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Citations for Novus Biologicals :
851 - 900 of 995 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were diluted in permeabilization buffer and incubated for 1 h at room temperature: mouse anti-PDI (clone RL90, Novus Biologicals), 10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... Negative controls without the primary antibodies or using IgG isotypes (Goat IgG NB410-28088, and Rabbit IgG NBP2-24891, Novus Biologicals) at the same dilution as the primary antibodies were used.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then incubated for 1 hour at 37°C with γ-H2AX primary antibody (NB100-384, Novus Biologicals, Centennial, CO) at 1:1000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... 300-500 µg of protein were diluted in 250 µl IP buffer and incubated for 3h in head-to-toe agitation at 4°C with 0.5 µg of the specific antibody: rabbit polyclonal rabbit anti-CLUH [#NB100-93305 (1) and #NB100-93306 (2) from Novus Biologicals] ...
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bioRxiv - Cancer Biology 2020Quote: ... Mycoplasma testing was performed regularly to confirm absence of mycoplasma contamination on cells with SR-B1 rabbit polyclonal antibody (NB400-104) was purchased from Novus Biologicals. GAPDH rabbit polyclonal antibody (sc-25778 ...
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bioRxiv - Cell Biology 2021Quote: ... Incubation with purified Miro1 Nbs or AF647 conjugated Nbs (100 – 200 nM in 5% BSA in TBST) or rabbit anti-Miro1 antibody (# NBP1-89011, Novus Biologicals) was performed overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... then incubated in primary conjugate lectin from Wisteria floribunda agglutinin (WFA; 1:250, L1516, MilliporeSigma) and primary rabbit anti-PV antibody (1:1000, NB-120-11427, Novus Biologicals) at 4°C for 48 hours in PBS containing 0.3% Triton™ X-100 (PBST ...
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bioRxiv - Systems Biology 2020Quote: ... performing all incubations and washes with 1% BSA-PBS and staining with 1:50 primary antibody (goat anti-human AXL AF154 from Novus Biologicals) and 1:60 secondary antibody (bovine anti-goat conjugated to Alexa Fluor 647 ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... biotinylated and flow-through samples were loaded for Western blotting then probed with mouse monoclonal anti-Piezo1 antibody (Cat. No. NBP2-75617, Novus Biologicals) and mouse anti-α-actinin antibody (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked in 5% BSA and then probed with a mouse monoclonal antibody to mCherry (Novus Biologicals, Littleton CO) at 1:1000 at 4°C overnight ...
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bioRxiv - Biophysics 2021Quote: ... The transduced MEFs were then selected with 400µg/ml G418 for 7 days and subsequently screened for LA or LC KD by immunofluorescence microscopy and immunoblotting with antibodies specific for either LA (rabbit #321) or LC (EM-11 Novus Biologicals).
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bioRxiv - Pathology 2022Quote: ... Megalin and KIM-1 were detected by IF using primary antibodies against megalin (anti-MC220)60 and TIM-1/KIM-1/HAVCR (#AF1817, Novus Biologicals)61 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 time for 5 mins and 3 times for 2 hours in PBS supplemented with 0.3% Triton X-100 (PBS-T) and then incubated in primary antibody solution in PBS-T (goat anti-fluorescein (Novus Biologicals NB600-493 ...
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bioRxiv - Immunology 2021Quote: All cell culture reagents were purchased from Life Technologies (USA), unless otherwise noted. Antibodies: PE-conjugated anti-mouse iNOS (Cat. #NBP2-22119, Clone 4E5) was purchased from Novus Biologicals (USA). FITC-conjugated anti-mouse CD68 (Cat ...
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bioRxiv - Microbiology 2020Quote: ... and stained for immunohistochemistry for SARS-CoV-2 nucleocapsid using a monoclonal anti-SARS-CoV-1 nucleocapsid antibody (NB100-56576, Novus Biologicals) on deparaffinized sections on the Ventana Discovery Ultra platform (Roche) ...
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bioRxiv - Immunology 2020Quote: ... from HEK293 cells transfected with HIF-encoding vectors or from Thy-1.1-purified CD8+ T cells transduced with HIF-encoding vectors were probed with polyclonal antibodies against HIF-1α (NB-100-449 or NB-100-105 Novus Biologicals), HIF-2α (AF2997 ...
