Labshake search
Citations for Covaris :
501 - 550 of 558 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... DNA was then fragmented to 90 to 250 bp by sonication using a Covaris E220 Evolution Sonicator (Covaris, Woburn, MA, USA), with a target peak of around 130bp as determined by Agilent Technologies 2100 Bioanalyzer using a High Sensitivity DNA chip ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was sheared to a mean size of 600 bp using a Covaris LE220 focused ultrasonicator (Covaris Inc., Woburn, MA). DNA fragments were Ampure (Beckman Coulter Inc. ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 µg of DNA per sample was sheared to the average fragment size of 160 bp using a Covaris S220 sonicator (Covaris, #500217). DNA quality was validated using the TapeStation 4200 system (Agilent ...
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bioRxiv - Genetics 2022Quote: ... Genomic DNA was shared to a size of approximately 300-400 bp by a Covaris Focused-ultrasonicator M220 (Covaris, LLC., USA). DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: ... 1μg genomic DNA was sheared using a Covaris M220 Focused Ultrasonicator in a 130μL microTUBE AFA fiber snap-cap vial (Covaris, Cat#: 520045). The following parameters were used to gain 500bp insert libraries ...
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bioRxiv - Microbiology 2020Quote: ... and 500 ng of input DNA was sheared into ∼550 bp fragments using a LE220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). Library preparation was performed using the TruSeq PCR-free Library Prep Kit (Illumina) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Samples with DNA fragment lengths longer than 500 base pairs (bp) were sonicated on a Covaris M220 (Covaris, Wobum, MA, USA) for 45 seconds at 50 W peak power and a duty factor of 20% ...
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bioRxiv - Genomics 2021Quote: DNA (1.5 μg) was sonicated to a 100–1500-bp size range using a Covaris E220 sonicator (Covaris, Woburn, MA, USA). The fragments were end-repaired and 3′-adenylated ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was sheared to an average fragment size of 400 bp using a Covaris M220 ultrasonicator (Covaris, Woburn, MA, USA). Libraries were prepared using a protocol largely based on (Rowan et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA from ChIP and input fractions was further sheared to an average size of approximately 150 bp by sonication (Covaris M220). Samples were then prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Genomics 2022Quote: High-molecular-weight DNA was isolated from frozen cell pellets using a modified Gentra Puregene method and sheared using gTUBE (Covaris, Inc.) to 20 kb mode size ...
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bioRxiv - Genomics 2022Quote: ... DNA (1.5μg) was sonicated to a 100- to 1500-bp size range using a Covaris E220 sonicator (Covaris, Woburn, MA, USA). Fragments were end-repaired ...
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bioRxiv - Cancer Biology 2023Quote: ... We utilized 20-50ng of cfDNA and 100ng of matched germline DNA (gDNA) that was sonicated to reduce the fragment size to 180-220bp (Covaris M220). Libraries were sequenced on the Illumina HiSeq2500 platform by the Next Generation Sequencing Facility at the University of Trento (Italy) ...
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bioRxiv - Genomics 2023Quote: ... Human FFPE genomic DNA from lung tumor tissue was fragmented to 200 bp average size using a Covaris S2 (Covaris Inc.) with the following settings ...
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bioRxiv - Genomics 2023Quote: ... An aliquot of 200 ng input in 50 ul total was used to perform DNA fragmentation (shearing) with Covaris LE220 (Covaris Inc.) method to a target of 300 bp fragments ...
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bioRxiv - Genomics 2023Quote: The DNA for short read sequencing was sheared to 550 bp on a Covaris M220 focused-ultrasonicator (Covaris, Woburn, MA, USA) by the University of Wisconsin-Madison Biotechnology Center (Madison ...
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bioRxiv - Systems Biology 2023Quote: ... the extracted genomic DNA was digested into 400–550 bp fragments by using the M220 Focused-ultrasonicator (Covaris Ltd, Brighton, UK). The sequencing library was constructed using the Nextera XT DNA Library LT kit (Illumina ...
