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Citations for Spherotech :
1 - 50 of 311 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: All beads were provided by Spherotech (US) unless stated otherwise ...
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bioRxiv - Synthetic Biology 2024Quote: ... Fluorescence calibration beads (Spherotech, part no. RCP-30-20A) were also measured with each flow cytometry plate to enable calibration of the flow cytometry data to molecules of equivalent fluorophore5–7.
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FACS-Sortable Picoreactors for Ultra High-throughput Screening of Catalysts in Biphasic EnvironmentsbioRxiv - Bioengineering 2024Quote: ... Laser delays were manually calibrated with 32 µm AccuCount Ultra Rainbow beads (Spherotech). Forward and side scattering voltage were manually optimized samples of double emulsions ...
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bioRxiv - Immunology 2024Quote: Recombinant biotinylated XBB.1.5 spike protein was incubated with SPHERO Streptavidin fluorescent Yellow Particles (Spherotech, Cat. #SVF3- 2552) at 20 ng per μg beads for 30 min at room temperature on an end-over-end mixer ...
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bioRxiv - Synthetic Biology 2024Quote: ... UltraRainbow Calibration Particles (Spherotech #URCP-100-2H) were run with each flow cytometry experiment ...
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bioRxiv - Immunology 2024Quote: ... and XBB.1.5 strains of SARS-CoV-2 were incubated with different fluorescence intensity peaks of the SPHERO Streptavidin fluorescent Yellow Particles (Spherotech, Cat. #SVF3-2552) at 20 ng per μg beads for 30 min at room temperature on an end-over-end mixer ...
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bioRxiv - Neuroscience 2024Quote: ... voltages were setup according to optimal PMT sensitivity using the peak 2 (Spherotech) voltration technique described previously by Maeker and Trotter64 ...
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bioRxiv - Microbiology 2024Quote: ... No. PP-10-10, 5% w/v, 1.23 µm mean diameter, ∼4.89 × 1010 particles/mL, obtained from Spherotech, Illinois USA), beads were diluted to 108 particles/mL in PBS prior to the addition of virus ...
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bioRxiv - Cancer Biology 2024Quote: Tethers were pulled from the plasma membrane using streptavidin-coated polystyrene beads with a mean diameter of 1.87 μm (SVP-15, Spherotech). Right before the experiments ...
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bioRxiv - Biophysics 2024Quote: ... The ∼3.3 kb construct was attached to 800-nm-diameter streptavidin-coated polystyrene beads (Spherotech) with a 1-hour room temperature and stored in 1x phosphate-buffered saline (PBS ...
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bioRxiv - Biophysics 2024Quote: ... Streptavidin coated 440 nm polystyrene beads (Spherotech, Lake Forest IL) were functionalized with biotinylated α-penta-His antibody (Qiagen ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The tethered flies were positioned on top of a polypropylene ball (Ø: 6 mm; Spherotech GmbH, Fulda, Germany) using a 3D-micromanipulator ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 µm anti-Digoxigenin coated polystyrene beads (Spherotech) were trapped with a 1064 nm ...
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bioRxiv - Cell Biology 2024Quote: ... Voltages were established using relevant controls and were maintained (+/- 10%) across replicates using calibration particles (Spherotech, URCP-38-2K). Cells were separated from debris by optical gating based off forward and side scatter area ...
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bioRxiv - Immunology 2024Quote: ... Frequency calculation was obtained by mixing 1/10th of samples with 200,000 fluorescent beads (Spherotech) for normalization.
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bioRxiv - Microbiology 2024Quote: ... Compensation was calculated by FACS DIVA software 6.1.3 using appropriate single antibody labeled compensation beads from SpheroTech. Data analysis was performed using FlowJo software v9.6 (Treestar).
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bioRxiv - Molecular Biology 2024Quote: ... using two polystyrene microspheres coated with streptavidin (Spherotech, 2.1 μm diameter) and held by two optical traps according to the method presented in [15,16] ...
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bioRxiv - Bioengineering 2024Quote: ... Streptavidin-coated beads (nominal diameter 5.14 μm) were purchased from Spherotech, Inc ...
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bioRxiv - Immunology 2024Quote: ... Avidin beads (Spherotech, SVP-30-5) were pre-incubated in the same medium with different concentrations of CD4- or CD8-biotin and washed once ...
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bioRxiv - Biophysics 2024Quote: ... was tethered between 4.38-µm SPHERO Streptavidin Coated polystyrene beads (Spherotech, Cat. No. SVP-40-5) in Trap1 and 2.12-µm SPHERO Anti-Dig polystyrene beads (Spherotech ...
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bioRxiv - Biophysics 2024Quote: ... in Trap1 and 2.12-µm SPHERO Anti-Dig polystyrene beads (Spherotech, Cat. No. DIGP-20-2) in Trap2 ...
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bioRxiv - Cell Biology 2023Quote: Fluorescent beads experiments: 10–14 µm polystyrene beads (SPHERO™, Spherotech, Inc., Lake Forest, IL, USA) were embedded in 20 µl fibrin gels (10µl thrombin ...
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bioRxiv - Biophysics 2023Quote: ... 1% DMSO (Helicon) followed by addition of 10 μl streptavidin-coated microspheres (2.1 μm) (Spherotech, Cat № SVP-20-5). After washing away the unbound microspheres ...
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bioRxiv - Bioengineering 2023Quote: ... a known quantity of ∼5 micron AccuCount Fluorescent Beads (Spherotech, Lake Forest, IL, USA) was used for CHO MP counting.
