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Citations for Qiagen :
551 - 600 of 1045 citations for Mouse IgG1 Isotype Control Antibody 15H6 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... The sample was incubated at room temperature for 5 min and 3.75μL (25 fmol final concentration) of the synthetic spike-in control Caenorhabditis elegans miRNA cel-miR-39 (Qiagen, Cat. No. 219610) was spiked into samples ...
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bioRxiv - Neuroscience 2022Quote: ... or a non-targeting scrambled control (Scr; sequence: 5’-TAACACGTCTATACGCCCA-3; Qiagen, Cat No.: 339137; 0.1 nmol in 2 μL PBS), using a 2 μL Hamilton syringe at a rate of 1 μL/min ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from GWSS and control samples (nGWSS = 79, ncontrol = 6) using a DNeasy PowerSoil DNA Isolation kit (Qiagen, Germany) with minor changes to the manufacturer’s protocol as follows ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from leaf samples of the control and treated (chilling, high light and chilling plus high light) plants using the RNeasy Plant Mini Kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from MCF10AER/vSrc cells (Control and treated with 10 nM D-Alanine for 4 h or 24 h) using RNeasy Kit (Qiagen, #74104) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Total DNA was isolated from the whale blow and technical control samples in one batch using a QIAamp DNA Mini Kit (QIAGEN, Germany). The primers used for sequencing the 16SrRNA V3 and V4 regions were 341F (5’-CCTACGGGNGGCWGCAG ...
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bioRxiv - Genomics 2023Quote: ... To then quality control the MNase digestions: 125 μL of each sample were cleaned-up using QIAquick PCR Purification Kit (QIAGEN, 28106) and assessed by agarose gel and Bioanalyzer ...
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bioRxiv - Genetics 2022Quote: ... and sex-matched controls were grown to 80-90% confluency in T75 flasks before total RNA extraction via RNeasy Plus Mini Kit (Qiagen, 74136). Whole blood samples were collected and purified using MagMAX for Stabilized Blood Tubes RNA Isolation Kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... Quality control of tissue blocks was performed by extracting RNA from FFPE samples using the Qiagen RNeasy FFPE Kit (Qiagen, 73504), followed by the assessment on the Agilent 2100 Bioanalyzer using the RNA 6000 Pico Kit (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... for 24 hours and transfected with 100 nM LysTTT-5’tRF mimic or negative control oligonucleotide (IDT, Syntezza Bioscience, see Supp. Materials and Methods for sequences) using HiPerFect transfection reagent (Qiagen, 301705). After incubation for an additional 24 hours in the same OA-rich medium cells were harvested for RNA and protein or fixed for triglyceride quantification.
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bioRxiv - Cancer Biology 2023Quote: ... MiRs (61 plus 3 controls) were quantified on a custom PCR plate using the miRCURY LNA miR SYBR Green PCR Handbook (Qiagen, Germany). PCR (40 cycles ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 100 mg leaf samples of stressed and unstressed plants [three replicates of each control (WT) and transgenic (L1) plants] using the RNeasy Plant Mini kit (Qiagen, Germany). Sequentially first strand cDNA ...
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bioRxiv - Neuroscience 2024Quote: The fresh-frozen brain tissue from control and epileptic animals was homogenized with a handheld rotor-stator homogenizer (QIAGEN, TissueRuptor II) in a lysis buffer that contains 0.5% Triton X-100 ...
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bioRxiv - Cancer Biology 2023Quote: RT PCR: Total RNA was eluted from knockdown and control cells using the RNeasy® Plus mini kit (Qiagen, Ref # 74134). 2 µg of total RNA was reverse transcribed using High Capacity cDNA Reverse Transcription Kit (AppliedBiosystems ...
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bioRxiv - Cell Biology 2022Quote: Mouse Plin2 homology arms were obtained by extracting DNA from mouse primary mouse NSPCs from the SVZ using DNeasy Blood & Tissue Kit (#69506, Qiagen). The sequences covering the sgRNA target site were extracted from the genomic DNA using PCR ...
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bioRxiv - Genomics 2022Quote: ... The DNA from mouse bladder tumors and matched germline DNA from mouse tails were extracted using the DNeasy Blood Tissue kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with primary antibody (Penta·His Antibody, QIAGEN®, 1:2000 dilution) in 1% bovine serum albumin in PBST for 2h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... for mouse cells or the RNeasy kit (Qiagen) for human cells ...
