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Citations for Qiagen :
451 - 500 of 1045 citations for Mouse IgG1 Isotype Control Antibody 15H6 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The transfection of Wls-shRNA and pLCN-Wnt3a-HA and controls was performed by using HiPerFect transfection reagent (#301704, Qiagen) according to the manual ...
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bioRxiv - Molecular Biology 2020Quote: ... after adding 1.0 x 10^8 copies/μL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted from MTX-resistant HT-29 cells and control samples using the Gentra Puregene Cell kit (Qiagen) and the library for PacBio ...
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bioRxiv - Genomics 2019Quote: Total RNAs from the knockdown (shCTCF#1 and shCTCF#2) and control (shLuc) cells were extracted using miRNeasy Micro Kit (QIAGEN). cDNA was synthesized by using oligo (dT)20 and SuperScript III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... SLE mice were retro-orbitally injected with 10 mg/kg of LNA-miR-22-3p (LNA-22) or scrambled control (LNA-Scr) (miRCURY LNA Inhibitor probe in-vivo, Qiagen) every 2 weeks beginning at 10-12 weeks of age ...
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bioRxiv - Neuroscience 2019Quote: Total cellular RNA from HOTCs on multiple pooled plates was harvested from treated or control HOTCs using the RNeasy RNA Isolation Kit (Qiagen). RNA quality and quantity was analyzed with spectrophotometry and gel electrophoresis.
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bioRxiv - Genetics 2019Quote: ... Genomic DNA and RNA were extracted from peripheral blood leukocytes from patient 6003 and from a non-HHT control individual using the Gentra PureGene Blood Kit (Qiagen) and TRIzol Reagent (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... siRNAs used were: AllStars Negative control siRNA (cat# 1027281) and si-Rab13 #8 (cat# SI02662702; target sequence: 5’-ATGGTCTTTCTTGGTATTAAA-3’) from Qiagen.
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bioRxiv - Genomics 2020Quote: ... we prepared a negative (FU = fully unmethylated) control sample from differentiated HepaRG mtDNA by performing whole genome amplification using a repliG kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... 5.6 x 105 cells were seeded in 4 ml DMEM in a 6-cm culture plate and transfected with targeting or control siRNA (Table 4) (from Qiagen) to a final concentration of 20 nM for each siRNA used ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were co-transfected with the reporter construct and the renilla-luciferase control vector using SuperFect (Qiagen, Valencia, CA) for 24 hr ...
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bioRxiv - Microbiology 2021Quote: ... Quality control and de novo assembly of the reads were done using default parameters in CLC Genomics workbench 7 (Qiagen). Whole-genome alignment was performed using Mugsy v1.2.3 (55 ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from 4 g of soil from the identified segment of three 13C soil cores and from the 12C-control for each substrate/sample site combination using the DNeasy PowerMax Soil Kit (Qiagen). DNA was quantified fluorometrically using the Qubit dsDNA Broad-Range assay (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA from CRISPR-on HEK293T cells and control cells were purified using a AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Then ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293 cells were transfected with plasmids (WT mGlu1, the mGlu1 mutants, or the control vector) using SuperFect transfection reagent (Qiagen) or Viafect (promega ...
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bioRxiv - Cell Biology 2021Quote: siRNAs against RAB5A(S100301588), RAB5B (S102662800), RAB5C(S102663073), RAB8A (SI02662254), RAB8B(SI02662261), RAB10 (SI00301546, SI03037076) and non-targeting control were purchased from Qiagen AS (FlexiTube siRNA) ...
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bioRxiv - Cell Biology 2020Quote: ... p21 or p53 as well as a scrambled control siRNA were purchased from Dharmacon and employed at a concentration of 20nM using HiPerFect transfection reagent (Qiagen) and OptiMEM (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... Our panel of 88 target genes was prepared on a 96-well plate format with housekeeping genes and controls (RT2 PCR array, Qiagen). Expression was quantified on an ABI7500 real-time PCR machine (Applied Biosystems ...
