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101 - 110 of 110 citations for Medium Kit without Serum and with CultureBoost R since 2019

• Chikungunya virus glycoproteins transform macrophages into productive viral dissemination vessels

  Zhenlan Yao, et al., bioRxiv - Microbiology 2023

  Quote: ... 500 ng of viral genomic RNA was transfected per well in 12-well plates through the TransIT®-mRNA Transfection Kit (Mirus Bio) following manufacturer’s instructions.
• Detection of tumor-derived extracellular vesicles interactions with immune cells is dependent on EV-labelling methods

  Luisa Loconte, et al., bioRxiv - Cell Biology 2023

  Quote: ... the packaging cell line HEK293-FT was transfected with pTCP-MPsfGFP together with pSAX2 (12260 Adgene) and pCMV-VSV-G (8454 Adgene) plasmids using the kit TransIT®-293 Transfection Reagent (Mirus Bio) and following manufacturer’s instructions ...
• Light-sensitive phosphorylation regulates enzyme activity and filament assembly of human IMPDH1 retinal splice variants

  S. John Calise, et al., bioRxiv - Biochemistry 2023

  Quote: ... culture medium was replaced with 345 µL fresh DMEM per well and mammalian expression plasmids were transfected into cells using the TransIT-HeLaMONSTER Transfection Kit (Mirus Bio #MIR2904) according to the manufacturer’s protocol ...
• Adapting CRISPR/Cas9 System for Targeting Mitochondrial Genome

  Syed-Rehan A. Hussain, et al., bioRxiv - Molecular Biology 2020

  Quote: ... Transient transfection with synthetic mRNA of Cas9 and sgRNAs was performed in either MEF or 293K cells using the TransIT®-mRNA Transfection Kit (Mirus Bio LLC) in OptiMEM medium (Invitrogen) ...
• Viable and efficient electroporation-based genetic manipulation of unstimulated human T cells

  Pinar Aksoy, et al., bioRxiv - Immunology 2019

  Quote: 100 ug of pCMV6 plasmid was labeled with 55 ul of Cy5 Label-IT kit for 1 hour at 37°C (Mirus Bio, Madison, WI). The labeled plasmid was purified by ethanol precipitation ...
• A small molecule RIG-I agonist serves to adjuvant broad multifaceted influenza virus vaccine immunity

  Emily A. Hemann, et al., bioRxiv - Immunology 2022

  Quote: ... or control XRNA (RNA segment derived from the hepatitis C virus genome adjacent to and equivalent in length to polyU/UC that cannot induce RIG-I-dependent innate immune signaling8) using the TransIT®-mRNA Transfection kit (Mirus Bio LLC). Cells were collected in RLT buffer (Qiagen ...
• Respiratory mucosal vaccination of peptide-poloxamine-DNA nanoparticles provides complete protection against lethal SARS-CoV-2 challenge

  Si Sun, et al., bioRxiv - Immunology 2022

  Quote: ... plasmid was fluorescently labeled with the Cy5 fluorophores using the Mirus Label IT® tracker intracellular nucleic acid localization kit (MIR 7021, Mirus Bio, Madison, USA) according to the manufacturer’s instruction ...
• Retrotransposons facilitate the tissue-specific horizontal transfer of circulating tumor DNA between human cells

  Munevver Cinar, et al., bioRxiv - Cancer Biology 2022

  Quote: ... The ctDNA was fluorescently labeled with either Cx-rhodamine or CY5 using the Label IT® Nucleic Acid Labeling kit (Mirus Bio LLC, WI).
• Compact Programmable Control of Protein Secretion in Mammalian Cells

  Alexander E. Vlahos, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... 2.5x105 wildtype K562 cells and 2.5x105 reporter K562 cells were mixed for a total of 5.0x105 cells per well and transfected with various amounts of the purified mRNA using the TransIT®-mRNA Transfection Kit (Mirus Bio, catalog# MIR2250). To induce the reporter cell lines ...
• ENHANCED EFFICIENCY OF RNA-GUIDED CAS12a VERSUS CAS9 TRANSGENE KNOCK-IN AND ACTIVITY AT A SCHISTOSOMA MANSONI GENOME SAFE HARBOR

  Max F. Moescheid, et al., bioRxiv - Molecular Biology 2023

  Quote: The Cas9-sgRNA and Cas12a-crRNA were labeled with CX-rhodamine (Label IT® Nucleic Acid Labeling Reagents Rhodamine Kit, Mirus Bio, Madison, WI) (75 ...