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Citations for Qiagen :
451 - 500 of 10000+ citations for Human Mitochondrial Open Reading Frame Of The 12S rRNA c MOTS c CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... The mei-P26[1] and OreR samples were processed to cDNA using random hexamers and ribosomal sequences were depleted with sequential eukaryotic and bacterial rRNA depletion kits (Qiagen FastSelect). Illumina dual-indexed libraries were made from these cDNAs and sequenced as 2x150bp reads.
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bioRxiv - Genomics 2023Quote: The bacterial 16S rRNA V4 variable region was amplified for 5 cycles with the HotStarTaq Plus Mastermix Kit (Qiagen, Hilden, Germany). Samples were purified with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Epidemiology 2019Quote: ... each mosquito carcass was stored individually at −80 °C in ATL lysis buffer (QIAGEN) until DNA extraction ...
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bioRxiv - Genomics 2020Quote: ... were lysed overnight at 56°C in 50% (v/v) of Buffer AL (Qiagen) and 0.5 mg.ml-1 of Proteinase K (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... The samples were frozen at –80°C and ground in a TissueLyser II (Qiagen). We extracted DNA using QIAamp DNA Investigator Kit (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with Trizol for 5 min at 4°C and homogenized using TissuLyserTM (Qiagen) for 5 min at 50 Hz ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was incubated at 4 °C overnight with Ni2+-nitrilotriacetic acid resin (Qiagen) pre-equilibrated with buffer B ...
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bioRxiv - Bioengineering 2023Quote: ... dried at 37°C for 30 min and dissolved overnight in EB buffer (Qiagen). DNA was quantified by NanoDrop (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... was stored in Qiazol at −80°C for later RNA extraction (Qiagen, Hilden, Germany). DNA was extracted using the DNeasy Blood & Tissue Kit including the optional RNase A treatment (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... and tissue was homogenized at 4°C in a tissue lyser bead mill (Qiagen) for 2 min at 20 Hz ...
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bioRxiv - Biophysics 2023Quote: ... 4 °C and the supernatant was loaded onto a NiNTA column (Qiagen, Hilden, Germany) previously equilibrated with washing buffer (20 mM Tris pH 8 ...
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bioRxiv - Immunology 2023Quote: ... Larvae were euthanized at 4°C overnight and homogenized with a tissue lyser (Qiagen) at 1800 oscillations/min (30 Hz ...
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bioRxiv - Cell Biology 2021Quote: The clarified lysate was then loaded onto an open column containing pre-equilibrated Ni-NTA (Qiagen). The column was washed with 20 mM Tris-HCl ...
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bioRxiv - Genomics 2022Quote: An aliquot of 150 μL of plasma per sample was thawed on ice and centrifuged at 3000 × g for 5 min at 4 °C and smallRNA was extracted using miRNeasy Serum/Plasma Kits (Qiagen, Cat. No. 217184, Milano, Italy) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from homogenates after overnight incubation with 20 μl of proteinase K at 56°C using the QIAamp DNA Micro Kit (Qiagen, Hilden, Germany, cat. No. 56304) following the manufacturer’s protocol for tissue samples ...
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bioRxiv - Immunology 2023Quote: ... The remaining cells were pelleted by centrifugation (4000rpm, 30min, 4C) and stored at –20°C for bulk EEV extraction by endotoxin-free maxiprep (Qiagen EndoFree Maxi Kit, Cat. 12362).
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bioRxiv - Microbiology 2022Quote: ... and 12-days post infection (3 samples/group) using the RNeasy Kit (Qiagen). Samples were processed at the Baylor College of Medicine Genomic and RNA Expression Profiling Core (Houston ...
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bioRxiv - Immunology 2021Quote: ... The plasmids were extracted with 12 columns of EndoFree plasmid Maxi kit (Qiagen). To prepare the virus library ...
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bioRxiv - Plant Biology 2021Quote: ... The DNA amplicons derived from the bacterial 16S rRNA gene were extracted from the gels using the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany). After determination of the DNA concentration of each amplicon (nanodrop ...
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bioRxiv - Microbiology 2024Quote: ... Excess rRNA was depleted using QiaSeq FastSelect 5S/16S/23S depletion kit as well as the FastSelect HMR and Plant rRNA probes (Qiagen, Hilden, Germany). RNA sequencing was performed on an Illumina NovaSeq S1 PE100bp to obtain 1.6 billion reads (Illumina Inc ...
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bioRxiv - Immunology 2023Quote: ... RNA from human PBMCs was isolated using RNeasy Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... which permitted in-frame cloning of the monomer into the vector pQE30 (Qiagen). Synthetic genes encoding the concatemers were then constructed by iterative cloning of monomer into monomer ...
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bioRxiv - Genomics 2020Quote: Testis tissue was homogenized at 4°C in 1ml QIAzol Lysis Reagent (Qiagen, Hilden, Germany) together with RNase-Free Zirconium Oxide Beads (NextAdvance ...
