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Citations for Mirus Bio :
301 - 338 of 338 citations for 6H Benzo g 1 4 diazonino 7 6 5 cd indol 6 one 10 ethenyl 1 3 4 5 7 8 10 11 12 13 decahydro 4 hydroxymethyl 8 10 13 trimethyl 7 13 bis 1 methylethyl 4S 4R* 7R* 10S* 13S* 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The following day 3 µl of Mirus TransIT 2020 reagent (Mirus Bio, Madison, WI) and 1 µg of plasmid were combined in 250 µl Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: ... in combination with 3μg psPAX2 (packaging plasmid) and 2μg pMD2.G (envelope plasmid) with 30μL TransIT-LT1 transfection reagent (Mirus, MIR 2300). For T-ALL cell infection ...
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bioRxiv - Cell Biology 2023Quote: ... and transfected with 1.5 µg of the indicated plasmid DNA and 0.5 µg of the PiggyBac transposase construct using TransIT-X2 (MIR6004, Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Monoclonal and polyclonal hIPSC-dCas9 lines were transfected with pCS2-iREP-GFP-PGK-BFP-U6-gRNA-irep or pCS2-iREP-GFP-PGK-BFP-U6-gRNA-mock vectors as replicates in 12 well plates using TransIT®-LT1 Transfection Reagent (Mirus Bio, MIR2300) according to manufacturer’s instructions and as detailed above ...
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bioRxiv - Synthetic Biology 2021Quote: ... with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using Mirus Trans-IT Lenti (Mirus #MIR6606). Primary T cells were thawed the same day ...
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Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using Mirus Trans-IT Lenti (Mirus #MIR6606). Primary T cells were thawed the same day ...
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bioRxiv - Immunology 2022Quote: ... were transfected with pHR SIN including cloned transgene and packaging vectors pMD2.G and pCMVdR8.91 using TransIT-Lenti Transfection Reagent (Mirus #MIR6603). On the day of transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... and pMD2.G (VSVG envelope vector) vectors were transfected into HEK293T cells in 94 mm2 dish using TransIT-LT1 Transfection Reagent (Mirus). The supernatants containing virus particles was collected at 72 hours after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... and 20.5 µg pcDNA3.1 VSV-G in 4.9 ml DMEM with 345 µl TransIT-Lenti Transfection Reagent (Mirus Bio, MIR 6600) added to 4.8 ml DMEM ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells were transfected with retroviral packaging plasmids (pVSV-G, pBS-CMV-gagpol) and pMRX-IP-HaloTag7-LC3 using TransIT-LT1 (Mirus). The viral supernatants were collected 72 hr after transfection followed by virus concentration using Lenti-X concentrator (TaKaRa) ...
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bioRxiv - Genomics 2023Quote: ... 4ug of packing plasmids psPAX.2 and 2ug of the envelope vector pMD2.G diluted in OptiMEM medium and Trans-Ltl transfection reagent (Mirus). For the generation of gRNA lentivirus ...
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bioRxiv - Molecular Biology 2024Quote: ... constructs with inserted genes were co-transfected with vectors expressing lentiviral gag/pol (psPAX2) and vsvg (pMD2.G) genes in HEK293T cells to produce lentiviruses by TransIT-LT1 transfection reagent (Mirus). Supernatants containing virus particles were collected at 48 hours and 72 hours following transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... or Gqi5 reporter plasmid (3 μg/plate) was carried out with TransIT-X2® System (Mirus). The cells were serum-starved for 16 h ...
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bioRxiv - Microbiology 2019Quote: ... Lentivirus was generated by co-transfecting pCDH-G3BP1-T2A-puro-MSCV with pCMV-VSV-G and pSPAX2 into 293T cells with Trans-IT LT1 (Mirus Biosciences) per manufacture instructions ...
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bioRxiv - Genomics 2022Quote: Lentivirus was generated by transfecting HEK239T cells with the transfer plasmid and four packaging plasmids (for expression of VSV-G, Gag/Pol, Rev, and Tat) using TransIT-LT1 Transfection Reagent (Mirus Bio). Viral supernatant was harvested two days after transfection and filtered through 0.44 µm PES filters and/or frozen at –80 °C prior to transduction.
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bioRxiv - Microbiology 2023Quote: ... were cotransfected with 1 μg psPAX2-IN/HiBiT,23 1 μg pWPI-Luc2,23 and 500 ng plasmids expressing parental S or its derivatives using TransIT-293 transfection reagent (Mirus, Cat# MIR2704) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Lentiviral vector was co-transfected with pSPAX2 and pMD2.G plasmids (gifts from Dr. Julius lab) to HEK293T by TransIT-Lenti transfection reagent (Mirus Bio). The lentivirus was produced in DMEM containing 10% FBS and 1% BSA and then concentrated by the Lenti-X concentrator (Takara Bio).
