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Citations for Qiagen :
501 - 550 of 10000+ citations for Rat Eukaryotic Translation Initiation Factor 4E Binding Protein 1 EIF4EBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Primary mouse CD4+ T cells were purified from lymph nodes and spleens by negative selection using anti-MHCII and anti-CD8 hybridoma supernatants (M5/114 and 2.43, respectively) and anti-rat Ig magnetic beads (Qiagen BioMag). The resulting CD4+ T cells were then immediately activated on 24-well plates coated with anti-CD3 and anti-CD28 (1 ug/ml each ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were purified using Ni-NTA agarose (Qiagen) and eluted using 300 mM imidazole in purification buffer ...
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bioRxiv - Genetics 2020Quote: ... Proteins were purified with Ni-NTA beads (Qiagen). Proteins and beads were washed 3 times with protein purification lysis buffer before incubating the beads with elution buffer (400 mM imidazole in protein purification lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was purified using Ni-NTA agarose (Qiagen) eluting with 300 mM imidazole ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were purified with Ni-NTA Resin (Qiagen) using standard protocols ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were purified using Ni-NTA agarose (Qiagen) resin first ...
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bioRxiv - Biochemistry 2022Quote: ... Protein Center (Thermo) and Ingenuity Pathways Analysis (Qiagen). For protein center analysis ...
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bioRxiv - Synthetic Biology 2022Quote: ... Proteins were purified on Ni-NTA resin (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with NiNTA agarose (Qiagen). The eluate was further purified over a Source 15 Q column (Cytiva) ...
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bioRxiv - Immunology 2023Quote: ... Proteins were purified using Ni-NTA agarose (QIAGEN), followed by size-exclusion chromatography using HiLoad 16/600 Superdex200 columns (GE Healthcare Life Sciences) ...
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bioRxiv - Plant Biology 2023Quote: ... The proteins were purified using Ni-NTA (Qiagen), and the His tag was cleaved by the Tev protease ...
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bioRxiv - Microbiology 2024Quote: ... SrtC2 protein was purified using Ni-NTA (Qiagen) affinity chromatography with the addition of 5 mM β-mercaptoethanol in all buffers ...
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bioRxiv - Neuroscience 2024Quote: ... to obtain protein lysates or RLT buffer (Qiagen) for RNA isolation.
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bioRxiv - Neuroscience 2022Quote: ... dsDNA binding dye (SYBR® green) chemistry-based qPCRs were performed on purified RNA samples using Rat GABA & Glutamate RT2 Profiler PCR arrays (Qiagen, Inc. ...
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bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA (gDNA) was extracted from 1-5×106 cells using DNeasy Blood and Tissue Kit (Qiagen) and 250 ng gDNA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted from 1 ml of overnight culture using a QIAamp DNA Mini Kit (Qiagen). Illumina MiSeq sequencing operated by the Functional Genomics Centre Zurich and Novogene (Cambridge ...
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bioRxiv - Immunology 2021Quote: DNA was isolated from 1 × 106 PBMC using the DNeasy Blood & Tissue Kit (Qiagen, Cat No 69506), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from CTi400 and N/TERT-1 cells using RNeasy Plus mini kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Total RNA from PGC fibroblast and DF-1 cells were extracted using an RNeasy mini kit (Qiagen), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×105 cells were harvested and total RNA was extracted using the RNeasy Plus Mini kit (Qiagen). cDNA was synthesized from 0.1 μg total RNA using Superscript IV reverse transcriptase (Invitrogen ...
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bioRxiv - Genetics 2020Quote: ... cDNA synthesis was performed with 1 mg of total RNA using miScript II Reverse Transcription Kit (QIAGEN). See Supplemental Information for primers used in these experiments ...
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bioRxiv - Synthetic Biology 2021Quote: mESC gDNA was purified from 1-5 million cells using the QIAamp DNA mini kit (Qiagen 51306) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from 1 × 108 trypanosome cells using the Qiagen RNeasy Mini Kit (Qiagen, Netherlands) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Whole RNA (1-3 μg total per 10 μL volume) was isolated using RNAeasy mini kit (QIAGEN) or TRIzol (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... amplicons were purified from excised gel slices (1% agarose) using the Qiagen MinElute Gel Extraction kit (Qiagen). Each amplicon was quantified using Quant-IT HS reagents (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: The obtained ~2Kb amplicons were gel purified from 1% agarose gel using QIAquick Gel Extraction Kit (Qiagen) and cloned in pBluescript SK (+ ...
