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Citations for TianGen Biotech :
501 - 550 of 563 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... and the leaf samples were pooled for each group for DNA extraction using the TIANGEN DNA purification kit (TIANGEN, DP305-02). High-throughput sequencing and Bulked Sequence Analysis (BSA ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from mouse brain lysates (mixture of 3∼4 mouse brains for each sample) using RNAprep pure Tissue Kit (Tiangen, DP431). cDNA was synthesized using HiScript II 1st Strand cDNA Synthesis Kit (Vazyme ...
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bioRxiv - Cell Biology 2021Quote: ... the first batch of cells (5×106) were collected 2 days after infection using the TIANamp genomic DNA kit (Tiangen, DP304), and the second batch of cells treated with puromycin were collected at 7d ...
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bioRxiv - Genomics 2022Quote: ... on an agarose gel and were subsequently extracted using a Universal DNA purification Kit (Catalog No. DP214-02, TIANGEN, Beijing, China) and then used as a template to synthesize dsRNA with a MEGAscript RNAi Kit (Catalog No ...
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bioRxiv - Plant Biology 2022Quote: ... the young leaves of eight to ten seedlings were sampled and pooled for genomic DNA extraction using the Plant Genomic DNA Extraction Kit (DP205, TianGen, China) following the instructions provided by the supplier.
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from less than 100 mg of 10-d-old seedlings that were grown vertically on half-strength MS plates or treated with 50 μM AA or an equal volume of DMSO for 4 h in liquid MS medium using RNAprep Pure Plant Plus Kit (TIANGEN, DP441). First-strand cDNA was synthesized using a HiScript® II cDNA Synthesis Kit (Vazyme R212-02 ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from leaf and root tissues of the natural population and transgenic rapeseed using RNAsimple Kit (DP419, TIANGEN, China). The RNA was sequenced using Illumina 2500 platform (Illumina Inc ...
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bioRxiv - Neuroscience 2022Quote: ... The strains were expanded and cultured for endotoxin-free plasmid extraction with the EndoFree Maxi Plasmid Kit V2 (DP120, Tiangen, China). The constructed plasmids were stored at −20 °C for the transfection of HEK293-T cells (49).
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bioRxiv - Microbiology 2022Quote: The DNA of pure bacterial colonies was extracted by following the procedures described in the DNA extraction kit (TIANGEN Biotech, Beijing). The 16S rRNA gene was amplified using universal primers 27F (5’-AGAGTTTGATCMTGGCTCAG-3’ ...
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bioRxiv - Cancer Biology 2023Quote: The total RNAs were extracted from experimental cells when they reached 80% confluency using an RNA extraction kit (TIANGEN, Beijing, China) and then reversely transcribed into single-stranded cDNAs by a Reverse-Transcription Kit (Promega ...
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bioRxiv - Immunology 2023Quote: ... hCD36 mice below refer to homozygous hCD36 mice) and wild-type (WT) C57BL/6J were harvested for extracting total RNA using RNAprep Pure Cell / Bacteria Kit (TIANGEN, DP430). The mRNA expression of hCD36 and mCD36 was determined using GAPDH as the internal control ...
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from middle part of ear leaf at 12 DAI with the RNA extraction Kit (DP411, TIANGEN, China). RNA purity ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted from blood using TRIzol reagent in an RNAprep Pure Tissue Kit (Cat# 4992236, TIANGEN Biotech, Beijing, China). After the RNA purity and concentration were determined with Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... A paired-end library with an insert size of 350 kb was constructed using the TIANSeq Fast DNA Library Kit (Cat. no: 4992261; Tiangen, China) and then sequenced using an Illumina NovaSeq6000 sequenator (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... viral RNA was extracted from mock- or ALSV-infected cell-culture supernatants using a viral RNA extraction kit (TIANGEN, Cat# DP315), and transcribed into cDNA using the cDNA Synthesis SuperMix (TransGen ...
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bioRxiv - Immunology 2024Quote: ... The correct construction of the recombinant plasmids was verified through Sanger sequencing and extracted using an Endotoxin-Free Plasmid DNA Miniprep Kit (Tiangen, China) before using plasmids ...
