Labshake search
Citations for Qiagen :
551 - 580 of 580 citations for Polyoxymethylene Homopolymer 20% PTFE Fiber Filled Granule since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Total RNA extraction from ∼20 mg of tissue was performed using Trizol reagent followed the RNeasy Plus Mini kit (Qiagen cat # 74134) according to the manufacturer instructions ...
-
bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from either 2D or matrigel-on-top (MoT) 3D (protocol for MoT 3D culture is detailed in (20)) cultures using RNeasy mini kit (Qiagen, catalog #74104) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... after which each lysate is transferred into an eppendorf tube containing 40 μL of 20 mg/mL RNAse A prepared from 100 mg/mL RNAse A (Qiagen, Cat# 19101). Using separate scalpel blades (Integra Miltex Sterile Safety Scalpels ...
-
bioRxiv - Genomics 2024Quote: ... five NHGRI-1 zebrafish pairs were crossed for each timepoint and three pools of embryos (20 embryos each) collected for whole RNA extraction using the RNeasy kit (Qiagen, Hilden, Germany) with gDNA eliminator columns for DNA removal ...
-
bioRxiv - Biochemistry 2024Quote: Total RNAs were extracted from control and lithium acetoacetate-treated (20 mM for 24 h) HEK293T cells using the RNeasy Plus Mini Kit (Qiagen, Cat# 74134). Three biological replicates were performed for each condition ...
-
bioRxiv - Molecular Biology 2020Quote: ... was digested overnight at 56°C in 20 ul proteinase K and 180 ul Buffer ATL from the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Valencia, California, USA). DNA was extracted from the digest with the QIAamp PowerFecal DNA Kit (Qiagen ...
-
bioRxiv - Plant Biology 2020Quote: ... citri adults and 20 mg of plant tissues was extracted using the DNeasy blood and tissue or DNeasy plant kits (Qiagen Inc, Valencia, CA), respectively ...
-
bioRxiv - Genetics 2022Quote: Total RNAs were extracted from cells using the RNeasy extraction kit and treated with RNase free DNAse set during 20 min (Qiagen, Valencia, CA, USA) or from blood using PaxGene blood RNA kit extraction (Preanalytix ...
-
bioRxiv - Microbiology 2022Quote: ... strain SPR117 was grown during 7 days in R2A broth at 20 °C and its genomic DNA (gDNA) was extracted using the Dneasy UltraClean Microbial Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: Approximately 20 mg of tail tissue was used to purify DNA or RNA with the QIAamp Fast DNA Tissue Kit (Qiagen, Valencia, CA, USA) or the RNeasy® mini Kit (Qiagen) ...
-
bioRxiv - Immunology 2021Quote: ... Following the manufacturer’s instructions RNA was reverse-transcribed in a 20 μl reaction volume (42°C, 30 min; 95°C, 5 min) using a QuantiTect Reverse Transcription Kit (Qiagen, Valencia, CA, USA). cDNA was then amplified using a SYBR Green I Master mix (Roche ...
-
bioRxiv - Biochemistry 2022Quote: ... The isolated RNA was air-dried after precipitation and taken up in 20 μl of RNase-free water followed by a subsequent sample purification using the RNeasy kit (Qiagen, Venlo, The Netherlands). Residual DNA contamination was removed from each sample using the Turbo DNA-free kit (Ambion ...
-
bioRxiv - Neuroscience 2023Quote: ... A sample of the resulting homogenate (10-20 mg) was used to assess total cellular RNA using the RNeasy® Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: ... the microbial DNAs were extracted directly from the 20 Strain Staggered Mix Genomic Material (ATCC MSA-1003) without size selection using a QIAamp DNA stool mini kit (Qiagen, Valencia, CA, USA). For the human gut microbiomes ...
-
bioRxiv - Molecular Biology 2020Quote: Total RNA was prepared from day one adult animals raised at 25°C or 20°C by using RNeasy® Mini Kit (Qiagen, Venlo, The Netherlands). endu-2(tm4977 ...
