Labshake search
Citations for Covaris :
751 - 800 of 1249 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... The DNA was transferred to an AFA microTUBE (Covaris 520045) and fragmented in a Covaris E220 using duty factor 10 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Then chromatin was sheared with the E220 sonicator (Covaris) and chromatin concentration was measured with the Qubit 3.0 (Thermo Fischer Scientific) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Purified DNA was sheared using the Covaris S220 ultrasonicator with microTUBE AFA (Covaris, cat# 520045). Methylated and non-methylated DNA fragments were separated using the EpiXplore Methylated DNA Enrichment Kit (Clontech ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were washed two times with PBS and then sonicated for 40 min (S220 focused- ultrasonicator, Covaris, Woburn, MA). After removal of cell debris ...
-
bioRxiv - Molecular Biology 2021Quote: ... 3µg of genomic DNA was diluted in RNase-Free water and transferred to a AFA Fiber Crimp-Cap (PN520052, Covaris) microtube ...
-
bioRxiv - Molecular Biology 2021Quote: ... DNA was sheered using a Covaris S220 (Covaris). 3µg of genomic DNA was diluted in RNase-Free water and transferred to a AFA Fiber Crimp-Cap (PN520052 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 μg of high molecular weight gDNA from each sample (n=20–22 per pool) was sheared using a g-TUBE to ~15 kb (Covaris). Sheared gDNA samples were then used as input for SMRTbell preparation using the Express Template Preparation Kit 2.0 ...
-
bioRxiv - Immunology 2021Quote: ... cDNA was brought to a final volume of 15 μl and sheared using a LE220 focused-ultrasonicator (Covaris, Woburn, MA) with the following shearing parameters ...
-
bioRxiv - Molecular Biology 2021Quote: ... Following DNA sonication (Covaris S220) and Ampure XP size fractionation to generate DNA fragments of 100-300 bp ...
-
bioRxiv - Molecular Biology 2021Quote: ... Lysates were sonicated by Covaris Sonicator to ~500 bp fragments (130 μL/tube ...
-
bioRxiv - Molecular Biology 2021Quote: ... sonicated (Covaris S220), and amplified using biotin (MEF ...
-
bioRxiv - Physiology 2021Quote: ... livers were placed in tissueTUBEs (Covaris) and snap-frozen in liquid nitrogen before cryofracture using the CP02 cryoPREP Automated Dry pulverizer (Covaris ...
-
bioRxiv - Genomics 2021Quote: ... Chromatin was fragmented in microTUBEs with the E220 (Covaris) using the low cell shearing protocol (Duty cycle 2% ...
-
bioRxiv - Genomics 2021Quote: Metagenomic libraries were prepared by shearing 100 ng of DNA to 550 bp long products using the Covaris LE220 (Covaris) and size selected with SPRI beads (Beckman Coulter) ...
-
bioRxiv - Genetics 2021Quote: ... DNA was then fragmented to 90 to 250 bp by sonication using a Covaris E220 Evolution Sonicator (Covaris, Woburn, MA, USA), with a target peak of around 130bp as determined by Agilent Technologies 2100 Bioanalyzer using a High Sensitivity DNA chip ...
-
bioRxiv - Genetics 2021Quote: ... SMRTbell libraries were constructed from genomic DNA samples sheared to between 10 and 20 kb using the G-tubes protocol (Covaris; Woburn, MA, USA), end repaired ...
-
bioRxiv - Genetics 2021Quote: ... We opted for DNA fragmentation as one component of DNA degradation and used the adaptive focused acoustics technology (Covaris), which has the advantage of fragmenting DNA to a preset average fragment length ...
-
bioRxiv - Genetics 2021Quote: ... samples were sonicated (Covaris, E220). Sonicated lysate was then diluted 1:10 with ChIP dilution buffer (0.01% SDS ...
-
bioRxiv - Genetics 2021Quote: ... Crosslinked chromatin was fragmented to 150-300 bp using a Covaris S220 focused-ultrasonicator device (Covaris). 10 μg of chromatin was immunoprecipitated with the one of the following antibodies ...
-
bioRxiv - Genomics 2021Quote: ... Mechanical DNA shearing was performed with the Covaris S220 through microTube-50 AFA Fiber Screw-Cap (Covaris®) using a setting of 30% duty factor ...
-
bioRxiv - Genomics 2021Quote: ... HMW-gDNA was then fragmented to the target size of ∼400bp (Covaris, S2 and E220). Fragments were then purified with PureLink PCR Purification kit (ThermoFisher) ...
-
bioRxiv - Genomics 2021Quote: ... Genomic DNA was fragmented either through BamHI digestion (Methods) or sheared to 10 kbp using g-tubes (Covaris), following manufacturers” instructions ...
-
bioRxiv - Genomics 2021Quote: About 500 ng of amplified DNA were sheared by sonication (Covaris LE220) to generate a median size of 400 bp ...
-
bioRxiv - Genomics 2021Quote: ... DNA (1.5μg) was sonicated using a Covaris E220 sonicator (Covaris, Woburn, MA, USA). Fragments were end-repaired ...
