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Citations for Spherotech :
201 - 250 of 318 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The samples were tethered to a 0.8-μm streptavidin-coated polystyrene bead (Spherotech, Lake Forest, IL) at one end through biotin-streptavidin interactions and to the anti-digoxigenin (Roche ...
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bioRxiv - Biophysics 2021Quote: ... which contained 0.8-μm streptavidin-coated polystyrene beads (Spherotech), the buffer ...
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bioRxiv - Biophysics 2021Quote: ... Then the chambers were flushed with reference beads (2.5-2.9 µm, Spherotech, AP-25-10) and Halo-Tag (O4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Fluorescent beads (Spherotech) were then added at a volume ratio of 1:10,000 to label the Matrigel for imaging ...
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bioRxiv - Immunology 2021Quote: ... Cells were counted using Sphero AccuCount 5-5.9 μm beads according to the manufacturers protocol (Spherotech, Cat. #ACBP-50-10).
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bioRxiv - Molecular Biology 2021Quote: ... constructs were mixed with 4 μl of polystyrene beads coated with antibodies against digoxigenin (AD beads, 0.1% v/v suspension, Ø 1.76 μm, Spherotech), 10 μl of assay buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.8 μl of streptavidin-coated polystyrene beads (SA beads, 1% v/v suspension, Ø 2 μm, Spherotech) were mixed with 1 ml of assay buffer ...
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bioRxiv - Immunology 2021Quote: ... fluorescent counting beads (AccuCount Fluorescent Particles from Spherotech) were added before analysis to calculate the number of donor Pmel-1 CD8+ T cells ...
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bioRxiv - Biophysics 2021Quote: ... molecules were covalently linked to the carboxylated polystyrene beads (Spherotech) via Carbodiimide reaction (PolyLink Protein Coupling Kit ...
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bioRxiv - Biophysics 2021Quote: We used commercially provided AntiDig-coated beads (Spherotech, 2μm diameter), Neutravidin-coated bead (Spherotec ...
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bioRxiv - Immunology 2021Quote: ... serum and IL-2 starved OT1 CTLs were incubated with streptavidin polystyrene beads (Spherotech) coated with H-2Kb-OVA and ICAM-1 at a 1:1 ratio for various times at 37 ºC and immediately lysed in 2× cold lysis buffer containing phosphatase inhibitors (1 mM NaF and 0.1 mM Na3VO4 ...
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bioRxiv - Biophysics 2021Quote: ... DNA molecules were captured between two trapped streptavidin-coated polystyrene beads ( ?4.38 µm, Spherotech).
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bioRxiv - Immunology 2021Quote: ... Frequency calculation was obtained by mixing 1/10th of sample with 200,000 fluorescent beads (Spherotech) for normalization (16) ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 2 µl of 1% w/v amine polystyrene fluorescent yellow particles (NH2-beads, Spherotech, Inc) or carboxyl polystyrene fluorescent yellow particles (Spherotech ...
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bioRxiv - Synthetic Biology 2021Quote: ... or carboxyl polystyrene fluorescent yellow particles (Spherotech, Inc.) in 100 µl 1x PBS were run as a negative control.
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bioRxiv - Bioengineering 2021Quote: ... One percent (w/v) stock solutions of 1-1.4μm high-intensity polystyrene Nile red fluorescent microparticles were procured from Spherotech Inc (FH-1056-2 ...
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bioRxiv - Bioengineering 2021Quote: The fluorescent yellow microbeads (10.2 μm; Spherotech, Lake Forest, IL, USA) were incubated with Pierce protein-free blocking buffer (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: Fluorescent PMMA (PolyAn, 5 μm pink) and PS (Spherotech, 7 μm yellow) microbeads were prepared in standard solutions ...
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bioRxiv - Biophysics 2021Quote: ... The first channel of the slide contained 4.36 μm Streptavidin coated polystyrene microspheres (Spherotech, Fulda, Germany) diluted in sample buffer (40 mM Tris ...
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bioRxiv - Neuroscience 2021Quote: ... size reference beads were used to set a minimum diameter of 10um (Spherotech) for all collected cells ...
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bioRxiv - Immunology 2021Quote: ... and cell numbers were quantified using PE-conjugated fluorescent counting beads (Spherotech).
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bioRxiv - Cell Biology 2021Quote: ... to carboxy polystyrene beads (Spherotech) via EDC chemistry ...
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bioRxiv - Biophysics 2021Quote: ... Anti-digoxigenin antibodies were coupled to 1 um polystyrene beads via NHS-EDC coupling: 50 ul of 5% (w/v) 1 um carboxylated polystyrene beads (Spherotech, USA) was buffer exchanged in to reaction buffer (MES-NaOH ...
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bioRxiv - Cancer Biology 2021Quote: ... To determine absolute cell numbers AccuCount Blank Particles (Spherotech) were added to the samples ...
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bioRxiv - Biochemistry 2021Quote: ... provided by Spherotech, are composed of a core of polystyrene and CrO2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Fluorescent beads (Rainbow Calibration beads, Spherotech, RCP-30-5A) samples were also acquired during every flow cytometry experiment to enable conversion of data from arbitrary units (a.u. ...
