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Citations for Qiagen :
501 - 550 of 1604 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Cells were grown in 6-well culture dishes to 70-80% confluency and RNA was isolated with RNeasy Plus kit (#74034, Qiagen). CDNA was generated using High Capacity cDNA Reverse Transcription Kit (#4368814 ...
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bioRxiv - Neuroscience 2024Quote: ... in vitro hTAM were incubated for 6 hours at 37°C with predesigned siRNA against human SPP1 (Qiagen, cat.#1027416) or Scramble (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... After 6 h RNA was extracted and1 µg of total RNA was used for reverse transcription (Quantitect reverse transcription kit, Qiagen) as per instructions ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from mouse prostate cell lines or WT prostate tissue from 8-10 week old C57BL/6 mice using the RNeasy Mini Kit (Qiagen). Complementary DNA (cDNA ...
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bioRxiv - Genetics 2024Quote: ... RNA was isolated from cultures incubated or not with 1 µM staurosporine for 6 hours to induce apoptosis using the RNeasy Mini Kit (QIAGEN). RNA samples were checked for quality on Agilent 4200 TapeStation System (Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysate was mixed with Ni-NTA agarose at 4℃ for 2h (Qiagen). Bound protein was eluted in 20 mM Tris pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... Lysates were treated with 4 μl of 100 mg/ml RNase A (QIAGEN) at 37 °C shaking at 500 RPM for 3 hours ...
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bioRxiv - Immunology 2021Quote: ... and kept at 4°C RNA was purified using RNEASY Mini Kit (Qiagen) and its concentration determined using a Nanodrop 1000 ...
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bioRxiv - Neuroscience 2021Quote: ... 4°C for 10 min and the pellet was lysed with RLT+ (Qiagen). For scRNA-sequencing ...
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bioRxiv - Microbiology 2021Quote: ... at 4°C until RNA was extracted using the RNeasy Mini Kit (Qiagen)
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bioRxiv - Microbiology 2020Quote: The MoBio PowerSoil-htp 96 kit (now Qiagen Cat No./Id: 12955-4), with minor modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... cell pellets were lysed in a 4% SDS buffer using a Qiashredder (Qiagen). Relative densitometry for western blots was determined using ImageJ software and normalized to the density of loading control β-ACTIN ...
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bioRxiv - Microbiology 2021Quote: ... into a volume of 4 μL PBS sc 1X (Qiagen, Cat. No. 150345). To avoid evaporation of the PBS sc 1X during the sort ...
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bioRxiv - Genomics 2021Quote: ... 4 μL of room temperature Stop solution (REPLI-g Single Cell Kit, Qiagen) was then added and the samples were vortexed and spun down ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with dsHsc70-4 with the Effectene Transfection reagent (301427, QIAGEN) and incubated for 72 h ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was incubated overnight at 4 °C with Strep-Tactin Beads (Qiagen), typically using 0.75 ml packed beads per two liters of original culture volume ...
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bioRxiv - Microbiology 2023Quote: ... supernatant was incubated overnight at 4°C with Ni-NTA agarose beads (Qiagen) used to purify the recombinant proteins as described [40] ...
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bioRxiv - Synthetic Biology 2022Quote: ... 18 µl of Omniscript enzyme at 4 U/µl (Omniscript RT Kit, Qiagen), and 37 µl water ...
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bioRxiv - Genomics 2023Quote: Approximately 3-4 million cells were solubilized with 1 mL QIAzol (Qiagen, 79306) and 0.2 mL chloroform in 5PRIME Phase-Lock Gel heavy tubes (QuantaBio ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Crystals were grown at 4 °C in EasyXtal-15-Well Tools plates (Qiagen), using hanging-drop vapour diffusion ...
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bioRxiv - Neuroscience 2023Quote: Cultured cells were lysed at 4°C in RLT buffer (Qiagen, Courtabœuf, France) and stored at -80°C ...
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bioRxiv - Microbiology 2024Quote: ... DNA was isolated with the DNeasy UltraClean 96 Microbial Kit (Qiagen 10196-4). Library preparation and sequencing were performed at the NGS Competence Center NCCT (Tübingen ...
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bioRxiv - Genomics 2024Quote: ... with 4 µl of RNase A (100 mg/ml) (Qiagen, Germantown, MD, USA), The extraction procedure was then followed by the manufacturer’s manual ...
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bioRxiv - Biochemistry 2024Quote: ... The soluble fraction was then added to 4 mL Ni-NTA beads (Qiagen) pre-washed in water ...
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bioRxiv - Genetics 2024Quote: ... Samples were cooled and 4 µL 100 mg/mL RNAse A (Qiagen, #19101) was added ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were harvested by scraping in 4 mL lysis buffer (RLT buffer (Qiagen) + 143 mM β-ME) ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from whole brain of 6 dpf larval zebrafish using RNeasy micro plus RNA extraction kit (Qiagen, Germany). Total RNA (2ng/µl ...
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bioRxiv - Genetics 2020Quote: ... and bands at 700 bp (insert) or 6 kb (plasmid library) cut and purified using QiaQuick Gel Extraction Kit (Qiagen #28706) with elution into 30 µL EB.
