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Citations for Qiagen :
551 - 600 of 887 citations for 26S Proteasome Non ATPase Regulatory Subunit 10 PSMD10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Each sample was normalized to contain 10 ng/μl DNA using QIAgility Liquid Handling System (Qiagen, Germantown, Maryland, USA) for library construction ...
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bioRxiv - Cell Biology 2021Quote: RNA from 5-10 million NK cells treated overnight with indicated conditions was extracted using Rneasy Plus kit (Qiagen). Samples were prepared for Illumina sequencing following the manufacturer’s protocol using the TruSeq® stranded total RNA with rRNA depletion using Ribo Zero TM Human/Mouse/Rat ...
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bioRxiv - Cell Biology 2021Quote: Tissue lysates were further homogenized and centrifuged at 10,000 g for 10 min at 4°C in microcentrifuge spin columns (QIAshredder, Qiagen) to obtain clear protein lysate ...
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bioRxiv - Microbiology 2021Quote: ... The centrifugation step was repeated and the pellet air-dried before the DNA was dissolved in 10 mM Tris-HCl (pH 8.5, Qiagen) at 65 °C for 20 min ...
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bioRxiv - Systems Biology 2020Quote: ... The sealed plates were subjected to 10 minutes of bead beating at 20 Hz using an (Qiagen Tissuelyser II). After bead beating ...
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bioRxiv - Microbiology 2019Quote: ... Samples were centrifuged at 14,000 × g for 10 min and RNA was extracted using Qiagen RNeasy mini plus kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Crystals of Brd4(BD1) with YFV-C (1-10) K4ac peptide were grown from a solution containing 0.2 M sodium chloride (QIAGEN), 0.1 M Tris (pH 7.0 ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from platelet pellets (10 million per population) using the RNeasy Mini kit according to manufacturer’s instructions (Qiagen). cDNA was synthesized from extracted platelet RNA using SuperScript® III first-strand synthesis system for RT-PCR (Life Technologies) ...
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bioRxiv - Biochemistry 2022Quote: ... 10% glycerol and 1 mM DTT) and recombinant TrcP was purified from clarified lysates using Ni-NTA resin (Qiagen) and gravity chromatography ...
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bioRxiv - Molecular Biology 2022Quote: His-Prs or its variants (10 µg) were bound to 4 µl of Ni-NTA Magnetic Agarose Beads (Qiagen) in 50 µl interaction buffer (50 mM Tris pH 9.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... Embryos and the surrounding maternal calyptra tissue were dissected out of the archegoniophore into 10% RNALater (Qiagen, Hilden, Germany) on Microscope slides with cavities (Marienfeld Superior ...
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bioRxiv - Plant Biology 2022Quote: ... Sections were immediately placed into 96-well plates (on ice) containing 10 μL of RLT plus (Qiagen, Hilden, Germany). All instruments and surfaces were cleaned with 80% ethanol ...
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Variation in Leishmania chemokine suppression driven by diversification of the GP63 virulence factorbioRxiv - Microbiology 2021Quote: ... parasites were spun at 1300g for 10 minutes and resuspended in buffer RLT from the RNeasy Mini Kit (Qiagen). Genomic DNA was removed from the sample using TurboDNase (Thermo ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from the sediment samples using the larger format PowerMax Soil DNA Isolation Kit (12988-10, QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was discarded and 3.5 ml of RLT buffer with B-mercaptoethanol (10 µl for every 350 µl of RLT buffer – Qiagen) was added ...
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bioRxiv - Physiology 2022Quote: 10 mg of liver were used to extract DNA with the DNeasy Blood & Tissue Kit (QIAGEN, cat. no. 69506), according to the manufactureŕs instructions ...
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bioRxiv - Bioengineering 2020Quote: ... each SeraCare panel member was diluted 1:10 in human plasma to a final volume of 100μL and processed by QIAGEN QIAamp viral mini kit ...
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bioRxiv - Molecular Biology 2021Quote: Total genomic DNA was extracted from 10 coastal plant species (Table S2) using the Plant DNeasy Mini Kit (QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... SYBR green RT-qPCR was performed in triplicate with 10 ng of template cDNA using QuantiTect Master Mix (Qiagen) on a 7900-HT Fast Real-time System (Applied Biosystems ...
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bioRxiv - Developmental Biology 2020Quote: ... the allantois was dissected out and lysed in 10 μl DirectPCR (Tail) digest reagent (Qiagen, catalog no. 102-T) supplemented with 1 μl of proteinase K overnight at 55°C ...
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bioRxiv - Genomics 2022Quote: ... 10 budgerigar samples and 12 zebra finch samples) using the DNeasy® Blood & Tissue Kit (Cat. No. 69581, Qiagen). For PCR ...
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bioRxiv - Microbiology 2020Quote: DNA for metagenomic sequencing was extracted from ~7 g sediment (~0.7 g sediment in 10 individual lysis tubes) using PowerSoil DNA Isolation Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... We eluted purified DNA in 45 µl of EB buffer (10 mM tris-hydrochloride (pH 8.0) (Qiagen, The Netherlands) supplemented with 0.05% (v/v ...
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bioRxiv - Immunology 2021Quote: ... Second-strand synthesis reaction was carried out in 100 μl using 10 pmol of each primer (HotStarTaq Plus, Qiagen). Residual primers and dNTPs were again degraded enzymatically (ExoSAP-IT ...
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bioRxiv - Immunology 2021Quote: ... or ectodomain buffer (20 mM Tris, pH 8.5, 500 mM NaCl) and applied to 10 ml Ni-NTA-Sepharose (Qiagen) columns ...