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bioRxiv - Molecular Biology 2023Quote: ... pCW57.1-HLTF vectors were packaged into lentivirus particles using the 2nd generation lentiviral packaging system (pMD2.G & psPAX2) in HEK293T cells using TransIT®-LT1 Transfection Reagent (Mirus). Virus-containing media was harvested 24 & 48h post-transfection and filtered through 0.45 μm PES membrane syringe filter to eliminate packaging cells ...
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bioRxiv - Cell Biology 2019Quote: ... or a GFP expression vector with packaging vectors (CMVΔR8.91, CMV-VSV-G) into 293T cells using Mirus TransLTi (Mirus Bio LLC, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... shUBR5 and shScrambled-pLKO.1-puro were co-transfected with pCMV-VSV-G and pCMV-dR8.2 dvpr plasmids into HEK 293T cells using TransIT293 reagent (Mirus Bio LLC, USA). To generate stable silenced shUBR5 cells ...
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bioRxiv - Cell Biology 2021Quote: Lentivirus is produced in HEK293t cells by transfection of lentiviral plasmid and packaging plasmids pMD2.G and psPAX2 using TransIT-VirusGEN® Transfection Reagent (Mirus, MIR6705). After two days of transfection ...
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bioRxiv - Cell Biology 2023Quote: ... the packaging cell line HEK293-FT was transfected with pTCP-MPsfGFP together with pSAX2 (12260 Adgene) and pCMV-VSV-G (8454 Adgene) plasmids using the kit TransIT®-293 Transfection Reagent (Mirus Bio) and following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cell-free viruses were produced by transfection of HEK293 cells with pNL4-3 using TransIT-LT1 (Mirus) or Fugene HD (Roche ...
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bioRxiv - Bioengineering 2022Quote: Transfection of human pancreatic MIA PaCa-2 and BxPC-3 cells was performed with TransIT-mRNA (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... MCF10A cells were treated with an additional 750 μL of medium containing 3 μM puromycin (Mirus Bio 5940), achieving a final concentration of 1 μM (0.5 μg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... the transfer plasmid and helper plasmids VSV-G and PSP (generously provided by David Sanders) were transfected into the Lenti-X cells using Transit293 transfection reagent (Mirus, Cat. No. MIR 2700) and incubated in OptiMEM (ThermoFisher Cat No ...
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bioRxiv - Cell Biology 2021Quote: ... or 0.25 µg KDELR-GFP and 0.5 µg pcDNA3.1 (− ligand) diluted in 100µl Optimem and 3 µl Mirus LT1 (Mirus Bio LLC). After a further 18 h ...
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bioRxiv - Microbiology 2021Quote: ... and the VSV-G-envelope-expressing plasmid pMD2G as well as pRDI292 into 293T cells with TransIT-LT1 transfection reagent (Mirus Bio LLC, Madison, WI, USA).
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bioRxiv - Microbiology 2019Quote: ... 100ng of RIG-I agonist derived from the 3’-UTR of hepatitis C virus (55) was transfected per 1e6 cells using TransIT-mRNA transfection kit (Mirus). For stimulation of MDA5 signaling ...
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bioRxiv - Microbiology 2019Quote: ... 100ng of RIG-I agonist derived from the 3’-UTR of hepatitis C virus (19) was transfected per 1e6 cells using TransIT-mRNA transfection kit (Mirus). For stimulation of MDA5 signaling ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transiently transfected with 3 µg pE-hABCA3-eGFP plasmid (hABCA3-eGFP expression controlled by CMV promoter) in Trans-IT LT1 transfection reagent (Mirus). 48 h post-transfection the medium was removed ...
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bioRxiv - Molecular Biology 2023Quote: ... reporter plasmid delivery (3 µg/plate for cFSHr and 0.3 µg/plate for cLHr) was carried out with TransIT-X2® System (Mirus). The cells were serum-starved for 16 h ...
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bioRxiv - Systems Biology 2021Quote: ... torque teno virus hypothetical protein or human respirovirus 3 D protein were transfected into the cells using TransIT-LT1 (Mirus Bio) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Approximately 250,000 293T cells were seeded in 6-well plates and transfected the next day with 100 ng pcDNA5/TO-A3-EGFP and 200 ng pcDNA4/TO-RNR-3×FLAG using TransIT-LT1 (Mirus #2304). Cells were harvested ~24 hours after transfection and resuspended in 500 µl of lysis buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... in 12-well plates and were transfected with 3 μg capped in vitro transcribed DENV replicon RNA using the TransIT-mRNA Transfection Kit (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... BHK-21 cells cultured in 12-well plates were transfected with 3 μg capped in vitro transcribed DENV2 RNAs using the TransIT-mRNA transfection kit (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Huh7 cells in 6- well plates were transfected with 3 μg of each in vitro transcribed RNA using the TransIT mRNA transfection kit (Mirus Bio) as previously described (33) ...
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bioRxiv - Microbiology 2021Quote: ... cells were transiently transfected with 19 μg pNL4-3 or the respective proviral DNA using TransIT®-LT1 transfection reagent (Mirus Bio LLC) according to the manufacturer’s instructions ...