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bioRxiv - Genomics 2023Quote: ... We quantified the purified DNA with a Qubit instrument (Thermo Fischer Scientific) to shear 330 ng of DNA into fragments of ∼ 300 bp with a Covaris sonicator (Covaris Inc.) and ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA was sheared to an average fragment size of 350 bp with a Covaris M220 Focused-ultrasonicator (Covaris, Woburn, Massachusetts, USA). We assessed the DNA fragment size distribution using a High Sensitivity DNA ScreenTape on a 4150 TapeStation System (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Half of the DNA was then sheared into 20 kb fragments with a COVARIS g-TUBE (Covaris, Inc., Woburn, MA, USA), centrifuging at 4,200 rpm for 1 minute to increase DNA throughput ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was resuspended in water and sheared into 3-5 kb fragments using the S220 Focused-ultrasonicator (Covaris Inc., MA, USA).
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bioRxiv - Genomics 2020Quote: ... The purified DNA was sheared to a size of 250-450 bp by sonication using a Covaris M220 instrument (Covaris, Woburn, MA) with peak power 50 W ...
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bioRxiv - Microbiology 2019Quote: A total of 60 ng of genomic DNA from each sample was sheared to an average size of 350 bp using a Covaris M220 Focused-ultrasonicator (Covaris, Woburn, MA) with Micro AFA fiber tubes (Covaris ...
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bioRxiv - Plant Biology 2019Quote: ... 5 µg of genomic DNA was fragmented by acoustic shearing to a target value of 250 bp using a Covaris S220 (Covaris, Woburn, MA), according to manufacturer’s recommendations ...
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bioRxiv - Genomics 2020Quote: ... The DNA was sheared to a mean fragment length of 20-30 kb with Covaris g-TUBEs (Covaris Inc., Woburn, MA, USA). The length of the DNA was measured with a TapeStation (4200 ...
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bioRxiv - Genomics 2020Quote: ... two micrograms of high molecular weight genomic DNA from each sample was sheared using g0-tube to ∼8 Kb (Covaris, Woburn, MA). These sheared gDNA samples were size selected to include 5 Kb-9 Kb fragments with Blue Pippin (Sage Science ...
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bioRxiv - Cell Biology 2021Quote: ... Each was brought to 5% SDS by volume (~1 ml total final volume) and subjected to complete lysis and DNA shearing using a Covaris Model S220 Focused Acoustic device (Covaris, Woburn MA) in 1ml glass Covaris vials ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA (5-10 µg) samples were sheared to an average size of 20 kbp via G-tube (Covaris; Woburn, MA, USA), end repaired and ligated to hairpin barcoded adapters prior to sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... Isolated genomic DNA was mechanically sheared to an average size of 250 bases using a Covaris instrument LE220 (Covaris Inc., Woburn, MA). The fragmented DNA was enzymatically repaired and end-modified with adenosine (NEBNext® Ultra™ II End Repair/dA-Tailing Module ...
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bioRxiv - Cancer Biology 2020Quote: Library Preparation: 450 nanograms of Genomic DNA from each sample was fragmented by Adaptive Focused Acoustics (E220 Focused Ultrasonicator, Covaris, Woburn, Massachusetts) to produce an average fragment size of 350 basepairs (bp) ...
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bioRxiv - Genomics 2019Quote: ... we fragmented the genomic DNA of all the tissue samples to an average size of 100-300 bp via sonication (Covaris, Massachusetts, USA). The genomic fragments were end repaired and TrueSeq-methylated adaptors were ligated to their ends ...
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bioRxiv - Genetics 2020Quote: ... DNA was sheared to 200 bp to 850 bp (average size = 400 bp) using the Covaris S2 instrument (Covaris, Woburn, MA, USA). DNA was then concentrated to a volume of 50 µL using the DNA Clean & Concentrator kit (Cat ...
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bioRxiv - Genomics 2022Quote: ... For the paired end library a total of 2 µg of DNA was sheared targeting 1 kbp fragments on a Covaris-S2 (Covaris Brighton, UK), size selected on a Sage Science Blue Pippin 1.5% cassette (Sage Science ...
