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Citations for Qiagen :
551 - 600 of 1604 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: APOB expression determined by first isolating total RNA from Hep3B cells grown in 6-well plates using the RNeasy Mini Kit (Qiagen, #74104). Real-time qPCR was performed using the TaqManTM RNA-to-CtTM 1-Step Kit (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: RNA was extracted from iPSCs plated for 48 h on a Matrigel-coated 6 WP with the RNAeasy Micro Kit (Qiagen #74004) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from ripe pepper pericarp of RJ107(6)A3 (Maquilan et al., 2020) using RNeasy plant mini kit (Qiagen, Germany). First-strand cDNA was synthesized using SuperScript®III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Immunology 2024Quote: Cells were seeded (3 x 105) in 6-well plates and lysed using RLT buffer for RNA isolation (RNeasy Mini kit, Qiagen, #74106). Each sample was performed in triplicate ...
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bioRxiv - Neuroscience 2024Quote: ... and microglia (treated for 6 hours) treated with or without 1 µM CDDO-Me with a RNeasy Mini Kit (Cat. number: 74106; QIAGEN, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Cells were allowed to reach 80% confluency in a 6-well plate before total RNA was extracted using RNeasy kit (QIAGEN #74106) as per manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: ... plasmids were prepared from 5 ml overnight culture using Miniprep kits (Qiagen) and the plasmids were sequenced using M13F-M13R primers (Table S4).
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bioRxiv - Cell Biology 2020Quote: ... RNA was extracted from 5 million cells with RNeasy mini Kit (Qiagen) following the manufacturer’s instructions and with DNase treatment on column ...
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bioRxiv - Immunology 2020Quote: ... The cell pellet was resuspended in 5 ml RBC lysis buffer (Qiagen), incubated at room temperature for 5 minutes and then washed twice with 45 ml RPMI ...
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bioRxiv - Molecular Biology 2020Quote: Cells were collected in 5 µl cold RLT Plus buffer (Qiagen, 1053393) containing 1U/µl RNAse inhibitor (Ambion ...
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bioRxiv - Genomics 2020Quote: ... 5 pM of each primer and 2.0 U HotStar Taq polymerase (Qiagen) in a 25 µl volume or 1X Phusion U Hotstart (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were loaded onto a 5 mL Ni-NTA Superflow Cartridges (Qiagen) equilibrated with Buffer B (25 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: The siRNA sequence (5’-GAGUAGAACUAGAAUGUGA-3’) targeting Hec1 was synthesized by Qiagen. The ON-TARGETplus SMARTpool siRNA sequences (5’-GAACGAGUAACCACAAUUA-3’) ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed with lysis buffer supplemented with 25 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen). The column was washed in two steps with lysis buffer supplemented with 25 mM and 100 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was incubated with 5 ml of Ni-NTA resin (Qiagen) for 2h ...
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bioRxiv - Microbiology 2021Quote: ... and homogenized using 5 mm stainless-steel beads and a TissueLyser (Qiagen). Virus titers in serum and organ homogenates were determined by plaque assay using L929 cells [86] ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 µg RNA was DNase treated using RNase-Free DNase Set (Qiagen). 1 µg of DNase treated RNA was then taken for cDNA synthesis using the Protoscript I first strand cDNA synthesis kit (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl RNA was tested with the Rotor-GeneTM probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleavage reaction was run over a 5 mL Streptactin column (Qiagen) to remove any uncleaved HELB and free StrepII peptide and the cleaved HELB-containing flow-through collected ...
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bioRxiv - Immunology 2020Quote: Total RNA from 5×106 PBMCs was isolated using RNeasy kits (Qiagen). First-strand cDNA was generated from total RNA using SuperScript RT IV (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μg RNA was DNase treated using RNase-Free DNase Set (Qiagen) and 1 μg of DNase treated RNA was then taken for cDNA synthesis using the Protoscript I first strand cDNA synthesis kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected using siRNAs targeting MAD2 sequence 5′-AGAUGGAUAUAUGCCACGCTT-3′ (Qiagen), pools of siRNAs targeting the TP53 sequence 5’-GAAAUUUGCGUGUGGAGUA-3’ ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue homogenization was performed with stainless steel beads of 5 mm (Qiagen) and TissueLyser LT (Qiagen) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... with two 5 mm stainless beads using a TissueLyser (Qiagen, CA, USA) at 50 Hz for 4 min ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed with lysis buffer supplemented with 25 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen). The column was washed in two steps with lysis buffer supplemented with 25 and 100 mM imidazole ...