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bioRxiv - Plant Biology 2024Quote: Total RNAs was extracted from 100 mg cotton seedling samples with different treatments by grinding with Plant Total RNA Purification Kit (TIANGEN, China). RNA was then treated with DNase using the DNA-free kit following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: Total RNA samples extracted from M82 and yft1 pericarp (47 dpa, BR stage) using the RNAprep pure Plant Kit (Tiangen, Beijing, China) were used as a templates in rapid amplification of cDNA ends (RACE ...
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bioRxiv - Plant Biology 2020Quote: ... AAA-CAA) within the PGEX-6P-1 plasmid was performed using the fast Site-Directed Mutagenesis Kit (Tiangen, China, Catalog No. KM101). PCR was conducted using site-specific primers (Supplemental Table S17) ...
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bioRxiv - Microbiology 2021Quote: ... of strain TC967 was extracted from 4-day-old mycelia following the protocol described in the handbook of the Plant Genomic DNA Kit (Tiangen, Beijing, China). The gDNA was sequenced by Illumina HisSeq 2500 sequencing (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from single third-instar larva of three strains (total 30 larvae of each strain) using the DNA/RNA/Protein Isolation Kit (TianGen, Beijing, China). The first-strand cDNA was synthesized with 1 μg of total RNA using a PrimeScript™ 1st Strand cDNA Synthesis Kit with gDNA Eraser (TaKaRa Biotechnology ...
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bioRxiv - Genetics 2019Quote: ... and the whole genome DNA of the patients was extracted using DNA extraction kit (product of Beijing Tiangen Biochemical Technology Co., Ltd.) and using BloodGen Midi Kit (CWBIO ...
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bioRxiv - Synthetic Biology 2021Quote: ... The incubated solution is then subjected to agarose gel electrophoresis and the correct band is extracted using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Synthetic Biology 2021Quote: ... The incubated solution is then subjected to agarose gel electrophoresis and the correct band is extracted using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Plant Biology 2020Quote: RNA was extracted according to the instructions of the RNA Isolation Kit for polyphenol- and polysaccharide-rich plants (Tiangen Biotech, Beijing, China). The reverse transcription primer was Oligo(dT)18 ...
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bioRxiv - Plant Biology 2020Quote: ... MG), 47 dpa(Breaker stage, BR) and 54 dpa(Red Ripe stage, RR) using the RNAprep pure Plant Kit (Tiangen, Beijing, China) based on the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... The cell suspension was centrifuged for 5 minutes at 12,000 rpm and cell precipitate was resuspend in 1 ml sterilizing saline used for DNA extraction with TIANamp Genomic DNA kit (TianGen Biotechnologies, Beijing). Subsequently ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA was extracted from the 2nd pair or 3rd pair of leaf counted from the top following the protocol of TIANquick Midi Purificatioin kit (TIANGEN Biotech, Beijing). The primers were listed in Table S2.
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA has been extracted from putative transformants one-month-old leaves grown on a kanamycin-containing medium using TianGen kits (TianGen BioTech, China). The quality of the extracted genomic DNA (250–350 ng/μl ...
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from peripheral blood samples collected from all the participants using the blood DNA extraction kit (TianGen Biotech, China). We performed the whole genome sequencing on Illumina NovaSeq 6000 system (150 bp paired-end reads ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was extracted from blood samples collected from the wing vein using a DNA extraction mini kit (Tiangen Biotech, Beijing, China). All sampled hens were killed by cervical dislocation immediately after oviposition and the abdominal cavity was opened ...
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bioRxiv - Plant Biology 2022Quote: ... and total mRNA was extracted using the RNA Prep Pure Plant Plus Kit according to the manufacturer’s instructions (Tiangen Biotech, Beijing, China). A strand-switching method and the cDNA-PCR Sequencing Kit (SQK-PCS109 ...
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bioRxiv - Bioengineering 2023Quote: Total RNAs were isolated from all samples with liquid nitrogen using the RNAprep Plant RNA kit (polysaccharides&polyphenolics-rich) (TIANGEN BIOTECH (BEIJING)CO. ...