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bioRxiv - Microbiology 2022Quote: ... genomic DNA from each sample was sheared to an average size of 350 bp using a Covaris M220 Focused-ultrasonicator (Covaris, Woburn, MA) with Micro AFA fiber tubes (Covaris ...
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bioRxiv - Plant Biology 2023Quote: ... Three flow cells were loaded with libraries constructed from DNA sheared to 20 Kbp using a G-tube (Covaris, Woburn, Massachusetts, USA). Reads were deposited in the ENA under project number PRJEB37025 ...
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bioRxiv - Genomics 2023Quote: ... of extracted genomic DNA (gDNA) were fragmented to a median size of ∼300 base pairs using a Covaris ME220 ultrasonic shearing system (Covaris, Woburn, MA). For samples with a DNA integrity number (DIN ...
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bioRxiv - Genomics 2024Quote: ... a dilution of 5 µg HMW DNA in 120 µL elution buffer was transferred to a g-tube (Covaris Part No. 520079) for 6 passes of centrifugation with 1,990 x g for 2 min ...
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bioRxiv - Plant Biology 2023Quote: ... genomic DNA was fragmented to an average size of 500 bp using an M220 Focused-ultrasonicator M220 (Covaris Inc., Woburn, MA. USA). A paired-end library with insert sizes ranging from 450 to 550 bp was constructed using the TruSeq DNA PCR-Free Library Prep kit (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... tumour tissue and organoid DNA were fragmented to 200 base pair (bp) length using a Covaris E Series instrument (Covaris, MA, USA), while cfDNA was not further fragmented ...
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bioRxiv - Systems Biology 2019Quote: Pure genomic DNA (approx. 1000 ng) was fragmented to a mean size of 200-300 bp using the Covaris S220 System (Covaris, Woburn, Massachusetts, USA). Then ...
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bioRxiv - Genomics 2019Quote: ... we prepared the library using 10 μg genomic DNA that was sheared to a size of 20kb fragments by g-tubes (Covaris, Inc., Woburn, MA) according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2021Quote: ... SMRTbell libraries were constructed from genomic DNA samples sheared to between 10 and 20 kb using the G-tubes protocol (Covaris; Woburn, MA, USA), end repaired ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA crosslinks were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Plant Biology 2021Quote: A total amount of 5.2 µg genomic DNA spiked with 26 ng lambda DNA was fragmented by sonication to lengths of 200-400 bp with a Covaris S220 (Covaris, Woburn, MA, USA), followed by end-repair and adenylation ...
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bioRxiv - Genomics 2019Quote: ... 5 µg of the genomic DNA extracted from adipose or lung tissues of randomly selected mice (n = 2) were sheared with an S2 ultrasonicator (Covaris, Woburn, MA, USA) in 10 mM Tris buffer (pH 8.0 ...
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bioRxiv - Genetics 2020Quote: ... a SMRTbell library was constructed from a genomic DNA sample sheared to an average size of ~10 kb using the G-tubes protocol (Covaris, Woburn, MA, USA). Single strands were removed ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: A total of 1 – 10 ng genomic DNA was fragmented by shearing to an average fragment size of 300 bp using an Adaptive Focused Acoustics sonicator (Covaris, Woburn, MA, USA). After purification ...
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bioRxiv - Genomics 2020Quote: Ion proton shotgun sequencing metavirome DNA (approx. 1000 ng) was fragmented to a mean size of 200-300 bp using the Covaris S220 System (Covaris, Woburn, Massachusetts, USA). Then ...
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bioRxiv - Genomics 2021Quote: ... STK005 SMRTbell libraries were constructed from genomic DNA samples sheared to between 10 and 20 kb using the G-tubes protocol (Covaris; Woburn, MA, USA), end repaired ...
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bioRxiv - Genomics 2020Quote: ... genomic DNA was fragmented to 100-600 bp with peak size of 350-400 bp using an LE220 Ultrasonicator (Covaris, Woburn, MA, USA). For FS PCR-free libraries ...