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bioRxiv - Neuroscience 2020Quote: The obtained ~2Kb amplicons were gel purified from 1% agarose gel using QIAquick Gel Extraction Kit (Qiagen) followed by clean-up with QIAquick PCR purification kit (Qiagen) ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots of 1 μg of RNA were reverse transcribed using a QuantiTect Reverse Transcription kit (Qiagen, 205314), and qPCR was performed with iTaq SYBR Green (Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1-2 µg of RNA was used for the cDNA synthesis (Omniscript RT Kit (Qiagen, 205111)) ...
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bioRxiv - Molecular Biology 2022Quote: ... including of multiple steps showed below: 1) Total DNA was extracted using QIAamp DNA Mini Kit (Qiagen); 2 ...
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bioRxiv - Developmental Biology 2022Quote: ... 1000:1 v/v) and DNA from individual colonies were isolated using QIAprep Spin Miniprep Kit (Qiagen).
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bioRxiv - Genetics 2022Quote: Total RNA was extracted from stably transduced THP-1 cultures using the RNeasy Plus Mini kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and a Cyprus wheatear (O. cypriaca) (Table 1) using the MagAttract HMW DNA kit (Qiagen, Hilden, Germany). Digestion was performed in 360 ul of buffer ATL ...
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bioRxiv - Systems Biology 2019Quote: ... RNA was extracted from more than 1 × 107 cells using RNeasy Plus Mini Kit (Qiagen, Valencia, CA). Three biological replicates were prepared for each condition ...
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bioRxiv - Neuroscience 2020Quote: ... DNA was isolated from 1-2 mm tail tissue using the DNeasy Blood and Tissue Kit (Qiagen) following the protocol for isolation of DNA from animal tissues and was eluted in 100 µl H2O ...
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bioRxiv - Immunology 2020Quote: ... Isolation of RNA from THP-1 cells was carried out with a RNeasy Plus Mini kit (Qiagen). The High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Genomics 2021Quote: ... In brief we perform bisulfite conversion on 1 µg of genomic DNA using EpiTech kit from Qiagen according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 2.5ug of the plasmid of choice was transfected into HEK293T cells together with 1 ug pVSV-G and 1.5 ug pCMVΔR8.2 using an Effectene transfection kit (Qiagen). Twenty-four hours after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting products were run on 1 % agarose gel then extracted using QIAquick Gel Extraction Kit (Qiagen). Restriction enzymes and Phusion DNA polymerase were purchased from New England BioLabs Inc and used according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... cDNA was prepared from 0.5-1 μg total RNA with the QuantiTect reverse transcription kit (205311; Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: The small RNAs from the yeast cells (1 x 107) were isolated with the miRNeasy kit (Qiagen) with modifications ...
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bioRxiv - Immunology 2021Quote: ... Cells were sorted in RTL Lysis Buffer plus 1% β-mercaptoethanol (74134, RNeasy Plus Mini Kit; QIAGEN), or in sterile Sorting Medium [RPMI 1640 supplemented with 10% (v/v ...
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bioRxiv - Genetics 2019Quote: ... Genomic DNA from 10 Ag(PMB)1 individuals was extracted using the Blood and Tissue Kit (Qiagen). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... RNA extraction was performed from 1 mL of the bacterial solution using an RNeasy Mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of gDNA was extracted from WILD-seq transduced cells (Blood&Cell Culture DNA Kit, Qiagen) and the barcode amplified by PCR using primers containing Illumina-compatible adapters (gDNAWhitelist_Fwd/Rev) ...
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bioRxiv - Cell Biology 2022Quote: ... First strand cDNA was synthesized from 1 μg total RNA using the QuantiTect Reverse Transcription Kit (Qiagen). RT-qPCR was performed in a LightCycler480 (Roche ...
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bioRxiv - Genomics 2022Quote: ... DNA from I-73-1 and D308 was extracted using a ‘Genomic-tip 100/G’ kit (Qiagen) and long-reads sequenced with PacBio RS II at 100x coverage ...
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bioRxiv - Microbiology 2022Quote: ... Ligation of 1 µl 5′RACE PCR products were performed with the Qiagen PCR cloning kit (Qiagen) according to the manufacters protocol ...
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bioRxiv - Immunology 2022Quote: cDNA was generated from 1 µg RNA with the Omniscript® Reverse Transcription Kit (Qiagen, Hilde, Germany) using oligoDT12-18 primer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2024Quote: ... total DNA from 1×106 cells was extracted using the Blood & Cell Culture DNA Mini Kit (Qiagen). Extracted DNA was diluted to 10 ng/μl and subjected to qPCR analysis ...