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bioRxiv - Developmental Biology 2021Quote: ... total RNA was extracted from control and INPP5ED477N/D477N organoids (n=3 samples per genotype) using an RNeasy Plus Micro Kit (Qiagen) and reverse transcribed using Superscript™ IV VILO™ Master ezDNase enzyme (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from control and DAC treated U2OS cells growing on the 6-well plates using miRNAeasy kit (Qiagen) according to the manufacturer’s protocol and eluted in 50 μL RNAse-free water ...
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bioRxiv - Molecular Biology 2019Quote: Genomic DNA was isolated from control and DAC treated U2OS cells growing on the 6-well plates using QIAamp DNA Mini Kit (Qiagen), and bisulfite-converted using MethylEdge™ Bisulfite Conversion System (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was generated from CSF isolated RNA and a control RNA sample spiked with miR-39 (1 × 108 copies/ul) using the miScript HiSpec kit (Qiagen); dilutions were created from the control cDNA sample spiked with miR-39 (1 ×106-1×103 copies ...
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bioRxiv - Neuroscience 2021Quote: A total of 8 frozen brain samples (five controls and three infected with ZIKV) were processed in TissueLyser® (Qiagen) for 30 seconds at 30Hz for cell disruption ...
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bioRxiv - Neuroscience 2021Quote: ... total RNA was isolated from laser-microdissected control and mutant samples (n = 4) using the RNeasy Micro Plus Kit (Qiagen). The isolated RNA was checked for purity and integrity using Nanodrop spectrophotometer and TapeStation (Agilent) ...
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bioRxiv - Immunology 2020Quote: Peripheral blood monocyte subpopulations were sorted from Aldara treated mice or untreated controls (Figure S3A) and RNA was isolated using RNeasy micro kit (Qiagen). Libraries were constructed from total RNA with the ‘SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian’ (Takara/Clontech ...
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bioRxiv - Genetics 2022Quote: Total RNA was extracted from the stomach and pyloric caeca tissue samples stored at -80 °C (n = 4 for control and fly larvae, n = 5 for shrimp shell) using the RNeasy Plus Universal Kit (QIAGEN). RNA quality was assessed using a 2100 Bioanalyzer with the RNA 6000 nano kit (Agilent ...
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bioRxiv - Genetics 2022Quote: ... Subsequent purification of FUSE and control samples was performed in silica columns using the standard protocol as recommended by the manufacturer (Qiagen DNeasy Plant Kit Qiagen Inc ...
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bioRxiv - Neuroscience 2022Quote: sMN total RNA from DHMN1 patient (n = 3) and controls (n = 3) was isolated using the RNeasy mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was extracted and purified from pooled scrambled control and genotyped cldn7b F0 knockout larvae (n=50 per group) at 7dpf using the RNeasy Plus Micro Kit (QIAGEN), followed by cDNA synthesis with the SuperScript IV VILO Master Mix Kit (ThermoFisher) ...
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bioRxiv - Cell Biology 2019Quote: ... human primary monocytes were transfected with 200 nM of ON-TARGETplus SMARTpool siRNA targeting Siglec-1 (Horizon Discovery) or non-targeting siRNA (control) using HiPerfect transfection system (Qiagen). Four hours post-transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were transfected with 500 ng of pGL3- DARPP-32 plasmids and 50 ng of pRL-SV40 plasmids (renilla; internal reference control) using Polyfect transfection reagent (Qiagen). Lastly ...
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bioRxiv - Pathology 2020Quote: ... and the RT product was used for detection of miR-574-5p/3p and control RNA Snord68 using the miScript Primer Assay (Qiagen).
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bioRxiv - Immunology 2020Quote: RNA was extracted from isolated neutrophils after the 1 and 4 hour EC challenges as well untreated controls using the miRNeasy Micro kit from Qiagen and libraries were generated using KAPA PolyA enrichment mRNA library prep ...
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bioRxiv - Genetics 2019Quote: RNA was extracted from skin fibroblasts of individual II.4 and a healthy unrelated control using Rneasy RNA kit (Qiagen) then we performed reverse transcription using the iScriptTM cDNA Synthesis Kit (BioRad ...
