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Citations for Qiagen :
701 - 750 of 887 citations for 26S Proteasome Non ATPase Regulatory Subunit 10 PSMD10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... colonic Foxp3(GFP)+IL-10(Thy1.1)+ and Foxp3(GFP)+IL-10(Thy1.1)- cells were sorted into Trizol and total RNA was isolated with miRNeasy Micro Kits (QIAGEN 217084) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The bead-bound gDNA was isothermally amplified for 3 hours at 30 °C then 10 minutes at 65 °C using a miniaturised (1/5 vols) Repli-g Single-Cell assay (Qiagen). The amplified gDNA was cleaned up with 0.8 × vols Ampure XP and 80 % ethanol ...
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bioRxiv - Biochemistry 2022Quote: ... complexes were either concentrated using a 50 kDa MWCO concentrator and further purified on a Superose 6 Increase 10/300 column (Cytiva) equilibrated in low-salt CSFxB or loaded directly onto Ni2+ agarose (Qiagen), eluted using 300 mM imidazole ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and 1x antibiotic-antimycotic by a TissueLyser II bead mill with 5-mm stainless steel beads (Qiagen). After brief centrifugation ...
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bioRxiv - Genomics 2023Quote: ... Samples were clarified by centrifugation at 2,000 rpm for 10 minutes and subjected to nucleic acid extraction using the cador Pathogen 96 QIAcube HT Kit (Qiagen) in a QIAcube HT automated extractor (Qiagen).
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bioRxiv - Cancer Biology 2023Quote: ... Following complete removal and evaporation of residual ethanol (10 mins at 30°C) DNA was extracted using the AllPrep DNA/RNA FFPE Kit (Qiagen). DNA was eluted in 40 µl Elution buffer.
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bioRxiv - Biochemistry 2023Quote: ... The pellet was then air dried for 1 minute and re-suspended in 50 μL Buffer EB (10 mM Tris-Cl, pH 8.5; All buffers from QIAprep Spin Miniprep Kit, Qiagen #27104). Purified bacmid was then transfected into Sf9 cells ...
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bioRxiv - Microbiology 2023Quote: RNA was isolated from 2 h and 10 h RPMI-grown and macrophage-internalized Cg cells using the RNeasy kit (Qiagen), followed by DNase I digestion ...
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bioRxiv - Microbiology 2023Quote: ... 2 ml of the culture was harvested and centrifuged at 11,000 × g for 10 minutes where the cell pellet was used for genome extraction with the DNeasy Blood & Tissue Kit (Qiagen) protocols ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from five 10-mL aliquots of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Microbiology 2023Quote: ... Metagenomic sequencing libraries were constructed from extracted DNA samples with 10 μL (1/5 volume) reactions using the QIAseq FX DNA Library Kit (QIAGEN). Each metagenomic sequencing library was sequenced using the Illumina NextSeq 2000 System 2 x 150 bp configuration.
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bioRxiv - Microbiology 2023Quote: ... BALF samples and 10% tissue homogenates were mixed with an equal volume of the RLT buffer (Qiagen, Germantown, MD, USA).
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bioRxiv - Microbiology 2023Quote: ... Pellets were washed in 2 mL cold 10 mM NaCl + 4 mL cold RNAprotect Bacteria Reagent (Qiagen Cat. No. 76506) and repelletted at 4,255 x g ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from ∼10–60mg of muscle tissue was extracted using a Qiagen miRNeasy Mini kit (217004, Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... the FT and Eluate fractions (90 µL each) were mixed with 10 µL 3 M sodium acetate and applied to a QIAquick spin column (Qiagen). Purified DNA was visualized a 1.3% agarose / 0.5x TBE gel and SYBR Green staining ...
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bioRxiv - Biochemistry 2023Quote: ... Glycerol was added to the supernatant to a final concentration of 10 % and was then incubated with Ni-NTA resin (QIAGEN) for two hours ...
