Labshake search
Citations for Qiagen :
701 - 750 of 10000+ citations for Fumarase from porcine heart since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... RNA was isolated from Hela cells harvested from confluent cultures according to the manufacturer’s instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA). RNA was used as a template for reverse-transcriptase polymerase chain reaction (RT-PCR ...
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bioRxiv - Microbiology 2022Quote: ... Positive plasmids were extracted from overnight liquid cultures obtained from replica colonies by using the Plasmid Mini kit (Qiagen, Germantown, MD), and two to five clones for each strain were sequenced to ensure sequence fidelity to the previously cloned templates (35) ...
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bioRxiv - Genetics 2022Quote: Total RNA was isolated from 50-100μl of packed worms from indicated genotypes/conditions using the RNeasy Mini Kit (Qiagen, Catalog #74104) and QIAshredder (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: mtDNA deletions were initially detected by long-range PCR (LR-PCR) from DNA extracted from cultured fibroblasts using DNeasy blood and tissue kit (Qiagen #69504) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The homology arms were PCR amplified from genomic DNA extracted from wildtype flies using the DNeasy Blood and Tissue Kit (Qiagen #69506), while the gRNAs were PCR amplified using the pHACK-QF2 backbone as a template ...
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bioRxiv - Neuroscience 2021Quote: RNA samples from WT and Rlip+/- mouse liver and brain were prepared using the RNeasy mini kit from Qiagen (Valencia, CA) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: DNA was isolated from both fresh and frozen organoid cultures utilizing the DNeasy Blood & Tissue Kit from Qiagen (Cat. No. 69504). Once DNA was isolated from the organoid cultures ...
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bioRxiv - Microbiology 2021Quote: DNA from biopesticide products was extracted directly and from Luria-Bertani cultures using Qiagen’s DNeasy DNA Extraction kit (Qiagen NV, Germany) with a modified protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: Total RNA was extracted from pools of three whole specimens per site from nine different locations using RNeasy Mini Kit (Qiagen, USA). Quality and integrity of extracted RNA was assessed using Bioanalyzer 2100 (Agilent ...
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bioRxiv - Cell Biology 2020Quote: Genomic DNA was isolated from BMBMCs from 20 active ITP patients using a QIAamp DNA Blood Mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... DNA was isolated from 2 g of homogenized material from frozen needles using the DNeasy Plant Maxi Kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... DNA was extracted from fecal pellets from male and female mice using the QIAamp DNA Stool Mini Kit (QIAGEN, Hilden, Germany) and homogenized using 0.1 mm zirconia/silica beads (Biospec ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from dissected CD31+ BECs from the mouse brain using an RNA extraction kit (#74104, Qiagen, MD, USA). The extracted RNA was stored in RNAse-free water at -80°C until transfer to the Boston University School of Medicine Microarray Core Facility ...
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bioRxiv - Microbiology 2021Quote: Total DNA was extracted from the sediment samples from each SH site using the DNeasy PowerSoil Kit (Qiagen Inc., Hilden, Germany) following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... the livers were perfused 45 hr post mosquito-bite challenge with 1× PBS and total RNA was extracted from the lower right caudate process of the caudate lobe of the liver from each mouse using miRNeasy kit (Qiagen: 217004). The complementary DNA (cDNA ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was extracted from MIEV-or PKCα-KR-transduced cells from day 17-23 co-cultures using RNeasy mini kit (Qiagen, UK) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from fibroblasts obtained from each individual mouse from each of the five non-CC inbred strains using AllPrep DNA/RNA Mini kit (Qiagen, #80204). The concentration and purity of extracted DNA was determined using a NanoDrop 2000 UV-vis spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... DNA was isolated from the cells according to the quick-start protocol from the DNeasy® Blood and Tissue Kit (Qiagen). PCR primers used are listed in Supplementary table 1.
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bioRxiv - Microbiology 2023Quote: Total DNA from each time point of experimental microbial communities was extracted from samples using QIAamp DNA mini kit (Qiagen, Germany) following the manufacturer’s instructions ...
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bioRxiv - Zoology 2022Quote: Total genomic DNA was extracted from a tissue sample from ethanol-preserved specimens and DNA was purified using a DNeasy Blood & Tissue Kit (Qiagen, Hilden), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA and chromatin-associated RNA from HeLa cells and total RNA from differentiating motor neurons were extracted using the miRNeasy kit (Qiagen, 217004) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: For ROCK inhibition experiments total RNA was extracted from >1000 sea urchin embryos in each condition using RNeasy Mini Kit (50) from QIAGEN (#74104) according to the kits’ protocol ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from whole frozen renal tissue from wild-type and mutant mice and purified with RNeasy Plus Mini Kit (Qiagen, 74134). Ribosomal RNA was removed from 1 μg of total RNAs ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted from the soil or the suspensions from the reactors using DNeasy PowerSoil Pro Kit (Qiagen, Hilden, Germany) according to the protocol provided by the manufacturer ...
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bioRxiv - Genomics 2023Quote: High molecular weight was extracted from approximately 20mg of cortex tissue from each donor using either the MagAttract HMW DNA kit (Qiagen 67563) or Circulomics Nanobind Tissue kit (Pacbio 102-302-100) ...
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bioRxiv - Cancer Biology 2023Quote: ... Regions of overlap were macrodissected from the intervening sections and RNA was extracted using AllPrep DNA/RNA FFPE Kit (catalogue #80234) from Qiagen (UK).
