Labshake search
Citations for Qiagen :
651 - 700 of 1868 citations for Dengue Virus Serotype 3 Envelope Protein Mouse Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: RNA was extracted from six hundred testes of ≤3 days old using miRNeasy (QIAGEN). Less than 200 nt fragments were collected using miRelute column (QIAGEN) ...
-
bioRxiv - Genetics 2023Quote: ... and a single 3 mm metal bead (Eldan, BL6693003000) using TissueLyzer LT (QIAGEN, #85600) with a dedicated adaptor (QIAGEN ...
-
bioRxiv - Microbiology 2023Quote: ... for 3 minutes at a 30Hz/s frequency in a TissueLyser II grinder (Qiagen). Vero cells were seeded in 24-well plates and incubated for 24 hours to reach confluency ...
-
bioRxiv - Cell Biology 2023Quote: ... pools of 3 x 103 MPP1-4 were sorted directly into RLT buffer (Qiagen) enriched with 1% of β-mercaptoethanol ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were sorted into 384 well plates each containing 3 μl Vapor-Lock (Qiagen) in all wells and eight different conditions of 0.3 μl lysis buffer consisting of 0.125μM OligodT30VN (5’-Biotin-ACGAGCATCAGCAGCATACGAT30VN-3’ ...
-
bioRxiv - Immunology 2020Quote: ... Mouse and Human IFN I RT2 Profiler PCR Arrays (Qiagen) were performed and relative expression determined using the ∆∆CT method and normalized for 5 housekeeping genes according to manufacturer’s guidance.
-
bioRxiv - Cancer Biology 2019Quote: ... cells were transfected with AllStars Mouse Negative Control siRNA (Qiagen) or siKras (siKRAS_234 as described by Yuan et al.59 ...
-
bioRxiv - Developmental Biology 2021Quote: ... Total mouse RNA was isolated using RNeasy mini kit (Qiagen) and reverse transcribed to cDNA using an oligo (dT ...
-
bioRxiv - Cell Biology 2021Quote: ... Frozen mouse liver were first homogenized using TissueLyser II (Qiagen) and then lysed in 1X Laemmli buffer ...
-
bioRxiv - Immunology 2022Quote: ... or from frozen mouse BMDMs using RNeasy Mini kit (Qiagen). Reverse transcription (RT ...
-
bioRxiv - Developmental Biology 2023Quote: ... RT² Profiler™ PCR Array Mouse Neurogenesis (330231, Qiagen, Germany) was performed according to the manufacturer’s instructions on LightCycler 96 (Roche ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... We used Penta-His mouse monoclonal antibody (Qiagen, Qiagen #34660) at a 1:2000 dilution in 5% BSA in PBS-T buffer for 1 h ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... We used Penta-His mouse monoclonal antibody (Qiagen, Qiagen #34660) at a 1:2000 dilution in 5% BSA in PBS-T buffer for 1 h ...
-
bioRxiv - Physiology 2023Quote: ... RT2 Profiler PCR Array for Mouse Fibrosis (PAMM-120Z, Qiagen) and Qiagen GeneGlobe Analysis was used to evaluate fibrosis between normal saline and experimental groups at each time point.
-
bioRxiv - Microbiology 2023Quote: ... blots were incubated with mouse monoclonal RGS-His antibody (Qiagen) diluted 1:1,000 ...
-
bioRxiv - Immunology 2021Quote: Total RNA was isolated from 3×106 neutrophils by using the RNeasy Mini Kit (Qiagen). TaqMan real-time quantitative RT-PCR was performed utilizing the Applied Biosystems StepOne Plus cycler (Applied Biosystems ...
-
bioRxiv - Systems Biology 2020Quote: ... with 3 mm beads then extracted according to the protocol using RNeasy Mini Kit (Qiagen).
-
bioRxiv - Microbiology 2020Quote: ... vIL-6 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) using QuantiTect Multiplex RT-PCR Kits (Qiagen) as described previously (37 ...
-
bioRxiv - Microbiology 2021Quote: ... 3 × 105 cells were harvested and total RNA was extracted using the RNeasy kit (Qiagen) employing on-column DNase treatment ...
-
The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... crassa strain 87-3 (bd+, mat a) using the Gentra Puregene Tissue kit (Qiagen, 158622) and following the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2022Quote: ... and roots of the cultivar Hoko-3 using RNeasy Plant Mini kit (Qiagen, Hilden, Germany). Library preparation was performed using NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs ...
-
bioRxiv - Microbiology 2023Quote: ... Typhimurium strain were (n = 3) were extracted using the Rneasy kit (Qiagen Sciences, Maryland, USA), after an overnight culture in CBD tinted LB broth (for CBD-resistant strain ...
-
bioRxiv - Plant Biology 2023Quote: ... Two glass beads were added before shaking for 3 minutes using a Tissue Lyser (QIAGEN) at maximum speed ...
-
bioRxiv - Cancer Biology 2023Quote: ... Equivalent cell numbers (∼3 × 106) across all samples were harvested in QIAzol lysis reagent (Qiagen). Illumina gene expression raw data were transformed using variance- stabilizing transformation (VST ...
