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Citations for Qiagen :
451 - 500 of 958 citations for IL 4 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... DNA of the mouse pool (MP) was isolated using a QIAamp PowerFecal Pro DNA Kit (Qiagen).
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bioRxiv - Microbiology 2019Quote: Bacterial DNA extraction from mouse gut was performed with a QIAamp DNA Stool Mini Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... mouse vagal ganglia were removed and placed in sterile cell lysis buffer (Qiagen Puregene, Hilden Germany) with 0.5% SDS and 100ug/ml Proteinase K at 55°C overnight ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted from mouse or human whole blood using RNA blood mini kit (Qiagen). Isolated RNA was converted to complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from cultured human and mouse cells using an RNeasy Mini Kit (Qiagen) and included an on-column DNase treatment to eliminate contaminating genomic DNA ...
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bioRxiv - Biochemistry 2021Quote: Genomic DNA was extracted from mouse kidneys using the DNeasy Blood & Tissue Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... Quantitative real-time-PCR was performed using RT2 Profiler PCR Array for Mouse Mitochondria (330231, Qiagen) with PowerUp SYBR Green (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from mouse carotid artery or HAECs using RNeasy Plus Micro Kit (QIAGEN, 74134) and 200 ng of total RNA was subjected to reverse transcription reactions using iScript™ Reverse Transcription Supermix (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... total RNA was isolated from mouse left ventricles using the Qiagen AllPrep Kit (Qiagen, Valencia, CA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: Mitochondrial DNA and genomic DNA were isolated from mouse hearts using DNeasy Blood & Tissue Kits (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... mouse organ lysates and human primary cell supernatant samples were prepared in RNeasy Mini Kit (Qiagen) lysis buffer RLT (400μl) ...
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12-Lipoxygenase Governs the Innate Immune Pathogenesis of Islet Inflammation and Autoimmune DiabetesbioRxiv - Immunology 2021Quote: RNA was isolated from zebrafish or from mouse tissue using the RNeasy Plus Micro Kit (Qiagen) and was used to prepare cDNA using a commercial kit (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: RNA was isolated from whole mouse lung tissue using the RNeasy Spin column kit (Qiagen #75144) then reverse transcribed using the cDNA Reverse Transcription Kit (Applied Biosystems #4308228 ...
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bioRxiv - Neuroscience 2022Quote: ... Genomic DNA was isolated from Mouse Embryonic fibroblast cells (MEF) using QIAmp DNA mini kit (Qiagen), PCR amplified and subsequently cloned in vector pEGFPC1 with a constitutive promoter ...
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bioRxiv - Pathology 2022Quote: ... Total RNA of mouse and human intestinal tissue was isolated using the RNeasy Mini Kit (Qiagen) and total RNA of mouse mesenteric fat was isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: Mouse fecal pellets of approximately 0.1g were homogenized and processed using the DNA PowerSoil kit (Qiagen) with the QIACube automated platform following the manufacturer’s inhibitor removal technology (IRT ...
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bioRxiv - Genetics 2023Quote: ... Mouse Ahrr and Cyp1a1 primers were generated using the PyroMark Assay Design software version 2.0 (Qiagen). Primer sequences can be found in Table S1 ...
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bioRxiv - Pathology 2023Quote: ... Microbial DNA was isolated from mouse fecal samples (QIAamp PowerFecal Pro DNA Kit; Qiagen, Hilden, Germany). At the end of the experiment ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was prepared from mouse embryonic molar tooth RNA (extracted using RNeasy Plus Micro kit, Qiagen) and cDNA constructs were inserted in TOPO II PCR-plasmids using TOPO TA Cloning kit with chemically competent cells according to manufacturer’s protocol (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... total RNA was extracted from mouse testis using the RNeasy extraction kit (74104, Qiagen, Hilden Germany) according to the manufacturer’s instruction ...
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bioRxiv - Systems Biology 2023Quote: ... RNAs were extracted from isolated mouse SAN with RNeasy Mini Kit (Qiagen, Valencia, CA, United States) and DNAse ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was isolated from mouse tail snips using DNeasy® Blood & Tissue Kit (Qiagen, Valencia, CA) and genotyped ...
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bioRxiv - Immunology 2023Quote: The RNA extraction process involved lysing mouse colonic IEC or cultured cells using RLT buffer (QIAGEN) and extracting total RNA using the RNeasy Mini Kit (QIAGEN ...
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bioRxiv - Biochemistry 2024Quote: ... PCR arrays were performed using RT2 Profiler™ PCR Array Mouse Cytokines & Chemokines (PAMM-150Z, Qiagen). Calculations were performed by a comparative method (2−ΔΔCt ...
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bioRxiv - Biophysics 2019Quote: ... Lysates were spun for a further 20 min at 40,000 g at 4 °C and resulting supernatants were incubated 1 hour with Ni2+-nitrilotriacetate (NTA) agarose beads (QIAGEN) at 4 °C on rotating wheel ...
