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Citations for Novus Biologicals :
951 - 995 of 995 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... A second set of ovaries was imaged using N-terminal eIF4ENIF1 rabbit antibody for comparison (2 μg/ml, NBP1-89389, Novus Biologicals, Littleton, CO). Slides were incubated in anti-mouse Alexa Fluor 488 and anti-rabbit Alexa Fluor 594 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Chips were then incubated with fluorescent antibodies against CD81 (CD81-CF555, from ExoView kit) and HIV-1 gp120 (gp120-AF488, from Novus Biologicals, #NBP3-06630AF488), and 2 µL of smFISH probes (from 100 ng/µL smFISH probe stock) ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2% Triton X-100 and 1% bovine serum albumin for 1 hour and then stained overnight at 4°C with primary antibodies sheep anti- TH (1:1000, Novus biologicals, NB300-109), rabbit anti-Vamp2 (1:1000 ...
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bioRxiv - Biophysics 2022Quote: ... Immunofluorescence images of DMSO and dTAG-13 treated WT Hela cells and TCOF1 degron cells stained using TCOF1 antibody (Novus Biologicals, NBP1-86909) and HA antibody (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... Sections were then incubated with primary antibodies (anti-NeuN: 1:50, MAB377X, Millipore, Billerica, Massachusetts, United States; anti-RBPMS:1:1000, Novus Biologicals NBP2-20112) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... then overnight with primary antibodies (1:500 RBPMS, PhosphoSolutions #1832-RBPMS; 1:400 CHX10, Novus Biologicals #NBP1-84476; 1:50 AP2A, DSHB #3B5) at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... All transcripts were purified by silica-column chromatography followed by cellulose purification to remove dsRNA impurities.44 The presence of dsRNA impurities was determined by dot blot (500 ng/dot) using J2 dsRNA-specific antibody (Novus Biologicals, NBP3-11395). The length and integrity of mRNA were validated on an Agilent TapeStation system (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... 250ug of lysate was precleared with 20 μl of packed Protein A Sepharose CL-4B resin (Cytivia, 17078001) for 2 hours at 4 °C and then incubated overnight with 3ug of anti-NPM1 antibody (Novus Biologicals, NB600-1030) at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The antibody against K11-linked ubiquitin chains (Cat. No. NBP3-05681, kindly provided by A. Buchberger,) was purchased from Novus Biologicals (Littleton, USA). The antibody against Sam50 was custom- generated against the full-length protein (Gramsch laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... then washed with PBS and incubated for 1 h with a secondary donkey-anti rabbit antibodies conjugated to DyLight 488nm (Novus Biologicals, Toronto, ON). Cells were then washed three times with PBS and mounted using DAKO fluorescence mounting media (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with a 1:500 dilution of the appropriate primary antibody for 1 h at room temperature (anti-CTCF, Active Motif 61331; anti-SRRM2, Thermo Fisher PA5-59559; anti-SC35, Novus Biologicals NB100-1774 ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed with TBS-T for 10 min before incubating for 1 h at room temperature with a secondary antibody conjugated to goat anti-mouse HRP (NB7539; Novus Biologicals, Centennial, CO, USA) with a titre of 1:10,000 in BLOTTO ...
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bioRxiv - Immunology 2020Quote: ... The EVs pellet suspended in RIPA was used to perform western blot to check the expression of ESAT-6 (anti-ESAT-6 antibody (#ab26246, AbcamInc, USA) and CD63 (#NBP2-42225SS, Novus Biologicals, Centennial, CO, USA).
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Androgen Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in MenbioRxiv - Cell Biology 2020Quote: ... Non-specific antigen binding was blocked by treating the cells with PBS+0.5% BSA prior to adding the primary antibodies (ACE2 or TMPRSS2, Proteintech cat#21115-1-AP and Novus Biologicals cat# NBP1-20984, respectively). Cells were incubated with the primary antibody solution overnight at 4 °C then washed with PBSx3 ...
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bioRxiv - Cell Biology 2024Quote: ... 60 µm thick vibratome sections were then immunostained with antibodies against Choline Acetyl-Transferase (ChAT) (anti-ChAT, Novus Biologicals, goat, NBP1-30052, 1:10) and HA (anti-HA ...
