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Citations for Avanti Polar Lipids :
901 - 946 of 946 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... by extrusion using a Mini Extruder fitted with a polycarbonate membrane with 1 µm pore size (Avanti Polar Lipids) in binding buffer without magnesium (50 mM HEPES/KOH ...
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bioRxiv - Systems Biology 2020Quote: ... and 50 μL of the internal standard C12 ceramide (d18:1/12:0) (Avanti Polar Lipids, Alabaster, AL, USA) to samples with continuous shaking overnight (12 hours ...
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bioRxiv - Neuroscience 2023Quote: A concentration of either 1 mg/mL Total Ganglioside Extract or 1mg/mL POPS was used (Avanti Polar Lipids). In brief ...
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bioRxiv - Cell Biology 2024Quote: A 50 μL methanol solution containing 20 pmol each of C15:0/18:1 PA (Avanti Polar Lipids, #330721) and C12:0/13:0 PE (Avanti Polar Lipids ...
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bioRxiv - Microbiology 2020Quote: ... Total lipid was then separated by 2D-HPTLC spiked with 1 μg PA(C17:0/C17:0) (Avanti Polar lipids) using chloroform/methanol/28% NH4OH ...
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bioRxiv - Biophysics 2020Quote: ... functional MgtE at high density in phospholipid vesicles consisting of a 10:1 mixture of egg phosphatidylcholine (Avanti Polar Lipids) and the adjuvant lipid A (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... Cer d18:1/C17:0 [4.5 ng/μl], GluCer d18:1/C12:0 [8.3 ng/μl] and GM1 [87 ng/μl], Avanti Polar Lipids) was added ...
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bioRxiv - Biochemistry 2020Quote: ... All lipid internal standards used are listed in Table 1 internal standards (all from Avanti Polar Lipids, Alabaster, AL, USA).
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bioRxiv - Microbiology 2022Quote: ... solution containing internal standards (16:0 D31_18:1 phosphocholine, C17:0 cholesteryl ester, stigmasterol and N-dodecanoylsphingosylphosphorylcholine, 0.2 nmol each, from Avanti Polar Lipids) were added to 3105cell ...
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bioRxiv - Neuroscience 2024Quote: Mature microglia were exposed to 10 μM GalCer (C8 Galactosyl(β) Ceramide (d18:1/8:0)) (Avanti Polar Lipids, 860538P) for 16 ...
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bioRxiv - Microbiology 2020Quote: ... The suspension was extruded through polycarbonate membranes with 1 or 0.2 µm pore size using a mini-extruder (Avanti Polar Lipids) to create large or small unilamellar vesicles (LUV/SUV) ...
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bioRxiv - Cell Biology 2022Quote: ... Peptide fractions were pooled then incubated at a molar ratio of 1:1.1 (excess lipid) with MPB-PE (Avanti Polar Lipids) for 3- 12 hours at 25C with end-over-end mixing ...
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bioRxiv - Biochemistry 2023Quote: ... Ceramidase activity was assessed by incubating 1.8 μM of purified SIDT1 and SIDT2 proteins with 40 μM ceramide (d18:1/18:0) (Cat# 860518P, Avanti Polar Lipids) at room temperature for 30 minutes ...
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A gated hydrophobic funnel within BAX binds long-chain alkenals to potentiate pro-apoptotic functionbioRxiv - Cell Biology 2024Quote: Hexadecanal (16:0 aldehyde) was purchased as 1 mg/ml (4.16 mM) in methylene chloride solution (Cat. No. 857458M, Avanti Polar Lipids), aliquoted into 25 μl ...
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bioRxiv - Biophysics 2021Quote: ... functional ChRmine at high density into phospholipid vesicles consisting of a 10:1 mixture of chicken egg yolk phosphatidylcholine (egg PC; Avanti Polar Lipids) and the adjuvant lipid A (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots were subjected to acidic Bligh and Dyer lipid extractions in the presence of internal lipid standards added from a master mix containing 40 pmol d7-PC mix (15:0/18:1-d7, Avanti Polar Lipids), 25 pmol PI (17:0/20:4 ...