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bioRxiv - Neuroscience 2021Quote: ... voltages were set according to optimal PMT sensitivity using the peak 2 (Spherotech) voltration technique described previously by Maeker and Trotter 35 ...
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bioRxiv - Immunology 2021Quote: Streptavidin-conjugated polystyrene beads (Spherotech) were coated with 1 μg/mL biotinylated ICAM-1 and/or various concentrations of biotinylated H-2Kb-OVA ...
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bioRxiv - Immunology 2021Quote: ... to array particles (Spherotech) following manufacturer’s protocol for direct labeling ...
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bioRxiv - Immunology 2021Quote: ... carboxy functionalized blue particle array beads (Spherotech) were directly conjugated with streptavidin (Southern Biotech ...
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bioRxiv - Cell Biology 2021Quote: ... streptavidin-coated polystyrene beads (0.56 μm in diameter, Spherotech Inc., Libertyville IL) were functionalized with biotinylated anti-5His antibodies (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... Calibration of flow cytometer was performed with the three-millimeter rainbow calibration particles (Spherotech, Libertyville, IL) before each run ...
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bioRxiv - Microbiology 2021Quote: SPHERO carboxyl magnetic blue fluorescent beads (Spherotech, USA) were coupled with SARS-CoV-2 whole spike protein (Lake Pharma ...
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bioRxiv - Neuroscience 2021Quote: ... voltages were set up according to optimal PMT sensitivity using the peak 2 (Spherotech) voltration technique described previously by Maeker and Trotter [46] ...
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bioRxiv - Immunology 2021Quote: ... Standardized SPHERO rainbow beads (Spherotech, Cat#RFP-30-5A) were used to track and adjust PMTs over time ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei counts were determined using an internal control bead population (Spherotech, Lake Forest, IL, USA) at 106 beads/mL ...
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bioRxiv - Biophysics 2021Quote: ... This reaction was then diluted 5-fold into optical tweezers assay buffer containing 0.08% w/v streptavidin-coated polystyrene beads (2.1 μm diameter, Spherotech) and incubated on ice for at least 15 minutes ...
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bioRxiv - Biophysics 2021Quote: An optical trap (Tweez305, Aresis, Slovenia) was applied to drag an r = 1.60 μm radius polystyrene bead (HUP-30-5, Spherotech, US) in a large dextran-rich condensate at 13 different velocities (Figure 2e) ...
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bioRxiv - Biophysics 2021Quote: ... streptavidin-coated polystyrene beads (Spherotech, Lake forest, IL, USA), while the beads used in MT measurements were 1.0 μm diameter ...
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bioRxiv - Biophysics 2021Quote: ... and a microsphere (Spherotech) coated with anti-digoxigenin antibody (Roche Diagnostics ...
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bioRxiv - Biophysics 2021Quote: ... we 10-fold diluted a 5% w/v stock of polystyrene beads (PP-50-10, Spherotech, Inc.) and put 5 µm onto an easy cleaned [104] microscope glass slide ...
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bioRxiv - Immunology 2021Quote: Streptavidin-coated beads with different intensities of PE-channel fluorescence (Spherotech SVFA-2558-6K and SVFB-2558-6K) were incubated with the following biotinylated antigens ...
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bioRxiv - Cancer Biology 2021Quote: ... Total number of cells in tumors and spleens were determined using SPHERO AccuCount Fluorescent Particles (Spherotech, IL). Expression of H-2Kb and PD-L1 were assayed in the CD45− fraction generated from WT and CCN4-KO YUMM1.7 tumors using the antibodies ...
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bioRxiv - Synthetic Biology 2021Quote: ... to enable conversion of GFP fluorescence into calibrated MEFL units 58 a single well per plate contained 15 μL of 8-peak Rainbow Calibration Particles (Spherotech, RCP-30-5A) diluted into 200 μL PBS ...
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bioRxiv - Immunology 2021Quote: ... The cells were screened in a 30 min time-course assay for secretion of antibodies that bound to 7 µm streptavidin beads (Spherotech) coated with 10 µg/mL SARS-CoV-2 spike or RBD ...
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bioRxiv - Molecular Biology 2021Quote: ... Laminar flow separated channels 1-3 were used to form a single-stranded biotin-λ DNA tether as follows: a single 4.38-µm streptavidin-coated polystyrene bead (Spherotech) was caught in each trap in channel 1 (trap stiffness of ∼0.4 pN/nm) ...
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bioRxiv - Microbiology 2021Quote: ... Nile Red nominally 5 µm (5-7.9 µm) (Spherotech, FP-6056-2, 1.5 × 108/ml) and Nile Red nominally 10 µm (10-14 µm ...
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bioRxiv - Microbiology 2021Quote: ... and Nile Red nominally 10 µm (10-14 µm) (Spherotech, FH-10056-2, 107 microspheres/ml) with unmodified surfaces ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... counting beads (Spherotech; Lake Forest, IL), BSA (Rockland Immunochemicals ...
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bioRxiv - Immunology 2021Quote: ... and following surface staining cells were fixed and permeabilized according to the manufacturer’s instructions using the BD Cytofix/Cytoperm Plus Kit (BD. For counting the cells AccuCount Fluorecent particles (Spherotech, Lake Forest, IL, USA) were used ...