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bioRxiv - Biophysics 2022Quote: ... Coverslips were then incubated overnight at 4°C in a 1:100 dilution of primary antibody (anti-UBTF, Novus Biologicals, NBP1-82545; anti-POLR1A, Novus Biologicals, NBP2-56122; anti-Fibrillarin, EMD Millipore, MABE1154; anti-MPP10, Novus Biologicals, NBP1-84341 ...
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bioRxiv - Molecular Biology 2024Quote: ... IdU/CldU fibers were stained using standard immunostaining with antibodies against IdU (anti-BrdU, clone B44, Beckton Dickinson #347580, 1:50) and CldU (anti-BrdU BU1/75, Novus Biological ab6326, 1:100) followed by sequential labelling with secondary antibodies (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... The membrane was then probed overnight with mouse anti-PNMT primary antibody (1:1000 dilution in 2% w/V BSA in TBS-T; cat. no. NBP2-00688, Novus Biologicals, Centennial, CO, USA), or rabbit glucocorticoid receptor beta (GR-β)-A antibody (1:1000 dilution in 5% w/v BSA in TBS-T ...
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bioRxiv - Immunology 2024Quote: ... and then stained with anti-Rb antibodies and their corresponding secondary IgG (#MA5-11387 Thermo Fisher Scientific, Waltham, MA, #NB100-56598 Novus Biologicals, Centennial, CO).
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bioRxiv - Neuroscience 2024Quote: ... Sections were subsequently incubated for 48 hours at 4°C with primary antibodies: rabbit-anti neurokinin B (1:1000, Novus Biologicals, catalog #NB300-201), guinea pig anti-cFos (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... For experimental IHC and mIHC we stained the following antibodies for 1 hour at room temperature at the described concentrations: ADPH (1:1000, Novus Biologicals Cat: NB110-40878), CD45 (1:50 ...
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bioRxiv - Developmental Biology 2021Quote: ... permeabilized gonads were subsequently incubated with primary antibodies overnight at 4°C [rat anti-OLLAS (1:1000, L2 clone, Novus Biologicals, Centennial, CO, #NBP1-96713), rabbit anti-V5 (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were incubated in 5% powdered milk + PBS-T with primary antibodies [rat anti-OLLAS (1:1000, L2 clone, Novus Biologicals, Centennial, CO, #NBP1-96713), mouse anti-V5 (1:3000 ...
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bioRxiv - Neuroscience 2020Quote: ... before incubation with primary antibodies (anti-NLGN3 C-terminus, Synaptic Systems, 1:1000, RRID: AB_2619816.; anti-NLGN3 N-terminus, Novus Biologicals, 1:1000, RRID: AB_11027178) in blocking buffer with 0.01% sodium azide for 10 minutes at room temperature ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... the native human Piezo1 channel was probed using a mouse monoclonal anti-Piezo1 antibody (Cat# NBP2-75617, Novus Biologicals, Centennial, CO, USA; 1:1,000 dilution); mouse anti-α-actinin antibody (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... 5-10 μg of total proteins were loaded for each sample on the Invitrogen 4-12% Bis-Tris gel and the following antibodies were used for immunoblot detection: rabbit anti-Synj1 (Novus Biologicals, NBP1-87842, 1:1000), rabbit anti-GFAP (Abclonal ...
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bioRxiv - Genetics 2022Quote: ... Membranes were blocked with 5% milk in 1xTBS-Tween (10mM Tris, 150 mM NaCL, 0.1% Tween20) for 60 minutes then blotted with primary antibodies (Set8: Novus Biologicals 44710002; β-tubulin: Abcam ab6046) in 5% milk in 1xTBS-Tween overnight ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 hour at room temperature and then incubated overnight at 4°C in a humid chamber with primary antibodies (SARS-CoV-2-N, GeneTex GTX635679, at 1:100; CD163,Novus Biologicals NBP2-48846, 1:250) diluted in 1% BSA/0.1% Triton-X PBS (PBST) ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked for 1 hour and incubated overnight at 4°C with the NPC1 primary antibody (rabbit polyclonal, Novus Biologicals, UK, NB 400-148).
