Labshake search
Citations for Qiagen :
551 - 600 of 1675 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: ... purified using a Plasmid Maxi Kit (Qiagen 12163) and verified by Sanger sequencing prior to egg injection ...
-
bioRxiv - Molecular Biology 2020Quote: ... DNA was isolated from rat blood cells using QIAmp DNA Mini Kit (Qiagen, City, state) following manufacturer’s instructions using a QIAcube automated device ...
-
bioRxiv - Microbiology 2021Quote: ... Parasite cultures were synchronized by repeated sorbitol treatment and 100 μg of purified plasmid DNA (Plasmid Midi Kit, Qiagen, Valencia, CA) was transfected in P ...
-
bioRxiv - Cancer Biology 2019Quote: ... Bacterial cells were pelleted by centrifugation at 6,000 RCF at 4°C for 15 minutes and plasmid DNA was extracted using a Qiagen Plasmid Plus Midi Kit (Qiagen #12943). To estimate the total number of unique barcodes present in the plasmid pool ...
-
bioRxiv - Plant Biology 2019Quote: ... 50,000 protoplasts in a volume of 100 μL were transformed with 20 μg of each plasmid (2 μg/μL) purified using the Plasmid Maxi kit (Qiagen, Germany). One batch of protoplasts was treated with an equivalent amount of water and used as the negative (untransformed ...
-
bioRxiv - Physiology 2021Quote: ... Crtc2 and GFP expression plasmids were purified from DH5a Escherichia coli cultures using an EndoFree plasmid kit (Qiagen, Valencia, CA, USA), and resuspended in 71 mM sterile PBS ...
-
bioRxiv - Microbiology 2021Quote: Plasmid DNA isolation was performed on individual sewage pellets (420 mg) using Plasmid Purification Mini Kit (Qiagen, Cat No./ID: 12123) with QIAGEN-tip 100 (Qiagen ...
-
bioRxiv - Neuroscience 2021Quote: ... The bacterial colonies were cultured with Zeocin selection at large scale and maxi-plasmid preparations were carried out using HiSpeed Plasmid Maxi Kit (Qiagen, 12662). CpG Methyltransferase ...
-
bioRxiv - Cancer Biology 2022Quote: ... we cultured 250 ml Escherichia coli bacteria bearing transformed plasmids and isolated the plasmids using Qiagen Maxi-Prep Endotoxin-free kit (Qiagen; 12362) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Positive plasmids were extracted from overnight liquid cultures obtained from replica colonies by using the Plasmid Mini kit (Qiagen, Germantown, MD), and two to five clones for each strain were sequenced to ensure sequence fidelity to the previously cloned templates (35) ...
-
bioRxiv - Molecular Biology 2019Quote: ... The cloned plasmid was amplified in DH5-Alpha cells and purified with QIAfilter™ Plasmid Maxi Kit (Qiagen, catalogue number 12243). Plasmid concentration was measured using Qubit (Thermofisher ...
-
bioRxiv - Synthetic Biology 2022Quote: ... After overnight growth colonies were scraped into a collection bottle and plasmid pools were extracted using a plasmid maxiprep kit (Qiagen #12662). 2 smaller plates of LB with carbenicillin were also prepared with 1:20 diluted cells to count colonies and confirm transformation efficiency ...
-
bioRxiv - Biochemistry 2022Quote: ... pTripZ-M1 plasmids containing PRC1 subunit fusion gene and the puromycin selective marker as well as the pTripZ-G2-YFP-CBX2(ΔTRE) plasmid harboring a geneticin selective marker were purified according to the protocol listed in EndoFree Max Plasmid Kit (QIAGEN; 12362). 24-hours before transfection ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The recombinant plasmid was then purified at a high concentration (200 μg/mL) using the plasmid preparation kit (Qiagen, Germantown, MD).
-
bioRxiv - Developmental Biology 2023Quote: ... The cells containing the desired plasmids were amplified by broth culture and the plasmids were prepared by maxiprep kit (Qiagen, 12663). The plasmids were stored at −20°C until use.
-
bioRxiv - Immunology 2023Quote: ... A 1.5ml aliquot of Stbl2 cells from overnight culture was used for miniprep plasmid extraction (QIAGEN Plasmid mini kit, Cat. 12123), to check the EEV sequence ...
-
bioRxiv - Synthetic Biology 2023Quote: The ASKA-clone encoding tyrS was isolated from AG1 carrying this plasmid using the Qiagen Plasmid Midi (Qiagen Inc., CA, USA) and transformed into BL21-AI ...
-
bioRxiv - Cancer Biology 2023Quote: ... The DDR-CRISPR library plasmid DNA was purified from transformed bacterial culture using a Qiagen EndoFree plasmid DNA Maxi kit (Qiagen, #12362).
-
bioRxiv - Microbiology 2024Quote: ... Plasmid DNA was extracted using either a QIAGEN HiSpeed® Plasmid Midi Kit or Maxi Kit (QIAGEN Sciences, Germantown, MD, USA) as per the manufacturer’s instructions (HiSpeed® Plasmid Purification Handbook) ...
-
bioRxiv - Bioengineering 2024Quote: Plasmid DNA for pT2KXIGΔin (Ef1α:GFP expression vector) and pCS-ZT2TP (Tol2 plasmid) was prepared using the Endo-Free Plasmid Maxi kit (Qiagen, Germantown, MD) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... 4×106 HEK293T cells or 2×106 HeLa cells seeded in a 100 mm dish were transfected with 10μg of each plasmid purified using the endotoxin-free plasmid isolation kit (Qiagen, Germantown, MD, USA) with TransIT-293 (Mirus ...
