Labshake search
Citations for Mirus Bio :
1 - 50 of 110 citations for Medium Kit without Serum and with CultureBoost R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... px459 CRISPR/Cas9 plasmids with or without gRNA sequence were transfected into HEK293T using TransIT-LT1 transfection reagent (Mirus, MIR 2306). Puromycin (1 μg/mL ...
-
bioRxiv - Biochemistry 2024Quote: ... Two samples each were treated with Opti-MEM with 2 µg of transfection DNA (either pJG01, or negative control without DNA) along with TransIT-X2 transfection reagent (Mirus Bio) premixed and allowed to incubate 5 minutes at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... For the complementation assay 100ng of total DNA (1:1 ratio of plasmids) was transfected into 293T cells in 96-well plates using TansIT(R)-LT1 (Mirus) transfection reagent according to manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2021Quote: ... and 7 μl of VSV-G pseudovirus in 500 μl of 1X opti-MEM reduced serum free media using TransIT-Pro® as a transfection reagent (Mirus). After 4 hr of transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... Strains containing selectable markers were cultured on medium with 50 µg/mL hygromycin (Hygromycin B Solution, Mirus) or 25 µg/mL kanamycin (GoldBio ...
-
bioRxiv - Genetics 2023Quote: ... resuspended in v3.1 medium supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Mirus, MIR 6620), transduced with the hDel-v1 or hDel-v2 lentiviral sgRNA library at a target infection rate of 25% ...
-
bioRxiv - Genetics 2023Quote: ... resuspended in v3.1 medium supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Mirus, MIR 6620), transduced with the hDel-v3 lentiviral sgRNA library at a target infection rate of 10% ...
-
bioRxiv - Cell Biology 2020Quote: ... 1.5 µg plasmid DNA was diluted in 500 µl serum-free RPMI 1640 and supplemented with 12 µl TransIT-LT1 Transfection Reagent (731-0027, Mirus Bio LLC). After 15 min incubation at room temperature ...
-
bioRxiv - Biochemistry 2023Quote: ... MCF10A cells were treated with an additional 750 μL of medium containing 3 μM puromycin (Mirus Bio 5940), achieving a final concentration of 1 μM (0.5 μg/mL ...
-
bioRxiv - Molecular Biology 2022Quote: Preadipocytes were transfected by seeding 30 000 cells per well of a 24 well plate in growth medium and adding 1.5 μl TransIT-X2 (Mirus) and 125 ng plasmid DNA or 1.4 μl LNA (10 μM ...
-
bioRxiv - Molecular Biology 2019Quote: ... cultured in Schneider’s Drosophila Medium containing 10% heat-inactivated FBS and 1X penicillin-streptomycin with the TransIT-LT 1 reagent (Mirus). Flag-CG30403 only (no bait ...
-
bioRxiv - Cell Biology 2020Quote: ... and Ingenio® electroporation kit (#50117, Mirus). After 5 days of transfection ...
-
bioRxiv - Cell Biology 2020Quote: ... and Ingenio® electroporation kit (#50117, Mirus), and seeded onto a 35-mm petri dish with No ...
-
bioRxiv - Neuroscience 2020Quote: ... with the Ingenio electroporation kit (Mirus Bio). The concentration of plasmids we used was 10μg with 100 μl of Ingenio electroporation solution ...
-
bioRxiv - Microbiology 2022Quote: ... using TransIT-mRNA Transfection Kit (Mirus Bio) at 33°C (61) ...
-
bioRxiv - Cell Biology 2022Quote: ... and Ingenio® electroporation kit (Mirus, #50117). After 1 day ...
-
bioRxiv - Microbiology 2023Quote: ... using TransIT-mRNA Transfection Kit (Mirus Bio), or treated with TransIT transfection alone (carrier control) ...
-
bioRxiv - Cancer Biology 2024Quote: ... using a TransIT-X2 transfection kit (Mirus). Tether radius was obtained by comparing tether fluorescence to fluorescence counts from a known area of the parent cell membrane ...
-
bioRxiv - Immunology 2023Quote: All transfections were performed on HEK 293T/17 cells in the presence of serum-free DMEM and Trans-IT transfection reagent (Mirus Bio, Madison, WI, #MIR 2305). All transductions were performed by spinoculation at 1100 x g for 30 min at 30°C.
-
bioRxiv - Cell Biology 2022Quote: ... was added to 100 μL Opti-MEM medium to a final concentration of 900 nM with 7.5 μL transfection reagent TransIT-X2 (Mirus Bio), mixed and incubated at room temperature (RT ...
-
bioRxiv - Genomics 2023Quote: ... 4ug of packing plasmids psPAX.2 and 2ug of the envelope vector pMD2.G diluted in OptiMEM medium and Trans-Ltl transfection reagent (Mirus). For the generation of gRNA lentivirus ...
-
bioRxiv - Bioengineering 2022Quote: ... was labelled with Label It DNA kit (Mirus), following the manufacturer’s instructions.
-
bioRxiv - Immunology 2019Quote: ... or the Ingenio electroporation kit (Mirus, #MIR 50117) with 1.5 µg of either control siRNA (ON-TARGETplus Non-Targeting Pool ...
-
bioRxiv - Immunology 2020Quote: TransIT®-mRNA Transfection Kit (Mirus Bio LLC);
-
bioRxiv - Microbiology 2022Quote: ... using the TransIT-mRNA Transfection Kit (Mirus bio). To stimulate signaling ...