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bioRxiv - Cell Biology 2023Quote: ... or siRNA for Anxa2 (Mouse, Qiagen, Mm_Anxa2_3, SI00167496).
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bioRxiv - Biochemistry 2023Quote: Mouse EL4 genomic DNA was isolated by QIAGEN Blood & Cell Culture DNA Kit (QIAGEN ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse tissues were homogenized using TissueLyser II (Qiagen) and stainless-steel beads (5 mm ...
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bioRxiv - Biophysics 2021Quote: ... were transfected with 50 nM of si-AKR1Cs or si-scramble as control (sequences are provided in Table S1) using HiPerfect transfection reagent (Qiagen, Valencia, CA). After 24 or 48 h transfection ...
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bioRxiv - Cell Biology 2022Quote: ... DNA samples were extracted from PKS skin fibroblasts and normal control skin fibroblasts using the Puregene DNA isolation kit (Qiagen, Valencia, CA) with modified protocol ...
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bioRxiv - Microbiology 2019Quote: ... The relative abundance of different bacterial species was determined by comparing the Ct value of each bacterial species with the Ct values of same bacterial species generated from the healthy controls using the Baid 1407 intestinal infections microbial profiling data analysis software (Qiagen, Hilden Germany).
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bioRxiv - Cell Biology 2021Quote: ... the cells were co-transfected with the 9 × GCCG-reporter construct and the renilla-luciferase control vector using SuperFect (Qiagen, Valencia, CA) for 24 h ...
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bioRxiv - Cancer Biology 2020Quote: ... of hsa-miR-145-5p miRCURY LNA miRNA Power Inhibitor (Exiqon, YI04102423-DCA) or miRCURY LNA miRNA Power Inhibitor Negative Control A (Qiagen, YI00199006-DDA) at a final concentration of 50 nM and confirmed by RT-qPCR with the miRCURY LNA™ Universal RT microRNA PCR system (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... Samples and the DNA extraction negative control (1 ml PBS) were processed for DNA extraction using the QIAamp DNA Microbiome kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol (Depletion of Host DNA protocol).
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bioRxiv - Cancer Biology 2021Quote: The LNA GapmeR oligonucleotides specifically targeting SAMMSON and the LNA non-targeting control (NTC) GapmeR were purchased from Qiagen (catalog number 339517).
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bioRxiv - Genomics 2022Quote: RNA from serum samples of severe ROP and no/mild ROP control was extracted by the QIAamp RNA Blood Mini kit method (Qiagen, Hilden, Germany). The cDNA was prepared using the iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Pathology 2020Quote: ... calcitrans and known vertebrate whole blood samples as positive controls were isolated using DNeasy Blood and Tissue kit (Cat No./ID: 69504, Qiagen, Hilden, Germany). Fed flies were selected by observing engorged abdomens and then the guts of blood-fed flies were crushed and homogenized individually using ten 2-mm yttria-stabilised zirconia beads in 400 μL of cold homogenization phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... The matched control DNA was also isolated from blood of the same patients using Gentra Puregene Blood Kit (Qiagen, Cat. No 158467). To generate short-fragment DNA libraries ...
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bioRxiv - Bioengineering 2022Quote: ... Following seeding MSCs were transfected with 20nM of either pBAE:miR-29b (miR-29b) complexes or pBAE:siRNA (Scramble) complexes (Qiagen, AllStars Negative Control siRNA) or cultured in standard control medium (control ...
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bioRxiv - Genomics 2022Quote: Lists of genes that were either significantly overexpressed or underexpressed compared with controls were analyzed using the Ingenuity Pathway Analysis (IPA) core pathway (Qiagen Ingenuity Systems) to identify significantly affected canonical pathways ...
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bioRxiv - Genetics 2022Quote: ... DNA from both the patients and healthy controls’ buffy coat was isolated using QIAamp DNA blood mini kit (Qiagen Inc; Germanton, MD). The DNA samples were genotyped using Illumina’s Infinium Immuno array-24 v2 chip at UW-Madison’s Gene Expression Center (GEC) ...
