Labshake search
Citations for Qiagen :
601 - 650 of 1363 citations for Mouse Anti Hepatitis C Virus NS5a Antibody 1827 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: RNA from whole mouse OE samples was extracted using RNeasy kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: Genomic DNA from mouse blood was extracted using a DNeasy Blood & Tissue Kit (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: RNA was extracted from cells and mouse tumors using the miRNeasy kit (Qiagen, #217004) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... L1-L5 mouse DRG was homogenized with an RNeasy Mini Kit (Qiagen, Valencia, CA) using on-column DNase-I digestion according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... spongiae DNA was extracted from mouse tissues using DNAeasy Blood and Tissue kit (Qiagen). The numbers of mice used in each individual experiment were calculated to permit detection of at least a two- to four-fold difference in bacterial loads between groups with 95% (two-sided ...
-
bioRxiv - Microbiology 2024Quote: Mouse cDNA was amplified with gene-specific primers and HotStarTaq Master Mix Kit (Qiagen) (Table 1) ...
-
bioRxiv - Molecular Biology 2024Quote: RNA was extracted from mouse islets using the RNAeasy Micro Kit (QIAGEN, Cat # 74004); RNA was extracted from flash-frozen liver and peripancreatic fat tissues using TRIzolTM (Invitrogen ...
-
bioRxiv - Cell Biology 2024Quote: Total RNA was purified from mouse tissues using the RNeasy Plus Mini Kit (Qiagen) and then transcribed into cDNA using the iScript™ cDNA Synthesis Kit (Bio-Rad) ...
-
bioRxiv - Pathology 2024Quote: Mouse kidney outer cortex tissues were dissected and homogenized in QIAzol (QIAGEN, Germantown, MD). Total RNA samples were extracted using RNeasy Plus Universal Kit (QIAGEN ...
-
bioRxiv - Biophysics 2019Quote: ... Lysates were spun for a further 20 min at 40,000 g at 4 °C and resulting supernatants were incubated 1 hour with Ni2+-nitrilotriacetate (NTA) agarose beads (QIAGEN) at 4 °C on rotating wheel ...
-
bioRxiv - Microbiology 2020Quote: ... Recombinant both N-and C-terminally His-tagged proteins were purified using a Ni-NTA Superflow cartridge (1ml, Qiagen) and the Fast Protein Liquid Chromatography (FPLC ...
-
bioRxiv - Microbiology 2019Quote: ... Cells were washed with PBS and incubated at 37°C with a 1:100 dilution of RNase A (QIAGEN) in PBS for 3 hours ...
-
bioRxiv - Immunology 2021Quote: The indicated cell types were harvested and stored at −80°C until RNA was isolated using RNeasy kit (Qiagen) following manufacturer’s instructions ...
-
bioRxiv - Genomics 2022Quote: ... supplemented with 100 μg/mL of carbenicillin for 16 hr at 30°C followed by plasmid purification (Qiagen, 12991).
-
bioRxiv - Biochemistry 2020Quote: ... Lysates were centrifuged at 30,597xg and 4°C for 20min and the supernatant was bound to Ni-NTA Agarose resin (Qiagen), washed with 10 CV of wash buffer (50 mM HEPES-KOH pH=7.5 ...
-
bioRxiv - Cancer Biology 2020Quote: DNA isolation was performed by adding proteinase K and incubation at 56°C using QIAamp DNA Mini Kit (Qiagen). DNA was quantified using a Qubit Fluorometer (ThermoFisher) ...
-
bioRxiv - Genetics 2019Quote: Single cells meant for RNA processing were sorted into 96-well full-skirted Eppendorf plates that were pre-chilled at 4°C and were prefilled with 10μL of lysis buffer consisting of TCL buffer (Qiagen) supplemented with 1% beta-mercaptoethanol ...
-
bioRxiv - Microbiology 2019Quote: ... Cell lysates were then incubated at 4 °C for 1-1.5 hours on one ml of Ni-NTA resin (Qiagen). The resin was then loaded into columns (Biorad ...
-
bioRxiv - Genetics 2020Quote: ... stored at −80°C) and PBMCs were extracted using QIAGEN DNeasy Blood and Tissue kit (Qiagen Sciences, Maryland, USA), following manufacturer’s guidelines ...
-
bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared using a JA-20 rotor (18,000 rpm for 30 min at 4°C) and incubated with Ni-NTA superflow resin (Qiagen) for 30 min ...
-
bioRxiv - Cell Biology 2021Quote: Tissue lysates were further homogenized and centrifuged at 10,000 g for 10 min at 4°C in microcentrifuge spin columns (QIAshredder, Qiagen) to obtain clear protein lysate ...
-
bioRxiv - Neuroscience 2020Quote: ... Total RNA was isolated from a bank of frozen brains kept at −80 °C using a RNA isolation kit according to the manufacturer’s instructions (Qiagen). Frozen brain samples were thawed and dissected from 5 different regions ...
-
bioRxiv - Plant Biology 2021Quote: ... the protein was removed by 1.1 μL of proteinase K (20mg/ml) at 45° C for 1h and RNA by 2μL of RNaseA (1mg/ml) (Qiagen) digestion at 37°C for 1h ...
-
bioRxiv - Microbiology 2021Quote: ... Membrane filters were stored at −80°C until DNA could be extracted using a DNeasy Power Water kit (Qiagen) and bacteria were quantified using qPCR of the vvhA gene.