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bioRxiv - Cell Biology 2021Quote: ... Reciprocal co-IPs were performed on whole rat retina lysate to confirm identified Tulp1-binding partners using the following target antibodies: MAP1B (NB100-68256, Novus Biologicals), Kif3a (EPR5087 ...
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bioRxiv - Cell Biology 2020Quote: For Western blot detection the following antibodies were used: anti-Rab13 rabbit polyclonal (Novus Biologicals, cat# NBP1-85799, 1/1,000 dilution), anti-GFP rabbit polyclonal (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: Liver metastatic tissue was sliced at 10 μm thickness with a microtome and stained overnight at 4 °C with an anti-Ki67 antibody (Novus Biologicals). The mean numbers of positive cells at 400⍰ magnification were compared between the two groups ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Membrane was stripped using 0.1 N NaOH and reprobed with rabbit anti-beta actin antibody (1:10,000, Novus Biologicals, CO, USA) and detected as above.
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bioRxiv - Immunology 2020Quote: ... Samples were blocked with 3% FBS in 1X-PBS followed by incubation with anti-CitH3 antibody (1:100, NB100-571350, Novus Biologicals) and Alexa fluor 488-conjugated anti-rabbit antibody (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... The slides were incubated for 1 hour with anti-F4/80 primary antibody (1:900 dilution; NB600-404 Novus Biologicals LLC), followed by 15minute secondary antibody incubation (1:7500 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were resuspended with antibody buffer (wash buffer with 0.005% digitonin and 2 mM EDTA) and incubated with 2μg of YAP-antibody (Novus Biologicals, NB110-58358) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were then incubated overnight at 4°C with the following primary antibodies diluted in blocking solution: rabbit anti-cleaved caspase 3 (1:200, Novus Biologicals), rat anti-CTIP2 (1:500 ...
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bioRxiv - Pathology 2022Quote: ... Membrane was blocked with 5% non-fat milk in TBST for 2 hrs and probed overnight at 4°C with the primary antibody against TIM-1/KIM-1/HAVCR (#AF1817, Novus Biologicals). After washing the membrane with TBST ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were analysed by gel electrophoresis on a 12 % SDS-PAGE gel and immunoblotting using anti-PINK1 rabbit antibody (1:500; Novus Biologicals), anti-β-actin mouse (AC-74 ...
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bioRxiv - Neuroscience 2022Quote: ... The sciatic nerves were fixed and immune-stained with anti-SCG10 antibody (NBP1–49461, 1:500; Novus Biologicals, Littleton, CO, USA). Regenerated axons were measured and quantified using ImageJ software.
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bioRxiv - Neuroscience 2022Quote: ... Samples were blocked with 1% BSA in PBS for 20 min at RT and subsequently incubated overnight at 4°C with primary antibodies: rRNA (mouse, 1:250, Novus Biologicals) and MAP2 (chicken ...
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bioRxiv - Neuroscience 2022Quote: ... slices from C57Bl/6j mice were prepared as follows: brain sections were incubated at 4°C with the following primary antibodies: MBP (rabbit, 1:300, Novus Biologicals), Sox9 (goat ...
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bioRxiv - Neuroscience 2022Quote: ... were stained for GAP43 as a marker of immature OSNs (anti-GAP43 primary antibody, 1:1000 for 48 h at 4 °C, NB300-143, Novus Biologicals; donkey anti-rabbit-Alexa Fluor 546 secondary antibody ...
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bioRxiv - Molecular Biology 2023Quote: LOX and TG2 protein expressions in the lung and heart tissues were detected using immunohistochemistry (IHC) assays with antibodies against LOX (1:250; NB100-2527, Novus Biologicals) and TG2 (1:250 ...
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bioRxiv - Molecular Biology 2024Quote: ... The sections were stained with Hematoxylin & Eosin (H&E) staining performed by routine methods and with primary antibodies directed at CD31 (NB100-2284, Novus Biologicals) and ACKR1 (566424 ...
