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Citations for Qiagen :
601 - 650 of 863 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the soluble fraction was used for the purification of heterologous proteins using Ni-NTA agarose resin (QIAGEN), previously equilibrated with equilibration buffer (200 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... Dephosphorylated proteins were then further purified by the C-terminal His-tag using Ni-NTA resin (Qiagen). Full-length dephosphorylated proteins were subsequently purified by size-exclusion chromatography on a HiLoad 16/600 Superdex 200 prep grade column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... The identified proteins were then subjected to annotate the biological functions by Ingenuity Pathway Analysis (IPA, Qiagen). Molecules from the dataset that met the cutoff of the Ingenuity Knowledge Base were considered for the analysis ...
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bioRxiv - Cell Biology 2024Quote: ... Secreted proteins were purified from culture media using 100 μl of 50% slurry of Ni-NTA (Qiagen). After overnight rotation at 4°C ...
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bioRxiv - Immunology 2020Quote: ... immunoplates were coated with 0.125ug of Tetra-His antibody (34670; QIAGEN) followed by 2 ug/ml and 5 ug/ml of soluble RBD and NP ...
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bioRxiv - Cell Biology 2021Quote: ... and His6- Vps4 was detected using Penta-His Antibody (Qiagen, Germany) per manufacturer instruction ...
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bioRxiv - Biophysics 2020Quote: ... anti-His antibodies from the Penta-His HRP Conjugate Kit (Qiagen) were used.
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bioRxiv - Microbiology 2022Quote: ... Antibodies against His-tag and S-tag were purchased from Qiagen and GenScript respectively ...
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bioRxiv - Biochemistry 2022Quote: ... anti-His antibodies from the Penta-His HRP Conjugate Kit (Qiagen) were used ...
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bioRxiv - Microbiology 2020Quote: ... An anti·His antibody conjugated to horseradish peroxidase (Penta·His HRP Conjugate, Qiagen) was used to detect 6xHis-tagged proteins ...
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bioRxiv - Cell Biology 2021Quote: ... was from List Biological Laboratories and the mouse monoclonal antibody against the His-tag from Qiagen.
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bioRxiv - Biophysics 2022Quote: The following anti-His fluorophore-conjugated antibodies were purchased from Qiagen and used in UV-Bind assays for His-tagged proteins ...
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bioRxiv - Biochemistry 2023Quote: ... Bound PEX5L was detected immunologically using monoclonal Anti-His Antibodies (Qiagen).
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bioRxiv - Biophysics 2020Quote: ... proteins were expressed in Escherichia coli strain BL21 (DE3) and purified via nickel-nitrilotriacetic acid affinity chromatography (Qiagen) followed by ion exchange chromatography on an Äkta system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: Purified recombinant poly-histidine protein was obtained by FPLC under native conditions using a Ni-NTA column (Qiagen), and a Biologic DUO-FLOW BIO-RAD chromatography system (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... microdissected human brain tissue was flash frozen and lysed to extract proteins using a TissueLyser II system (QIAGEN) and total protein concentration determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific 23225) ...
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bioRxiv - Molecular Biology 2021Quote: ... The expressed EcoCas3-tag fusion proteins were purified using nickel affinity resin (Ni-NTA, Qiagen, Hilden, Düsseldorf, Germany). To remove tags ...
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bioRxiv - Neuroscience 2020Quote: ... DNA and RNA were extracted using the Qiagen AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany; #80004). The standard manufacturer’s protocol was followed to extract DNA and RNA from each cell pellet ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was isolated from dissected tissues using an AllPrep DNA/RNA/Protein Mini kit (Qiagen, Valencia, CA). The concentration and purity were determined using a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then purified on Ni2+ -NTA affinity resin following a modified version of the manufacturer’s instructions (QIAGEN) [25] ...
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bioRxiv - Microbiology 2020Quote: ... The 6 × his-tagged proteins were purified using a nickel-nitrilotriacetic acid (Ni-NTA) agarose matrix (30250, Qiagen). Cell-free extracts were loaded on 0.5 ml Ni-NTA matrixes and incubated on a roller shaker for 2 h at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Protein lysates were separated on NuPage 5-12% Bis-Tris or 3-8% Tris-Acetate gels (Novex, Qiagen) using MES running buffer (Novex ...
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bioRxiv - Plant Biology 2020Quote: ... The His-tagged proteins were purified by Ni-affinity gravity-flow chromatography using the Ni-NTA agarose (Qiagen) following the product manual.