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bioRxiv - Neuroscience 2022Quote: RNA was isolated from ∼100,000 MSNs from 4 independent differentiations per experimental group according to the manufacturer’s protocol and purified using RNeasy columns (Qiagen). A total of 72 mRNA libraries for Illumina sequencing were prepared using KAPA mRNA HyperPrep Kit (KAPA Biosystems ...
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bioRxiv - Genetics 2019Quote: ... cells (from 4 × 104 to 2.3 × 106) were pelleted at 400xg for 4 min and resuspended in 700 μL RLT buffer (Qiagen) with beta-mercaptoethanol (1:100) ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were centrifuged at 30,597xg and 4°C for 20min and the supernatant was bound to Ni-NTA Agarose resin (Qiagen), washed with 10 CV of wash buffer (50 mM HEPES-KOH pH=7.5 ...
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bioRxiv - Genetics 2019Quote: Single cells meant for RNA processing were sorted into 96-well full-skirted Eppendorf plates that were pre-chilled at 4°C and were prefilled with 10μL of lysis buffer consisting of TCL buffer (Qiagen) supplemented with 1% beta-mercaptoethanol ...
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bioRxiv - Microbiology 2019Quote: ... Cell lysates were then incubated at 4 °C for 1-1.5 hours on one ml of Ni-NTA resin (Qiagen). The resin was then loaded into columns (Biorad ...
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bioRxiv - Evolutionary Biology 2020Quote: ... n=4 females [hybrid cross] and n=5 females [conspecific cross]) using the AllPrep RNA/DNA Mini Kit (Qiagen), and was stored at −80°C until sequencing ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared using a JA-20 rotor (18,000 rpm for 30 min at 4°C) and incubated with Ni-NTA superflow resin (Qiagen) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: Tissue lysates were further homogenized and centrifuged at 10,000 g for 10 min at 4°C in microcentrifuge spin columns (QIAshredder, Qiagen) to obtain clear protein lysate ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was extracted from P15 liver tissue (n=4 biological replicates per strain) using QIAzol Lysis Reagent (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... 19 motifs and 4 differential motifs were analyzed to predict upstream regulators using Ingenuity Pathway Analysis (IPA, QIAGEN Inc.). Gene expression signatures indicative of HMO and motif abundance were defined as genes differentially expressed with abundance in the previous limma analysis(FDR q<0.05 and |Fold Change|>1.5).
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bioRxiv - Physiology 2021Quote: ... 10-50 mg tissue was homogenized (1 mM EDTA and 4 mM sodiummetabisulfite, pH 7.4) using a TissueLyser II (Qiagen). Samples where adjusted to the same weight/volume percentage by adding a volume of homogenization buffer and the NA content was measured by ELISA (BA E-5200 ...
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bioRxiv - Plant Biology 2020Quote: ... 10mM imidazole was added and incubated on rotator for overnight at 4°C in the presence of 2.5ml of Ni-NTA beads (Qiagen). The beads were washed with EB containing 6M guanidine-HCl and 0.25% Triton X-100 ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer A supplemented with 200 mM imidazole in gravity flow ...
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bioRxiv - Molecular Biology 2022Quote: His-Prs or its variants (10 µg) were bound to 4 µl of Ni-NTA Magnetic Agarose Beads (Qiagen) in 50 µl interaction buffer (50 mM Tris pH 9.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting with buffer A supplemented with 250 mM imidazole in gravity flow ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 mM GDPNP-Mg2+) were obtained at 4°C using 0.2 M Lithium nitrate and 20% PEG3350 (PEG Suite I, Qiagen) as precipitating agent ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B supplemented with 250 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... cleared by centrifugation (20 min, 20,000 × g, 4°C) and the clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer C supplemented with 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA agarose resin (QIAGEN) eluted with buffer A supplemented with 0.5 mM TCEP and 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and cleared lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Systems Biology 2022Quote: ... Ten millilitres of culture were pelleted by immediate centrifugation (5,000 × g for 3 min at 4°C) and resuspended in 5 mL of RNAlater (76106; Qiagen). Samples were stored at 4°C overnight ...
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Deconstructing the role of iron and heme in the structure of healthy human gut microbial communitiesbioRxiv - Microbiology 2022Quote: ... Two hundred fifty microliters of each sample were transferred into 96-well PowerBead Plates (Qiagen, 27500-4-EP-BP). Seven hundred fifty microliters of RLT buffer from an AllPrep DNA/RNA 96 Kit (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was isolated at 6 time points (0, 0.5, 1, 2, 4, and 8 hours) using the RNeasy Mini kit (Qiagen) according to the manufacturer’s recommended protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... 4 ng/μL RNA carrier as described by the supplier of the RNeasy® Micro kit (Qiagen, Hilden, Germany).
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bioRxiv - Genomics 2022Quote: ... samples were incubated an additional 30 minutes with 20 µL Proteinase K and 4 µL RNase A (Qiagen 19101) and subsequently eluted for DNA using DNeasy Blood and Tissue kit ...