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bioRxiv - Genomics 2021Quote: ... The scramble-miR miRCURY LNA Detection probe (5’-GTGTAACACGTCTATACGCCCA-3’, YD00699004, QIAGEN) was used as a negative control ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleared lysate was loaded on a 5 mL Ni-NTA column (Qiagen) equilibrated with buffer C ...
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bioRxiv - Genetics 2020Quote: ... The 3’ and 5’ – DIG labeled miRCURY LNA miRNA Detection probes (Qiagen, negative control Scramble-miR cat ...
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bioRxiv - Molecular Biology 2021Quote: ... Worms were homogenised with a 5 mm diameter stainless steel bead (Qiagen) for 4 min at 50 Hz in 100 µl of 0.15 M McIlvaine buffer pH 4.6 [64] with EDTA-free protease inhibitors (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was isolated from 5×106 cells using the RNeasy minikit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... the reaction tubes were added 5 μL of PureGene Proteinase K (Qiagen) and incubated for additional 30 minutes at 50°C ...
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bioRxiv - Microbiology 2022Quote: ... then 5 µl of RNAse A (10 mg/ml; Qiagen, Hilden, Germany) was added and the sample was incubated for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... a proteinase K reaction solution containing 5 μL of PKD buffer (QIAGEN) and 0.31 μL of ProK (QIAGEN ...
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bioRxiv - Neuroscience 2022Quote: ... one 5 mm bead per sample was used in a TissueLyser (Qiagen) for 4 min at 30 Hz ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg/mL DNase I and 200 µg/mL RNase A (Qiagen). Followed by a mild sonication (10 strokes ...
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bioRxiv - Microbiology 2023Quote: ... maintained on ice and homogenized using 5 mm stainless steel beads (Qiagen) on a minibeadbeater (BioSpec ...
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bioRxiv - Microbiology 2023Quote: ... Harvested cells were fixed overnight in 5 mL RNA Protect reagent (Qiagen) after incubation with compounds ...
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bioRxiv - Microbiology 2023Quote: ... 5-10g of peat materials (preserved in Lifeguard soil preservation solution, Qiagen) was added into bead tubes ...
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bioRxiv - Biochemistry 2022Quote: ... and purified by affinity chromatography using a 5 mL Ni-NTA (Qiagen) column ...
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bioRxiv - Bioengineering 2023Quote: ... on a 5-plex QIAcuity One digital PCR instrument (911021, Qiagen, USA). The thermal cycling conditions were implemented using the following program ...
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bioRxiv - Cancer Biology 2023Quote: ... we manually dispensed 5 μL of Vapor-Lock (Qiagen, cat. no. 981611) into each well in the targeted region of a 384-well plate ...
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bioRxiv - Biophysics 2024Quote: ... corresponding to ∼5 L per sample (RNeasy PowerSoil Total RNA Kit; Qiagen). The RNA was tested with Bioanalyzer ...
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bioRxiv - Microbiology 2024Quote: ... 5 PCRs were pooled and purified on one Qiaquick spin column (Qiagen) following manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... 5 ml of the culture was mixed with RNAprotect Bacteria Reagent (QIAGEN) at a 1:2 ratio ...
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bioRxiv - Microbiology 2024Quote: ... (5) DNA was purified after reverse crosslinking using a MinElute column (Qiagen) as directed and quantified by a Qubit fluorometer (Invitrogen).
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bioRxiv - Evolutionary Biology 2024Quote: ... trigynum homostyle=5 individuals) using the RNeasy Plant Mini Kit (QIAGEN, Germany). Libraries were sequenced on an Illumina NovaSeq S1 Sequencing System to produce paired-end 150bp read length reads ...