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Citations for Qiagen :
5251 - 5300 of 10000+ citations for C Reactive Protein CRP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Skin and intestinal samples were handled aseptically and skin-associated DNA was extracted using a NucleoSpin Soil Extraction Kit (Machery-Nagel GmbH, Düren, Germany) while the intestine-associated DNA was extracted using MoBio DNA Extraction Kit (QIAGEN, Hilden, Germany) both as described (Hamilton et al. ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from the cortical brain and cochlea samples from 1-month-old mice using RNA extraction kit and Qiazol Reagent (RNeasy mini kit; Qiagen, Germany), and DNase treatment was performed (RNase-free ...
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bioRxiv - Molecular Biology 2023Quote: ... and the RNA-containing aqueous phase was subjected to a column purification kit using RNeasy Mini RNA extraction kit (Qiagen, USA). RNA was DNase treated ...
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bioRxiv - Genetics 2023Quote: Clones obtained from three independent conjugation assays were collected and subjected to genomic extraction using the kit DNeasy® Tissue Kit (Qiagen). DNA was mechanically fragmented using the Covaris method (DNA shearing with sonication) ...
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bioRxiv - Immunology 2023Quote: The DNA/RNA samples were isolated using either the AllPrep DNA/RNA Mini Kit or the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: QIAamp DNA Blood Mini Kit (for whole blood, buffy coat and plasma) and QIAamp DNA Mini Kit for lesion scraping (Qiagen, Germany) were used for DNA extraction as per the manufacturer’s protocols except elution in 50μl elution buffer AE (10 mM Tris-Cl ...
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bioRxiv - Developmental Biology 2023Quote: ... From tissues and clotted blood samples DNA was extracted manually using QiaAmp DNA Blood Midi Kit and the DNeasy Tissue Kit according to manufacturer’s protocol (Qiagen, Valencia, CA). DNA from BA10 was extracted on an automated nucleic acid extraction platform Anaprep (Biochain ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Then the DNeasy PowerPlant Pro Kit protocol was followed and the extracted DNA was further purified with DNeasy PowerClean CleanUp Kit (Qiagen, Germany).
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was extracted from the thoracic muscle tissue of each individual using either the QIAamp 96 DNA Blood Kit or the DNeasy Blood & Tissue Kits (Qiagen, LLC). Libraries were prepared for low-coverage whole genome sequencing (2×150 bp reads ...
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bioRxiv - Microbiology 2023Quote: ... gene in lung tissues were determined using 2019-nCoV RUO Kit (Integrated DNA Technologies #10006713 and QuantiNova SYBR Green PCR kit (Qiagen #208052) according to the following cycling program ...
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bioRxiv - Cancer Biology 2024Quote: The DNA/RNA samples were isolated using either the AllPrep DNA/RNA Mini Kit or the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... The DNA was extracted directly from the frozen samples using a commercial kit (DNeasy Power Soil Pro Kit, QIAGEN, Hilden, Germany), following the instructions of the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was extracted for total RNA sequencing using the Direct-zol RNA Miniprep Kit (ZymoResearch R2050) or the miRneasy Mini Kit (Qiagen 217004) according to manufacturer protocols ...
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bioRxiv - Immunology 2024Quote: ... Astrocytes were isolated using the Anti-ACSA-2 MicroBead Kit (Miltenyi, #130-097-678) and RNA extracted using RNeasy Micro Kit (Qiagen, # 74004). RNA library preparation ...
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA was extracted using either a QIAGEN HiSpeed® Plasmid Midi Kit or Maxi Kit (QIAGEN Sciences, Germantown, MD, USA) as per the manufacturer’s instructions (HiSpeed® Plasmid Purification Handbook) ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was purified with agarose beads that bind specifically to the His-tag (Ni-NTA Agarose, Qiagen) following the purification hybrid method from the ProBond purification system (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... mRNA expression levels were normalized to the housekeeping human TATA-binding protein (TBP) mRNA (RT2 qPCR Primer Assays, Qiagen) in the GAL4 assay and to the human housekeeping hypoxanthine guanine phosphoribosyltransferase (HPRT ...
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bioRxiv - Biophysics 2021Quote: ... The target protein was eluted by 0.2 mg/ml FLAG peptide and load to Ni-NTA affinity gel (Qiagen). After washed by Lysis Buffer supplied with 0.02% GDN and 0.004% CHS ...
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bioRxiv - Biochemistry 2020Quote: ... and the recombinant protein was purified by gravity-flow chromatography using a nickel-charged resin Ni-NTA Agarose (QIAgen) equilibrated with 10 mM imidazole in the lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: The genes encoding protein groups significantly altered in epilepsy and control were analyzed using Ingenuity Pathway Analysis (IPA, Qiagen). We analyzed all differentially expressed proteins in the hippocampus (n=777) ...
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bioRxiv - Microbiology 2021Quote: The pulldown protocol was based on the principle of immobilization of recombinant protein using Ni-NTA agarose resin (Qiagen). The experiments were performed in biological triplicates and designed to contain 4 control samples (resin Ni-NTA with i ...
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bioRxiv - Microbiology 2021Quote: ... and H2A.Z (fused with a maltose-binding protein -MBP) purification were performed by affinity chromatography using Ni-NTA agarose (Qiagen) and amylose resin (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was clarified by centrifugation at 13,000 rpm and protein was purified using Ni- NTA superflow resin (Qiagen), according to the manufacturer’s recommendations for a centrifugation-based protocol ...
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bioRxiv - Microbiology 2022Quote: ... Headless HA stalk proteins were expressed in 293F cells and purified using nickel-nitrilotriacetic acid agarose (no. 1018244, Qiagen) in 5-ml polypropylene columns (no ...
