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Citations for Qiagen :
551 - 600 of 1140 citations for IL 5 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... then subsequently homogenised four times with a 5 mm diameter stainless steel bead (Qiagen) for 3 min at 50 Hz using a TissueLyser LT (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... were isolated using the “miRNeasy Mini Kit” (cell numbers ≥ 5 × 105, Qiagen, Cat# 217004). FACS-isolated FO or MZ B cells were directly sorted into the Qiazol Lysis Reagent (700 μl final volume) ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from pools of 5 females using an RNeasy Mini kit (Qiagen) by first homogenizing them using a plastic pestle in 600 µL of lysis buffer in a 1.5 mL microfuge tube ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was applied to a 5 ml nickel-nitrilotriacetic acid column (Qiagen, Valencia, CA) that had been equilibrated with buffer A at a rate of 1 ml/min ...
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bioRxiv - Pathology 2022Quote: ... with 2 x 5 mm stainless steel beads using a TissueLyser (Qiagen, Germantown, MD) for 3 minutes at 25 r/s for 2 cycles ...
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bioRxiv - Plant Biology 2022Quote: ... The suspension was applied to a column (5 ml polypropylene column, Qiagen, Hilden, Germany) with subsequent washing (5 column volumes ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from 5 million sorted bacteria using DNeasy Blood & Tissue (Qiagen, 69504) following manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Each sample was analysed in duplicate ...
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bioRxiv - Pathology 2023Quote: ... with 2 x 5 mm stainless steel beads using a TissueLyser (Qiagen, Germantown, MD) for 3 minutes at 25 r/s for 2 cycles ...
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bioRxiv - Molecular Biology 2023Quote: Approximately 5 mg of frozen liver was homogenized in 1 ml RLT buffer (Qiagen) using a BeadBeater (BioSpec ...
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bioRxiv - Microbiology 2023Quote: ... 5 ml samples of each culture were stabilized with RNA protect Cell Reagent (Qiagen) and subsequently collected by centrifugation at 5 000 x g for 10 min at 4◦C ...
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bioRxiv - Cell Biology 2023Quote: ... The control cells were replated after 5 days of AllStars negative-control siRNA (QIAGEN) treatment ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was batch bound to 5 mL of Ni-NTA Agarose (Qiagen, Cat# 30230) resin for 90 minutes stirring in a beaker at 4ºC ...
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bioRxiv - Immunology 2023Quote: Total mRNA was isolated from 5×105 cells using a kit (RNeasy kit, QIAGEN) and reverse-transcribed using oligo(dT)20 primer (SuperscriptIII ...
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bioRxiv - Biochemistry 2024Quote: ... The solution was applied to a column of 5 ml Ni-NTA agarose (Qiagen) equilibrated in Lysis-Wash buffer and the flow-through collected ...
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bioRxiv - Cancer Biology 2024Quote: ... 1X106 cells were mixed with 5 μl of 20 μM siRNAs (GeneSolution siRNA, Qiagen) and 100 μl of Ingenio electroporation solution (Mirus ...
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bioRxiv - Bioengineering 2020Quote: ... A Qiagen RT2 SYBR Green master mix with validated qPCR human primers (for HUVECs and BeWo) or mouse primers (for N27 cells) from Qiagen (Frederick) were used to determine relative magnitudes of gene-expression levels using RT-PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... The human U6 primer used in this experiment was included in the miScript Primer assay kit (Qiagen Inc., Germantown, MD, #218300).
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bioRxiv - Molecular Biology 2022Quote: ... FAF1 (Uniprot identifier Q9UNN5-1) and UBXN7 (Uniprot identifier O94888-1) were amplified from XpressRef Universal Total human RNA (QIAGEN, 338112) by RT-PCR (TaKaRa ...
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bioRxiv - Developmental Biology 2021Quote: ... The expression levels of the mRNAs were quantified using Human Embryonic Stem Cell RT2 Profile™ PCR Array (Cat. PAHS-081, Qiagen) and RT2 SYBR Green qPCR Master Mix (Cat ...
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bioRxiv - Developmental Biology 2021Quote: RNA was isolated from a 23 week human fetal heart using Trizol-based dissociation followed by the RNEasy Mini Kit (Qiagen #74104). cDNA was created from this RNA using the iScript Reverse Transcription Supermix (Bio-Rad #1708840) ...
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bioRxiv - Systems Biology 2022Quote: DNA was isolated from human buffy coat samples at the Crimson Core facility (Mass General Brigham) using the QIAamp DNA Blood Mini Kit (Qiagen 69504) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Reference plasmids were serially diluted in either PBS buffer or heat-inactivated normal human serum (NHS) and re-extracted using the QIAamp® DNA Blood Mini Kit (Qiagen) per recommended protocol for serum ...
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bioRxiv - Cancer Biology 2020Quote: Flag-tagged NF1-GRD (aminoacids 1131-1534) was amplified by PCR from human cortical tissue (epilepsy patient) and first cloned in the pDRIVE vector (Qiagen #231124). Primers are listed in Supplementary Table 5 ...
