Labshake search
Citations for Qiagen :
701 - 750 of 5865 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... SiCBL4 and SiCBL7 promoters were respectively amplified using biotin-labeled primers (Table S1) synthesized by Sangon Biotech (Shanghai China) and purified using a PCR purification kit (Qiagen). EMSA was conducted using a LightShift Chemilumniescent EMSA Kit (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... Integration of the repair construct into the genome was verified by diagnostic PCR using primers 40-43 on parasite genomic DNA extracted using the DNeasy Blood and Tissue Kit (Qiagen).
-
bioRxiv - Microbiology 2023Quote: ... The primers were used in a 28 cycle PCR with the HotStarTaq Plus Master Mix Kit (Qiagen Inc., Hilden, Germany). The PCR cycle conditions were as follows ...
-
bioRxiv - Cell Biology 2024Quote: ... Actin was used as a housekeeping gene to normalize the amount of viral RNA by using the qPCRBIO SyGreen mix HI-ROX (PCR Biosystems) together with QuantiTect primer assay (QT01680476, Qiagen). All Quantitative PCR (qPCR ...
-
bioRxiv - Cancer Biology 2019Quote: ... and qPCR was performed using gene specific QuantiTect Primer Assay primers from Qiagen. Relative expression levels were normalized to gapdh expression according to the formula <2^− (Ct gene of interest − Ct gapdh ...
-
bioRxiv - Cancer Biology 2020Quote: ... and internal control EIF3D and RPL13A specific primers (RT2 qPCR Primer Assays -Qiagen) and RT² SYBR® Green qPCR master mix using the recommended protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... Primers used against DDIT3 (CHOP) were commercially available QuantiTect primer assays (Qiagen, UK). Please refer to table S2 and S3 in the supplementary material for the sequences of other primers used ...
-
bioRxiv - Plant Biology 2020Quote: The real-time quantification of cDNA corresponding to 2µg of total RNA was performed in the ABI PRISM 7900HT sequence detection system using the QuantiTech SYBR Green RT-PCR kit (Qiagen). The Gene-specific primers used are listed in Table S1 ...
-
bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR E assay12 using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
-
bioRxiv - Plant Biology 2020Quote: Total RNA was extracted by quantitative RT-PCR from Col-0 and DWF4-OE seedlings 6 days post germination with the RNeasy Mini Kit (Qiagen). For DWF-OE ...
-
bioRxiv - Microbiology 2021Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR assay (24, 25) using the QuantiFast probe kit (Qiagen) according to instructions of the manufacturer ...
-
bioRxiv - Genetics 2022Quote: SARS-CoV-2 RNA copy number was quantified in RNA samples extracted from infected cells using the QuantiTect probe RT-PCR Kit (Qiagen). TWe prepared a standard curve using the ORF9 nucleoprotein coding sequence cloned in pcDNA3 ...
-
bioRxiv - Microbiology 2020Quote: ... cDNA was then diluted 25 times to serve as template for real-time quantitative PCR (RT-qPCR) using Rotor-Gene 6000 (Corbett Research, QIAgen), as previously described (Forlenza et al. ...
-
bioRxiv - Microbiology 2021Quote: ... 10 µl of the cell suspension from each dilution was dispensed into 24 wells of a 96 well plate containing 2 μl One-step RT-PCR enzyme (Qiagen), 10 μl 5x One-step RT-PCR buffer (Qiagen) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... reverse transcription and amplification of the S gene were done with the one-step QuantiFast Sybr Green RT-PCR mix (Qiagen) as follows ...
-
bioRxiv - Microbiology 2020Quote: The 16 h clindamycin-induced and uninduced seed cultures were cultivated and incubated in 1 ml of RNA Protect in the same manner as for RT-PCR Total RNA was extracted with an RNeasy RNA isolation kit (Qiagen). The isolated RNA was treated with DNaseI (0,1 U μl-1 ...
-
bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR assay (Corman et al., 2020; Wolfel et al., 2020) using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
-
bioRxiv - Microbiology 2020Quote: ... The SVIP-MPv2 assay was developed and validated as a one-step procedure using the QuantiNova Probe RT-PCR Kit (Qiagen) with final concentrations of primers (1.4μM each ...
-
bioRxiv - Bioengineering 2021Quote: ... Total RNA (5 μl) was used for cDNA synthesis and qPCR was performed in one step using QuantiTect Probe RT-PCR (Qiagen) on a StepOnePlus System (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Gene expression was evaluated by qPCR using the ONE-step QuantiTect Probe RT-PCR Kit (Qiagen, Cat No./ID: 204445). For each reaction 2 μL of RNA were used ...
-
bioRxiv - Developmental Biology 2020Quote: ... and 10ng of RNA per sample was used in each reaction according to instruction in QuantiFast SYBR Green RT-PCR kit (QIAGEN). Samples were subsequently loaded on to a LightCycler 480 Instrument II (Roche) ...