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bioRxiv - Evolutionary Biology 2021Quote: ... 2.5×106 cells were seeded per well in 6-well plates and transfected using Effectene transfection reagent (Qiagen, Cat. No. 301425). The transfection mixtures in Figure 5A contained 1 μg ...
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bioRxiv - Bioengineering 2021Quote: ... total RNA from the fibroblasts cultured with 10 μM Dwn1 was extracted every 6 hours from hours 24 to 48 (RNeasy® Plus Mini kit, Qiagen), followed by cDNA synthesis (SuperScript® VILO™ cDNA Synthesis Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA from untreated cells (24 h) and IFNγ-treated cells (6 or 24 h) were extracted with RNeasy plus mini kit (Qiagen, 74134) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: E3 and E4 primary microglia were plated at 5×106 cells/well in 6-well plates and RNA was extracted from the cells using the RNEasy Plus Mini Kit (Qiagen #74136) and converted to cDNA using High-Capacity RNA-to-cDNA kit (Thermo #4387406 ...
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bioRxiv - Microbiology 2021Quote: ... 40 μl of media from each of 6 plant wells or media from 3 minimal media wells were pooled and stabilized in RNAprotect® Bacteria Reagent (QIAGEN) before performing RNA extraction using RNeasy Mini Kit (QIAGEN) ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA from cells in a single well of a 6-well plate was isolated using the RNeasy Plus Mini Kit (Qiagen, 74134) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from cells (1-2 wells, 6 well plate) using an RNeasy Plus Mini Kit (Qiagen, Hilden, Germany), including a DNase step to remove residual DNA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was isolated from iPSC-SNs seeded in 6-well plates using a RNeasy Mini Kit (Qiagen, Redwood City, CA) and reverse transcribed into cDNA using a SuperScript VILO cDNA Synthesis kit (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown to 80% confluence in a 6-well plate before RNA was extracted using standard RNA extraction protocol (RNAeasy Mini Kit, Qiagen, #74104). cDNA synthesis was performed by mixing 1μg of RNA with qScript® cDNA Synthesis Kit (Quantabio ...
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bioRxiv - Genetics 2021Quote: ... S2 cells were seeded at 1 × 106 cells/ml in a 6-well plate and transfected using Effectene (Qiagen, Germantown, MD). For mobility shift assay (Figure 4) ...
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bioRxiv - Immunology 2023Quote: DNA was extracted from 1×10^6 CD4 Th cells/ replicate using the Qiagen DNA mini kit according to manufacturer’s instructions (Qiagen Hilden, Germany). DNA was extracted from 10ng of mouse brain tissue as previously described13 using NEB Monarch High molecular weight DNA extraction kit according to manufacturer’s instructions (NEB Cat #T3050).
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bioRxiv - Microbiology 2023Quote: DNA was extracted from GWSS and control samples (nGWSS = 79, ncontrol = 6) using a DNeasy PowerSoil DNA Isolation kit (Qiagen, Germany) with minor changes to the manufacturer’s protocol as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... BON1/TGL/pQ and BON1/TGL/HA-Bcl-xL) grown on 6-cm dishes were used to isolate mRNA using RNeasy Plus Mini kit (Qiagen, 74136) containing gDNA Eliminator spin columns ...
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bioRxiv - Bioengineering 2023Quote: ... DNA was extracted in pools of 6-8 adult mosquitoes mosquitoes using Blood & Cell Culture DNA Midi Kit (Qiagen, Cat# 13343) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: Total RNA from hippocampus samples (n=6 per group) was extracted using RNABle lysis reagent (Eurobio) and the RNeasy® Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... RNA and protein were extracted 6 days post-transduction using the AllPrep RNA/Protein kit (Cat: 80204, Qiagen, Germantown, MD USA). RNA was converted to cDNA using SuperScript IV cDNA Synthesis Kit (Cat ...
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bioRxiv - Immunology 2023Quote: ... in vitro derived Foxp3+ cells were harvested on day 2 and day 6 into Trizol and total RNA was isolated with miRNeasy Micro Kits (QIAGEN 217084) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... and non-inoculated G305-3M leaf segments collected along 10 different time points (0, 3, 6, 9, 12, 16, 24, 36, 48, 72 hpi) using the RNeasy Plant Mini Kit (Qiagen, Germany). The cDNA was synthesized from total RNA using a qScript™ cDNA Synthesis Kit (Quantabio ...
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bioRxiv - Physiology 2022Quote: ... liver and eWAT samples (n=6 per group) were extracted using RNABle lysis reagent (Eurobio) and the RNeasy® Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was isolated from cell pellets in batches of 6 using an RNeasy Plus Mini Kit (Cat # 74134, Qiagen, Hilden, Germany). Acceptable RNA concentration and quality were verified with Nanodrop and Bioanalyzer measurements ...
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bioRxiv - Microbiology 2024Quote: Microbial DNA was extracted from fecal samples of C57BL/6 mice using the QIAamp DNA Fecal Microkit (Stras Qiagen GMBH, Germany). A NanoDrop 2000 spectrophotometer (Thermoelectric Science ...
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bioRxiv - Cell Biology 2024Quote: APOB expression determined by first isolating total RNA from Hep3B cells grown in 6-well plates using the RNeasy Mini Kit (Qiagen, #74104). Real-time qPCR was performed using the TaqManTM RNA-to-CtTM 1-Step Kit (ThermoFisher ...