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Citations for Qiagen :
501 - 550 of 1199 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K4ac/K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Microbiology 2022Quote: ... 100ng of RNA was converted to cDNA for 1h at 37 °C using the Omniscript RT kit (Qiagen) and random primers (Promega) ...
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bioRxiv - Biophysics 2022Quote: ... The suspension was spun down at 16,000 r.c.f at 4 °C for 30 min and the supernatant was applied 5 ml Ni-NTA resin (Qiagen)/ L culture medium ...
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bioRxiv - Immunology 2021Quote: ... Granuloma homogenate was taken for RNA isolation using RNeasy Plus Kits and stored at −80°C (Qiagen, USA). Quantification of viral burden was done using TaqPath 1-step RT-qPCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... the pellet was stored at -80°C before RNA extraction using the Qiagen RNeasy Mini kit (Qiagen, USA), following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... for 30 min at 37°C and cleaned up with the RNeasy MiniElute cleanup kit (Qiagen, Hilden, Germany) following the manufacturers’ instructions ...
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bioRxiv - Pathology 2020Quote: ... diffracting crystals of P1_102-157 were obtained at 4°C from condition 7 of the Classic kit (Qiagen) containing 0.1 M tri-Sodium citrate pH 5,6 ...
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bioRxiv - Microbiology 2021Quote: ... for 30 minutes at 37°C followed by beadbeating with 50μg 0.1mm zirconium beads for 6 minutes on the Tissuelyzer II (Qiagen) prior to loading onto the Qiacube HT ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 30 min at 45,000 × g at 4°C and incubated with 0.6 ml of Ni-NTA agarose beads (Qiagen) per 1 l of expression culture (1 ml beads per 1 l expression culture was used in case of Drosophila BiP ...
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bioRxiv - Neuroscience 2021Quote: ... 37 °C) with 50 μg RNA sample and purified using RNeasy Protect Mini Kit (catalog no. 74124, Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... IL2-Rα ectodomain was produced with C-terminal 6xHis tag and purified on Nickel-NTA spin columns (Qiagen) according to manufacturer instructions.
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bioRxiv - Cancer Biology 2022Quote: ... for 24 hours at -20°C prior to DNA/RNA extraction with AllPrep DNA/RNA Mini Kit (Qiagen). Cryopreserved patient ascites cells and PDX cancer spheroids were thawed and rinsed in serum free RPMI before RNA/DNA extraction ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA was eluted from the column using 5 mL of prewarmed (50°C) QF Buffer (Qiagen, Germany). DNA was precipitated by adding 0.7 volumes of isopropanol ...
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bioRxiv - Neuroscience 2022Quote: ... Reaction was incubated at 37 °C for 30 min and purified using the QIAGEN MinElute Kit (28004 QIAGEN) according to the manufacturers protocol ...
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bioRxiv - Immunology 2022Quote: ... Tregs were pelleted and stored at -80°C in RTL lysis buffer (RNeasy micro kit, Qiagen, Hilden, Germany) with β-mercapto-ethanol (1:100) ...
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bioRxiv - Genetics 2022Quote: Liver samples were stored frozen at −80 °C before RNA extraction with the RNeasy kit (QIAgen, Crawley, UK) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Inhibitors (antimiRs) against miR26b-5p and miR200a/b/c-3p were purchased from Qiagen (339130 and 339160 respectively). AntimiRs in injection media (1mM Tris-HCL pH 7.5 and 0.5 mM EDTA in embryo grade water ...
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bioRxiv - Immunology 2024Quote: ... 2 × 104 cells were sorted in triplicate per sample and stored at −80°C in RLT buffer (Qiagen). RNA was isolated using the Qiagen RNeasy Micro Kit ...
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bioRxiv - Microbiology 2023Quote: ... homogenized samples were stored at −20 °C prior to extraction using the DNeasy® PowerSoil® Kit (Qiagen) following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... at -20°C or immediately processed for RNA using the RNeasy Mini Kit as per manufacturer’s protocol (Qiagen). Real-time analyses were performed as previously described (Van Otterloo et al. ...
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bioRxiv - Biochemistry 2022Quote: ... 4 °C for 1 h and purified by affinity chromatography using a 5 ml Ni-HP column (Qiagen). Flow through with pure AstaPo1 was collected and dialyzed overnight against the buffer containing 50 mM Tris pH 7.6 ...