-
bioRxiv - Genomics 2021Quote: ... using a g-Tube (Covaris). The sheared gDNA was end-repaired using polishing enzymes ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... One μg depleted RNA was fragmented (Ultrasonicator system S220, Covaris; 175 W ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... For each sample the DNA of pooled female and male flies was extracted using a high salt extraction protocol [58] and sheared with a Covaris S2 device (Covaris, Inc. Woburn, MA, USA). Libraries were prepared using the TruSeq DNA PCR-Free protocol (Illumina ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... A total of 400 ng of DNA was sonicated with a Covaris S2 (Covaris, Woburn, MA) to produce fragments ∼150-350 bp in length prior to library preparation ...
-
bioRxiv - Genomics 2021Quote: ... 100 ng of DNA was fragmented to an average size of 350 bp by ultrasonication (Covaris ME220 ultrasonicator). DNA sequencing libraries were prepared using dual-index adapters with the TruSeq Nano DNA Library Prep kit (Illumina ...
-
bioRxiv - Genomics 2021Quote: ... 120 μL hydrated DNA was transferred to a Covaris microtube (AFA fiber pre-slit snap cap, 6×16 mm) and sheared using a probe sonicator (Covaris S220 focused ultrasonicator instrument ...
-
The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... were sonicated on a Covaris ME220 sonicator (Catalog # 500295, Covaris, Inc.) using following settings ...
-
bioRxiv - Developmental Biology 2021Quote: ... Nuclei were prepared from cells according to Covaris truChIP™ Chromatin shearing kit protocol and sonicated to fragments (average fragment length: 300–500 bp) using M220 focused Ultrasonicator (Covaris, Wolburn, MA). Chromatin (10 uL ...
-
The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... around 30 brains in 110µl lysis buffer in one microtube-130 AFA Fiber Pre-Slit Snap-Cap (Catalog #520045, Covaris, Inc. Woburn, MA) were sonicated on a Covaris ME220 sonicator (Catalog # 500295 ...
-
bioRxiv - Genomics 2021Quote: ... we used truChIP Chromatin Shearing Kit (Covaris Inc., MA, USA) following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2021Quote: Chromatin was extracted and sheared from ~120 mg human hippocampus using truChIP Chromatin Shearing Kit (Covaris Inc., MA, USA) following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2021Quote: ... protein–DNA crosslinks were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
-
bioRxiv - Genomics 2021Quote: ... protein–DNA cross-links were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
-
bioRxiv - Genomics 2021Quote: ... Cells were fixed in 1% formaldehyde and sonicated using the truChIP Chromatin Shearing Kit (Covaris) on the ME220 Focused-Ultrasonicator (Covaris ...
-
bioRxiv - Genomics 2021Quote: ... Beads were then washed thrice with Shearing Buffer D3 (Covaris), once with High Salt Washing Buffer (50mM HEPES pH 7.5 ...
-
bioRxiv - Genomics 2021Quote: ... on the ME220 Focused-Ultrasonicator (Covaris) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2021Quote: ... Lysates were sonicated (E210 Focused-ultrasonicator, Covaris) and the DNA was sheared to an average length of ∼200-500 bp ...
-
bioRxiv - Genomics 2021Quote: ... fresh-frozen VP tissues from 12-week-old mice were pulverized (Cryoprep Impactor, Covaris), resuspended in PBS + 1% formaldehyde ...
-
bioRxiv - Immunology 2021Quote: ... Fragmentation of gDNA was carried out via Covaris S2 (Covaris), at duty cycle 10% ...
-
bioRxiv - Plant Biology 2021Quote: ... 5 µg of genomic DNA was fragmented using a Covaris E220 (Covaris, Inc. Woburn, MA, USA) with the following settings ...
-
bioRxiv - Bioengineering 2021Quote: ... 100 ng of DNA was sheared to 500 bp using a focused ultrasonicator (Covaris). The sheared DNA fragments were size selected using SPRI beads (Beckman Coulter) ...
-
bioRxiv - Bioengineering 2021Quote: ... All samples were stored in low TE buffer (10 mM Tris-HCl, 0.1 mM EDTA, pH 8) and were fragmented by Covaris ultrasonicator to have a mean size of 150 bp except cfDNA ...
-
bioRxiv - Biochemistry 2021Quote: ... the DNA was fragmented by sonication (Covaris S2) to fragment sizes ranging from 220–250 bp ...
-
bioRxiv - Neuroscience 2021Quote: ... The cells were then prepared for sonication using the truChIP chromatin shearing kit (Covaris Cat# 520154). Briefly ...
-
bioRxiv - Neuroscience 2021Quote: ... Nuclei were then transferred to 1mL milliTUBE with AFA fiber (Covaris Cat# 520130) and sonicated using the E220 focused-ultrasonicator (Covaris Cat# 500239) ...
-
bioRxiv - Neuroscience 2021Quote: ... and sonicated using the E220 focused-ultrasonicator (Covaris Cat# 500239). Chromatin immunoprecipitation was then performed on the sheared DNA using the iDeal ChIP-seq kit for transcription factors (Diagenode Cat# C01010170) ...