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Citations for Novus Biologicals :
1351 - 1389 of 1389 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cells were simultaneously incubated with 100 μM A-769662 (or DMSO) and 1 mg/mL β1-integrin antibody clone K20 (Novus Biologicals) for the indicated amount of time (2-5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... The frozen sections or fixed cells were treated with the following primary antibodies: anti-BrdU (rat, NB500-169, Novus Biologicals, 1:2000); anti-βIII-tubulin (mouse ...
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bioRxiv - Molecular Biology 2020Quote: ... then the PVDF membrane was incubated together with HRP-conjugated secondary antibody (HRP-linked anti-rabbit IgG antibody, 1:3000, cat. no. NB710-57836; Novus Biologicals, LLC) at room temperature for 1.5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... All antibodies were diluted 1:50 in PBS containing 5% (v/v) normal goat serum (NGS; 100 μl/coverslip) (Novus Biologicals, UK) and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... the neurons were stained with primary chicken polyclonal antibody against the 160 kDa fragment of neurofilament (1:500) (Novus Biologicals; NB300-222) and the secondary antibody of AlexaFluor 488 goat anti-chicken IgY (1:400 ...
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bioRxiv - Cell Biology 2022Quote: ... These membranes were blocked in 6% nonfat milk for 1.5 h at room temperature and then probed with antibodies against survivin (cat. no. NB500-201, Novus Biologicals; 1:200), cyclin D1 (cat ...
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bioRxiv - Immunology 2021Quote: ... IHC on mouse bones was performed on deparaffinized and rehydrated sections with unconjugated primary antibodies against F4/80 (clone Cl:A3-1; Novus Biological, CO, USA), osteocalcin (polyclonal ...
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bioRxiv - Microbiology 2023Quote: ... Sections were then incubated with primary antibodies diluted in 0.1% triton-X containing tris-buffered saline (TBS): rabbit anti-SARS-CoV-2 nucleocapsid protein (Novus Biologicals; 1:100) and goat anti-human angiotensin converting enzyme 2 (ACE2 ...
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bioRxiv - Physiology 2024Quote: ... Aortic sections were incubated with goat anti-α-smooth muscle actin (1:500 dilution; Novus Biologicals, Centennial, CO, USA; Cat#: NB300-978), rabbit anti-smooth muscle myosin heavy chain 11 (1:500 dilution ...
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bioRxiv - Immunology 2024Quote: ... washed with wash buffer (5 mg/mL BSA in PBS) was mixed with 1 µg Hif1a Ab (Novus Biologicals, NB-100-105) for 4 hrs at 4℃ rotating ...
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bioRxiv - Cancer Biology 2023Quote: ... and stable polyclonal cell lines were selected with 1 μg/ml puromycin and maintained in their respective media with tetracycline-free 10% FBS (Novus Biologicals #S10350) and 1 μg/ml puromycin ...
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bioRxiv - Neuroscience 2023Quote: ... SARS-CoV-2 N protein was visualized using an Alexa Fluor® 488 conjugated rabbit monoclonal anti-SARS-CoV-2 nucleocapsid antibody at a 1:1000 concentration (NBP2-90988AF488; Novus Biologicals). SARS-CoV-2 spike protein was visualized using a mouse monoclonal anti-SARS-CoV-2 spike antibody at a 1:1000 concentration (GTX632604 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then blocked with 5% skim milk and incubated with 1 μg/μL rabbit BMAL1 antibody (Catalog: NB100- 2288, RRID: AB_10000794, Novus Biologicals, Littleton, CO) and 1:2000 rabbit β-ACTIN antibody (Catalog ...
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bioRxiv - Immunology 2023Quote: ... γ-H2AX staining was performed in PBT buffer in a 1:10,000 dilution (gamma H2AX Ser139, Rabbit, Novus Biologicals, Englewood, CO, USA) and incubated overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... membrane transfer and immunoblotting were performed as previously described (50) using the following antibodies: anti-53BP1 (1:2000, Novus Biologicals #NBP2-54753SS), anti-ATM (1:1000 ...
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bioRxiv - Bioengineering 2021Quote: ... Microcarriers were incubated overnight at 4°C in blocking buffer with rabbit anti-human type VI collagen and rabbit anti-human type XII collagen primary antibodies (each 1:200, Novus Biologicals, Littleton, CO). Microcarriers were washed with PBS and incubated in goat anti-rabbit Fluorescein-conjugated secondary antibody (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... IHC was conducted using rabbit polyclonal antibodies raised against the SARS-CoV nucleocapsid protein (clone NB100-56576 [1:100], Novus Biologicals, Littleton, CO) and biotinylated anti-rabbit IgG ...
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bioRxiv - Cell Biology 2021Quote: ... Immunohistochemistry (IHC) was conducted on consecutive paraffin-embedded sections using the primary antibodies against HIF1α (rabbit polyclonal, Novus Biological, NB100-479, 1:500), SF3B1 (rabbit monoclonal ...
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: ... Sections were then blocked with normal goat serum and incubated at room temperature with a primary antibody against CA-IX (1:500, Novus Biologicals, Cambridge, UK) or HIF1α (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were then incubated overnight at 4°C in a 1:100 dilution of primary antibody (anti-MPP10, Novus Biologicals, NBP1-84341; anti-Fibrillarin, EMD Millipore, MABE1154; anti-POLR1A, Novus Biologicals, NBP2-56122 ...
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bioRxiv - Cell Biology 2022Quote: ... Rat monoclonal anti-MNR were used (1/500; Chevrier et al, 2016) for Microtubule displacement assays while anti MNR (Novus Biological NBP1-90929) for expansion microscopy ...