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bioRxiv - Genomics 2023Quote: ... or the buffy coat of the BALB/c strain using the QIAsymphony DNA Mini Kit (cat:931236, Qiagen). The extracted gDNA was sheared to 150 bp in size using a Covaris LE 220 instrument ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% CA630) and rotated at 4°C for 2 hours before addition of His-beads (Ni-NTA, QIAGEN). After continuous rotating for another 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... 10 minutes 4°C and the supernatant was incubated with pre-equilibrated Ni-NTA agarose beads (Qiagen, #1018244) with shaking on ice for 3 hours ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA was stored at −80°C until further clean-up using RNeasy Mini kit (Qiagen, catalog #74104) manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... One hour of tagmentation at 37°C was followed by DNA extraction using MinElute PCR Purification Kit (Qiagen). Extracted DNA was subjected to PCR amplification using unique primers sets (Nextera XT v2 Full set (N7-S5)) ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was incubated at 37 °C for 15 min and then quenched by adding RLT buffer (Qiagen). The RNA was then extracted using RNeasy kit (Qiagen) ...
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bioRxiv - Immunology 2024Quote: ... Genomic DNA from the Swedish cohort was extracted from whole blood using Puregene Blood Core Kit C (Qiagen). Illumina whole-genome sequencing ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria were centrifuged at 4°C and washed with pre-cold PBS containing RNA stabilization solution (RNAprotect, Qiagen) before flash freezing of the pellets and storage at -80°C ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from –80 °C-stored microcosm recovered cell samples using the DNeasy PowerSoil Pro kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... the homogenates were incubated overnight at 56°C with 20 µL of 20 mg/mL protease K (Qiagen). The crude extract was purified using the QIAamp DNA Mini Kit (Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... The samples were then placed at −80°C until grinding by a tissue-lyser (TissueLyser II, Qiagen, Germany).
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bioRxiv - Biophysics 2024Quote: ... for 4 hours at 37°C and purified from a 2% agarose gel (QIAquick Gel Extraction Kit, Qiagen). At this stage ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was the incubated at 4 °C with 2 ml of washed Ni-NTA agarose beads (Qiagen) for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were incubated on a Thermomixer (37°C, 30 min, 500 rpm) before being subjected to MinElute (Qiagen) cleanup to extract tagmented DNA ...
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bioRxiv - Developmental Biology 2020Quote: ... we differentiated human stem cells for 3 days in mTeSR + 0.5 µM A8301 and extracted RNA with RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic DNA from 3×107 sorted GFP+ cells was extracted using the Blood & Cell Culture DNA Maxi Kit (Qiagen). Amplification of sgRNA regions from the extracted genome and the original sgRNA plasmid library ...
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bioRxiv - Molecular Biology 2020Quote: ... while method-3 was a modified protocol of the DNeasy PowerMax Soil Kit (Cat.No. 12988-10) provided by Qiagen (based on communication exchanged with the manufacturer) ...
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bioRxiv - Genomics 2021Quote: ... Total RNA was purified from cell passage number 3 using the RNeasy RNA purification kit (Qiagen Cat. No. 75142). A260:280 ratio > 2 and RIN > 9.
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bioRxiv - Immunology 2021Quote: Bacterial plasmid DNA was extracted from a 3 ml overnight culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104). Next ...
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bioRxiv - Microbiology 2020Quote: Each dissected compartment (legs and thoraxes) was homogenized individually 3 x 1 minute at 30 Hertz using TissueLyser (Qiagen) and glass beads (#11079110 ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue samples were homogenized in 3 mL sterile PBS for 20 seconds on ice using a TissueRuptor homogenizer (Qiagen) with sterile tips ...
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bioRxiv - Genomics 2022Quote: ... 50 ng of total RNA was processed up to 3’ adapter ligation step according to the manufacturer’s instruction (Qiagen). The adapter-ligated RNA was treated with 5U of RppH (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted at 3 hpi and 24 hpi with the RNeasy Mini Total RNA extraction kit (Qiagen). SARS-CoV-2 RNA was detected with the CDC assay kit (IDT ...
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bioRxiv - Bioengineering 2022Quote: ... transfected cells were harvested on Day 3 and genomic DNA was extracted using DNeasy Blood & Tissue Kit (QIAGEN # 69506). The region surrounding EMX1 target site was amplified with EMX1-F ...
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bioRxiv - Neuroscience 2021Quote: ... miR-67 (miR control) or empty vector at a ratio of 1:3 using a lipofection protocol (Effecten, Qiagen) and processed at DIV15 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were mechanically disrupted by bead-beating for 2 × 3 min at 30 Hz (TissueLyser II, Qiagen, Hilden, Germany). The samples were incubated at −80°C for 10 min and at 95°C for 10 min ...
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from 3 dpf and 6 dpf cdipt mutant zebrafish and their wildtype siblings using RNAeasy (Qiagen). RNA samples were reverse transcribed into cDNA using the iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Developmental Biology 2020Quote: ... using the QIAGEN TissueLyser at 15 Hz for 2-3 min with a Stainless-Steel Bead (QIAGEN catalog # 69989). Phase separation was induced with chloroform ...
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bioRxiv - Genetics 2020Quote: ... Seedlings of 3-week-old plants were used to extract total RNAs using the RNeasy Plant Mini Kit (Qiagen). Total RNAs were treated with amplification-grade RNase-free DNase I (Qiagen ...