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bioRxiv - Immunology 2021Quote: ... and ImmunoCult™ Human Treg Differentiation Supplement (StemCell TechnoRNA was isolated at set time points after activation using the RNeasy Mini (Qiagen). cDNA was generated using the High-Capacity RNA-to-cDNA Kit (ThermoFisher) ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted from human cell lines of WI-38 and MED13LS1497F using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) according to manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... Exosomal miRNAs were profiled using Human Serum/Plasma miRCURY LNA miRNA PCR array (Qiagen, #YAHS-106Y, Plate Format: 2 × 96-well).
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bioRxiv - Microbiology 2023Quote: RNA was extracted from a SARS-CoV-2 Omicron BA.1 clinical isolate (SARS-CoV-2/human/USA/CA-CDC-4358237-001/2021) (GenBank: OM264909.1) using the QIAamp Viral RNA kit (Qiagen, Hilden, Germany). The complete wild-type Omicron genome was then reverse transcribed via RT-PCR with SuperScript™ IV First-Strand Synthesis System (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... 1 µg of RNA isolated from human islets or 10 µL of exosomal RNA was reverse transcribed using the miRScript II kit (Qiagen, Germany), following manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... RT PCR was performed on a Roche LightCycler 96 using the The RT² Profiler™ PCR Array Human Cell Death PathwayFinder (PAHS-212Z, Qiagen)w ...
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bioRxiv - Neuroscience 2023Quote: ... The association of the differentially expressed proteins with specifically dysregulated regulatory/metabolic networks in OT human samples was analyzed using QIAGEN’s Ingenuity Pathway Analysis (IPA; QIAGEN Redwood City). This software calculates significance values (p-values ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from the proteinase K-digested tail tips of mouse or peripheral blood mononuclear cell (PBMC) of human donors using DNeasy blood tissue kit (Qiagen, #69506). 25 ng of genomic DNA was subjected to bisulfite conversion using a commercial kit (Zymo Research ...
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bioRxiv - Physiology 2019Quote: ... DNA was extracted from 5 µL of RBCs using the Gentra Puregene Tissue Kit (Qiagen) and kept frozen at −80°C until analysis ...
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bioRxiv - Synthetic Biology 2020Quote: ... and homogenized with 5 mm stainless steel beads on a Tissue Lyzer II (Qiagen 85300) at 30 Hz for 30 minutes ...
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bioRxiv - Molecular Biology 2019Quote: 5 µg of RNA of was cleaned up using using RNeasy mini prep column (Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... for 2 hours after which samples were treated with 5 μL Proteinase K (Qiagen 19131) overnight ...
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Persistent DNA damage rewires lipid metabolism and promotes histone hyperacetylation via MYS-1/Tip60bioRxiv - Cell Biology 2021Quote: ... The worms were homogenized with a 5□mm steel bead using a TissueLyser II (Qiagen) for 2□×□2.5□min at a frequency of 30 times/sec ...
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bioRxiv - Neuroscience 2020Quote: ... Powdered tissue (∼5 mg) was dissolved in lysis buffer provided by RNeasy micro kit (Qiagen), supplemented with 1% (v/v ...
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bioRxiv - Synthetic Biology 2021Quote: ... for 5 mins and then neutralized by addition of Qiagen N3 neutralization buffer (Qiagen, 19053). Subsequently ...
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bioRxiv - Physiology 2022Quote: ... Samples were homogenized using a TissueLyser II device (Qiagen; 5 min at 30 pulses/second) in 425 μL water ...
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bioRxiv - Bioengineering 2022Quote: ... 350 µL of NucleoSpin RNA extraction buffer along with 4-5 ceramic beads (Qiagen 13113) were added 14into the tube ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated from 5 or 6 Transwells using the RNeasy Micro Plus kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... BEAS-2B and MRC-5 (2×106 cells/well) using the RNeasy Mini Kit (Qiagen) with DNase I treatment to eliminate DNA contaminants as previously described18 ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from 5 × 106 cells using Qiagen DNeasy Blood & Tissue Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... phagocytosed by sorting cells into a 96 well plate containing 5 ul of RLT (Qiagen) + 1%β-Mercaptoethanol (Sigma).
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bioRxiv - Molecular Biology 2020Quote: ... whole worms were homogenized with a 5 mm steel bead using a TissueLyser II (QIAGEN) for 5 min at frequency of 30 times/second ...
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bioRxiv - Biochemistry 2020Quote: ... the eluted fraction was subsequently passed through a 5 ml Ni-NTA Superflow cartridge (Qiagen). The column was washed with 10 CV Ni-NTA Wash Buffer 1 (PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate was then batch bound to 5 mL of Ni-NTA Agarose (Qiagen, Cat# 30230) resin at 4°C for 1 hour in a beaker set on a stir plate ...
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bioRxiv - Biochemistry 2021Quote: ... The protein solution was subsequently applied to a 5 ml Ni-NTA Superflow cartridge (Qiagen) which was washed with dialysis buffer supplemented with increasing concentrations of imidazole (10-250 mM) ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was loaded onto gravity column containing 5 ml Ni-NTA affinity resin (Qiagen) pre-equilibrated and washed with 30 ml of Buffer A then eluted with Buffer A containing 300mM imidazole ...