-
bioRxiv - Neuroscience 2023Quote: ... the tissue was ground using a tissue lyser (Qiagen, 3 min at 300 s-1) in 200 µL 500 mM Tris pH 9 by adding a metal ball (diameter 5 mm) ...
-
bioRxiv - Genomics 2023Quote: ... 3) cleanup of final libraries with QIAgen MinElute PCR purification kit (QIAgen, Cat. No 28004), followed by a second cleanup with 2X SPRI ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were incubated for 3 minutes at 56°C in a deparaffinization solution (Qiagen, 19093). A step of digestion with Proteinase K was performed followed by a treatment with DNAses ...
-
bioRxiv - Molecular Biology 2023Quote: ... The supernatant was then incubated with 3 ml of Ni-NTA Superflow agarose beads (QIAGEN), which were preequilibrated with bacterial lysis buffer ...
-
bioRxiv - Immunology 2024Quote: ... Total RNA (3 independent samples per group) was isolated (QIAGEN miRNeasy mini extraction kit (QIAGEN) and cDNA was synthesized (High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems)) ...
-
bioRxiv - Immunology 2024Quote: ... Total RNA (3 independent samples per group) was isolated (QIAGEN miRNeasy mini extraction kit (QIAGEN) and cDNA was synthesized (High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems)) ...
-
bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was briefly incubated (approximately 5 min) with 3 ml Ni-NTA Agarose (QIAGEN) before flow-through was collected ...
-
bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified with Ni-NTA chromatography (Ni2+-nitrilotriacetate, Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: Initial screens were performed using Nucleix and Protein Complex suites (Qiagen) in a sitting-drop setup ...
-
bioRxiv - Microbiology 2021Quote: ... the proteins were purified on a Ni-NTA column (Qiagen Inc.), eluted with imidazole ...
-
bioRxiv - Biochemistry 2020Quote: ... E2 and polyPRM5 proteins were purified using Ni NTA-agarose (Qiagen) followed by cation exchange (Source15S ...
-
bioRxiv - Cancer Biology 2020Quote: ... The bacterial His-tagged proteins were purified under denaturing conditions (Qiagen) and refolded by dialysis as described previously (22) ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were immobilized on a glutathione agarose gravity flow column (Qiagen) washed 5 times with PBS solution and eluted in 50 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Plant Biology 2020Quote: GRF5 protein sequences were aligned using CLC DNA Workbench 8.0 (Qiagen) with the following settings ...
-
bioRxiv - Immunology 2021Quote: ... The proteins were captured using Ni-NTA (Qiagen, Germantown, MD, USA), washed in EQ buffer (25 mM Tris pH 8.0 ...
-
bioRxiv - Biophysics 2021Quote: ... Fusion proteins were purified from clarified lysate by Ni-NTA (Qiagen) affinity chromatography ...
-
bioRxiv - Biophysics 2020Quote: ... Proteins were purified using nickel-nitrilotriacetic acid (Ni-NTA) resin (Qiagen) and the histidine tag was subsequently removed by thrombin digestion (10 U/mg of protein) ...
-
bioRxiv - Microbiology 2022Quote: ... recombinant protein was initially purified by nickel affinity chromatography (Qiagen, Holland) and subsequent size exclusion using Superdex 200 increase (GE Healthcare ...
-
bioRxiv - Molecular Biology 2022Quote: ... proteins in the supernatants were purified using Ni-NTA beads (Qiagen) according to (62) ...
-
bioRxiv - Biochemistry 2021Quote: ... Protein was purified by FPLC using a Ni-NTA column (Qiagen), PBE-94 column (Sigma) ...
-
bioRxiv - Microbiology 2020Quote: ... The proteins were purified using Ni-nitrilotriacetic acid (Ni-NTA; Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... and RNA was extracted with DNA/RNA/Protein Mini Kit (Qiagen). The quality and quantity of RNA was assessed using the Agilent RNA 6000 Nano Kit Bioanalyzer (Agilent Technologies) ...
-
bioRxiv - Biophysics 2022Quote: ... The protein was purified from culture supernatant with Ni-NTA (Qiagen), biotinylated with in-house purified BirA enzyme ...
-
bioRxiv - Microbiology 2019Quote: ... Initial crystallization conditions were obtained with the Protein Complex Suite (Qiagen) screened by sitting drop-vapor diffusion at 18 °C using a Mosquito robot and were optimized in hanging drops by mixing 1 μL of the sample with 1 μL of the precipitant ...
-
bioRxiv - Cell Biology 2019Quote: ... The His-tagged protein was purified using Ni-NTA resin (Qiagen) following the manufacturer’s instructions and was used for rabbit immunization ...
-
bioRxiv - Genomics 2021Quote: ... The proteins were bound to a Ni-NTA sepharose beads (Qiagen), extensively washed with binding buffer and eluted in buffer containing 50 mM Tris-HCl pH 8 ...