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bioRxiv - Cell Biology 2023Quote: Experimental cells were subjected to total RNA isolation (using an RNA Simple Total RNA Kit, DP419, Tiangen Biotech Co., Ltd., Beijing, China). The first strand of cDNA was obtained by adding total RNA (1.5 µg ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from 1×106 THP1 cells after various treatments (see above) by using an RNAprep pure cell/bacteria kit (Tiangen Biotech 4992235) according to the manufacturer’s instructions and was quantified using NanoDrop OneC Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: CT DNA was extracted from the culture supernatant or cell lysates using the TIANamp Genomic DNA kit (Tiangen Biotech, Beijing, China; DP304). Quantitative PCR analysis targeting the CT cryptic plasmid was performed using TaqMan Gene Expression Master (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... the DNA fragments were separated by 1.0 % agarose gel electrophoresis (1 × TAE buffer) and purified from the gel with the DNA Gel Extraction Kit (TianGen Biotech., Beijing, China), then they were sub-cloned into pCE2 TA/Blunt-Zero vector (Vazyme ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA was isolated from the cecal contents using the magnetic-based soil and stool DNA extraction kit (TIANGEN®, Beijing, China). The concentration of the extracted DNA was determined using the Qubit Fluorometric Quantitation system (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: Each of experimental cell lines was subject to its total RNAs isolated by employing an RNAsimple Kit (Tiangen Biotech CO. LTD, Beijing, China). Total RNAs (1 μg ...
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bioRxiv - Genetics 2022Quote: ... The CDS was cloned into the MCS of the modified pWMB110 vector under the ubiquitin promoter by using the EasyGeno Assembly Cloning kit (TianGen Biotech Co, Beijing). The recombinant plasmid was transformed into Agrobacterium strain C58C1 (Zoman Biotech Co ...
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bioRxiv - Molecular Biology 2019Quote: Total RNAs were isolated from intermuscular fat tissue by RNAprep Pure Tissue Kit following the manufacturer’s instructions (Tiangen Biotech Co., Ltd., Beijing, China). The purity ...
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bioRxiv - Pathology 2021Quote: ... Total RNA was extracted from mycelia of transformants cultured in V8 juice medium by using an RNA-simple Total RNA Kit (Tiangen Biotech, Beijing, China). PsFYVE1 specific primers (S5 Table ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA was extracted from the leaves of T3 transgenic plants (Table S8, lines 1954-1 and 1955-3) using a plant genomic DNA Extraction Kit (Tiangen Biotech, Beijing, China). About 20 μg of DNA was successfully digested with 5 U of EcoRV and incubated at 37 °C for 24 h ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from leaves of transgenic lines and wild-type plants treated with pathogens or hormones using the RNAprep pure plant kit (TIANGEN, DP441, Beijing, China). Then ...
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bioRxiv - Microbiology 2021Quote: The genomic DNA of the B3 strain was extracted using a Bacterial Genomic DNA Purification Kit (DP302-2, Tiangen Biotech Co., Beijing, China) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted from mid-exponential cultures of strain HP1 (both the control and experimental groups) using the RNAprep Pure Bacteria Kit (Tiangen Biotech, Beijing, China) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... Cells harboring correct plasmid were cultured and pL4440-vit2 was extracted with the TIANprep Mini Plasmid Kit (Tiangen Biotech Co. Ltd., Beijing, China). Meanwhile ...
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bioRxiv - Cell Biology 2022Quote: ... The residual plasmid frame was then inserted by the synthesized combined fragment (GL-SV40pA-pTK-RF-bGHpA) using the EasyGeno Single Assembly Cloning kit (Tiangen Biotech, Beijing, China) to construct the dual-luciferase reporter plasmid (pGL-RF) ...
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bioRxiv - Microbiology 2022Quote: ... at which point 1 mL of cells were harvested and RNA samples were extracted using the RNAprep Pure Bacteria Kit (Tiangen Co., Beijing, China). Total cDNA was generated from 500 ng of total template RNA using the HiScript III All-in-one RT SuperMix Perfect for qPCR (Vazyme Co. ...
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bioRxiv - Biochemistry 2019Quote: The extraction of colonic tissues total RNA was according to the manufacture instructions by using RNAprep pure Tissue Kit (TIANGEN BIOTECH, BeiJing, China). After that ...