA Methylation Enzyme Kit (335452) and EpiTect methyl II PCR system (EPMM104707-1A) for CpG methylation analysis was procured from Qiagen. [γ-32P]ATP was sourced from
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bioRxiv - Genomics 2023Quote: ... The samples were processed with Ovation RRBS Methyl-Seq System (NuGen) and bisulfite treatment was done with the Epitect Fast Bisulfite Conversion kit (Qiagen). RRBS libraries were controlled with a BioAnalyser (Agilent ...
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bioRxiv - Physiology 2020Quote: Total RNA was extracted from the gill samples collected from both Lake Winnipeg walleye (n = 10) and Delta Marsh walleye (n = 21) using the RNeasy Plus Mini Kit (Qiagen, Toronto, ON, CA) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... by shaking with two 3-mm glass beads for 2 min at 30 Hz with a Tissue-Lyser (Qiagen, Hilden, Germany). After 5 min in an ice-cold ultrasonic bath ...
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bioRxiv - Epidemiology 2020Quote: ... then ticks were washed by gentle shaking over 2-3 min at 7 Hz/s in a Tissue Lyzer (Qiagen, Germany). After discarding the supernatant ...
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bioRxiv - Immunology 2022Quote: ... The middle and inferior right lobes were weighted and homogenized with PBS (1:5 w/v) using a 5 mm stainless steel bead (Qiagen, USA) and a TissueLyser LT (Qiagen ...
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bioRxiv - Biophysics 2021Quote: ... The capture probes contained an 11- to 12-nt target-capturing sequence with 4 LNA residues (Tm = ~70°C, estimated using Qiagen web application) for high affinity and kinetically stable capturing of nucleic acid targets on the surface ...
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bioRxiv - Genomics 2020Quote: RNA was prepared from the 4-dpi flash-frozen spleen tissues of each of the above 20 chicks after homogenization in Qiazol reagent (Qiagen, Manchester, UK), and subsequent preparation using RNeasy RNA isolation kit (Qiagen ...
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bioRxiv - Genomics 2019Quote: ... 4°C) the aqueous phase was mixed with 700 μl 70% ethanol and applied to a Silica column (Qiagen RNAeasy Mini kit). RNA was then purified according to the manufacturers recommendations ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from whole fecal pellets or 50 μL of culture with a DNeasy PowerSoil HTP 96 kit (Qiagen 12955-4). 16S rRNA amplicons were generated using Earth Microbiome Project-recommended 515F/806R primer pairs using the 5PRIME HotMasterMix (Quantabio 2200410 ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from 1g of soil from 4 different samples from each sampled plot using the DNeasy PowerSoil Kit (Qiagen, Antwerp, Belgium) according with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 4 nmole standard desalted Ultramer™ DNA oligo from IDT) and PCR products (purified using QIAquick® PCR Purification Kit from QIAGEN) served as donor templates for small (3xFLAG epitope tag ...
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bioRxiv - Cancer Biology 2022Quote: ... 1,000 T-cells/CAR T-cells were FACS-sorted in single PCR tubes containing 4 ul of QIAGEN lysis buffer from QIAseq FX Single Cell RNA Library Kit (QIAGEN, Hilden, Germany) and stored at −80°C ...
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bioRxiv - Microbiology 2022Quote: Isolation of total DNA from a total of 107 transfected MOLT-4 cells was done using DNeasy Blood & Tissue Kit (Qiagen catalog # 69504). Isolated DNA was digested with either NcoI-HF (New England Biolabs # R0193 ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was applied to a nickel-NTA affinity column containing a total of 4 ml bed volume of nickel-NTA superflow resin (Qiagen, Venlo, Netherlands). After washing with a washing buffer containing 20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: ... spun down (400 g × 10’ at 4 °C) and the pellet was resuspended in 600μ of Qiazol Lysis Reagent (Qiagen, Cat.No. 79306) and kept at −80 °C before sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... step for 15 min at 4°C to remove insolubilized material and the supernatant was incubated with Ni-NTA agarose beads (Qiagen, cat: 30230) for 2 hrs ...
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bioRxiv - Physiology 2020Quote: ... Sections were then post-fixed in 4% PFA/PBS before washing with buffer containing Proteinase K (PK; Qiagen UK Ltd, Manchester, UK) (1 µg/ml PK ...
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bioRxiv - Immunology 2021Quote: ... Samples were centrifuged at 14,000 g at 4°C for 10 mins and the supernatant collected for processing using a RNeasy mini-isolation kit (Qiagen Ltd, UK) according to the manufacturers’ protocol ...
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bioRxiv - Immunology 2020Quote: ... Vγ4+TCRγδ+CD3+CD44+CD27− and Vγ4−TCRγδ+CD3+CD44+CD27− populations from each mouse strain were sorted using a FACSAriaIII: populations into RNA protect (QIAGEN, Hilden, Germany). Total RNA was extracted from the sorted cells according to the “Purification of total RNA from animal and human cells” protocol of the RNeasy Plus Micro Kit (QIAGEN) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then cultured for an additional 4 weeks and genomic DNA (gDNA) was isolated using DNeasy Blood and Tissue Kit (Qiagen, Cat. # 69506). sgRNA sequences were PCR amplified from gDNA using Platinum SuperFi II DNA Polymerase (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: Total RNA were extracted from the basal stem section of mature Col-0 and msil2/4 mutant plants using the RNeasy Plant Mini Kit (Qiagen, Cat.#74904) and treated onto the column with the RNase-Free DNase Set (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA and RNA from patient tumor tissue biopsies and mouse tumors were extracted from 4 sections of 10 µm each from FFPE tumor blocks using the AllPrep® DNA/RNA FFPE kit (Qiagen #80234) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation at 20,000 x g for 45 minutes at 4°C and incubated with Ni-NTA agarose resin (Qiagen, catalogue number: 30230) pre-equilibrated with lysis buffer for 2 hours at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... a total of 600 µL of cold 2:1 methanol:chloroform was added and homogenized in a bead-based tissue homogenizer at 25 Hz for 4 minutes (TissuLyser II, Qiagen, Hilden, Germany). 200 µL each of water and chloroform were then added ...
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bioRxiv - Plant Biology 2023Quote: ... leaf discs (30 mg) were collected 4 dpi and then flash frozen in liquid nitrogen and homogenized with the Tissue Lyser II (QIAGEN, Hilden, Germany) to make fine powder at 300 rpm for 1 min ...
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bioRxiv - Genomics 2024Quote: ... the Qiagen RNeasy Mini Kit was used according to Qiagen RNA Protect Reagent Handbook Protocols 4 and 7 with Appendix B on-column DNase digestion (Qiagen, Hilden, Germany). The RNA was assessed with UV-Vis spectrophotometry (Denovix DS-11 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Quality of the final library has been evaluated through Qubit 4 and fragments sizes were checked by migration using QIAxcel Advanced Instrument (QIAgen, Hilden, Germany). Fragments ready for sequencing sized between 280bp and 320bp ...
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bioRxiv - Cancer Biology 2024Quote: ... Targeted knockdown was conducted with 100nM ON-TARGETplus smartpool human c-MYC (mixture of 4 individual siRNA) and/or human KLF6-SV1 siRNA (Dharmacon, Lafayette, CO) with HiPerfect (Qiagen, Valencia, CA).
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bioRxiv - Bioengineering 2024Quote: Whole mouse aortas (n=18 per group) cleaned of perivascular tissue were perfused with sterile HBSS and stabilized overnight at 4◦C in RNAProtect reagent (Qiagen; Hilden, Germany) before long-term storage at -80◦C ...