Labshake search
Citations for Qiagen :
451 - 500 of 2287 citations for PEGSSDA 2 x 0.5 mL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
INTEGRATE: Model-based multi-omics data integration to characterize multi-level metabolic regulationbioRxiv - Systems Biology 2021Quote: Total RNA was extracted from at least 8 x 106 cells by using RNeasy Mini Kit (Qiagen). Each pellet was resuspended by adding 30 μL of RNAse-free water ...
-
bioRxiv - Biochemistry 2020Quote: Total RNA was isolated from 1.0 x 106 HEK293T cells by using the RNeasy Mini Kit (Qiagen) in combination with the QIAshredder system (Qiagen ...
-
bioRxiv - Microbiology 2021Quote: ... The extraction was performed in a QIAGEN-Corbett X-tractor Gene automated DNA/RNA extractor (QIAGEN, Australia) using the default settings for RNA extraction ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were harvested by centrifugation for 10 min at 3200 x g and resuspended in RNAprotect (Qiagen). RNA was isolated using the RNeasy mini kit (Qiagen) ...
-
bioRxiv - Bioengineering 2019Quote: RNA was extracted from ~1.9 x 106 transfected HEK293T cells using the RNeasy Mini Kit from Qiagen with the QIAshredder for homogenization ...
-
bioRxiv - Immunology 2020Quote: ... Cells were then washed 3 x with ice-cold 1x PBS and lysed in RLT buffer (Qiagen) with 1/100 β-mercaptoethanol (Sigma ...
-
bioRxiv - Molecular Biology 2021Quote: ... which were excised by Gel X-tracta tool (USA scientific) and purified with Gel Extraction Kit (Qiagen). The purified DNA material was PCR-amplified (18 cycles ...
-
bioRxiv - Molecular Biology 2020Quote: The small RNAs from the yeast cells (1 x 107) were isolated with the miRNeasy kit (Qiagen) with modifications ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cleared lysate was applied to a 20 ml disposable gravity-flow column 1.5 ml (0.75 ml bed volume) of NI-NTA agarose (Qiagen cat. #30210), washed twice in three bed volumes of Lysis Buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 µg/mL DNase I and 200 µg/mL RNase A (Qiagen). Followed by a mild sonication (10 strokes ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.7 ml Qiazol (Qiagen) was added to each dish and the lysate was collected into Qiashredder tubes (Qiagen ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 1x Coral Load, 400 nM of each primer, 160 nM dNTPs and 0.5 unit of Top Taq Polymerase, Qiagen). Subsequently we used 5 μL out of 50 μL macerate extract to amplify the mitochondrial cytochrome C oxidase I (COI ...
-
bioRxiv - Developmental Biology 2020Quote: ... we differentiated human stem cells for 3 days in mTeSR + 0.5 µM A8301 and extracted RNA with RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNA was extracted from cells maintained in low-serum conditions (0.5% calf serum) for 22 hours using RNeasy purification kit (Qiagen) and treated with DNase on column ...
-
bioRxiv - Zoology 2020Quote: ... Two independent replicates were performed in 8 μl of volume containing 0.5 U of Hot Start Taq polymerase (Qiagen), 0.18 μM of each primer and 0.04 mg of Bovine Serum Albumin Fraction V (Roche) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Pyrosequencing of the PCR products was performed using 0.5 µM of sequencing primer on the PSQ96 HS System (Pyrosequencing, Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2019Quote: Frozen tissues (0.5-1cm3) from the following brain regions from each case were macrodissected into RNAlater RNA Stabilization Reagent (Qiagen): 1 ...
-
bioRxiv - Genomics 2019Quote: ... Discs were dissected on ice in BPS with 0.5% BSA and transferred to 350 μl of RLT buffer (Qiagen) with 1% β-mercaptoethanol (BME) ...
-
bioRxiv - Plant Biology 2020Quote: ... and the basal part of inflorescence stems (about 0.5 cm above rosette base) using the RNEasy© kit (Qiagen). First-strand cDNA was synthesized using the RevertAid RT Reverse Transcription kit (Thermo Fischer Scientific) ...
