Labshake search
Citations for Qiagen :
501 - 550 of 10000+ citations for Fumarase from porcine heart since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... total RNA from the hypothalamus and the hippocampus isolated from five Tshr+/+ mice was extracted using an RNeasy Mini kit (Qiagen) per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was extracted from frozen tissue using the QiaAmp Mini Kit or the DNA/RNA extraction mini kit from Qiagen. DNA was quantified and quality checked with the Qubit fluorometer or the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was isolated from selected individuals from both liver and muscle using DNeasy blood and tissue kits (Qiagen, Hilden, Germany). Primers were designed to specifically amplify the regions around the CRISPR gRNA target sites (Table 1) ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA from cultured cells was isolated with Direct-zol RNA miniprep (Zymogen) and from tissues using miRNeasy Mini kit (Qiagen) according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2020Quote: ... Extraction of genomic DNA (gDNA) from 200µL of whole blood or from tissues using the Gentra Puregene kit and Tissue Lyser II (Qiagen, France) was performed accordingly to manufacturer recommendations.
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bioRxiv - Evolutionary Biology 2021Quote: ... Most DNA samples for ONT sequencing were extracted from 100 female flies from each strain using the Blood and Cell Culture DNA Mini Kit (Qiagen) following manufacturer’s instructions with small modifications (Table S2 ...
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bioRxiv - Physiology 2020Quote: ... The supplementary protocol for “Purification of archive-quality DNA from nematode suspensions using the Gentra Puregene Tissue Kit” available from Qiagen was used to isolate DNA ...
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bioRxiv - Microbiology 2021Quote: ... total environmental DNA was extracted from approximately 5 g of sediment subsampled from inner part of WRC using DNeasy PowerMax Soil Kit (Qiagen) with a minor modification 79 ...
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bioRxiv - Genomics 2021Quote: ... DNA from six larvae from each of the five breeding sites was extracted either with the QiaAMP UCP DNA kit (Qiagen) or MagAttract HMW DNA extraction kit (Qiagen) ...
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bioRxiv - Cancer Biology 2021Quote: RNA extraction from MNC cells from CCS or healthy donor was performed using the RNeasy Mini Kit (Qiagen, Milan, Italy) and quantified using a NanoDrop spectrophotometer (Nanodrop Technologies ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was purified from Achilles enthesis of 1-dpi pups or from tissue culture cells using the RNeasy Kit (Qiagen). Reverse transcription was performed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA was extracted from the sorted cells from different conditions using a RNeasy Mini Kit (Cat. No. 74106, QIAGEN). The total RNA was reverse transcribed into cDNA using iScript™ Reverse Transcription Supermix (Cat ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was extracted from Drosophila heads from three independent crosses for each genotype with the RNeasy Mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Total RNA from 700,000 to 1.25 million RGCs from 4 independent rat litters per developmental stage was isolated using the Qiagen RNAeasy kit from Qiagen, which included a 15 minutes On-column DNAse step ...
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bioRxiv - Genomics 2021Quote: We collected high molecular weight genomic DNA from whole blood or tissue from 36 coyotes sampled from Louisiana and 10 red wolves from North Carolina using the DNeasy Blood and Tissue Kit (Qiagen) and followed the manufacturer’s protocol for mammals ...
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bioRxiv - Biophysics 2021Quote: ... Capture probes that contained locked nucleic acid (LNA) residues were purchased either from IDT with a 5′ Cy3 modification and HPLC purification or from Qiagen with a 5′ amino modification with HPLC purification ...
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bioRxiv - Immunology 2021Quote: ... DNA was extracted from PBMCs obtained from diverse blood withdrawals (Supplementary Fig. S1) and MLung-derived TILs with DNeasy Blood & Tissue Kit (Qiagen). Whole exome sequencing and single nucleotide variant analyses from MInt were available from our previous publication and were afterwards obtained for MLung ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from freshly isolated T-cells on day 7 of treatment from spleens using RNeasy Kit (Qiagen). For each group ...
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bioRxiv - Immunology 2021Quote: Viral RNA was isolated from rhesus macaque plasma from 2 weeks post-SIV infection using an Ultrasense Viral RNA kit (QIAGEN) and cDNA was reverse transcribed using the Applied Biosystems High Capacity cDNA synthesis kit (Thermo-Fisher) ...
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bioRxiv - Immunology 2020Quote: ... 9,000-45,000 CD45+ islets infiltrates were sorted from KO vs WT BMT recipients from WT NOD and cell pellets were lysed in 33% RLT lysis buffer from QIAGEN and up to 3.5μl lysate was used directly in a 15μl hybridization reaction which was kept at 65°C for 24 hrs ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was isolated from a suspension of 1 x 106 PBMCs from each individual in the study using a DNA isolation kit (Qiagen). Genotyping was then performed using the Infinium CoreExome-24 (v1.3 ...
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bioRxiv - Genomics 2021Quote: Total RNA was isolated from ∼30 mg of gonadal tissue from each individual using the RNeasy Mini kit (Qiagen, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... RNA was isolated from chondrocytes using the RNeasy Mini Kit and from connective tissue using the RNeasy Fibrous Tissue Mini Kit (Qiagen). All other tissues were first homogenized using Lysing Matrix S ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products with sizes from 150 bp to 300 bp were extracted from 2% agarose gel using MinElute Gel Extraction Kit (Qiagen).
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bioRxiv - Microbiology 2023Quote: During this time a stock of genomic DNA (gDNA) from Escherichia coli (REL606) from ∼72 mL of overnight culture was made using the “DNeasy Ultraclean Microbial kit” (Qiagen), resulting in a stock of ∼30 mL of gDNA at 20 μg/mL (stored at -20 °C) ...