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bioRxiv - Microbiology 2023Quote: ... Stigma tissues were removed from tube and DNA was extracted from the solution using DNeasy PowerSoil Pro Kit (Qiagen, Hilden Germany) following manufacturer’s instructions except the following modifications ...
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bioRxiv - Evolutionary Biology 2023Quote: ... edulis,DNA was extracted from gills of individual adults or from pool of larvae using the MagAttract® HMW DNA kit (Qiagen) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2023Quote: We extracted high molecular weight DNA from approximately 20 mg cortex tissue from each donor using the MagAttract HMW DNA kit (Qiagen 67563). We prepared linked read libraries using the Chromium Genome Reagent Kit v2 following the protocol provided by 10x Genomics ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was purified from soluble fraction under native conditions using Ni2+– NTA affinity chromatography (Ni2+ beads obtained from Qiagen, #30210), followed by a second step of purification involving gel filtration chromatography using Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Plant Biology 2023Quote: RNA was extracted from the division zone and different leaf samples from greenhouse-grown maize plants using RNeasy® Plant Mini Kit (Qiagen). Extracted RNA concentrations were quantified using a NanoDrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated from vaginal washes or from cultured bacteria using the DNeasy™ Blood & Tissue Kit (Qiagen, Netherland, Cat #69506). DNA was isolated as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant was aspirated from each well and the total RNA was then extracted from the cells using RNeasy mini kit (Qiagen, 74104). The total RNAs were quantified by ultraviolet absorption at 260 nm (Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was isolated from the Tr4 and Tr5 metamere progenitors dissected from 1hr APF pupae using RNeasy Micro Kit (QIAGEN, #74004). SMART-Seq v4 Ultra low input RNA Kit (Takara ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from approximately 30 mg of liver tissue from both infected and mock mice using the RNeasy Mini Kit (Qiagen, USA). Total RNA was then used to detect active infection by YFV (Domingo et al. ...
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bioRxiv - Microbiology 2024Quote: The total genomic DNA from the soil and rhizosphere was extracted from 250 mg of each sample using the DNeasy PowerSoil Kit (QIAGEN, Germany) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... DNA was then prepped from 96 colonies from each of the NlaIII- and BfaI-digested tumors using the Direct prep 96 miniprep kit (Qiagen #27361). Products were subsequently sequenced using the BigDye® Terminator v3.1 Cycle Sequencing Kit and ABI 3730xl DNA Analyzers (Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from freshly sorted 30,000 PDGFRα+ cells from LA/BC muscles using the RNeasy Plus Micro Kit (Qiagen, Cat# 74034) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: RNA was extracted from 7–9-week-old glial spheres (FMR1+/y and FMR1-/y) using the RNeasy micro kit from Qiagen (74004). RNA was converted to cDNA using RevertAid First Strand cDNA Synthesis Kit (K1621 Thermofisher) ...
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bioRxiv - Cell Biology 2020Quote: Genomic DNA was extracted from 2×105 primary human lung fibroblasts isolated from fresh and cryopreserved tissue from 3 different donors in technical replicates using QIAamp Micro Kit (Qiagen, Hilden, Germany) following manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... DNA from the white blood cells were extracted from 200 μl of buffy coat with the DNeasy Blood Tissue Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Parasite DNA was extracted from 200 ul from EDTA whole blood using EZ1 DNA blood kit with automated EZ1 Advanced XL purification system (Qiagen, Hilden, Germany). Real Time PCR for P ...
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bioRxiv - Plant Biology 2021Quote: ... The DNA amplicons derived from the bacterial 16S rRNA gene were extracted from the gels using the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany). After determination of the DNA concentration of each amplicon (nanodrop ...
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bioRxiv - Microbiology 2021Quote: ... Total RNAs from each cell line with different treatments were isolated by using the RNeasy Plus Mini kit from Qiagen (Cat#74134). The isolated RNAs were treated with RNase-free DNAse I at 37 °C for 30 min to remove genomic DNA ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from 0.25 g of soil from all segments from two of the five 13C soil cores using the DNeasy PowerSoil DNA Extraction kit (Qiagen, Hilden, Germany) to determine the segment with the highest abundance of methanogens or methanotrophs based on the respective functional marker genes using qPCR as described below ...
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bioRxiv - Cell Biology 2020Quote: ... 4 nmole standard desalted Ultramer™ DNA oligo from IDT) and PCR products (purified using QIAquick® PCR Purification Kit from QIAGEN) served as donor templates for small (3xFLAG epitope tag ...
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bioRxiv - Evolutionary Biology 2022Quote: ... High molecular weight DNA was extracted from 40mg frozen liver from both species using Qiagen Genomic Tip 100/G kit (Qiagen, Hilden, Germany). DNA integrity was assessed visually by separating fragments on a 0.5% TAE agarose gel which revealed a predominant band of high molecular weight DNA >45kb ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was resuspended in 30 μL of mQ water and incubated with 1 μL of RNase (10 mg/mL prepared from a stock of 100 mg/mL of RNase A from Qiagen, Darmstadt, Germany) at 37 °C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted and purified from 20 mg (2 pellets) of stool from each mouse using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Germantown, MD). The concentration of DNA in samples was determined by spectrophotometry ...
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bioRxiv - Evolutionary Biology 2022Quote: ... High molecular weight DNA was extracted from cell pellets of overnight cultures following the protocol for gram negative bacteria from the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). Long-read sequencing was performed at the Norwegian Sequencing Centre according to the following protocol ...