-
bioRxiv - Microbiology 2022Quote: ... using the TransIT-mRNA Transfection Kit (Mirus bio). To induce IFN-β ...
-
bioRxiv - Immunology 2023Quote: ... followed by the MiraCLEAN Endotoxin Removal Kit (Mirus) to ensure efficient removal of endotoxin ...
-
bioRxiv - Immunology 2022Quote: Using the TransIT-mRNA Transfection Kit (Mirus, Madison, WI), 1 × 107 293T cells were transiently transfected with mRNA (20 μg) ...
-
bioRxiv - Cell Biology 2021Quote: Biotin was covalently coupled to pDNA (pGL3 plasmid) by using Nucleic Acid Labeling Kit according to manufacturers’ protocol (Label IT® Nucleic Acid Labeling Kit, Biotin, Mirus Bio, USA). Briefly ...
-
bioRxiv - Cancer Biology 2022Quote: ... were labeled with Cy3 using a Label IT kit (Mirus). B16F10 cells were transfected with labeled sgRNAs and Cas9 protein (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... RNA transfections were performed with TransIT-mRNA Transfection Kit from Mirus Bio ...
-
bioRxiv - Molecular Biology 2022Quote: ... using TransIT-HeLaMONSTER transfection kit according to the manufacturer’s protocol (Mirus). Cells were subsequently incubated in the presence of 50 ng/ml tetracycline to inhibit expression of the target mRNA ...
-
bioRxiv - Immunology 2020Quote: ... and then co-transfected using TransIT-mRNA Transfection Kit (Mirus Bio) with a GFP-expressing mRNA plasmid (Miltenyi Biotec ...
-
bioRxiv - Biophysics 2021Quote: ... RNA was Cy3 labeled using a Label IT kit (Mirus Bio), and RNA purity was verified using a 0.8% agarose gel ...
-
bioRxiv - Immunology 2023Quote: ... mtDNA was biotinylated with Label IT Nucleic Acid Labeling Kits (Mirus) and fragmented with HaeIII (New England Biolabs ...
-
bioRxiv - Microbiology 2023Quote: ... siRNA transfections were performed with TransIT-X2 Transfection Kit (Mirus Bio) following manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells at 50-70% confluency were transfected by overnight incubation with the indicated cDNAs in complete growth medium using TransIT®-LT1 - Transfection Reagent according to the manufacturer’s instructions (Mirus Bio, LLC, cat. # MIR2300; Madison, WI). Stable transfectants were selected in medium containing 500 μg/ml hygromycin B (InvivoGen ...
-
bioRxiv - Immunology 2020Quote: ... In vitro transcribed RNAs were transfected using TransIT-mRNA transfection kit (Mirus). siRNAs directed against CMTR1 (Dharmacon ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were transfected using the TransIT-CHO Transfection Kit (Mirus Bio LLC) (CHO-K1 ...
-
bioRxiv - Immunology 2022Quote: ... RNA PAMP transfections were done using the TransIT-mRNA Transfection kit (Mirus Bio). The siRNA transfections were done using Lipofectamine RNAiMax (Invitrogen) ...
-
bioRxiv - Microbiology 2020Quote: ... PMA-differentiated U937 cells were then transfected with TransIT mRNA transfection kits (Mirus) according to the manufacturer protocol ...
-
bioRxiv - Bioengineering 2023Quote: ... Transfection was performed using the TransIT-PRO® Transfection Reagent & Kit (Mirus Bio). Cells were incubated at 28°C following transfection for 48h ...
-
bioRxiv - Neuroscience 2023Quote: ... XGal staining was performed using the beta-galactosidase staining kit (Mirus #MIR 2600), following the provided protocol ...
-
bioRxiv - Genomics 2019Quote: ... The plasmids were electroporated into K562 cells with Ingenio Electroporation Kit (Mirus, MIR 50115). For each electroporation ...
-
bioRxiv - Immunology 2019Quote: ... RIG-I agonist transfections were done using the TransIT-mRNA Transfection kit (Mirus Bio). The siRNA transfections were done using Lipofectamine RNAiMax (Invitrogen) ...
-
bioRxiv - Plant Biology 2022Quote: ... the purified miRNAs were labeled with Cy5 fluorophore (Cy5 Labeling Kit; Mirus Bio LLC) and then subjected to hybridization with the customized microarray for 4 hours in a 42°C oven ...
-
bioRxiv - Microbiology 2023Quote: ... cells were transfected with Tat mRNA using the Mirus TransIT-mRNA transfection kit (Mirus). Cells that produced mCherry signal after transfection were isolated from the array and isolated as monoclonal populations into 96-well plates for expansion.
-
bioRxiv - Immunology 2021Quote: ... Baculovirus was generated using the flashBac™ kit according to the manufacturer’s instructions (Mirus Bio). The protein was expressed in 2 L of Sf9 cells at 2×106 cells/mL maintained in ESF921 media (Expression Systems ...
-
Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: Transient DNA transfections were carried out using a TransIT-HeLaMONSTER® kit (Mirus Bio LLC), according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Transfection of cells with mRNAs was achieved using a TransIT-mRNA transfection kit (Mirus Bio) according to the manufacturer’s instructions.