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bioRxiv - Microbiology 2021Quote: ... Samples were spun at 5000 x g for 10 minutes and supernatant was discarded before resuspension in 1.5 mL QIAzol reagent (Qiagen). Screw-cap tubes were prepared for each sample by adding ∼250 µL of sterile beads and the QIAzol suspension mixture was added and incubated for 5 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was lysed with a lysis buffer (10 mM Tris-HCl, pH 8.0, 150 mM NaCl, 20 mM EDTA, 1% SDS) and proteinase K (Qiagen) was added to samples to degrade proteins ...
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bioRxiv - Systems Biology 2022Quote: ... the bacteria cells were scraped and centrifuged at 3,000g for 10 min and the plasmids were then extracted (Qiagen). Cloning of individual sgRNA base editor construct was based on similar procedure with reduced reagent input.
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was loaded onto a 10 mL Ni-NTA Superflow cartridge (connected two 5 mL cartridges) (30761, Qiagen). The cartridge was washed with 100 mL R buffer containing 200 mM NaCl ...
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bioRxiv - Genetics 2023Quote: ... leaf tissues were collected and homogenized in 400 μL of TPS buffer (100 mM Tris-HCl, 10 mM EDTA, 1 M KCl, pH8.0) using TissueLyser II (Qiagen), followed by incubation for 20 min at 75°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Sporophytes and the surrounding maternal calyptra tissue were dissected out of the archegoniophores into 10% RNALater (Qiagen, Hilden, Germany) on Microscope slides with cavities (Marienfeld Superior ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated with Angiotensin-II (1 μM) alone or in combination with DAPT (10 μM) or siNotch1 (Qiagen) (50 nM ...
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bioRxiv - Neuroscience 2023Quote: Sixty 10 μm cryosections of quadriceps muscle snap frozen in liquid nitrogen were collected in QIAzole (Qiagen Cat#79306). Four male and four female wildtype and Col6a2-/- mice were included at two different age groups (5 weeks and 25 weeks old) ...
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bioRxiv - Cancer Biology 2022Quote: ... after adding 1.0×10^6 copies/µL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Colonic organoids were stimulated with 10% cCM or iCM for 24h prior to lysis and RNA isolation (Qiagen RNeasy). Quadruplicates were sequenced.
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bioRxiv - Cell Biology 2023Quote: ... Overnight cultures were pelleted at 3,000g for 10 min and plasmid DNA was purified using a Qiagen miniprep kit (27106, Qiagen). Sequences were verified by Sanger sequencing (Eton Bioscience Inc).
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bioRxiv - Systems Biology 2023Quote: ... x mg solid matrix were mixed with five times the μl amount of ACN:water (1:1, v/v) and homogenised with a TissueLyser II (30 Hz, 10 min; Retsch Qiagen). After a short centrifugation (2 min ...
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bioRxiv - Microbiology 2023Quote: ... SYBR green real-time PCR assay was carried out in a 20μL PCR mixture volume consisting of 10 μL of 2X Quantitect SYBR green RT-PCR Master Mix (Qiagen) containing HotStarTaq DNA polymerase ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated in lysis buffer (DPBS with 0.5% Triton X-100, 10 mM MgCl2, 5 mM CaCl2, 100 µg/mL RNAse A (Qiagen)) overnight at 37°C in a water bath to allow capsid maturation ...
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bioRxiv - Microbiology 2023Quote: ... for 10 minutes at 37°C followed by a final clean-up with Qiagen RNeasy MinElute Kit (Qiagen, #74204) using default manufacturer’s protocol (which keeps RNAs > 200nt ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µg⋅ml –1 Leupeptin and 3 mM Benzamidine) with steel beads at 28.5 Hz for 1 min (Qiagen TissueLyser II ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from 5-10×106 cells using the Gentra Puregene Cell Kit (Qiagen, cat no. 158046) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... doxycycline was added at (0.1 μg/ml, 1 μg/ml (CHRDL2 +) or 10 μg/ml (CHRDL2 ++) RNA was extracted (RNeasy, QIAGEN) and quantified by real-time reverse transcriptase polymerase chain reaction (qPCR ...
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bioRxiv - Cancer Biology 2023Quote: ... and 9-10 mL of Buffer BB (1/3 of the lysate volume, From QIAGEN Plasmid Plus Midi Kit) was added ...
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bioRxiv - Genomics 2023Quote: ... Plasmid transfections were performed in six-well tissue-culture treated dishes at 1.8 × 10^6 cells/ml using Effectene (301427; QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The lysate was centrifuged at 50,000×g for 10 min and to the supernatant 1 mL of 50% Ni−NTA (Qiagen) was added and incubated for 1 h at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA quality was estimated through total RNA extraction from 10 tissue sections with a RNeasy Plus Mini kit (Qiagen). Thereafter RNA integrity number (RIN ...
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bioRxiv - Molecular Biology 2024Quote: ... QT65005) and 10 µM each of forward and reverse primers (listed in Table S2) on Rotor-Gene Q (QIAGEN) with PCR procedures under the following programme ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from approximately 5-10 million cells from each organ using the RNeasy Mini Kit (QIAGEN). SuperScript® IV Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... the proteins were transferred to nitrocellulose membranes before being revealed with anti-Histidine monoclonal antibodies (Qiagen). Immune complexes were detected with anti-rabbit peroxidase-conjugated secondary antibodies (Promega ...