-
bioRxiv - Microbiology 2022Quote: ... A wild-type virus stock NL4-3 was prepared by transfection of the pNL4-3 plasmid (purified using the Qiagen Plasmid Maxikit) into HeLa cells ...
-
bioRxiv - Microbiology 2021Quote: ... Isolates were cultured overnight on sheep blood agar and plasmid DNA was extracted using Qiagen Plasmid Midi kit (Qiagen Inc, Germantown, MD) as per manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... coli cells were cultured at 37°C and plasmid isolation was carried out using a Qiagen Plasmid Mini Kit (Qiagen, Germantown, MD) according to manufacturer instructions ...
-
bioRxiv - Microbiology 2023Quote: ... 293A cells were transfected with the plasmid of pCDNA3.1-ACE2 (human)-3×FLAG (MiaoLing Plasmid Platform, China) using polyfect (QIAGEN, West Sussex, UK) according to the manufacturer’s protocol ...
-
bioRxiv - Genetics 2024Quote: ... up to 750 ng of total DNA (containing the mutagenic enzyme and/or gRNA plasmid and the MTX resistance plasmid) was transfected in 0.75 million cells using the Effectene Transfection Reagent from Qiagen (cat. No 301425) in 24-well plates ...
-
bioRxiv - Molecular Biology 2020Quote: ... Plasmid transfections were achieved using Effectene transfection reagent (Qiagen) according to the manufacture’s protocol.
-
bioRxiv - Cell Biology 2020Quote: ... followed by plasmid miniprep using QIAprep Spin Miniprep (Qiagen) according to manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: ... aliquots were pooled prior to plasmid purification (Qiagen, 12963). For each of the aliquots ...
-
bioRxiv - Microbiology 2021Quote: ... using QIAGEN Plasmid Mini and Midi Kit (Qiagen, Germany) and subjected for Sanger sequencing (Eurofins ...
-
bioRxiv - Microbiology 2022Quote: ... Plasmid preparations (Qiagen mini prep kit Cat no.27106) were sequenced using plasmid-specific primers (Table S5) ...
-
bioRxiv - Cell Biology 2022Quote: Plasmid transfection was implemented using the Effectene reagent (Qiagen) according to the manufactures’ protocols ...
-
bioRxiv - Molecular Biology 2019Quote: ... Plasmids were prepared using QIAprep Miniprep Kit (Qiagen 27106) and all sequencing was performed using the Sanger method.
-
bioRxiv - Microbiology 2019Quote: ... Plasmids for transfection were prepared by Midi-preps (QIAGEN) and ethanol precipitated ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... by injecting the pUAST.attB-SlitOR5 plasmid (Endofree prepared, Qiagen) into fly embryos with the genotype y1 M{vas-int.Dm}ZH-2A w* ...
-
bioRxiv - Microbiology 2019Quote: ... and plasmids were isolated using QIAprep Miniprep kit (Qiagen).
-
bioRxiv - Neuroscience 2020Quote: ... Positive colonies were purified (QIAGEN, Plasmid Mini Prep Kit), confirmed by Sanger sequencing and prepared for injection (QIAGEN ...
-
bioRxiv - Cell Biology 2021Quote: ... Plasmid was isolated using a QIAprep Miniprep kit (Qiagen) and sequenced using M13 sequencing primers (forward primer ...
-
bioRxiv - Microbiology 2020Quote: ... Plasmids from single colonies were isolated (Qiagen Miniprep Kit) and screened for the algT promoter insertion using oAC32/oAC33 ...
-
bioRxiv - Microbiology 2021Quote: ... and plasmids were extracted by Mini prep (Qiagen # 27106) according to the manufacturer’s protocol.
-
bioRxiv - Molecular Biology 2020Quote: ... Plasmids were isolated using commercially available miniprep columns (Qiagen). Presence of the Cys mutation at selected positions in env was confirmed by Sanger sequencing (at Macrogen ...
-
bioRxiv - Microbiology 2022Quote: ... Plasmids were isolated from each sample by miniprep (Qiagen). The selection endpoint samples and the plasmid library input were prepared for sequencing as follows.
-
bioRxiv - Molecular Biology 2020Quote: ... Plasmids were extracted using QIAgene Miniprep kit (QIAGEN #27104), following the manufacturer’s protocol.
-
bioRxiv - Microbiology 2021Quote: ... coli with the Qiagen mini plasmid preparation kit (Qiagen). Pneumococcal DNA was isolated by phenol/chloroform extraction and ethanol precipitation from an exponentially growing culture in ThyB media (30 mg/ml Todd-Hewitt Broth powder and 0.2 mg/ml yeast) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Plasmids were prepared using QIAprep Miniprep Kit (Qiagen 27106). Constructs were confirmed by restriction digest and Sanger sequencing ...
-
bioRxiv - Molecular Biology 2022Quote: ... Plasmid DNA was recovered using a miniprep kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2020Quote: ... Plasmids were extracted using QIAprep Spin Miniprep Kit (Qiagen). Concentration was measured on a NanoDrop 2000 Spectrophotometer (Thermo) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The plasmids were prepared using the Maxiprep kit (Qiagen) and digested with XbaI overnight ...
-
bioRxiv - Bioengineering 2021Quote: ... and extracted by the Plasmid Plus Midi Kit (Qiagen) following the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Plasmid DNA for each parent was mini-prepped (Qiagen) and all parent plasmid DNA was subsequently pooled ...