-
bioRxiv - Plant Biology 2020Quote: ... and the basal part of inflorescence stems (about 0.5 cm above rosette base) using the RNEasy© kit (Qiagen). First-strand cDNA was synthesized using the RevertAid RT Reverse Transcription kit (Thermo Fischer Scientific) ...
-
bioRxiv - Genetics 2022Quote: ... 10 ng of each sample (technical triplicates of 3 independent biological replicates) was amplified using various primers (0.5 µM; Table S2) and SYBR Green PCR master mix (QIAGEN). Reactions were run for 44 cycles on a StepOnePlus Real-Time PCR machine (Applied Biosystems ...
-
bioRxiv - Microbiology 2022Quote: Frozen tissue was partially thawed and submerged in lysis buffer containing 1% β-mercaptoethanol and 0.5% Reagent DX (Qiagen) before tissues were homogenised together with TissueRupture (Qiagen) ...
-
bioRxiv - Genomics 2019Quote: ... the DNA was further purified by mixing the sample with a 0.5 volume of CU and a 0.5 volume of IR solution from the Qiagen DNeasy PowerClean Pro Clean Up Kit (Qiagen), centrifugation for 2 min at 15,000 rpm at room temperature and DNA precipitation from the supernatant using a 2.5 volume of 96% ethanol ...
-
bioRxiv - Genetics 2020Quote: ... AC7594-5’-CCGCCTATCCTCGTCATGAAC)andcontrolALG9primers(AC5067-5’-CACGGATAGTGGCTTTGGTGAACAATTAC;AC5068-5’-TATGATTATCTGGCAGCAGGAAA GAACTTGGG) (0.5 µM) and QuantiTect SYBR Green PCR master mix (Qiagen) on a StepOnePlus Real-Time PCR machine (Applied Biosystems ...
-
bioRxiv - Microbiology 2019Quote: ... with 0.5% NaCl at 30 °C for overnight and DNA extraction was done by DNeasy Blood and Tissue Kit (QIAGEN). Specific forward and reverse primers targeting each gene of interest were used for PCR amplification (TABLE 2) ...
-
bioRxiv - Microbiology 2019Quote: ... DNA was extracted from 0.25 to 0.5 grams of each sample using the PowerLyzer PowerSoil DNA Isolation Kit (Qiagen, US), according to the manufacturer instructions ...
-
bioRxiv - Molecular Biology 2019Quote: 1×106 MIA PaCa-2 or HAP1 cells were seeded per 6 well plate and co-transfected with 0.5 µg pCas-Guide plasmid using Effectene transfection reagent (Qiagen). 24 hrs after transfection ...
-
bioRxiv - Cell Biology 2020Quote: ... and A10 encoding constructs or 1 μg Shh constructs with or without 0.5 μg Scube2 by using Polyfect (Qiagen). Cells were grown for 2 days or 3 days for Disp−/− rescue experiments at 37°C with 5% CO2 in DMEM containing 10% fetal calf serum and penicillin-streptomycin (100 μg/mL) ...
-
bioRxiv - Genomics 2019Quote: ... Each 1 µl uracil cleavage mix contains 0.25 µl Uracil DNA Glycosylase (Enzymatics G5010) and 0.25 µl Endonuclease VIII (Enzymatics Y9080) and 0.5 µl Elution Buffer (Qiagen 19086). Unincorporated DNA oligos were digested at 37°C for 30 min using a thermocycler ...
-
bioRxiv - Microbiology 2020Quote: ... RNA was isolated from four replicate cultures for each strain grown to mid-exponential phase in THY (OD ∼ 0.5) using the RNeasy kit (Qiagen) and processed as previously described (Horstmann et al. ...
-
bioRxiv - Immunology 2021Quote: ... Samples were added to 2 ml ZR BashingBead lysis tube with 0.5 mm beads (Zymoresearch) and tubes arranged in a pre-cooled Tissuelyzer II adapter (Qiagen) and disrupted by shaking at 50 Hz for 25 min ...
-
bioRxiv - Microbiology 2022Quote: ... The nucleases were then denatured by simultaneously adding 20 μl of 0.5 M EDTA and 57 μL of 20 mg/μL Proteinase K (Qiagen) and incubating at 56°C for 2 hr ...