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bioRxiv - Neuroscience 2024Quote: ... Tissue was incubated overnight at 4°C with primary antibody directed to ionized calcium-binding adaptor molecule 1 (IBA1) (Novus Biologicals NB100-1028, 1:500) diluted in CytoQ containing 0.05% Tween-20 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections (50 µm thickness) were cut via freezing microtome and stained with anti-c-fos primary antibody (Cell Signaling Technology 2250T or Novus Biologicals NBP2-50037SS), and nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were blocked with nonfat milk at room temperature for an hour and incubated overnight at 4°C with a primary antibody: sulfatase 1 (1:200 dilution; Novus Biologicals, Cat# NBP231584, RRID: AB_2916043) and sulfatase 2 (1:500 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were blocked with 5% non-fat milk at room temperature for an hour and incubated overnight at 4°C with a primary antibody: heparanase (1:200 dilution; Novus Biologicals, Cat# NBP303846, RRID: AB_2927437) diluted in 5% non-fat milk ...
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bioRxiv - Physiology 2023Quote: ... Incubation with primary antibody was followed by 3 washes and membranes were incubated 2 hours with goat anti-Human IgG (Novus biological, NBP1-75006, 1/10000), goat anti-rabbit IgG (Cell signaling ...
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bioRxiv - Cell Biology 2024Quote: ... for 15 min and blocked with 4% BSA in PBS for 1 h and overnight incubation of α-SMA antibody (Novus biologicals, Cat#NB300-978) diluted (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue sections were probed with the following antibodies: SLC7A11 (Cell Signalling Technologies, Cat. 12691, 1:25; Abcam, Cat. ab37185, 1:2000; Novus Biologicals, Cat. NB300-318, 1:2000), biotinylated anti-rabbit secondary antibody (Vector Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... cells are incubated with the primary antibody anti-myosin heavy chain (MF20, mouse monoclonal, DHSB, diluted 1:2) or anti-ki67 (rabbit polyclonal, Novus Biologicals #NB110-89717, diluted 1:200) for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were then incubated overnight at 4°C in a 1:100 dilution of primary antibody (anti-MPP10, Novus Biologicals, NBP1-84341; anti-Fibrillarin, EMD Millipore, MABE1154; anti-POLR1A, Novus Biologicals, NBP2-56122; anti-UBTF, Novus Biologicals, NBP1-82545) in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were incubated in 5% normal donkey serum in PBST overnight at 4°C: IBA1 (Novus Biologicals #NB100-1028 in goat, 1:1000); 6E10 (BioLegend #803001 in mouse ...
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bioRxiv - Neuroscience 2020Quote: ... AFL was labeled at the Fc portion of human IgG immunoglobulin (goat polyclonal anti-human IgG Fc antibody, NB7446, Novus Biologicals, 1:200 at 4C overnight). Stainings were followed by a corresponding Alexa Fluor-conjugated secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... a consecutive heart section was treated similarly except that the primary MC1-R antibody was replaced by purified normal rabbit IgG (Novus Biologicals, Littleton, CO, USA, # NB810-56910). Sections were counterstained with hematoxylin (CarlRoth) ...
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bioRxiv - Microbiology 2024Quote: Primary antibodies and the optimal dilutions and concentrations used are as follows: goat polyclonal anti-spike S2 (Novus Biologicals, AF10774-SP, 1:20, 10 µg/mL), mouse monoclonal anti-dsRNA (SCICONS ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were extensively washed up to eight times in blocking solution and the following antibodies were applied in blocking buffer for immunolabeling: Chicken polyclonal MAP2 (Novus Biologicals NB300-213, diluted 1:1000), Rabbit polyclonal Munc13-1 (Synaptic Systems AB_126103 ...
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bioRxiv - Physiology 2024Quote: ... chromatin extracts were prepared from FXR floxed and FXR-LKO mouse livers after treatment of the mice with GW4064 which was followed by preclearing and immunoprecipitation using control IgG or FXR antibody (Novus Biologicals, NBP2-16550; Santa Cruz, sc-25309), RXRα antibody (Proteintech ...
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bioRxiv - Biophysics 2023Quote: ... The blot was blocked in 5% BSA in TBST and subsequently incubated with primary antibody (anti-mDia1, #20624-1-AP, Thermo Fischer Scientific, 1:1000, overnight at 4° C; GAPDH, #NB300-221, Novus Biologicals, 1:40.000, 1-2 hrs at RT). Secondary antibody staining was performed for 1 hour at RT in blocking solution (Donkey-Anti-Rabbit-HRP ...