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Citations for Spherotech :
1 - 50 of 318 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... No. PP-10-10, 5% w/v, 1.23 µm mean diameter, ∼4.89 × 1010 particles/mL, obtained from Spherotech, Illinois USA), beads were diluted to 108 particles/mL in PBS prior to the addition of virus ...
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bioRxiv - Cancer Biology 2024Quote: Tethers were pulled from the plasma membrane using streptavidin-coated polystyrene beads with a mean diameter of 1.87 μm (SVP-15, Spherotech). Right before the experiments ...
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bioRxiv - Biophysics 2024Quote: ... The ∼3.3 kb construct was attached to 800-nm-diameter streptavidin-coated polystyrene beads (Spherotech) with a 1-hour room temperature and stored in 1x phosphate-buffered saline (PBS ...
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bioRxiv - Biophysics 2024Quote: ... Streptavidin coated 440 nm polystyrene beads (Spherotech, Lake Forest IL) were functionalized with biotinylated α-penta-His antibody (Qiagen ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The tethered flies were positioned on top of a polypropylene ball (Ø: 6 mm; Spherotech GmbH, Fulda, Germany) using a 3D-micromanipulator ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 µm anti-Digoxigenin coated polystyrene beads (Spherotech) were trapped with a 1064 nm ...
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bioRxiv - Cell Biology 2024Quote: ... Voltages were established using relevant controls and were maintained (+/- 10%) across replicates using calibration particles (Spherotech, URCP-38-2K). Cells were separated from debris by optical gating based off forward and side scatter area ...
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bioRxiv - Synthetic Biology 2024Quote: ... UltraRainbow Calibration Particles (Spherotech #URCP-100-2H) were run with each flow cytometry experiment ...
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bioRxiv - Immunology 2024Quote: ... and XBB.1.5 strains of SARS-CoV-2 were incubated with different fluorescence intensity peaks of the SPHERO Streptavidin fluorescent Yellow Particles (Spherotech, Cat. #SVF3-2552) at 20 ng per μg beads for 30 min at room temperature on an end-over-end mixer ...
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bioRxiv - Neuroscience 2024Quote: ... voltages were setup according to optimal PMT sensitivity using the peak 2 (Spherotech) voltration technique described previously by Maeker and Trotter64 ...
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bioRxiv - Immunology 2024Quote: ... Frequency calculation was obtained by mixing 1/10th of samples with 200,000 fluorescent beads (Spherotech) for normalization.
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bioRxiv - Microbiology 2024Quote: ... Compensation was calculated by FACS DIVA software 6.1.3 using appropriate single antibody labeled compensation beads from SpheroTech. Data analysis was performed using FlowJo software v9.6 (Treestar).
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bioRxiv - Molecular Biology 2024Quote: ... using two polystyrene microspheres coated with streptavidin (Spherotech, 2.1 μm diameter) and held by two optical traps according to the method presented in [15,16] ...
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bioRxiv - Bioengineering 2024Quote: ... Streptavidin-coated beads (nominal diameter 5.14 μm) were purchased from Spherotech, Inc ...
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bioRxiv - Immunology 2024Quote: ... Avidin beads (Spherotech, SVP-30-5) were pre-incubated in the same medium with different concentrations of CD4- or CD8-biotin and washed once ...
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bioRxiv - Biophysics 2024Quote: ... was tethered between 4.38-µm SPHERO Streptavidin Coated polystyrene beads (Spherotech, Cat. No. SVP-40-5) in Trap1 and 2.12-µm SPHERO Anti-Dig polystyrene beads (Spherotech ...
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bioRxiv - Biophysics 2024Quote: ... in Trap1 and 2.12-µm SPHERO Anti-Dig polystyrene beads (Spherotech, Cat. No. DIGP-20-2) in Trap2 ...
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bioRxiv - Cell Biology 2024Quote: All beads were provided by Spherotech (US) unless stated otherwise ...
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bioRxiv - Synthetic Biology 2024Quote: ... Fluorescence calibration beads (Spherotech, part no. RCP-30-20A) were also measured with each flow cytometry plate to enable calibration of the flow cytometry data to molecules of equivalent fluorophore5–7.
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bioRxiv - Immunology 2024Quote: Recombinant biotinylated XBB.1.5 spike protein was incubated with SPHERO Streptavidin fluorescent Yellow Particles (Spherotech, Cat. #SVF3- 2552) at 20 ng per μg beads for 30 min at room temperature on an end-over-end mixer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Calibration beads (Spherotech RCP305A) were run routinely to ensure that experiments from different days were comparable ...
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bioRxiv - Cell Biology 2024Quote: Glutathione-functionalized beads with a diameter of 2 µm (Spherotech, GSHP-20-5, Lake Forest, IL) were sonicated for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... SPHERO Amino Polystyrene beads ranging from 8.0 to 12.9 µm in diameter (Spherotech, #AP-100-10) were labelled with amine labeling as detailed below for cells ...
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bioRxiv - Cell Biology 2024Quote: ... we used readily fluorescent (Nile Red) polystyrene beads ranging from 5.0 to 7.9 µm in diameter (Spherotech, #FP-6056-2). The beads were FACS sorted using BD Biosciences FACS Aria ...
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bioRxiv - Cell Biology 2024Quote: ... Carboxylated polystyrene microspheres (3% v/v, Spherotech) of 4.67 um diameter were washed (2000g for 1 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Registration information was obtained using 3μm diameter fluorescence beads (Ultra-rainbow, URFP-30-2, Spherotech) suspended in aqueous 4% agarose gel ...