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bioRxiv - Developmental Biology 2021Quote: ... and non-specific staining was blocked using 10% fetal bovine serum/neonatal goat serum for 1 hr prior 90 min incubation with either biotinylated anti-laminin antibody (Novus Biologicals, Colorado, USA) or unconjugated primary antibody against IBA1 (FUJIFILM Wako Chemicals ...
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bioRxiv - Molecular Biology 2023Quote: ... The blots were blocked in TBS containing 5% non-fat milk for 30 mins and incubated with SARS-CoV-2 nucleocapsid protein antibody (1:1000, NB100-56576, Novus Biological, CO, USA) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Chips were then incubated with fluorescent antibodies against CD81 (CD81-CF555, from ExoView kit) and HIV-1 gp120 (gp120-AF488, from Novus Biologicals, #NBP3-06630AF488), and 2 µL of smFISH probes (from 100 ng/µL smFISH probe stock) ...
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bioRxiv - Genomics 2024Quote: ... We performed flow cytometry (MACSquant VYB) to confirm that >90% of the PGP1 hiPSCs are positive for TRA-1-60 (Novus Biologicals NB100-730F488). hiPSCs were plated using StemFlex media (Thermo Scientific A3349401 ...
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bioRxiv - Immunology 2024Quote: ... Detection of bound bat IgGs was done by incubation for 1 hour at room temperature with mouse anti-bat immunoglobulin (L-chain specific, clone BT1-4F10, Novus Biologicals, CO, USA) diluted to 1 μg/ml in T-PBS followed by incubation for 1 hour with goat-anti mouse IgG conjugated with HRP (Southern Biotech ...
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bioRxiv - Immunology 2024Quote: ... Detection of bound bat F(ab’)2 fragments was done by incubation for 1 hour at room temperature with mouse anti-bat immunoglobulin (L-chain specific, clone BT1-4F10, Novus Biologicals, CO, USA) diluted to 1 μg/ml in T-PBS followed by incubation for 1 hour with goat-anti mouse IgG conjugated with HRP (Southern Biotech ...
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bioRxiv - Cell Biology 2024Quote: ... then washed with PBS and incubated for 1 h with a secondary donkey-anti rabbit antibodies conjugated to DyLight 488nm (Novus Biologicals, Toronto, ON). Cells were then washed three times with PBS and mounted using DAKO fluorescence mounting media (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with a 1:500 dilution of the appropriate primary antibody for 1 h at room temperature (anti-CTCF, Active Motif 61331; anti-SRRM2, Thermo Fisher PA5-59559; anti-SC35, Novus Biologicals NB100-1774 ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed with TBS-T for 10 min before incubating for 1 h at room temperature with a secondary antibody conjugated to goat anti-mouse HRP (NB7539; Novus Biologicals, Centennial, CO, USA) with a titre of 1:10,000 in BLOTTO ...
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Androgen Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in MenbioRxiv - Cell Biology 2020Quote: ... Non-specific antigen binding was blocked by treating the cells with PBS+0.5% BSA prior to adding the primary antibodies (ACE2 or TMPRSS2, Proteintech cat#21115-1-AP and Novus Biologicals cat# NBP1-20984, respectively). Cells were incubated with the primary antibody solution overnight at 4 °C then washed with PBSx3 ...
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bioRxiv - Biochemistry 2024Quote: ... blood plasma was collected at 9 am and ELISA kits were used following manufacturer’s instructions (Leptin: Crystal Chem, 90030; GLP-1: Sigma, EZGLP1T-36K; GDF-15: R&D Systems, MGD150; Adiponectin: Crystal Chem, 80569; FABP4: Novus biologicals, NBP2-82410 ...
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bioRxiv - Cell Biology 2024Quote: ... 60 µm thick vibratome sections were then immunostained with antibodies against Choline Acetyl-Transferase (ChAT) (anti-ChAT, Novus Biologicals, goat, NBP1-30052, 1:10) and HA (anti-HA ...
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bioRxiv - Immunology 2024Quote: ... The membrane was then probed overnight with mouse anti-PNMT primary antibody (1:1000 dilution in 2% w/V BSA in TBS-T; cat. no. NBP2-00688, Novus Biologicals, Centennial, CO, USA), or rabbit glucocorticoid receptor beta (GR-β)-A antibody (1:1000 dilution in 5% w/v BSA in TBS-T ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were incubated in 5% powdered milk + PBS-T with primary antibodies [rat anti-OLLAS (1:1000, L2 clone, Novus Biologicals, Centennial, CO, #NBP1-96713), mouse anti-V5 (1:3000 ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... the native human Piezo1 channel was probed using a mouse monoclonal anti-Piezo1 antibody (Cat# NBP2-75617, Novus Biologicals, Centennial, CO, USA; 1:1,000 dilution); mouse anti-α-actinin antibody (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2020Quote: ... AFL was labeled at the Fc portion of human IgG immunoglobulin (goat polyclonal anti-human IgG Fc antibody, NB7446, Novus Biologicals, 1:200 at 4C overnight). Stainings were followed by a corresponding Alexa Fluor-conjugated secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Primary antibodies and the optimal dilutions and concentrations used are as follows: goat polyclonal anti-spike S2 (Novus Biologicals, AF10774-SP, 1:20, 10 µg/mL), mouse monoclonal anti-dsRNA (SCICONS ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were extensively washed up to eight times in blocking solution and the following antibodies were applied in blocking buffer for immunolabeling: Chicken polyclonal MAP2 (Novus Biologicals NB300-213, diluted 1:1000), Rabbit polyclonal Munc13-1 (Synaptic Systems AB_126103 ...