-
bioRxiv - Genomics 2020Quote: Genomic DNA was isolated from 20 female F1 pupae following a modified Qiagen Genomic Tip DNA Isolation kit (Qiagen Cat No. 10243 and 19060) protocol ...
-
bioRxiv - Genomics 2020Quote: ... The beads were then washed twice with 70% ethanol and resuspended in 50 μl sample elution buffer (Qiagen AE buffer with 0.1% Tween 20). After incubation at 20°C for 5 min ...
-
bioRxiv - Genomics 2021Quote: Genomic DNA was isolated from 20 inbred male pupae following a modified Qiagen Genomic Tip DNA Isolation kit (Qiagen Cat No. 10243 and 19060) protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... The lysate was cleared by centrifugation (35,000 x g, 1 h) and the supernatant was loaded onto a Ni-affinity chromatography column (Qiagen Ni-NTA Superflow, 20 mL) pre-equilibrated with Ni-NTA buffer ...
-
bioRxiv - Molecular Biology 2022Quote: Total RNA was prepared from synchronized day one adult animals raised at 20°C by using RNeasy® Mini Kit (Qiagen, Venlo, The Netherlands). Oligo-dT primer was used to synthesize cDNA by using Transcription First Strand cDNA synthesis kit (Roche ...
-
bioRxiv - Zoology 2023Quote: ... Then 25-50 mg of rehydrated tissue was cut into small pieces with a sterile disposable scalpel blade and transferred into individual 2 ml Eppendorf tubes containing 180 µL of ATL and 20 µL of proteinase K provided by the IndiSpin® QIAcube® HT Pathogen Kit (Qiagen, Courtaboeuf, France). Tubes were incubated between 6 and 12 h at 56°C until full proteolysis was achieved ...
-
bioRxiv - Genetics 2024Quote: The organs of Corti of postnatal day (P)4 mice were dissected and stored at -20°C in RNAprotect Tissue Reagent (RNAlater®) (QIAGEN, cat. no. 76106). Total RNA was extracted using the SPLIT RNA extraction kit (Lexogen ...
-
bioRxiv - Plant Biology 2023Quote: ... All soil samples were stored at –20°C until rhizosphere DNA was extracted using a QIAGEN DNeasy PowerSoil Kit (Qiagen, Inc., Valencia, CA, USA) following the manufacturer’s protocol.
-
bioRxiv - Immunology 2022Quote: ... and the resin was washed with one column volume of wash buffer NPI-20 (buffer composition can be found in the Qiagen Ni-NTA Superflow BioRobot Handbook) four times ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from homogenates after overnight incubation with 20 μl of proteinase K at 56°C using the QIAamp DNA Micro Kit (Qiagen, Hilden, Germany, cat. No. 56304) following the manufacturer’s protocol for tissue samples ...
-
bioRxiv - Neuroscience 2023Quote: DNA isolation and quantification was performed as previously described43,44,68 using a high molecular weight genomic DNA purification kit according to the manufacturer’s protocol (QIAGEN Genomic tip either 20/G or 100/G) and Quant-iT Picogreen dsDNA quantification ...
-
bioRxiv - Immunology 2023Quote: ... The remaining cells were pelleted by centrifugation (4000rpm, 30min, 4C) and stored at –20°C for bulk EEV extraction by endotoxin-free maxiprep (Qiagen EndoFree Maxi Kit, Cat. 12362).
-
bioRxiv - Genomics 2020Quote: ... ~20 ng genomic DNA was amplified using a whole-genome amplification (WGA) kit according to the manufacturer’s instructions (Qiagen REPLI-g Mini Kit, Qiagen, Valencia, CA). The REPLI-g Kit is developed based on multiple displacement amplification (MDA) ...
-
bioRxiv - Microbiology 2021Quote: ... whole ticks were bisected using a sterile 16-gauge needle and incubated in a lysis buffer (i.e., 180 μl Qiagen Buffer ATL and 20 μl proteinase K) at 56°C overnight ...
-
bioRxiv - Cell Biology 2024Quote: ... were incubated in a 50 µL reaction with or without triton-x (1% v/v) and with or without proteinase K (Qiagen #19131, final concentration 20 µg/mL) for 15 minutes at room temperature ...