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged ubiquitin-conjugated proteins were purified by adding 100 µL of 50 % Ni-NTA agarose beads (Qiagen) equilibrated in A2 buffer to 1 mL of cleared extracts and incubated with rotation for 2-4 hr at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... the tags and protease were removed from the protein by passage through a Ni-NTA superflow cartridge (QIAGEN). The protein was further purified by size exclusion chromatography on a Superdex 200 Increase (Cytiva ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His protein was purified from cell culture supernatant using a Ni-NTA (Qiagen) affinity column ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from the frozen placental tissue using the Protein and RNA Isolation (PARIS) kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The phage protein capsid was digested with Proteinase K and the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) was used to purify the DNA ...
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bioRxiv - Bioengineering 2020Quote: Initial small test purifications were performed using Ni-NTA Spin Column for His-Tagged proteins (Qiagen, Hilden, Germany) following the protocol for 6xHis-Tagged Proteins under Native Conditions from E ...
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bioRxiv - Biochemistry 2020Quote: ... The Ni-NTA agarose resin for purification of his-tagged proteins was purchased from Qiagen (Germantown, MD, USA). Superdex 75 16/600 and Superdex 200 Increase 10/300 size exclusion chromatography columns were purchased from GE Healthcare (Uppsala ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was isolated from 5 × 105 cells with the AllPrep© DNA/RNA/Protein Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was extracted from samples using the AllPrep DNA/RNA/Protein Mini Kit (80004, Qiagen Sciences, Inc.) and 0.25 μg of total RNA was reverse transcribed by SuperScript™ IV First-Strand cDNA Synthesis System (18091050 ...
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bioRxiv - Immunology 2021Quote: ... to provide Uniprot entries and further processed in Perseus (v1.6.6.1).18–20 Significantly regulated proteins were investigated in Ingenuity Pathway Analysis (v47547484, Qiagen, USA)
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bioRxiv - Developmental Biology 2021Quote: Log fold-change values of the top 5% proteins were used as input for Ingenuity Pathway Analysis (Qiagen: https://digitalinsights.qiagen.com/products/features/) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were disrupted by sonication and N-terminus His6-tagged proteins were purified on Ni-NTA columns (Qiagen) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins NHis6-Fnr1 and Fnr3-CHis6 in the supernatant were purified on Ni2-NTA resin (Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... and the cleaved His6-MBP tag was removed by passing the protein through an amylose resin column (Qiagen). The flow-through fraction containing the tag-free protein was further purified using a Superdex 200 (10/300 GL ...
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bioRxiv - Biophysics 2021Quote: ... and the protein of interest was purified by affinity chromatography on a Ni-NTA column (Qiagen, Hilden, Germany), using a TRIS-HCl 50 mM ...
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bioRxiv - Microbiology 2022Quote: ... filtered and the secreted recombinant proteins were purified by affinity chromatography using Ni-NTA resin (Qiagen; Cat.# 30230). Specifically ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted using an imidazole gradient and subsequently loaded onto a 5 ml StrepTactin Superflow Cartridge (Qiagen) at a flow rate of 0.8 ml/min ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from samples using Qiagen’s AllPrep Bacterial DNA/RNA/Protein Kit (Cat# 47054; QIAGEN, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The agarose gel bound with the His-tagged recombinant protein was packed in the chromatography column (Qiagen, Germany), and the flow through was discarded ...
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bioRxiv - Plant Biology 2023Quote: ... All protein sequences (for accession numbers see Supplementary Table 1) were aligned using CLC Genomics Workbench v20.0.4 (Qiagen) and the phylogenetic tree was constructed with the neighbor-joining method using 1000 bootstrap replicates ...
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bioRxiv - Microbiology 2022Quote: ... The cells were collected by centrifugation and the recombinant protein was column-purified using Ni2+-NTA resin (Qiagen). The purified protein was stored at -80°C ...
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bioRxiv - Microbiology 2023Quote: ... The N-terminal His-tagged Sif protein (Lmo0946-His6) was expressed and purified using Ni-NTA resin (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... or 0.2 or 0.4 μL of the protein solution and 0.2 μL of a series of commercially available crystallization screens (Qiagen). Conditions that provided initial hits were optimized using bespoke screens prepared at the crystallization facility ...
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bioRxiv - Immunology 2023Quote: DNA was extracted from samples using Qiagen’s AllPrep Bacterial DNA/RNA/Protein Kit (Cat# 47054; QIAGEN, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Hippocampi were placed in protein low-binding tubes mounted onto a Qiagen TissueLyser II (Qiagen cat. Nr. 85300). Lysates were span down at 20.000 rcf for 20 minutes at 4°C to remove debris ...
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bioRxiv - Cancer Biology 2023Quote: Enrichment analysis was performed both on the differentially expressed genes and proteins through the use of IPA (QIAGEN Inc. ...
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bioRxiv - Immunology 2023Quote: ... sdAb and proteins from bacterial cell lysates (SLP and TXA1) were purified with Ni-NTA resin (Qiagen: 30210), using Tris Buffered Saline (TBS ...