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bioRxiv - Biochemistry 2021Quote: ... was transformed with an N-terminally His-tagged fusion protein of cytHPPK/DHPS (At1g69190) in the vector pQE30 (Qiagen). Overnight cultures grown at 30°C in LB broth supplemented with 35 µg/mL kanamycin and 100 µg/mL ampicillin were sub-cultured to an OD600 of 0.6 - 0.7 and induced overnight at 16°C with 100 mM isopropyl β-D-1-thiogalactopyranoside ...
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bioRxiv - Biochemistry 2021Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Microbiology 2022Quote: ... N-terminal His-tagged SlPR1 and SlChi3 proteins were purified using Ni-nitrilotriacetic acid (Ni-NTA) affinity resin (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: The solubilized protein was incubated for 1 hour with washed and pre-equilibrated Strep-Tactin Superflow plus resin (Qiagen). The resin was then loaded into an Econo-Column Chromatography Column (Biorad ...
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bioRxiv - Microbiology 2020Quote: ... and 8 M urea [pH 8.0]) and His-tagged Tax proteins were precipitated with Ni-nitrilotriacetic acid (NTA) agarose (Qiagen). After washing in buffer C (100 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2020Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Synthetic Biology 2021Quote: ... After spinning down the insolubilized protein fraction the supernatant was loaded onto an equilibrated mini NiNTA spin column (Qiagen) and the flow-through was reloaded twice to maximize protein binding ...
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bioRxiv - Genomics 2021Quote: ... DNA gets separated from proteins and cellular debris using proteinase K (20mg mL-1; Qiagen, Inc., Valencia, CA, USA), precipitated with ethanol and cleaned up ...
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bioRxiv - Genomics 2022Quote: ... DNA and RNA was extracted from fresh-frozen tissue samples using the AllPrep DNA/RNA/Protein minikit (Qiagen, USA) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Recombinant SlERF.D7-6XHIS protein was purified by Ni2+gravity flow chromatography according to the manufactureer’s protocol (Ni-NTA Agarose, Qiagen) as described ...
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bioRxiv - Plant Biology 2022Quote: ... Recombinant RIN-6XHIS protein was purified by Ni2+ gravity flow chromatography according to the manufacturer’s protocol (Ni-NTA Agarose, Qiagen). For the EMSA assay ...
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bioRxiv - Biochemistry 2022Quote: ... Culture medium was harvested 72 h post-transfection and secreted His-tagged proteins purified using Ni-NTA agarose (Qiagen) in batch mode ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant proteins were purified to near homogeneity (>95%) using Ni-chelate affinity chromatography on Ni-NTA Superflow resin (Qiagen) using standard protocols ...
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bioRxiv - Genetics 2023Quote: ... Precipitated proteins were resuspended in 6M guanidine hydrochloride and incubated with 25 µL of Ni-NTA agarose beads (Qiagen) overnight ...
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bioRxiv - Immunology 2023Quote: ... The supernatant containing target proteins was passed through a polypropylene column containing 2 ml Ni-NTA resin from Qiagen. After the target protein bound to the Ni-NTA resin ...
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bioRxiv - Microbiology 2023Quote: ... the soluble fraction containing the His-VitR protein was purified using NTA-resin affinity chromatography in phosphate buffer according to manufacturer’s recommendations (Qiagen). After concentration (Vivaspin 6 Concentrator ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant protein was purified by affinity chromatography using nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (Qiagen, http://www.qiagen.com) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant protein was purified by affinity chromatography using nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (Qiagen, http://www.qiagen.com) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: The organs of Corti of postnatal day (P)4 mice were dissected and stored at -20°C in RNAprotect Tissue Reagent (RNAlater®) (QIAGEN, cat. no. 76106). Total RNA was extracted using the SPLIT RNA extraction kit (Lexogen ...
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bioRxiv - Genomics 2021Quote: ... the detection of N-gene of SARS-CoV-2 was performed by using the 2019-nCoV-2 RUO kit (Integrated DNA Technologies, Inc., Coralville, Iowa, USA) and One-Step RT-PCR Kit (QIAGEN® GmbH) on a Rotor-Gene Q real-time PCR cycler (QIAGEN® GmbH) ...
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bioRxiv - Immunology 2021Quote: ... Total RNA from the cells and EVs were first isolated and purified using an RNeasy Mini Kit and a miRNeasy Serum/Plasma kit (Qiagen, Hilden, Germany), respectively ...
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bioRxiv - Genomics 2019Quote: Joint RNA/DNA extractions were performed with the RNA PowerSoil Total RNA Isolation Kit combined with the RNeasy PowerSoil DNA elution kit (MO BIO Laboratories, Inc.; Qiagen, Hilden, Germany). About 5 g of sediment were used ...
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bioRxiv - Pathology 2019Quote: Taxonomy filtered from samples was determined by analysis of kit controls with no template and zymo sequencing controls of know diversity and abundance (the QIAamp DNA Microbiome Kit (Qiagen, Hilden, Germany) DNA Clean and Concentrator Kit (Zymo Research) ...
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bioRxiv - Neuroscience 2019Quote: ... and total RNA was isolated by using the RNeasy Lipid Tissue Mini Kit or RNeasy Plus Universal Mini Kit (Qiagen, Hilgen, Germany). Complementary DNA was synthesized by using the Omniscript RT Kit (Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA and genomic DNA were extracted using, respectively, the InviTrap® Spin Universal RNA Mini Kit (Stratec Biomedical, Germany) and the DNeasy Blood & Tissue Kits (Qiagen, Germany) according to manufacturers’ instruction ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA was extracted from clarified cell culture supernatants (16,000 g x 10 min) and from infected cells using QIAamp Viral RNA Mini Kit and RNeasy Plus mini kit (Qiagen, Hilden, Germany), respectively ...