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bioRxiv - Developmental Biology 2023Quote: ... total RNA was purified from gonad-mesonephos complexes at E11.5 and from isolated gonads at E12.5 using the RNeasy Micro Kit (Qiagen, Hilden, Germany). For each genotype ...
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bioRxiv - Cell Biology 2023Quote: ... The total RNA was isolated from each slice separately using an adapted protocol from RNeasy Mini Kit (RNeasy Mini Kit #74104, Qiagen). Each slice was placed into a 2ml low binding tube (DNA LoBind® #0030108078 ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from FACS-sorted L-GMPs from BL/6 and Il21-/- AML mice using the RNeasy Micro Kit (Qiagen). RNA purity was checked using the NanoPhotometer® spectrophotometer (Implen) ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA isolation from buccal swab samples: Genomic DNA was isolated from the buccal swab samples using a DNeasy Blood/Tissue Kit (Qiagen) according to the standard protocol ...
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bioRxiv - Cell Biology 2024Quote: ... DNA fragments ranging from 350 to 700 bp were excised from the gel and purified using a QIAGEN gel extraction kit (QIAGEN). PCR and library preparation were conducted as previously described25 ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA from 7 of these patients was extracted from CD138+ cells using the miRNeasy Mini Kit (Qiagen GmbH, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from FACS-sorted B220low/CD19+ splenocytes from sick mice (B-cell lymphoma only) using an RNeasy Micro kit (Qiagen). Control RNA was isolated from normal B220high/CD19+ splenocytes from control healthy mice that had also received pIpC and SRBC injections similarly to the experimental mice ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from FACS-sorted B220low/CD19+ splenocytes from sick mice (B-cell lymphoma only) using a RNeasy Micro kit (Qiagen). Control RNA was isolated from normal B220high/CD19+ splenocytes from control healthy mice that also received pIpC and SRBC injections similarly to the experimental mice ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was extracted from mixed stage growing populations using reagents from the Puregene Core Kit A (QIAGEN, Valencia, CA) and quantified using Qubit 3.0 fluorometer (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: DNA was extracted from fresh frozen lung cancer tissue embedded into OCT (TissueTek, Sakura) and from PBMCs isolated from preserved patient-matched blood (AllPrep DNA/RNA extraction kit, Qiagen). Library construction was performed as previously described106 ...
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bioRxiv - Evolutionary Biology 2023Quote: We extracted total RNA from liver and BAT from each sample (n = ∼6 per genotype/sex/treatment/tissue) using the RNeasy PowerLyzer Kit (QIAGEN). We generated Illumina cDNA libraries from 1 μg of purified RNA using KAPA Stranded mRNA-Seq Kit (Illumina) ...
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bioRxiv - Physiology 2023Quote: ... and LysoSensor Green DND-189 (cat. L7535) were purchased from Thermo. MinElute PCR Purification Kit (cat. 28004) were purchased from Qiagen. Biospin Tissue Genomic DNA extraction Kit (cat ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from mouse brains with TRIzol or from selected CD11b or CD4-positive cells with RNeasy Plus Mini Kit (Qiagen) and reverse-transcribed ...
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bioRxiv - Neuroscience 2023Quote: Total RNA from the frozen ipsilateral side of the spinal cord was extracted using RNeasy Plus Universal kit from Qiagen and quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2023Quote: ... RNA from viruses was extracted from egg allantoic fluid and cell culture medium by using the QIAamp viral RNA minikit (Qiagen). A Verso cDNA synthesis kit (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... A DNA fragment containing 248nt of the NF1 coding region of interest was PCR-amplified from single-strand cDNA obtained from parental and clonal cells using QIAquick Gel Extraction Kit (QIAGEN). Incorporation was confirmed by Sanger sequencing the amplicon ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA was isolated from whole blood (or in 19 cases directly from the fibroblasts) using DNEasy Blood & Tissue Kit (Qiagen), quantified ...
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bioRxiv - Molecular Biology 2023Quote: Cells collected from either 3D culture organoids or sorted cells were lysed in RLT buffer from RNeasy kit (Qiagen, 74106). RNA samples were isolated using the same kit following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA samples were obtained from dissected tissues from adults or from a pool of embryos or larvae using RNAble solution (Eurobio) and a TissueLyser II system (Qiagen). Total RNA samples were treated with DNAse I (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... grown up at 20 °C were washed off from NGM plates using M9 solution and subjected to total RNA extraction using the RNeasy Mini Kit from Qiagen. Purification of poly-A containing RNA molecules ...
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bioRxiv - Cancer Biology 2022Quote: DNA and RNA from tumor specimens and DNA from matched blood samples were isolated using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen). For formalin-fixed and paraffin-embedded (FFPE ...
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bioRxiv - Cell Biology 2022Quote: ... animals were maintained at 20 °C and washed down from NGM plates using M9 solution and subjected to RNA extraction using TissueDisruptor and the RNeasy Mini Kit from Qiagen. RNA preparations were used for qRT-PCR or RNAseq ...
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bioRxiv - Developmental Biology 2022Quote: ... the animals were washed down from NGM plates using M9 solution and subjected to RNA extraction using the RNeasy Mini Kit from Qiagen. 1 μg total RNA from each sample was used for sequencing library construction ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA and RNA were extracted from ten 30-μm sections from each tumor using the QIAmp DNA FFPE Tissue Kit (Qiagen) and miRNeasy Kit (Qiagen ...
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bioRxiv - Genomics 2022Quote: ... The DNA from mouse bladder tumors and matched germline DNA from mouse tails were extracted using the DNeasy Blood Tissue kit (Qiagen) according to the manufacturer’s instructions ...