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Citations for Qiagen :
701 - 750 of 863 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Samples were analyzed by NTA and then processed with AllPrep DNA/RNA/Protein Mini Kit (cat. no. 80004, Qiagen).
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bioRxiv - Microbiology 2023Quote: ... Total RNA was isolated from these samples according to the manufacturer’s instruction using AllPrep DNA/RNA/Protein Mini Kit (Qiagen). RNA was quantified using a Nanodrop-1000 spectrophotometer (Thermo-Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from hippocampus and pre-frontal cortex was purified with AllPrep DNA/RNA/Protein Mini Kit (QIAGEN, 80004) and DNAseI digested (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... the soluble fraction containing the His-VitR protein was purified using NTA-resin affinity chromatography in phosphate buffer according to manufacturer’s recommendations (Qiagen). After concentration (Vivaspin 6 Concentrator ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was isolated using the SampleIN Direct PCR Kit (highQu) or the AllPrep DNA/RNA/Protein Mini Kit (Qiagen), and amplified with ALLin HS Red Taq Mastermix (highQu ...
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bioRxiv - Biophysics 2024Quote: ... cleaved protein was filtered using a 0.45 μm syringe filter and purified by a second Ni–NTA purification step (Qiagen). As a final purification step ...
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bioRxiv - Developmental Biology 2020Quote: ... antibody-precipitated input DNA were purified with minElute reaction cleanup kit (Qiagen, #28204), reconstituting to 35 uL EB buffer.
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bioRxiv - Biochemistry 2021Quote: Assay mixes consisted of 25 nM ALEXA488-conjugated penta-His antibody (Qiagen # 35310), 50 μM DTT ...
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bioRxiv - Microbiology 2023Quote: ... Mouse monoclonal antibody that recognizes the strep-tag epitope was purchased from Qiagen. Recombinant NifB expressed in E ...
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bioRxiv - Biophysics 2021Quote: The libraries midi-preps were digested with HindIII and NheI restriction enzymes and the insert containing the mutated protein was gel purified (MinElute Gel Extraction Kit, QIAGEN) to be later cloned into the two assay plasmids by temperature-cycle ligation.
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bioRxiv - Cell Biology 2022Quote: ... were performed on significantly altered proteins in CAVIN1-null cells using the “core analysis” function included in the Ingenuity Pathway Analysis (IPA) software (QIAGEN Bioinformatics ...
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bioRxiv - Molecular Biology 2020Quote: RNA and protein were extracted from cell lines using the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen, Valencia, CA, USA). Total RNA was also DNase treated using the TURBO DNA-free Kit (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: RNA was extracted simultaneously from the same tissue pieces used for DNA extraction using AllPrep DNA/RNA/Protein Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The his-tagged 3C protease was removed by flowing and washing off cleaved transporter protein over two 100 µl beds of Ni-NTA Superflow resin in tandem (Qiagen). Eluent was concentrated using 100 kDa cut-off Amicon Ultra filters (Millipore-Sigma).
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bioRxiv - Microbiology 2021Quote: ... Proteins were purified under denaturing conditions by nickel-nitrilotriacetic acid (NTA)-agarose affinity chromatography as described by the manufacturer (Qiagen). Polyclonal rabbit antisera were raised by Covance Research Products Inc ...
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bioRxiv - Biochemistry 2021Quote: ... Cell debris and protein aggregates were removed by centrifugation and the supernatant was loaded on a Ni-NTA Superflow affinity resin (Qiagen). The resin was washed with lysis buffer and the protein eluted with lysis buffer containing 200 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: Genomic DNA from 1×106 FACS sorted p7 MPs from one animal was isolated with the All Prep DNA / RNA / Protein Mini Kit (Qiagen) and used as one sample ...
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bioRxiv - Biochemistry 2020Quote: ... Cell debris and protein aggregates were removed by centrifugation and the supernatant was loaded on a Ni-NTA Superflow affinity resin (Qiagen). The resin was washed with lysis buffer and the protein eluted with lysis buffer containing 200 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA from CRISPR-on HEK293T cells and control cells were purified using a AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Then ...
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bioRxiv - Microbiology 2020Quote: ... The soluble fraction containing the protein of interest was obtained by centrifugation at 12000xg for 20 min and was mixed with Ni2+-NTA beads (Qiagen) for 1.5 h by rotation at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed 3 times with sterile 1x PBS and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed four times.
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bioRxiv - Biochemistry 2022Quote: ... Metal-chelate affinity chromatography was used to purify the 6xHis-tagged proteins using the Ni-NTA Fast Start Kit (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Plasmids encoding the recombinant full-length Spike protein and the RBD were provided by F. Krammer (Mt. Sinai) and purified by nickel-nitrilotriacetic acid resin (Qiagen). ELISA plates (Immulon 4 HBX ...
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bioRxiv - Microbiology 2022Quote: ... the His-ChuP protein was purified from the soluble extract by affinity chromatography in a Ni-NTA Superflow column (Qiagen). The elution fractions were evaluated using 18% SDS-PAGE ...
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bioRxiv - Biophysics 2022Quote: ... Supernatants were incubated with glutathione Sepharose (GSH) fast flow beads (GE-Healthcare) for GST-tagged proteins or nickel– nitriloacetic acid (Ni-NTA) agarose (Qiagen) for His-tagged proteins for 2 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... the samples were centrifuged 15,000 x g at 4°C for 30 min and the resulting supernatant containing the target protein was filtered and purified using a pre-equilibrated Ni-NTA beads column (Qiagen). The recombinant protein was eluted using 4 mL of elution buffer (8 mM Na2HPO4 ...