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bioRxiv - Bioengineering 2023Quote: ... size gates were constructed using fluorescent calibration beads from Spherotech (Lake Forest, IL, USA). For experiments using the BD FACSAriaII flow cytometer ...
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bioRxiv - Immunology 2023Quote: ... with the addition of 1x104 counting beads (#ACBP-100-10, Spherotech) being acquiring data on a BD LSR Fortessa X-20 (BD Bioscience ...
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bioRxiv - Biophysics 2023Quote: ... the zero-suction pressure was found by aspirating a polystyrene bead (3.43 μm, Spherotech) into the pipette and reducing the suction pressure until the bead stopped moving ...
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bioRxiv - Cell Biology 2023Quote: ... All fluorescent polystyrene microbeads were purchased from Spherotech Inc ...
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bioRxiv - Cell Biology 2023Quote: ... an aliquot of the crosslinked protein-DNA mixture was mixed with 5 μL 2.1 μm diameter anti-digoxigenin antibody coated polystyrene beads (Spherotech) and incubated at room temperature for 15 min ...
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bioRxiv - Biophysics 2023Quote: ... which were used to form DNA tethers between two 3.13-μm streptavidin-coated polystyrene beads (Spherotech) held in traps with a stiffness of ∼0.6 pN/nm ...
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bioRxiv - Biophysics 2023Quote: ... Measurements were performed using a dumbbell conformation: the protein of interest was attached in between two 1 μm-sized silica beads (Spherotech, US), using double stranded (ds ...
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bioRxiv - Bioengineering 2023Quote: ... Pre-rinsed 4.5 µm streptavidin labeled polystyrene particles (Spherotech SVP-40-5) are combined with 1 µM biotinylated oligoes that are a reverse complement to the oligo covalently attached to the slide and incubated for 20 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... biotinylated flagellin peptides were conjugated on to Streptavidin coated 4um polystyrene beads (Spherotech, Inc. Cat # PAK-4067-8K). Beads conjugated with each individual peptide have different fluorescent intensity in the APC/Cy7 channel so that they can be differentiated from each other by flow cytometry ...
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bioRxiv - Neuroscience 2023Quote: ... Laser voltages were set with the assistance of SPHERO Supra Rainbow particles (Spherotech, Inc., #SRCP-35-2A) based on settings optimized during the development of this antibody panel ...
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bioRxiv - Synthetic Biology 2023Quote: ... 8-peak beads (Spherotech, RCP-30-5A) were used to establish how fluorescence relates to standardized units of molecules of equivalent fluorophores (MEFL ...
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bioRxiv - Biophysics 2023Quote: ... Individual DNA molecules were trapped between two 1.76 μm diameter streptavidin-coated polystyrene beads (Spherotech) initially injected into a separate channel.
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bioRxiv - Molecular Biology 2023Quote: ... Amino-modified polystyrene beads (AP-30-10) with a nominal diameter of 3.4 µm were purchased from Spherotech Inc ...
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bioRxiv - Cell Biology 2023Quote: ... We then incubated each sample with 0.22-μm light yellow beads (Spherotech, FP-0245-2) resuspended in 2× SSC as fiducial markers for drift correction and washed the sample with 2× SSC briefly.
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bioRxiv - Bioengineering 2023Quote: ... soluble SiRPα-Fc at saturating concentrations were immobilized on 0.4-0.6um Protein G Yellow Fluorescent Particles (PGFP-0552-5, Spherotech) at 4°C for 1h ...
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bioRxiv - Neuroscience 2023Quote: ... Voltages were set up according to optimal PMT sensitive using the peak 2 (Spherotech) voltration technique ...
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bioRxiv - Biophysics 2023Quote: ... the chamber was washed with non-magnetic beads (Spherotech, AP-25-10) followed by Halo-Tag amine (O4 ...
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bioRxiv - Biochemistry 2023Quote: ... as compared with standard polystyrene beads of 880 nm and 1350 nm size from the SPHEROTM Nano Fluorescent particle standard kit (Spherotech, Lake Forest, IL).37 Marker-positive platelet and microparticle count were expressed as % of all events captured in a joint gate “Platelets + Microparticles” ...
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bioRxiv - Biochemistry 2023Quote: ... which were used to form DNA tethers between 4.89-μm streptavidin-coated polystyrene beads (Spherotech) held in traps with a stiffness of ~0.3 pN/nm ...
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bioRxiv - Immunology 2023Quote: ... Cells were counted using Sphero AccuCount 5-5.9 μm beads according to the manufacturer’s protocol (Spherotech, Cat. #ACBP-50-10).
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bioRxiv - Biophysics 2023Quote: ... Streptavidin-coated polystyrene beads (Spherotech, SVP-10-5) was used as reference beads for the stage drift correction ...
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bioRxiv - Biophysics 2023Quote: Streptavidin-coated polystyrene particles (4.34 µm diameter) were ordered from Spherotech Inc ...
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bioRxiv - Biophysics 2023Quote: ... Aminated polystyrene beads (6 μm) were purchased from Spherotech (# AP-60-10). Artificial saliva was purchased from Fisher Scientific (# NC1873815) ...
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bioRxiv - Immunology 2023Quote: ... counting beads (Spherotech) were added prior to running flow cytometry ...
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bioRxiv - Developmental Biology 2023Quote: ... the procedure was the same as described above despite that His-beads were substituted by Streptavidin Magnetic Particles (Spherotech).
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bioRxiv - Microbiology 2023Quote: ... cells were counted by flow cytometry using spike-in of a known amount of AccuCount Blank Particles 5-5.9 µm (Spherotech, ACBP-50-10) as previously described7.