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bioRxiv - Immunology 2023Quote: ... were fluorescently labelled using Lightning-Link® Rapid Alexa Fluor 488 Antibody Labelling Kits in accordance with the manufacturer’s instructions (Novus Biologicals). Macrophages were incubated with the labelled proteins or anti-mouse F4/80 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: Paraffin embedded lung tissues were cut with a Leica RM2235 Microtome and stained for rabbit polyclonal STING/TMEM173 antibody (Novus Biologicals #NBP2-24583SS ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibody incubations were kept overnight with anti-TOM20 (1:75, WH0009804M1) and anti-LC3 (1:200, Novus Biologicals, NB100-2220). Secondary antibody incubation was for 3 h with Alexa Fluor 647 goat anti-mouse IgG (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were blocked in 3% BSA + 0.5% Triton-X in 1XPBS for 30 minutes and incubated with the following primary antibodies diluted in the same buffer for 90 minutes at RT: anti-FANCD2 (NB 100-182, Novus Biologicals) 1:200 ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked using 5% milk in TBST buffer for 1 h in orbital shaking and incubated overnight with 1:10,000 dilution of Anti-His mouse monoclonal antibodies (Novus Biologicals, AD1.1.10). The SDS-PAGE and Western Blot Analysis results were imaged using Azure® Biosystems Imager.
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were placed on 50 µL drops of the primary antibody mixture consisting of rat anti-alpha tubulin at 1:100 dilution (Novus biological); mouse anti-lamin A/C at 1:100 dilution (Cell Signaling Technology) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained sequentially with a rabbit polyclonal Krt19 antibody conjugated to Alexa Fluor 647 (1:200; catalog number NB100-687AF647; Novus Biologicals) and a rat monoclonal Cd9 antibody conjugated to Alexa Fluor 750 (1:200 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed once with PBS and incubated with a mouse pan-flavivirus anti-Env antibody (Mab 4G2, Novus Biologicals NBP2) for 1-2 h at room temperature (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed with 1x TBST 3 times and incubated for 5 minutes 3 times between antibody incubations and before addition of NovaLume Atto Chemiluminescent Substrate AP (Novus Biologicals). Membranes were then imaged on a ChemiDoc SRS Imaging System (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... virus titers were determined by focus forming assay using flavivirus group antigen antibody anti-E (clone 4G2, diluted 1:1000, 4G2, Novus Biologicals).
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bioRxiv - Molecular Biology 2023Quote: ... Pellets were subsequently incubated for one hour at room temperature with flavivirus group antigen antibody (clone 4G2, diluted 1:100, Novus Biologicals) for flavivirus-infected cells and murine-produced Clone 9E10 primary antibody specific for NS5A (kindly provided by Charles Rice at The Rockefeller University ...
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bioRxiv - Cell Biology 2023Quote: For GGA1 and NHE6 co-localization with respect to Golgi/Endosomes in 5x expanded cells in hydrogels were labelled with a cocktail of primary antibodies (1:500) of mouse GGA1 (H00026088-M01, Novus Biologicals), rabbit NHE6 (Covance) ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were blocked and then incubated with morphology marker antibodies to guide region of interest (ROI) selection: PanCK (488 channel, NBP2-33200AF488, Novus Biologicals), CD68 (594 channel ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked in PBS containing 0.2% Triton-X and 5% bovine serum albumin and stained for GFP or YFP using an anti-GFP antibody (NB100-1614, 1:200, Novus Biologicals, Biotechne ...
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bioRxiv - Cell Biology 2023Quote: ... Bradford assay was used to quantify the protein concentration and 400 μg of cell lysate was diluted in 250 μl of IP buffer and incubated with rabbit polyclonal anti-CLUH antibody (NB100-93306, Novus Biologicals) or rabbit IgG isotope control antibody (NB810-56910 ...
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bioRxiv - Molecular Biology 2023Quote: ... sectioned samples (human or murine) were incubated overnight with primary antibodies: anti-RPE65 (1:400, Cat# NB100-355, Novus Biologicals, USA), anti-ZEB2 (1:400 ...
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bioRxiv - Bioengineering 2024Quote: ... followed by the incubation of primary antibodies (collagen V: AB7046, 1:200 dilution, Abcam; tenascin-C: AB108930, 1:200, Abcam; LOX: NBP2-24877, 1:100, Novus Biologicals) at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... followed by overnight incubations with various pairs of primary antibodies directed either against FLVCR1 (Novus Biologicals, #NB100-1481, dilution 1:100), TANGO2 (Invitrogen ...