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Citations for Qiagen :
5151 - 5200 of 10000+ citations for C Reactive Protein CRP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and miRNeasy mini kit were purchased from Qiagen, Netherlands ...
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bioRxiv - Immunology 2024Quote: Total RNA was obtained using RNeasy kits (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted with RNeasy mini Kit (Qiagen) following manufacturer recommendations ...
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bioRxiv - Microbiology 2024Quote: The DNeasy PowerSoil Pro Kit (Qiagen Cat 47016) was used as per manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... and extracted using QIAquick gel extraction kit (Qiagen). The final libraries were subjected to QC (size ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was purified (Qiagen MinElute PCR purification kit) and PCR amplified (7 cycles ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and purified using a RNeasy Mini Kit (Qiagen). Single-stranded cDNA was synthesized from 2 µg of total RNA using a High-Capacity cDNA Archive Kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated with a RNeasy kit (Qiagen) prior to a quality control check using an Agilent Bioanalyzer and NanoDrop One spectrophotometer ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted using an Allprep kit (Qiagen), following the manufacturer ’s protocol ...
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bioRxiv - Genetics 2024Quote: ... and a DNeasy Blood and Tissue Kit (Qiagen). Frozen tissue samples were homogenised by adding 600 uL RLT containing 1% β-Mercaptoethanol and a 5mm stainless steel bead (Qiagen # 69989 ...
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bioRxiv - Immunology 2024Quote: ... RNA was extracted using the RNeasy Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and DNeasy PowerSoil Kit (Catalog# 12888-100, Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Isolation was performed using RNAesy Mini Kit (Qiagen) according to the protocol ...
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bioRxiv - Genomics 2024Quote: ... using the RNeasy RNA Isolation kit (Qiagen# 74104) as per instructions provided by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... This was incubated in a 37 °C shaker for 1 hour before the addition of Proteinase K (10 µl, QIAGEN, >600 mAU / ml) and left at 55 °C for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: Data to derive different biological clocks was available for different subsamples and all based on a fasting blood draw from participants in the morning between 8:30 and 9:30 after which samples were stored in a −80°C freezer or – for RNA - transferred into PAXgene tubes (Qiagen, Valencia, California, USA) and stored at −20°C ...
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bioRxiv - Cell Biology 2022Quote: ... The reaction mixture was incubated at 37°C for 24 hours and subsequently underwent RNA clean-up using a column-based purification (Qiagen, product number 74104). The purified RNA was eluted in nuclease-free water (Qiagen ...
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Macrobdella decora: Old World Leech Gut Microbial Community Structure Conserved in a New World LeechbioRxiv - Microbiology 2019Quote: ... The supernatant was transferred to clean 1.5mL MCF tube and to which 350 μL ethanol at 4’C was added before vortexing for 10 sec and transferring the supernatant to a QIAamp Mini spin column (Qiagen Germantown, MD U.S.A.). The sample was process as recommended in the QIAamp DNA Mini Handbook and eluted with 10 mM Tris-Cl ...
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bioRxiv - Microbiology 2022Quote: ... The soluble fraction was separated from debris by centrifugation (9000 g, 4°C) and incubated for 60 minutes with Ni-NTA beads (Qiagen, cat. no. 30230) pre-equilibrated in binding buffer ...
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bioRxiv - Biophysics 2024Quote: ... for 30 min at 4 °C before being applied to a 10mL bed volume Nickel nitrilotriacetic acid (Ni-NTA) agarose (Qiagen, cat. no. 30230) column ...
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bioRxiv - Neuroscience 2021Quote: ... A 5 mm stainless steel bead was subsequently used to homogenize the tissue using the TissueLyser II system (Qiagen) for 2x 5 min at 20 hz ...
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bioRxiv - Genomics 2020Quote: ... then PCR-amplified using the PyroMark PCR kit using Qiagen’s pyrosequencing primer assays or those designed in-house (Suppl. Table 5) via the PyroMark Assay Design Software 2.0 (Qiagen). Reaction conditions were as follows ...
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bioRxiv - Microbiology 2021Quote: ... was performed by using 2.5 μL of the synthesized and diluted cDNA in the presence of 5 μL QuantiTect SYBR Green (204143, Qiagen) and 1 mM specific primers (S6 Table ...
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bioRxiv - Synthetic Biology 2021Quote: ... the biofilms were disrupted by pipetting thoroughly and homogenized with a stainless bead beater 5 mm in diameter (Qiagen) for 10 min at a frequency of 30 beats per second by TissueLyser II (Qiagen) ...
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bioRxiv - Physiology 2021Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 g for 10 minutes at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... protein yields of the CNA35 probe were synthesized using E.Coli bacteria before purification using ÄKTApurifier (Cytiva) and a 5 ml Ni-NTA Superflow Cartridge (Qiagen), dialysis with SnakeSkinTM Dialysis tubing with 10 kDa MWCO ...
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bioRxiv - Cancer Biology 2022Quote: ... each well was incubated in 75 μL 0.08 M KOH for 5 min and washed with 100 μL EB buffer (QIAGEN) at room temperature (RT) ...