-
bioRxiv - Plant Biology 2020Quote: ... 10mM imidazole was added and incubated on rotator for overnight at 4°C in the presence of 2.5ml of Ni-NTA beads (Qiagen). The beads were washed with EB containing 6M guanidine-HCl and 0.25% Triton X-100 ...
-
bioRxiv - Microbiology 2020Quote: ... The supernatant obtained by 5 minutes of centrifugation at 16.000 g of bacterial lysate was stored at −80 °C or immediately purified with the miRNeasy mini kit (Qiagen). RNA quantity was determined using the Qubit 4 Fluorometer and Nanodrop spectrophotometer (Thermofisher) ...
-
bioRxiv - Microbiology 2020Quote: ... Crystals of Brd4(BD1) with YFV-C (1-10) K4ac peptide were grown from a solution containing 0.2 M sodium chloride (QIAGEN), 0.1 M Tris (pH 7.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer A supplemented with 200 mM imidazole in gravity flow ...
-
bioRxiv - Molecular Biology 2022Quote: ... counted and PBS washed pellets were stored at -80 °C for subsequent gDNA extraction (QIAamp DNA Mini, QIAGEN 51304). DNA content was determined with Qubit dsDNA HS Kit (Thermo Fisher Q32854 ...
-
bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting with buffer A supplemented with 250 mM imidazole in gravity flow ...
-
bioRxiv - Biochemistry 2022Quote: ... 0.5 mM GDPNP-Mg2+) were obtained at 4°C using 0.2 M Lithium nitrate and 20% PEG3350 (PEG Suite I, Qiagen) as precipitating agent ...
-
bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B supplemented with 250 mM imidazole ...
-
bioRxiv - Biochemistry 2022Quote: ... cleared by centrifugation (20 min, 20,000 × g, 4°C) and the clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer C supplemented with 200 mM imidazole ...
-
bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA agarose resin (QIAGEN) eluted with buffer A supplemented with 0.5 mM TCEP and 200 mM imidazole ...
-
bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and cleared lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B (20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Systems Biology 2022Quote: ... Ten millilitres of culture were pelleted by immediate centrifugation (5,000 × g for 3 min at 4°C) and resuspended in 5 mL of RNAlater (76106; Qiagen). Samples were stored at 4°C overnight ...
-
bioRxiv - Molecular Biology 2022Quote: ... The lysates were then incubated at 95°C for 5 min and filtered using a QIAshredder homogenizer (Qiagen, 79656). The extracted proteins were analyzed using 5–20% gradient sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE ...
-
bioRxiv - Microbiology 2022Quote: ... and stored pellets at −80 °C for <1 week before RNA extraction using RNeasy Protect Bacteria Mini Kit (Qiagen) according to the manufacturer’s instructions (protocol #5) ...
-
bioRxiv - Immunology 2020Quote: ... ConM-SOSIP.v7 carrying a C-terminal His-tag (diluted to 6.5nM in TBS) was immobilized onto 96-well Ni-NTA ELISA plates (Qiagen) by incubation for 2 hours at room temperature after which the plates were washed 3 times with TBS ...
-
bioRxiv - Biophysics 2020Quote: ... Debris was removed by centrifugation (20,000×g, 30 min, 4°C) and the supernatant was incubated with Ni-NTA resin (Qiagen) for 30 min at 4°C ...
-
bioRxiv - Microbiology 2019Quote: DNA was extracted from subsamples frozen at -20°C using the DNeasy PowerLyzer PowerSoil kit (Qiagen, Germantown, MD, USA), according to the manufacturer’s instructions with minor modifications to increase yield and final DNA concentration based on the assumption that some sites and depths would have a relatively low microbial biomass ...
-
bioRxiv - Molecular Biology 2020Quote: ... was tagmented with Tn5 transposase (25 μM) for 30 min at 55°C and purified using MinElute columns (QIAGEN).
-
bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 30 min at 45,000 × g at 4°C and incubated with 1 ml of Ni-NTA agarose beads (Qiagen) per 1 l of expression culture for 2 h rotating at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... with 0.5% NaCl at 30 °C for overnight and DNA extraction was done by DNeasy Blood and Tissue Kit (QIAGEN). Specific forward and reverse primers targeting each gene of interest were used for PCR amplification (TABLE 2) ...
-
bioRxiv - Immunology 2019Quote: ... and transferred to −80°C storage until RNA purification with the Qiagen AllPrep RNA/DNA kit (Qiagen, Hilden, Germany). For generating stimulated PBMC samples ...
-
bioRxiv - Plant Biology 2019Quote: Genomic DNA was extracted immediately upon UV-C exposure (time point 0) using the Plant DNA Extraction kit (Qiagen). Five µg of genomic DNA were sonicated (Diagenode Bioruptor ...
-
bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation for 1 h at 4°C at 10,000 rcf before being subjected to Ni-NTA (Qiagen) affinity purification following the manufacturer’s protocol.
-
bioRxiv - Molecular Biology 2021Quote: ... The solution was further cleared at 20,000 × g at 4 °C and the supernatant was incubated with Ni-NTA resin (Qiagen) for 2 h at 4 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were expressed in BL21de3 E.coli by auto induction in LB medium at 18°C and purified using Ni-NTA beads (Qiagen). When present ...
-
bioRxiv - Microbiology 2020Quote: ... at 37°C and genomic DNA was extracted using the Qiagen DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA). The concentration of resultant DNA was determined using a Qubit double-stranded DNA BR assay kit and a Qubit fluorometer (ThermoFisher Scientific ...