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bioRxiv - Biochemistry 2021Quote: ... A proteoliposome antigen was prepared by reconstituting purified functional OxlT at high density into phospholipid vesicles consisting of a 10:1 mixture of egg phosphatidylcholine (PC) (Avanti Polar Lipids) and adjuvant lipid A (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... Aliquots corresponding to 1500-2000 pmol total lipid were subjected to acidic Bligh and Dyer extractions performed in the presence of internal lipid standards from a master mix containing 40 pmol d7-PC mix (15:0/18:1-d7, Avanti Polar Lipids), 25 pmol PI (17:0/20:4 ...
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bioRxiv - Cell Biology 2022Quote: ... The used master mix of lipid internal standards contained 50 pmol d7-PC mix (15:0/18:1-d7, Avanti Polar Lipids), 25 pmol PI (17:0/20:4 ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was separated from cell debris and supplemented with NP-40 (EDM Biosciences) to a final concentration of 1% and DHPC (Avanti Polar lipids) to a final concentration of 30 mM ...
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bioRxiv - Molecular Biology 2022Quote: ... a proteoliposome antigen was prepared by reconstituting purified hZnT7 at high density into phospholipid vesicles consisting of a 10:1 mixture of chicken egg yolk phosphatidylcholine (egg PC; Avanti Polar Lipids) and adjuvant lipid A (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... lipid mixtures were created by combining DSPC and DSPE-PEG2000 at a 9:1 molar ratio (Avanti Polar Lipids, Alabaster, AL, USA) in a solution that also included propylene glycol at a concentration of 15% (v/v) ...
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bioRxiv - Plant Biology 2024Quote: ... D-glucosyl-ß-1,1′-N-palmitoyl-D-erythro-sphingosine (d18:1/16:0 - GluCer or Glucosylceramide) were purchased from Avanti Polar Lipids. Sphingolipids were dissolved in ethanol (100%) ...
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bioRxiv - Cell Biology 2024Quote: ... Lipids were extracted by adding 990 µl of chloroform/methanol 17:1 (v/v) and internal standards (127 pmol ceramide 12:0 and 124 pmol sphingomyelin 12:0; Avanti Polar Lipids), followed by shaking at 900 rpm/min in a ThermoMixer (Eppendorf ...
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bioRxiv - Molecular Biology 2024Quote: ... Internal standard mixture (SPLASH® LIPIDOMIX® and Cer 18:1;O2/17:0, both from Avanti Polar Lipids, Alabaster, AL, USA) were added to the samples ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mL of snail serum was spiked with a known volume of SPLASH Lipidomix II mass spectrometry standard (Avanti Polar Lipids, #330709) and then extracted according to the method described above ...
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bioRxiv - Evolutionary Biology 2022Quote: ... lipid 1 in Table S1) was not commercially available during this project and therefore was synthesized de novo by Avanti Polar Lipids based on a previously reported procedure [60] with slight modifications starting with commercially available (R)-Solketal ...
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bioRxiv - Bioengineering 2024Quote: ... DMG-PEG (MW 2000) (DMG-PEG2000) (Cat. No. 880151P) and 18:1 PA (Cat. No. 840875P) were purchased from Avanti Polar Lipids. Cholesterol (Cat ...
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bioRxiv - Microbiology 2024Quote: ... the final lipid solution (20 mg ml-1) was extruded at least ten times through a Mini Extruder (Avanti Polar Lipids, USA) equipped with polycarbonate membranes of 0.1 μm pore size ...
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bioRxiv - Developmental Biology 2020Quote: ... The supernatant (S2) was mixed with 1/8 volume of 10% NP-40 and 300 mM DHPC (reconstituted in lysis buffer; Avanti Polar Lipids 850306P) and incubated for 10 min on ice ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µL of 100 µM Palmitate-2,2-D2 (Eurisotop: DLM-1153-0)/ 1 x 10E6 cells For lipids (SPLASH LIPIDOMIX, Avanti Polar Lipids, 330707-1EA) 10 μl/sample) ...
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Antigen Geometry Tunes Mast Cell Signaling Through Distinct FcεRI Aggregation and Structural ChangesbioRxiv - Biophysics 2023Quote: ... 18:1 (Δ9-Cis) PC (DOPC) and 16:0 DNP Cap PE lipids were purchased from Avanti Polar Lipids (850375C and 810528C, respectively). DNP-Peg12-NHS ester was purchased from BroadPharm (BP-22397) ...
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bioRxiv - Microbiology 2023Quote: ... lyso sphingomyelin (LSM) d18:1 (d9) and palmitoyl-L-Carnitine (CAR) 16:0 (d3) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). Arachidonic acid (AA ...