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bioRxiv - Biophysics 2020Quote: ... 4 °C) and the supernatant was then incubated with 200 µl Ni-NTA (#30230, Qiagen) beads for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... 4 °C) and the supernatant was then loaded onto 1 ml HisTrap HP column (Qiagen) at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate was then incubated for one hour at 4 °C with Ni-NTA (Qiagen) in batch adsorption format ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was incubated for 2 h at 4 °C with Ni2+-NTA-agarose (Qiagen) (20 mg of proteins/ml of resin ...
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bioRxiv - Microbiology 2021Quote: ... and stored at −80 °C before extraction using RNeasy with on column DNase treatment (Qiagen). Ribosomal RNA was depleted using Ribozero (Illumina ...
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bioRxiv - Neuroscience 2021Quote: ... Purification of C-terminal His6-tag-labeled GluN1 and GluN1ΔM4 by Ni2+-NTA agarose (Qiagen) chromatography was performed as in (Madry et al. ...
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bioRxiv - Developmental Biology 2019Quote: ... 25 minutes incubation at 37°c was given with RNase free 2U DNase (Qiagen-79254) in RDD buffer to remove residual DNA ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatant was incubated for 1 hour at 4°C with Ni-NTA-agarose (Qiagen), washed several times with lysis buffer and the protein was eluted by incubation for 3 hours with lysis buffer containing 500 mM imidazol ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by enzyme inactivation 20 minutes at 65°C and column-based purification (Qiagen, #74204). First strand cDNA synthesis was performed with the High-Capacity-RNA-to-cDNA Kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated for 3 minutes at 56°C in a deparaffinization solution (Qiagen, 19093). A step of digestion with Proteinase K was performed followed by a treatment with DNAses ...
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bioRxiv - Neuroscience 2023Quote: ... The eluent was then incubated overnight at 4°C with Ni-NTA beads (Qiagen, 36111), which were subsequently collected ...
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bioRxiv - Immunology 2023Quote: ... Sorted Ly6G+ cells were stored at −80°C in RLT Plus Buffer (Cat# 1053393-Qiagen). Total RNA isolation was done using RNeasy Plus Mini Kit (Cat# 74134-Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: ... Cq values were normalized to the endogenous control 5S rRNA (YP00203906, Qiagen). Relative miRNA expression was determined using the 2 −ΔΔCt method (67).
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bioRxiv - Microbiology 2023Quote: ... rRNA was first depleted using the QIAseq FastSelect -5S/16S/23S (Qiagen) kit per the protocol with some modifications ...
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bioRxiv - Genetics 2022Quote: ... All samples were then prepared with QIAseq FastSelect −rRNA HMR (Qiagen, 334386) and lllumina Truseq® Stranded Total RNA Library Prep Gold (Illumina ...
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bioRxiv - Immunology 2024Quote: ... STAT2 and 18s rRNA were measured using validated gene-specific primers (QIAGEN). Primers for ACAT1 ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was collected from 12-24hr embryos per manufacturer’s specification (RNeasy kit, Qiagen). cDNA libraries were generated with the TruSeq stranded mRNA sample library kit (Illumina ...
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bioRxiv - Immunology 2019Quote: ... RNA was then extracted from these 12 samples using an RNeasy Micro kit (Qiagen). The quantity and quality of extracted RNA was first evaluated using both a nanodrop spectrophotometer and the Agilent 2100 bioanalyzer ...
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bioRxiv - Microbiology 2023Quote: ... 12 µL were reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen), as described elsewhere (14) ...
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bioRxiv - Neuroscience 2021Quote: ... astrocytes and human brain tissue using the RNeasy Mini kit (Qiagen) and reverse transcribed using the High-Capacity RNA-to-cDNA kit (ThermoFisher Scientific) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Light organs were then transferred into 180 ul of pre-heated (98°C) ATL buffer (QIAGEN) and incubated at 98°C for 15 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... incubated for 45min at 42°C and the reaction purified with 15µl of MagAttract beads (Qiagen). The labelled DNA was eluted with TE-buffer (10mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2020Quote: An RNA probe targeting c-myb was generated using one-step RT-PCR (Qiagen, Manchester, UK) using the following primers c=myb-F 5’-CCAAGTCAGGAAAACGCCACCTCG-3’ and c-myb-R 5’-GCTGTTGTTTAGCGGAGTTGGGCT-3’ and cloned into the dual promoter vector pCRII-TOPO (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... then spun at 14000K RPM for 10 minutes at 4°C to pellet debris (Qiagen, 37901). Protein concentration in the supernatant was quantified using BCA assay (Thermo Scientific ...
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bioRxiv - Biophysics 2020Quote: ... Enzyme was inactivated (65°C, 15min) prior to PCR purification of the linearized product (Qiagen, Germany). 10μg of linearized plasmid was transformed into Pichia pastoris X-33 strain by electroporation as per the manufacturers protocol (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C) and supernatant was transferred to tubes containing 30µl precleared Ni-NTA agarose beads (Qiagen) in the presence of 0.05% Tween-20 and 15mM imidazole ...
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bioRxiv - Genomics 2021Quote: Skeletal muscle previously stored at −80°C was lysed with the RLT buffer Plus buffer (Qiagen) and beta-mercaptoethanol using the TissueLyser II (Qiagen ...