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bioRxiv - Microbiology 2022Quote: ... bacterial pellets were resuspended and incubated at room temperature for 5 mins in 500µl RNAprotect® bacteria reagent (QIAGEN). Samples were centrifuged at 16000g for 5 mins and supernatant removed ...
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bioRxiv - Microbiology 2022Quote: ... and microbial Eukaryotic abundance with a QIAcuity One 5-plex digital PCR (dPCR) instrument (Qiagen Inc. USA, Germantown, MD). dPCR samples represented both the 416 Fire —across SBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue samples (5-25 mg) were kept on ice and submerged in RNAlater RNA stabilization reagent (QIAGEN, Hilden, Germany) within 30 minutes of collection and stored at −80°C.
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bioRxiv - Microbiology 2020Quote: ... 5 μl of cDNA was then added to a PCR mix containing 12.5 μl 2× Multiplex PCR mix (Qiagen), 9 μl H2O ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... of vehicle and Acyl-GIP vehicle treated LDLR−/− mice (n = 5) using Qiazol according to the manufacturer’s instructions (Qiazol Lysis Reagent, QIAGEN). The quality of the RNA was determined with the Agilent 2100 BioAnalyzer (RNA 6000 Nano Kit ...
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The mitochondrial genome of the red icefish (Channichthys rugosus) casts doubt on its species statusbioRxiv - Zoology 2022Quote: ... the lysates were centrifuged at 17,000 x g for 5 minutes and 300 μL supernatant mixed with 3000 μL Buffer PB (Qiagen). The mixtures were loaded onto Minelute silica columns (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... accordingly to manufacturer’s instructions using non-targeting siRNA (siCtrl; 5’-AATTCTCCGAACGTGTCACGT-3’) and siRNA targeting Cav1 (SI00299635 and SI00299628) from Qiagen, or using pre-miR-NC (negative control ...
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bioRxiv - Developmental Biology 2019Quote: ... The TSB (5’-ATTATTGCACCCAGTGCC-3’: the miR-92a-3p target site is underlined) was designed and produced by Qiagen, with an additional scrambled TSB to act as a negative control (5’-TAACACGTGTATACGCCCA-3’) ...
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bioRxiv - Physiology 2020Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 rcf for 10 minutes at 4°C ...
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bioRxiv - Physiology 2021Quote: ... the supernatant was harvested by centrifugation for 20 min at 10,000 g and then loaded onto 5 ml Immobilized Metal-ion Affinity chromatography (IMAC, Ni-NTA Agarose, Qiagen) pre-equilibrated in 20 mM Hepes pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... Epithelial tissue and chorion were individually sampled from 3 to 5 consecutive sections and each microdissected tissue was immediately placed in 80μL of ice-chilled RLT buffer (Qiagen) and stored at −20°C until RNA extraction ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were homogenized at 25–30 Hz for 5 min using a tissue homogenizer (QIAGEN TissueLyzer II, Hilden, Germany). Samples were swabbed with sterile dual tip swabs (BD swubes ...
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bioRxiv - Physiology 2021Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 rcf for 10 minutes at 4C ...
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bioRxiv - Physiology 2021Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 rcf for 10 minutes at 4°C ...
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bioRxiv - Biochemistry 2021Quote: The LRH-1-10CA-TIF2 complex was concentrated to 5 mg/mL and screened using the Classics screen (Qiagen) and a Phoenix Liquid Handler (Art Robbins Instruments ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with siRNA as above (Lap2: 5’-GAUGUGACAGAGCUCUCUA from Sigma; negative control: AllStars negative control from Qiagen). Total RNA was extracted with a Nucleospin RNA kit from Macherey-Nagel according to the manufacturer’s protocol from triplicates of control and Lap2-depleted samples ...
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bioRxiv - Immunology 2020Quote: ... which contained 5 μl of sort buffer consisting of (1X Qiagen One-step RT PCR Buffer (Qiagen, Hilden, Germany), 0.1 mM dithiothreitol (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Enriched cells were surface stained and 5-20 ×103 intratumoral cDC1s were sorted directly in RLT lysis buffer (Qiagen) containing 2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2022Quote: ... Transcribed cDNA was diluted 5-fold and used for real-time quantitative PCR (QuantiTect SYBR Green; Qiagen ref: 204145). RNA-sequencing libraries were produced with the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (E7770) ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was loaded onto a 10 mL Ni-NTA Superflow cartridge (connected two 5 mL cartridges) (30761, Qiagen). The cartridge was washed with 100 mL R buffer containing 200 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble fraction was recovered by centrifugation at 40 000 × g in a JLA25.50 rotor and put through a gravity flow column with 5 ml of NiNTA Agarose (Qiagen). Bound fraction was washed in Buffer B (300 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... Total cellular DNA was extracted from approximately 0.5 to 5 million cells using a QIAamp DNA Blood Minikit (Qiagen). PCR assays were performed with 200 ng samples of DNA ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were filtered through a 0.22 µm syringe filter before application to 5 ml of nickel resin (Ni-NTA Superflow, QIAGEN) equilibrated in loading buffer (25 mM HEPES-KOH pH 7.6 ...