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bioRxiv - Biophysics 2024Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (18:1 Liss Rhod PE) in chloroform form were purchased from Avanti polar lipids (USA). The A375 cell line was purchased from ATCC (USA).
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bioRxiv - Biophysics 2020Quote: ... as well as the in the sn-1 chain per-deuterated versions POPC-d31 and POPG-d31 were purchased as powder from Avanti Polar Lipids (Alabaster, AL), Cholesterol (Chol ...
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bioRxiv - Neuroscience 2024Quote: ... Each cell pellet was resuspended in 500 µL of resuspension buffer (0.05 M HCl, 49% Methanol, 1% SPLASH Lipidomic Mass Spec Standard [Avanti Polar Lipids, Cat. Nu. 330707]), and 1 mL methyl-tert-butyl-ether was added ...
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bioRxiv - Pathology 2023Quote: ... exact mass peaks corresponding to H+ adducts from standards of C18:1 (Δ-cis) carnitine oleoyl L-carnitine (Avanti Polar Lipids, Inc., Alabaster, AL) and 16:0-18:1 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (Avanti Polar Lipids ...
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bioRxiv - Immunology 2023Quote: ... PE-D31 16:0/18:1 and TG-D5 19:0/12:0/19:0 (Product # 860374, 850367, 8609040 and 830456 from Avanti Polar lipids, Alabama, USA) were added ...
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bioRxiv - Neuroscience 2023Quote: ... and Acyl-CoA (18:0, 870718P, Avanti Polar Lipids Inc.; 16:0, 870716P, Avanti Polar Lipids Inc.; 24:1, 870725P, Avanti Polar Lipids Inc.) for reaction were water-bath sonicated prior to the reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μL of a customised isotope labelled internal standard lipid mixture and 1 μL of a d5-TG Internal Standard Mixture I (Avanti Polar Lipids, Alabaster, AL, USA) were added ...
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bioRxiv - Biophysics 2023Quote: ... Small unilamellar vesicles are prepared by drying a mixture of 93% POPC and 7% 18:1 biotinyl-PE (Avanti Polar Lipids, catalog #850457C and 870282C) under compressed nitrogen gas followed by overnight drying under vacuum ...
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Identification of ABHD6 as a regulator of lysophosphatidylserines in the mammalian liver and kidneysbioRxiv - Biochemistry 2024Quote: ... 20 μg of the soluble or membrane proteomic fraction obtained from mammalian tissues or cells was incubated with 100 μM lyso-PS substrate (C17:1 lyso-PS; Avanti Polar Lipids, catalog no: 858141) in DPBS to a final volume of 100 μL at 37 °C with constant shaking (Eppendorf ...
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bioRxiv - Physiology 2024Quote: ... The homogenate was mixed with 1 mL of Extraction Buffer containing IPA/H2O/Ethyl Acetate (30:10:60, v/v/v) and Avanti Lipidomix Internal Standard (diluted 1:1000) (Avanti Polar Lipids, Inc. Alabaster, AL). Samples were vortexed and transferred to bead mill tubes for homogenization using a VWR Bead Mill at 6000 g for 30 seconds ...
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bioRxiv - Physiology 2024Quote: ... The homogenate was mixed with 1 mL of Extraction Buffer containing IPA/H2O/Ethyl Acetate (30:10:60, v/v/v) and Avanti Lipidomix Internal Standard (diluted 1:1000) (Avanti Polar Lipids, Inc. Alabaster, AL). Samples were vortexed and transferred to bead mill tubes for homogenization using a VWR Bead Mill at 6000 g for 30 seconds ...
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bioRxiv - Physiology 2024Quote: ... Plasma was mixed with 1 mL of Extraction Buffer containing IPA/H2O/Ethyl Acetate (30:10:60, v/v/v) and Avanti Lipidomix Internal Standard (diluted 1:1000) (Avanti Polar Lipids, Inc. Alabaster, AL). Samples were vortexed and transferred to bead mill tubes for homogenization using a VWR Bead Mill at 6000 g for 30 seconds ...
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bioRxiv - Molecular Biology 2022Quote: ... USA) was added to all test samples before lipid extraction (10 μL of a 1:10 dilution of Avanti polar lipids to 10 μL of neat plasma) for normalisation of raw peak areas and to correct for differences in extraction(11).