-
bioRxiv - Microbiology 2023Quote: Total microbial DNA was isolated from 0.2–0.5 g faecal material using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Germany) using the QIAcube (Qiagen ...
-
bioRxiv - Molecular Biology 2023Quote: ... The kidneys were homogenized using 0.5 M acetic acid and two 5-mm steel beads in TissueLyser LT (Qiagen), followed by addition of pepsin to 0.1 mg/ml and incubation for 3 days ...
-
bioRxiv - Microbiology 2023Quote: ... total RNA from WT or Pcu-shc::Δshc strains at early exponential phase (OD600 = 0.5-0.8) was extracted using a RNeasy® Mini Kit (Qiagen) according to the manufacturer’s recommended protocol ...
-
bioRxiv - Immunology 2022Quote: ... 2 μL of cDNA was added to 19 μL first round PCR reaction mix containing 0.5 U HotStarTaq Polymerase (Qiagen), 0.18 μM of each 3’ reverse primer ...
-
bioRxiv - Microbiology 2023Quote: ... DNA was extracted from sediment and electrode material (0.5 g per sample) using the DNeasy PowerLyzer PowerSoil Kit (Qiagen) following the manufacturers’ instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Total cellular DNA was extracted from approximately 0.5 to 5 million cells using a QIAamp DNA Blood Minikit (Qiagen). PCR assays were performed with 200 ng samples of DNA ...
-
bioRxiv - Immunology 2020Quote: ... 5 U/ml dispase (Stemcell) and 50 mg/ml DNase I mix (Qiagen) in complete RPMI1640 medium ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 µg/mL DNase I (AppliChem) and 200 µg/mL RNase A (Qiagen) using 4 cycles of 15 s sonification at 30% duty cycle and 30% output control ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were lysed (1.28 M sucrose, 40 mM Tris-HCl [pH 7.5], 20 mM MgCl2, and 4% Triton X-100; Qiagen) and digested (800 mM guanidine–HCl ...
-
bioRxiv - Neuroscience 2021Quote: ... The titer of the individual lentiviral solution was quantified by Lenti-X p24 Rapid Titer Kit (Qiagen, 632200) at the first round of production and then Lenti-X GoStix Plus Kit (Qiagen ...
-
bioRxiv - Bioengineering 2019Quote: Genomic DNA was isolated from ~3.7 x 106 transfected HEK293T cells using the DNeasy Blood & Tissue kit (Qiagen) following the protocol for cultured animal cells with the addition of RNase A ...
-
bioRxiv - Genetics 2021Quote: Total RNA was extracted from an aliquot of 3 x 106 cells using the RNeasy Plus Kit (Qiagen). cDNA was synthesized from extracted RNA using the iScript cDNA Synthesis Kit (BioRad ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1.28 M sucrose, 40 mM Tris-Cl, pH 7.5, 20 mM MgCl2, and 4% Triton X-100; QIAGEN) and then digested by digestion buffer (QIAGEN buffer G2 ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were lysed (1.28 M sucrose, 40 mM Tris-HCl [pH 7.5], 20 mM MgCl2, and 4% Triton X-100; Qiagen) and digested (800 mM guanidine–HCl ...
-
bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from 1 x 106 transduced HEK293T cells with the RNeasy Plus Mini Kit (Qiagen, #74134) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: RNA was extracted from 5 x 106 T cells using the RNeasy® Mini Kit (Qiagen, Cat. 74106). RNA extraction was performed following the instructions from the “Purification of Total RNA from Animal Cells Using Spin Technology” protocol given in the RNeasy® Mini Handbook ...
-
bioRxiv - Immunology 2023Quote: RNA was purified from at least 5 x 104 CD4+ T cells using the RNeasy Micro kit (Qiagen). cDNA was synthesized using the SuperScriptTMVILOTMcDNA synthesis kit (Invitrogen) ...
-
bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from 1 x 108 trypanosome cells using the Qiagen RNeasy Mini Kit (Qiagen, Netherlands) following the manufacturer’s instructions ...