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bioRxiv - Neuroscience 2022Quote: Total forebrain RNA (10 μg) was isolated from three pairs of littermate matched AtrxFoxG1Cre and control P17 mice using the RNeasy Mini kit (Qiagen Cat# 74104). cRNA was generated and hybridized to an Affymetrix Mouse Genome 430 2.0 Array at the London Regional Genomics Center (London ...
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bioRxiv - Neuroscience 2022Quote: RNA was extracted from seven-week PSEN1 fAD- and isogenic control hiPSC derived neurons using the RNeasy® Plus Mini Kit (Qiagen, 74134), according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA from the control human TSCs as well as GATA2-KD and GATA3-KD human TSCs were isolated using RNeasy Mini Kit (Qiagen, Germantown, MD) per the manufacturer’s protocol with on-column deoxyribonuclease digestion ...
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bioRxiv - Cancer Biology 2023Quote: 1×104 SiHa or 1×103 Hela cells/6-well were seeded and transfected with pSG5-miR-142-5p expression vector or pSG5 empty vector as a control using PolyFect Transfection Reagent (Qiagen, Hilden, Germany). After 48 h ...
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bioRxiv - Plant Biology 2023Quote: ... and pXVE::YFP-cPMEI8 plants treated with control MS and 5 µM estradiol for 24 h using the RNeasy Plant Kit (QIAGEN, Hilden, Germany). For RNA isolation from meristematic and elongation zones ...
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bioRxiv - Cancer Biology 2023Quote: ... BRD4-S (targeting short variant 3’-UTR region; Cat#: SI05044865) and AllStars Negative control (Cat. #: SI03650318) were purchased from QIAGEN (MD, USA). ITGA4 siRNA (Cat ...
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bioRxiv - Microbiology 2023Quote: ... in-house mock community (25 diverse Gram-positive and Gram-negative bacteria and fungi) and a negative reagent control (nuclease-free water, Qiagen, Hilden, Germany) were included ...
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bioRxiv - Cell Biology 2023Quote: ... gDNA from cells selected with BFA or FLI-06 and unselected control cells were extracted using the QIAamp DNA Blood Maxi Kit (Qiagen, cat.no. 51194). The integrated sgRNA cassette was amplified in a first PCR ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from the SBMA OL cell model and the control cell model using the RNeasy Mini Kit (Qiagen, Hilden, Germany). The quality and quantity of the RNA obtained were checked using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and colon (n = 35 each) and negative blank DNA extraction kit controls using the DNeasy PowerLyzer Powersoil kit (Qiagen, Germantown, MD, USA), with minor modifications to the manufacturer’s protocol as previously described51–53 ...
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bioRxiv - Immunology 2020Quote: ... and detection was performed using anti-His antibody (Penta-His Antibody, #34660, Qiagen) followed by donkey-anti-mouse antibody labeled with AlexaFluor647 (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: DNA contents in native/control kidneys (n = 4) and decellularized kidneys (n = 4) were measured using a Qiagen DNeasy Kit (Qiagen, Valencia, CA, USA). The tissues were initially minced and stored overnight at −80°C ...
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bioRxiv - Immunology 2021Quote: Total RNA was prepared after stimulation or that of untreated controls with the RNeasy Mini/ Plus Micro Kit (Qiagen, UK cat# 74106/ 74034). Stimulated cells were lysed in buffer RLT (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted from soil subsamples in triplicate and with negative control using the DNeasy PowerSoil DNA isolation kit (Qiagen, Germantown, MD, USA), according to the manufacturer’s instructions with one minor modification ...
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bioRxiv - Plant Biology 2023Quote: ... RT-PCR reactions for each primer set were performed on RNA (50-75 ng/ul) from at least two biological replicates of the mutant allele and control (QIAGEN OneStep RT-PCR Kit, QIAGEN Inc., Cat # 210212). The bounds of where transcriptional termination and initiation occurs within Mu for each mutant allele was determined by presence of amplification from gDNA and absence of amplification from cDNA ...
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bioRxiv - Physiology 2023Quote: DNA content in native/control kidneys (n = 4) and decellularized kidneys (n = 4) were measured using a Qiagen DNeasy Kit (Qiagen, Valencia, CA, USA). The tissues were initially minced and stored overnight at −80°C ...