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Citations for Qiagen :
601 - 650 of 10000+ citations for Rat Eukaryotic Translation Initiation Factor 4E Binding Protein 1 EIF4EBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Total RNAs were extracted from wild-type or DACR HAP-1 with the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA of TIE-1 was isolated using the DNeasy Blood and Tissue kit (Qiagen, Valencia, CA, USA) and used as a template for PCR reactions ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was prepared from 1 μg of total RNA using the QuantiTect RT Kit (Qiagen, Venlo, the Netherlands). Quantitative RT-PCR was performed using TB Green Premix ExTaq II (Takarabio ...
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bioRxiv - Genomics 2021Quote: ... The reaction was stopped by adding 1 μL 0.5 M EDTA (pH = 8.0) then cleaned up with MinElute PCR Purification Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and sgRNA-specific and universal primers (Table 1) and subsequently purified with the QIAquick PCR Purification Kit (Qiagen). sgRNAs mRNA was transcribed using AmpliScribe-T7 Flash Transcription kit (Epicentre) ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was isolated from 1 ml of cells from each culture using a miRNeasy kit (Qiagen, Germany) with some modifications ...
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bioRxiv - Microbiology 2022Quote: Total community DNA was extracted from 1 g of crushed rocks using DNeasy PowerSoil Pro Kit (Qiagen, German), quality checked by electrophoresis using a 1.5% agarose gel and Nanodrop spectrophotometer (Thermofisher ...
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bioRxiv - Molecular Biology 2022Quote: Whole RNA was extracted from over 1 × 10^5 cells using the QIAGEN RNeasy Micro kit (QIAGEN, 74004) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription reactions were performed with 1 μg of total RNA with the QuantiTect Reverse Transcription kit (Qiagen). Gene expression was measured using TaqMan™ master mix (Thermofisher ...
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bioRxiv - Genomics 2023Quote: ... Total RNA was isolated from 1 million cells per condition using the RNeasy Plus Mini Kit (Qiagen, 74104). mRNA was isolated using the Poly(A ...
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bioRxiv - Biochemistry 2023Quote: ... and grown in LB medium (ampicillin 50 μg mL−1) and plasmid purified (miniprep kit Qiagen, Hilden, Germany). Mutations were confirmed by DNA sequencing (Eurofins Genomics ...
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bioRxiv - Genomics 2023Quote: ... 1 μg total RNA was subjected to reverse transcription with a QuantiTect reverse transcription kit (Qiagen, Germantown, MD). The resulting cDNA and Fast SYBR green master mix (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... An amount of 1 µg of RNA was reverse transcribed using Omniscript Reverse Transcription (RT) Kit (Qiagen, #205113) together with random oligonucleotides (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell pellets were washed with 1× PBS and RNA were extracted using the RNeasy plus mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from 1 x 106 transduced HEK293T cells with the RNeasy Plus Mini Kit (Qiagen, #74134) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μL of bisulfite treated DNA was amplified for the regions of interest using PyroMark PCR kit (Qiagen) with final primer concentration of 0.4 μM ...
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bioRxiv - Immunology 2023Quote: ... Total DNA was obtained from the lysed inoculated THP-1 macrophages using DNeasy Blood & Tissue Kit (Qiagen, Germany). The amplification reaction was performed in a 20μl final volume containing 1μl of template DNA ...
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bioRxiv - Microbiology 2023Quote: ... 1 mL of culture was withdrawn and chromosomal DNA extracted using the Dneasy blood and tissue kit (Qiagen). The samples were normalized according to DNA concentrations and diluted 1:5 ...
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bioRxiv - Bioengineering 2023Quote: ... 1 μg of RNA per condition was used to retrotranscribe into cDNA QuantiTect® Reverse Transcription Kit (Qiagen). qPCR was performed in 386 wells plates in QuantStudio™ Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Biochemistry 2023Quote: ... Negatively supercoiled pHOT-1 DNA was purified from DH5α utilizing a Qiagen Plasmid Midi Kit (Qiagen, Hilden, Germany).