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bioRxiv - Neuroscience 2023Quote: ... 3 μL were used as template in a 10 μL final volume reaction using the miRCURY LNA SYBR Green PCR Kit (Qiagen) in a StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from roots of a 14-day-old Col and GFP-GrxS5 plants grown on 10 mM NO3- using RNeasy Mini Kit (QIAGEN). We used 1 μg total RNA for mRNA purification using the NEBNext Oligo d (T ...
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bioRxiv - Developmental Biology 2023Quote: ... Then samples and inputs were incubated for 4 hr at 37°C with 1.5 µl of 10 mg/ml RNase A (Qiagen, 1007885) and 15 µl 10% sodium dodecyl sulfate and 3.5 µl 20 mg/ml Proteinase K (Thermo Fisher ...
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bioRxiv - Genetics 2023Quote: Total RNA was isolated from 10 third-instar larvae for each biological replicate (3) using an RNeasy Lipid Tissue Mini kit (Qiagen). DNA contamination was removed using TURBO DNA-free (Ambion) ...
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bioRxiv - Immunology 2023Quote: ... or 10 ul buffy coat (from Accio Biobank or NHS) using the DNeasy Blood and Tissue Kit (Cat# 69504, Qiagen) following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: ... while samples for RNA-based analyses were mixed at a 1:10 ratio of LifeGuard Soil Preservation (Qiagen, Hilden Germany) before being put on dry ice ...
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bioRxiv - Biochemistry 2023Quote: ... SUMO protease was added at a final concentration 1/10 and the mixture was dialyzed overnight at 4 °C against the buffer DB6 (50 mM Tris-HCl pH 8, 150 mM NaCl, 10 mM imidazole) and applied on a NiNTA column (Qiagen) equilibrated in the DB6 buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted from the supernatant (12,000 x g for 10 min at 4°C) of centrifuged tissue homogenate using an RNeasy Mini Kit (Qiagen, USA). The extracted total RNA was used for single-strand cDNA synthesis ...
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bioRxiv - Genetics 2024Quote: Total genomic DNA was extracted from 10% suspension of fin clips (<1 mm2) using the DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer’s protocols ...
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bioRxiv - Genetics 2024Quote: ... qRT-PCR was performed in a 10 μL volume using the miRCURY LNA SYBR Green PCR Kit (Qiagen, Hilden, Germany) and the following miRCURY LNA miRNA PCR primer sets (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were used at the following dilutions: 1:1000 anti-penta-His mouse monoclonal (Qiagen), 1:5000 anti-cMyc mouse monoclonal (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-His Western blots were performed using Penta-His mouse monoclonal IgG1 as primary antibody (Qiagen, #34660) at 1:2000 dilution ...
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bioRxiv - Microbiology 2019Quote: ... and Western blot analysis was performed as previously described (30) using a primary anti-his antibody (Qiagen) and a secondary Alexa Fluor 700 goat anti-mouse antibody ...
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bioRxiv - Microbiology 2021Quote: ... plasmids bearing chimeric antibody heavy and light chain genes were prepared (EndoFree Plasmid Mega prep Kit, QIAGEN) and transfected into Human Embryonic Kidney cells (HEK293F ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein bands were transferred to a nitrocellulose membrane and probed with primary antibodies [mouse anti-His (Qiagen) to detect CAT ...
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bioRxiv - Microbiology 2023Quote: ... Proteins transferred to a nitrocellulose membrane were probed with a monoclonal anti-His6 antibody (Qiagen, Germantown, Maryland). Detection of proteins via Western blotting was performed by fluorescence detection using IR-Dye®-labeled fluorescent secondary antibodies and imaged using the Odyssey CLx Imager (LICOR Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... The purified recombinant proteins were analyzed by western-blot with anti-His antibody (1:2000 dilution) (Qiagen) followed by HRP-labeled anti-mouse IgG (1:50000 dilution ...
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bioRxiv - Immunology 2023Quote: ... Plasmids with the cloning of synthesized antibody genes were prepared using the Megaprep plasmid plus kit (Qiagen) for transient transfection ...