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bioRxiv - Biochemistry 2021Quote: ... imidazole (20 mM final) was added to the eluted protein which was subsequently incubated with 5 mL NiNTA resin (Qiagen) at 4 °C overnight with constant inversion ...
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bioRxiv - Biochemistry 2021Quote: ... 6XHis tagged-Trx1 and Trr1 recombinant proteins were cloned in PET24 vectors and purified by column affinity onto Ni-NTA beads (Qiagen). The precursor was imported in 50 μg of the indicated yeast mitochondria in the presence of 2 mM ATP and 2.5 mM NADH at 30°C ...
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bioRxiv - Neuroscience 2020Quote: ... total RNA was extracted from AAV1-transduced primary hippocampal neurons (DIV12-14) using an AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Total RNA was polyA selected and sequenced (Hudson Alpha GSL ...
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bioRxiv - Biochemistry 2022Quote: ... Cell debris were removed by centrifugation (35,000 g at 4°C for 20 min) and the N-terminal His-tagged proteins were purified from the supernatant using NiNTA agarose (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes containing the channels were collected by centrifugation (100,000×g) and proteins were extracted with 40 mM DM.65 Channels were purified using Ni-NTA agarose beads (Qiagen). Liposome reconstitution was performed using E ...
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bioRxiv - Bioengineering 2021Quote: The dataset of differentially abundant proteins between groups were classified for cell compartment association with Ingenuity Pathway Analysis (IPA, Qiagen) and for matrisome categories using The Matrisome Project database,[28] ...
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bioRxiv - Cell Biology 2021Quote: ... Identified interacting proteins were analyzed through the use of QIAGEN’s Ingenuity® Pathway Analysis (IPA®, QIAGEN, Redwood City, CA) and UniProt online protein knowledgebase (http://www.uniprot.org/).
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bioRxiv - Cell Biology 2021Quote: The GST fusion proteins were expressed in bacteria (E. coli, BL21) and purified by glutathione-Sepharose (Cat No. 27-4574-01, Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... We infected Sf9 cells with the recombinant virus to express and purify the His-tagged proteins using a nickel-nitrilotriacetic acid (Ni-NTA, Qiagen) column ...
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bioRxiv - Immunology 2021Quote: ... Plasmids encoding the recombinant full-length Spike protein and the RBD were provided by F. Krammer (Mt. Sinai) and purified by nickel-nitrilotriacetic acid resin (Qiagen). ELISA plates (Immulon 4 HBX ...
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bioRxiv - Molecular Biology 2021Quote: The assessment of ligand binding was performed via shifts in protein thermostability upon binding using thermal denaturation on a rotary quantitative PCR (qPCR) instrument (Qiagen Rotor-Gene Q 2plex HRM ...
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bioRxiv - Cell Biology 2021Quote: ... and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed in triplicate.
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 ELISA was developed in-house using His tagged proteins bound on Ni-NTA HisSorb Strips or Plates (Qiagen). ELISA assay on mouse sera were performed with His-tagged SARS-CoV-2 full length Spike protein (produced in baculovirus ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted at low speed (~1000 RPM) and lysed with one mL RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen).
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bioRxiv - Biophysics 2020Quote: ... 45 min at 4 °C) and supernatant containing His-tagged proteins were purified by nickel-nitrilotriacetic acid (Ni-NTA) purification (Qiagen). Protein was eluted in a final elution buffer of 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was supplemented with 10 mM imidazole and His-tagged proteins were purified by affinity chromatography on NiNTA column (Qiagen) at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... 250,000 cells were lysed in RLT-plus buffer and RNA was purified with AllPrep DNA/RNA/Protein Mini Kit (#80004, Qiagen) according to the manufacturer protocol ...
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bioRxiv - Biophysics 2022Quote: ... membranes were solubilized in 1.2% N-decyl-β-maltoside (DM) and the protein was purified on a Ni-NTA affinity column (Qiagen). Following His-tag removal ...
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bioRxiv - Microbiology 2022Quote: ... Gene expression of 84 heat shock genes in mock-infected or 229E-infected cells was analyzed using Human Heat Shock Proteins & Chaperones RT2 Profiler PCR array (Qiagen). Real-time PCR analyses were performed with specific primers ...
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bioRxiv - Systems Biology 2022Quote: The co-immunoprecipitated RNA samples were submitted to protein digestion using proteinase K (Fermentas) and purified using the MinElute Reaction Cleanup Kit (QIAGEN) with a DNase treatment step ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumour samples were fresh-frozen in liquid nitrogen and stored at -80°C for protein isolation or preserved in RNAlater solution (Qiagen) for RNA isolation ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were collected by double centrifugation and 6x His-tagged target protein in the supernatant was purified using the medium by Ni-NTA (Qiagen) affinity chromatography ...
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bioRxiv - Bioengineering 2024Quote: ... The output of differentially expressed proteins was further investigated using Ingenuity Pathway Analysis® (IPA; Qiagen®, Redwood City, USA). Data was also independently analysed using PEAKS studio (Bioinformatics Solutions® Inc. ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins harvested after 6 days post transfection were purified from the supernatant on a Ni-NTA Superflow column (#30430, Qiagen). Immediately after the elution ...