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bioRxiv - Systems Biology 2023Quote: ... Amplicons were gel-purifed from a 1 % Agarose gel using the QIAquick Gel Extraction Kit (QIAGEN, Düren, Germany). After determining the concentration with Qubit (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA extraction was carried out using one of the following methods: 1) QIAmp DNA Mini Kit (Qiagen, Germany); 2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was isolated from 1 mL of overnight culture of each genotype (DNeasy Blood & Tissue Kit; Qiagen). Paired-end ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from 1 x 108 trypanosome cells using the Qiagen RNeasy Mini Kit (Qiagen, Netherlands) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Up to 1 ug of RNA was converted into cDNA using either the Quantitect Reverse Transcription kit (QIAGEN) or the Superscript IV transcription kit (ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... V6.5 mESCs and K562 genomic DNA was purified from 1 million cells using the QIAamp micro kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from frozen Hepa 1-6 cell pellets using the RNeasy Mini kit (74106, QIAGEN) following the manufacturer’s instructions with an on-column DNase digestion step (79254 ...
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bioRxiv - Immunology 2024Quote: ... Reverse transcription was performed with 1 µg of template RNA using the Qiagen QuantiTect reverse transcription kit (Qiagen) in a Bio-Rad T100TM Thermal Cycler (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was then purified from 500,000 to 1 million cells using the miRNeasy Micro kit (Qiagen GmbH) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... a minimum of 1 x106 cells per experimental group were processed using the RNeasy Mini kit (#74104; QIAGEN). The mRNA levels were measured with the NanoDrop (DS-11 ...
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bioRxiv - Physiology 2024Quote: ... using 350 microliter RLT buffer with 1% b-mercaptoethanol as a collection/lysis buffer (RNeasy micro kit, Qiagen). RNA was extracted following manufacturer’s instructions (RNeasy micro kit ...
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bioRxiv - Genomics 2019Quote: The RNA extraction kit (RNeasy Mini Kit, Qiagen) was used for the total RNA isolation ...
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bioRxiv - Bioengineering 2020Quote: ... An RNA extraction kit (RNeasy extraction Kit, Qiagen) was used to purify RNA ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription kits (RT2 First Strand Kit; Qiagen) were used for cDNA synthesis ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was purified using Ni-NTA Fast Start (Qiagen, #30600) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2021Quote: ... refolded protein mixture was loaded on Ni-NTA beads (Qiagen) as 1ml per 2 liter culture ...
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bioRxiv - Developmental Biology 2020Quote: ... recombinant proteins were purified using Ni-NTA Agarose (Qiagen 30210). Buffer was exchanged by dialysis to PBS ...
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bioRxiv - Microbiology 2019Quote: ... His-tagged proteins were purified over Ni-NTA-agarose (Qiagen) with 3 wash steps at 30 mM imidazole and elution with 250 mM imidazole in denaturing buffer ...
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bioRxiv - Neuroscience 2019Quote: ... for protein extraction or 200 µL of RLT buffer (Qiagen) for RNA extraction and stored at −80°C until further sample processing.
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bioRxiv - Biochemistry 2019Quote: ... Proteins were purified by Ni2+-affinity (Ni-NTA agarose, Qiagen) then passed over an anion-exchange column (Hitrap Q HP ...
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bioRxiv - Cell Biology 2019Quote: ... Protein was affinity-purified using Ni-NTA agarose (Qiagen #301210), and exchanged into severing buffer I (SBI ...
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bioRxiv - Biochemistry 2021Quote: ... Protein crystallization was screened for in commercially available screens (Qiagen), using a Crystal Gryphon robot (Art Robbins Instruments) ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified using Ni-NTA affinity columns (Qiagen) and subsequently purified by gel filtration using a HiLoad Superdex 75 pg column (GE) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then purified using Ni-NTA agarose (Qiagen 30210). The amount of purified protein was estimated both by conducting BCA assays (Thermo Fisher 23225 ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged proteins were purified with Ni2+-NTA beads (QIAGEN) and were eluted with 300 mM imidazole in PBS buffer ...
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bioRxiv - Bioengineering 2022Quote: ... Recombinant proteins were purified using Ni-NTA affinity resin (Qiagen) according to standard protocols (21) ...
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bioRxiv - Microbiology 2022Quote: ... His6-tagged protein was affinity purified using NiNTA resin (Qiagen), which was washed with binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... His6-tagged protein was affinity purified using NiNTA resin (Qiagen), which was washed with binding buffer ...
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bioRxiv - Immunology 2019Quote: ... Proteins were purified by affinity chromatography on NiNTA agarose (Qiagen), followed by FPLC on MonoQ (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was isolated by Ni-NTA affinity chromatography (Qiagen) and eluted with 50 mM Tris buffer pH 7.5 ...