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bioRxiv - Zoology 2021Quote: ... PCR amplifications were carried out in 10 μl reaction volumes containing 5 μl Type-it Microsatellite PCR Kit (Qiagen, Hilden, Germany), 0.5 μM of each primer ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted and 5 μg reverse transcribed from paired isolated Jz and Lz placental tissues (n = 8-10 per genotype/sex, across 11 litters) using the RNeasy Plus Mini Kit (Qiagen, DE) and the High-Capacity cDNA Reverse Transcription Kit minus RT inhibitor (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Multiplex amplification was carried out in 10 μl reaction volumes containing 5 μl of QIAGEN Multiplex PCR Buffer Mix (QIAGEN Inc.), 0.2 μM labeled forward primer (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total RNA was extracted from 10 samples (5♀♀, 5♂♂) using RNeasy Micro kits (QIAGEN K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.5 µl of diluted template cDNA (1:3, nuclease-free water) per real-time PCR reaction (10 µl – SYBR Green PCR kit, Qiagen, 204143) was used to assay specific transcript abundance (CFX96 Real-Time System ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA (gDNA) was extracted from snap-frozen tissues (∼10–20 mg) using DNeasy Blood and Tissue Kit (Qiagen, Cat# 69504) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was prepared from HT29 cells treated with 10 mM BG for 48 h utilizing RNeasy Mini kit (Qiagen, Germany), and was subjected to microarray and RNA sequencing analyses as described previously (Sasaki et al. ...
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bioRxiv - Immunology 2020Quote: Naso- and oropharyngeal swab samples were centrifuged at 17,000xg for 10 mins at 4°C to obtain cell pellets which were washed once using PBS and lysed by bead-vortexing for 10 minutes in cell lysis buffer (RLT, Qiagen, Germany). Proteins (as well as DNA and RNA ...
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bioRxiv - Immunology 2020Quote: Airway samples were centrifuged at 17,000xg for 10 mins at 4°C to obtain cell pellets which were washed once using PBS and lysed by bead-vortexing for 10 minutes in cell lysis buffer (RLT, Qiagen, Germany). Proteins (as well as DNA and RNA ...
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bioRxiv - Biochemistry 2020Quote: ... was mixed with selection plasmid pCoBlast (1:10) and the mixture was used for cells transfection by Effectene Transfection Reagent (QIAGEN, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... total RNA from the fibroblasts cultured with 10 μM Dwn1 was extracted every 6 hours from hours 24 to 48 (RNeasy® Plus Mini kit, Qiagen), followed by cDNA synthesis (SuperScript® VILO™ cDNA Synthesis Kit ...
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bioRxiv - Microbiology 2020Quote: ... Each 10 μL uPCR reaction contained 2 μL of DNA template with 1x QuantiTect Multiplex PCR No ROX mastermix (Qiagen™), 0.4 μM each primer ...
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bioRxiv - Neuroscience 2020Quote: DNA and RNA from 8-10 mice was isolated and purified with AllPrep-DNA/RNA/miRNA-universal kit (Qiagen, Montreal, QC) and concentrations were determined by fluorometry on the Qubit system (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: Cells debris was pelleted by centrifugation at 23,000 x g for 45 minutes and the supernatant loaded onto a 10 ml Ni2+-NTA column (Qiagen, Hilden, Germany). The column was washed with 600 ml buffer A and protein batch eluted with 100 ml buffer B (buffer B = buffer A supplemented with 290 mM imidazole).
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bioRxiv - Neuroscience 2020Quote: ... The high-quality reads were de novo assembled using the short reads assembly program Trinity version 2.3.2ref56 as well as a CLC Genomics Workbench version 10 (Qiagen, Carlsbad, CA, USA). The Trinity assembly yielded a total of 74,151 predicted ‘genes’ with their isoforms totaling 171,567 predicted ‘transcripts’ (i.e. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 10-20 mg of silica-dried stem or leaf tissue was ground with a Qiagen TissueLyser II (Qiagen, Melbourne, Vic., Australia). DNA was extracted using the DNeasy 96 Plant Kit or DNeasyPlant Mini Kit (Qiagen ...