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Citations for Qiagen :
601 - 650 of 1868 citations for Dengue Virus Serotype 3 Envelope Protein Mouse Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Protein was affinity-purified using Ni-NTA agarose (Qiagen #301210), and exchanged into severing buffer I (SBI ...
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bioRxiv - Biochemistry 2021Quote: ... Protein crystallization was screened for in commercially available screens (Qiagen), using a Crystal Gryphon robot (Art Robbins Instruments) ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified using Ni-NTA affinity columns (Qiagen) and subsequently purified by gel filtration using a HiLoad Superdex 75 pg column (GE) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then purified using Ni-NTA agarose (Qiagen 30210). The amount of purified protein was estimated both by conducting BCA assays (Thermo Fisher 23225 ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged proteins were purified with Ni2+-NTA beads (QIAGEN) and were eluted with 300 mM imidazole in PBS buffer ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... RNA was extracted using the Allprep RNA/Protein Kit (Qiagen). The quality and quantity of RNA were assessed using the Experion RNA Analysis Kit (BioRad ...
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bioRxiv - Bioengineering 2022Quote: ... Recombinant proteins were purified using Ni-NTA affinity resin (Qiagen) according to standard protocols (21) ...
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bioRxiv - Microbiology 2022Quote: ... His6-tagged protein was affinity purified using NiNTA resin (Qiagen), which was washed with binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... His6-tagged protein was affinity purified using NiNTA resin (Qiagen), which was washed with binding buffer ...
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bioRxiv - Immunology 2019Quote: ... Proteins were purified by affinity chromatography on NiNTA agarose (Qiagen), followed by FPLC on MonoQ (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was isolated by Ni-NTA affinity chromatography (Qiagen) and eluted with 50 mM Tris buffer pH 7.5 ...
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bioRxiv - Synthetic Biology 2020Quote: Protein purification was done using Ni-NTA Spin Columns (Qiagen) following the supplier recommendations ...
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bioRxiv - Cancer Biology 2020Quote: ... or the AllPrep DNA/RNA/Protein mini kit (Qiagen #80004) and quantified using 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were purified with Ni2+-NTA beads (Qiagen) and eluted with 300 mM imidazole in PBS buffer ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were purified using Ni-NTA agarose resin (Qiagen), washed with 20 mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: ... and His6-tagged protein was captured on NiNTA agarose (Qiagen). Following extensive washes with core buffer supplemented with 20 mM imidazole ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were affinity purified on Ni-NTA agarose beads (Qiagen). Proteins eluted from Ni-NTA beads by stepwise addition of increasing concentrations of 50-300 mM imidazole in a buffer containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2021Quote: ... and the resulting proteins were purified using Ni-resins (Qiagen) and size exclusion chromatography (HiLoad Superdex 75 ...
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bioRxiv - Immunology 2021Quote: ... and the resulting proteins were purified using Ni-resins (Qiagen). The vector encoding a hexa-His tag fused to a single chain homotrimeric mLIGHT extracellular domain (G73-V239 ...
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bioRxiv - Bioengineering 2022Quote: ... Pan-Fzd protein was purified using Ni-NTA Agarose (Qiagen), followed by biotinylation and size-exclusion chromatography with a Superdex S75 column (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: Protein phosphorylation assay was performed using PhosphoProtein Purification Kit (Qiagen), exactly as described by manufacture’s guideline ...
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bioRxiv - Cell Biology 2022Quote: ... KASH5 fusion proteins were purified through consecutive Ni-NTA (Qiagen), amylose (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged proteins were bound by Ni-NTA agarose (Qiagen) and eluted with 500 mM imidazole in HSB ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant proteins were then purified by Ni-NTA-agarose (Qiagen) affinity chromatography ...
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bioRxiv - Cancer Biology 2023Quote: ... for GST-tagged protein or Ni-NTA Agarose beads (QIAGEN) for His-tagged protein ...
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bioRxiv - Evolutionary Biology 2023Quote: ... for GST-tagged protein or on Nickel beads column (Qiagen) for 6XHIS-tagged protein ...