-
bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
-
bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR E assay33 using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
-
bioRxiv - Pathology 2021Quote: ... and gene expression was carried using the RT² Profiler PCR Array for human cellular stress responses (Qiagen, Catalog#:PAHS-019ZA) following manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... reverse transcription and amplification of the S gene were performed using the one-step QuantiFast Sybr Green RT-PCR mix (Qiagen) as follows ...
-
bioRxiv - Pathology 2021Quote: ... were premixed and added to each well of RT² Profiler™ PCR Array Mouse NFκB Signaling Pathway (Qiagen, PAMM-025Z) for target gene qPCR ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... RNA from the original material used for next-generation sequencing of the serum and passage 3 cultured MRI103 virus was used as the template for amplification using the One-Step Ahead RT-PCR kit (Qiagen).
-
bioRxiv - Immunology 2021Quote: ... 5 μl RNA was used in a one-step real-time RT–PCR E assay using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
-
bioRxiv - Immunology 2021Quote: ... Five μl RNA was used in a one-step real-time RT-PCR using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
-
bioRxiv - Molecular Biology 2021Quote: ... RT-qPCR was performed as previously described9 with miRCURY LNA RT and SYBR Green PCR miRNA kits and miRCURY LNA probes for miRNAs (Qiagen) and High-capacity cDNA transcription kit and Fast SYBR Green (Qiagen ...
-
bioRxiv - Immunology 2021Quote: Cytokines and cellular sensors were measured in neonatal lung homogenates (cDNA prepared as per viral titres) by RT-qPCR using QuantiFast SYBR Green PCR Kit (Qiagen) and QuantiTect Primer Assays (Qiagen ...
-
bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed with a primer set targeting partial regions of the ORF1b gene in the SARS-CoV-2 virus using the QIAGEN OneStep RT-PCR kit (Qiagen) as previously reported (51) ...
-
bioRxiv - Bioengineering 2023Quote: Reverse transcription of isolated miRNAs for quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed with a miScript II RT Kit (Qiagen) for selected miRNA targets ...
-
bioRxiv - Microbiology 2023Quote: A 388 bp region surrounding NS4A position 19 was amplified from total RNA extracted from a subset of brains using OneStep RT-PCR (Qiagen) using the following primers ...
-
bioRxiv - Microbiology 2024Quote: ... reverse transcription quantitative PCR (RT-qPCR) assays were performed with the QuantiTect Virus + ROX Vial Kit (Qiagen, Toronto, ON, Canada) in a LightCycler® 480 system (Roche Molecular System) ...
-
bioRxiv - Cancer Biology 2021Quote: ... DNase (RNase-Free DNase Set, Qiagen, catalog#:79254) was used to degrade DNA during the extraction as outlined in the RNeasy Mini Handbook ...
-
bioRxiv - Genetics 2021Quote: ... with RNase-Free DNase Set (Qiagen no. 79254) according to the manufacturer instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... and the RNase-Free DNase Set (#79254; Qiagen). The isolated heart RNA was converted into cDNA by QuantiTect Reverse Transcription Kit (#205311 ...
-
bioRxiv - Immunology 2022Quote: ... treated with DNase (RNase-Free DNase Set, Qiagen), and reverse transcribed to generate cDNA with High-Capacity RNA-to-cDNA™ Kit (Themo-Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... RNase-Free DNase Set (Qiagen, cat no. 79254) was used for further DNase digestion ...
-
bioRxiv - Plant Biology 2019Quote: ... and treated with RNase-Free DNase Set (QIAGEN). Reverse transcription was carried out using the SuperScript IV Reverse Transcriptase (ThermoFisher) ...
-
bioRxiv - Neuroscience 2019Quote: ... or with RNase-Free DNase Set (Qiagen, #79254) (larval OSN nuclei samples) ...
-
bioRxiv - Microbiology 2020Quote: ... was treated with RNase-Free DNase Set (Qiagen) according to the manufacturer’s protocol to remove possible traces of genomic DNA.
-
bioRxiv - Genetics 2021Quote: ... and treated with RNase-free DNase Set (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2020Quote: ... Following DNase treatment (RNAseq-Free DNAse Set (Qiagen)) and RNA purification (RNeasy MinElute Kit) ...
-
bioRxiv - Plant Biology 2021Quote: ... along with the RNase-free DNase Set (Qiagen). We constructed sequencing libraries with the KAPA Stranded mRNA-seq Kit (KAPA Biosystems ...
-
bioRxiv - Microbiology 2019Quote: ... and the DNAse RNase-Free Set (Qiagen, Netherlands) according to manufacturer’s instructions and as described previously (12) ...
-
bioRxiv - Plant Biology 2021Quote: ... and treated with RNase-Free DNase Set (QIAGEN). Reverse transcription was carried out using the SuperScript IV Reverse Transcriptase (ThermoFisher) ...
-
bioRxiv - Genetics 2020Quote: ... in conjunction with RNase-Free DNase Set (Qiagen). cDNA was generated using the High-Capacity RNA-to-cDNA™ Kit (Applied Biosystems).
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... combined with the RNase-Free DNase Set (Qiagen). We stored RNA at -70°C ...