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bioRxiv - Developmental Biology 2024Quote: ... This was achieved by mechanically breaking the Matrigel dome (with organoids within) with pipettes followed by Liberase (CF-2058-2, Spherotech, Inc.) incubation at 37°C for 15 min to achieve enzymatic dissociation ...
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bioRxiv - Developmental Biology 2024Quote: ... Carboxyl Fluorescent Pink Particles (CF-2058-2, Spherotech, Inc.) was used at 1:10 dilution ...
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bioRxiv - Biophysics 2024Quote: ... solution is passed through the chamber and left 50 min to react followed by reference beads (2.5-2.9 µM, Spherotech, AP-25-10) and O4 ligand (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... sub-micron polystyrene size-standard beads (BDSORP beads, Spherotech, #NPPS-4K) were used to calibrate before each sorting experiment ...
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bioRxiv - Immunology 2024Quote: ... Sphero AccuCount Beads (ACBP-50-10) were used for counting in-vitro cultured cells following instructions from the manufacturer (Spherotech, Inc). Mitochondrial membrane potential was measured using MitoTracker™ Deep Red FM dye and MitoTracker Orange CM-H2TMRo dye (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Spherobeads (Spherotech Cat#SRCP-35-2A) and OneComp beads (Invitrogen Cat#01-1111-42 ...
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bioRxiv - Immunology 2024Quote: Recombinant HA protein and BSA were incubated for 30 min on ice with different fluorescence intensity peaks of the 7K and 8K Blue Particle Array Kit (Spherotech) at 40 µg/mL ...
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bioRxiv - Immunology 2024Quote: ... Laser voltages were set with the assistance of SPHERO Supra Rainbow particles (Spherotech, Inc., #SRCP-35-2A) based on settings optimized during the development of this antibody panel ...
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bioRxiv - Immunology 2024Quote: ... SPHERO™ AccuCount Particles (Spherotech, Inc., Lake Forest, IL, USA) were added to the samples during acquisition for cell quantification ...
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bioRxiv - Immunology 2024Quote: Custom cytometric arrays were generated as previously described42 using 4 µm and 5 µm carboxy functionalized array kits (Spherotech, PAK-4067-8K and PAK-5067-10K). Streptavidin (SA ...
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bioRxiv - Immunology 2024Quote: Multiplex streptavidin (SA) beads (PAK-5067-10K; Spherotech) were coated with biotinylated MHC-I (Kd ...
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bioRxiv - Immunology 2024Quote: ... Cell counts of LP and skin populations were calculated using AccuCount (Spherotech) counting beads that were spiked into the sample prior to acquisition ...
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bioRxiv - Neuroscience 2024Quote: ... at 10μg/mL.20 million avidin coated polystyrene particles (1.7-2.2 μm, 0.1% w/v, Spherotech Inc, VFP-2052-5) were added and incubated with biotinylated antibody for 1h at room temperature on rotator covered by aluminum foil ...
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bioRxiv - Neuroscience 2024Quote: The spherical treadmill was based on a polypropylene ball (PP ball, 6mm in diameter, Spherotech GmbH, Germany) supported by a custom-built ball holder (Electronics workshop ...
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bioRxiv - Neuroscience 2024Quote: ... to microbeads through an avidin-biotin bond was modified based on the commercial protocol (Spherotech Inc. ...
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bioRxiv - Biophysics 2024Quote: ... The construct was attached to the 1.76 μm streptavidin coated polystyrene bead (1% w/v, Spherotech, USA) using laminar flow of the microfluidic device (Fig ...
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bioRxiv - Microbiology 2024Quote: ... Channel 1 contained 4.38 µm streptavidin-coated polystyrene beads (Spherotech). Channel 2 contained the DNA substrate harboring 3x parS sequences or the control DNA without parS both of them labeled with multiple biotins at both ends ...
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bioRxiv - Molecular Biology 2024Quote: ... utilizing two polystyrene microspheres coated with streptavidin (Spherotech, 2.1 μm diameter) held by two optical traps ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Deep red fluorescent labelled polystyrene (PS) particles (SkyBlue particles, 100-300 nm, catalog number #FP-0270-2, batch number #AL01) were purchased from Spherotech and provided as non-sterile 0.25% (w/v ...
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bioRxiv - Biophysics 2024Quote: ... 1 µL 0.5% w/v streptavidin coated 2.1 µm diameter polystyrene beads (Spherotech, Cat# SVP-20-5) were diluted into 1 mL assay buffer ...
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bioRxiv - Biophysics 2024Quote: ... were combined with 4 µL 0.1% w/v 2.1 µm diameter polystyrene beads coated with anti-digoxigenin antibodies (Spherotech, Cat# DIGP-20-2), diluted to 10 µL with assay buffer (10 mM MOPS ...
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bioRxiv - Genomics 2024Quote: ... Instrument was calibrated using 23.9 um beads (Spherotech, ACURFP2.5-250-5). Single hepatocytes were sorted into 384-well plates (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... and 1 uL of 2 um anti-digoxigenin coated polystyrene particles (Spherotech #DIGP-20-2) for 10 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... 2 uL of 4 um streptavidin coated polystyrene particles (Spherotech #SVP-40-5) were also diluted in 500 uL C-trap buffer ...