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bioRxiv - Biophysics 2023Quote: ... The recombinant proteins were purified using nickel affinity chromatography (Qiagen). As the final purification step ...
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bioRxiv - Plant Biology 2023Quote: ... Protein alignment was performed with CLC Genomics Workbench 22.0.1 (Qiagen) using MUSCLE v3.8.425 (Edgar ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were incubated with Ni-NTA Agarose (Qiagen, Cat#30230) resin for 30 min at 4 °C in a beaker with stirring ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified by Ni2+-affinity (Ni-NTA agarose, Qiagen) then passed over an anion-exchange column (Hitrap Q HP ...
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bioRxiv - Microbiology 2023Quote: Plasmids used for protein purification were based on pQE70 (Qiagen). For purification of EsaDEG ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... and extracted using AllPrep DNA/RNA/Protein Mini kit (Qiagen), as previously described102.
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bioRxiv - Immunology 2024Quote: ... The PELO protein was purified with Ni2+-NTA-agarose (Qiagen) and followed by further purification by Superdex 200 10/30 prepacked column (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... The protein was isolated using a Ni-NTA column (Qiagen). 25 μg of isolated protein was used to immunize mice by subcutaneous injection with complete Freund’s adjuvant and boosted three times with incomplete Freund’s adjuvant to generate PfATP4 antibody.
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bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with Ni-NTA Agarose Resin (Qiagen), followed by cation exchange chromatography using Source 15S Resin (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with Ni-NTA Agarose Resin (Qiagen), followed by cation exchange chromatography using Source 15S Resin (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with Ni-NTA Agarose Resin (Qiagen), followed by affinity-purification with amylose resin (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... His-tagged protein was bound to Ni-NTA beads (Qiagen). The beads were washed with 100 mL lysis buffer and protein was eluted with 40 mL elution buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... het and KO mice (3 each) by RNeasy Micro Kit with DNase I (Qiagen). Quality control was done by Qubit RNA assay ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were harvested after 3 or 7 days post-transfection (RNEasy mini kit, Qiagen), whereby cDNA was made and amplified via qPCR with primers for GAPDH ...
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bioRxiv - Immunology 2019Quote: ... Human RAC2 3’UTR was amplified with One Step Ahead RT-PCR kit (Qiagen) from human mRNA using the following primers RAC2 3’UTR+ ...
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bioRxiv - Systems Biology 2020Quote: ... 3 mL of culture were mixed with 6 mL of RNAprotect bacteria reagent (Qiagen) and processed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the media was collected and applied to 3 ml of Ni-NTA resin (Qiagen). The resin was collected and washed with PBS supplemented with 20 mM imidazole ...
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bioRxiv - Bioengineering 2020Quote: ... 0.001 M of HCl at pH 3 was added and then homogenized (TissueRuptor, Qiagen) on ice ...
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bioRxiv - Genetics 2021Quote: ... 3×105 U2OS cells were reverse transfected with 5 pmol of each siRNA (QIAGEN) using lipofectamine RNAiMAX (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: ... cells or tissues were lysed using a TissueLyser II (3 min, 30 Hz; Qiagen) in RIPA buffer (150 mM NaCl (Merck) ...
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bioRxiv - Plant Biology 2021Quote: Plant tissue was disrupted mechanically using 3 mm tungsten carbide beads (Qiagen, Hilden, Germany), and a TissueLyser II (Quiagen ...
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bioRxiv - Immunology 2019Quote: ... 3 times and then eluted with 350 uL of buffer RLT (Qiagen RNeasy kit). Input and immunoprecipitated RNA was purified on column (Qiagen RNeasy kit ...
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bioRxiv - Plant Biology 2022Quote: ... from Brachypodium Bd21-3 genomic DNA extracted using the DNeasy Plant Mini Kit (Qiagen) or specific plasmids using primers that contained Type IIS restriction sites and overhangs complementary to the respective entry module ...
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bioRxiv - Microbiology 2023Quote: ... contaminated DNA was digested by 3 times treatment with RNase-Free DNase Set (Qiagen), and the absence of DNA contamination in extracted RNA was confirmed by PCR.