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bioRxiv - Cell Biology 2019Quote: ... GGA family member and interactor targeting siRNAs (Oligo IDs indicated in Fig. S1A) and control siRNA were purchased from Qiagen. Before plating MDA-MB-231 cells on CSMA ...
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bioRxiv - Genomics 2019Quote: ... RNA was extracted from infected (n = 4) and control (n = 4) bAM samples using the RNeasy Plus Mini Kit (Qiagen) as previously described [91] ...
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bioRxiv - Biochemistry 2021Quote: ... and 675 μL PCR products for control cells and 320 μL were applied to the 2% agarose gel for purification using QIAquick Gel Extraction Kit (QIAGEN). The PCR products of control and sort2 were concentrated by using Amicon Ultra-0.5 10K (UFC501096 ...
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bioRxiv - Cancer Biology 2021Quote: ... The LNA gapmers for lnc-FLii-1 and lnc-LSAMP-3 were designed and purchased along with negative control LNA from Qiagen. For transfection ...
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... H460 and H2023 cells 48 hrs following transfection with control siRNA or NRF2 siRNA (cat. no. HSS107128 and HSS107130) using the RNeasy Mini Kit (Qiagen). Cells treated with the control siRNA were analyzed in biological duplicates ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from melan-uw cells stably expressing either F374 or L374 variants of HA-SLC45A2 or HA-SLC35D3 as a control using the RNeasy Plus Kit (Qiagen), and 3 µg was reverse transcribed (RT ...
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bioRxiv - Cell Biology 2020Quote: ... 20 24 hpf zebrafish embryos injected with Grnb-MO or uninjected controls were collected and RNA was isolated with RNeasy (Qiagen), cDNA generated with qScript Supermix (Quanta BioSciences ...
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from one wild-type control and three patient cell lines using DNeasy Blood and Tissue kit (Qiagen). Specific primers were designed to cover approximately 500 base pairs around the mutations and the regions were PCR-amplified using Qiagen Multiplex PCR kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: THP-1 human monocytes or mature primary human peripheral blood derived macrophages were transfected with control (D-001810-10-05, Dharmacon) or SP140 (L-016508-00-0005, Dharmacon) SMARTpool siRNA (100nM) using HiPerfect reagent (Qiagen) or RNAiMAX (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... DNA of FACS sorted iTregs and control cells from female and male donors was isolated using the DNeasy Blood and Tissue Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Genes from human and mouse datasets were identified to be differentially expressed from each in comparison to a control group using either Ingenuity Pathway Analysis software (Qiagen), or Geo2R73 ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from control (n = 2) and olaparib-treated (n = 2) GTFB-PDX1009 ascites using the RNeasy Plus Mini kit (Qiagen). RNA quality was confirmed using an Agilent TapeStation and all RNA used for library preparation had a RIN>9 ...
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bioRxiv - Cancer Biology 2022Quote: ... EB’s and Bl-CFC obtained from either ENU-treated or not treated CML iPSC as well as from control iPSC were used for DNA extraction using standard methods with DNeasy Kit (Qiagen).
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bioRxiv - Genetics 2023Quote: All cDNA samples were pooled into two groups corresponding to cases (alcohol drinkers) and controls (non-drinkers) and then assayed using the Serum/Plasma Focus PCR panel (Qiagen). This commercially available microarray platform contains 179 LNA miRNA primer sets of miRNAs that have been commonly found in human plasma ...
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bioRxiv - Molecular Biology 2023Quote: Primary monocytes were cultured in 24-well plates and then transfected with control non-targeting siRNA (Dharmacon) or siRNA targeting ERN1 (Dharmacon) using HiPerFect reagent (QIAGEN) following the instructions provided by the manufacturer ...
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bioRxiv - Plant Biology 2023Quote: ... RT-PCR reactions for each primer set were performed on RNA (50-75 ng/ul) from at least two biological replicates of the mutant allele and control (QIAGEN OneStep RT-PCR Kit ...