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Citations for Megazyme :
351 - 400 of 405 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 5 μL of 40 U/mL Aspergillus niger β-glucosidase (Megazyme Ltd.) was added to 100 μL of filtrate ...
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bioRxiv - Biochemistry 2020Quote: ... according to the reported method.13) Aspergillus niger β-glucosidase was acquired from Megazyme Ltd ...
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bioRxiv - Biochemistry 2020Quote: ... 0.05 units of formate dehydrogenase (Megazyme, Bray, Ireland, Cat. No. E-FDHCB) and 1 mM NAD were added (final reaction volume of 80 μl ...
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bioRxiv - Biochemistry 2020Quote: ... The liberated Glc was quantified using a modified glucose oxidase/peroxidase assay (GOPOD; Megazyme, Wicklow, Ireland) (21) ...
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bioRxiv - Biochemistry 2020Quote: The hydrolysis of α-maltose-1-phosphate was tested using Aspergillus niger amyloglucosidase from Megazyme (high purity for use in Megazyme Total Starch method ...
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bioRxiv - Biochemistry 2020Quote: ... 1,4-β-D-xylopentaose and 1,4-β-D-xylohexaose (Megazyme) were used to identify and quantify the peaks in the chromatograms.
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bioRxiv - Biochemistry 2020Quote: ... debranched arabinan and sugar beet arabinan were from Megazyme (Ireland). Quinoa xylan was prepared according to Gil-Ramirez (Gil-Ramirez et al ...
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bioRxiv - Biochemistry 2020Quote: ... XA3XX and A2,3XX purchased from Megazyme (Irland), were used as substrates for testing arabinofuranosidase activity of GH43_12 ...
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bioRxiv - Biochemistry 2020Quote: ... The following compounds were purchased from Megazyme (Chicago, USA): p-nitrophenyl-α-L-arabinofuranoside (p-NPAra) ...
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bioRxiv - Biochemistry 2020Quote: The hydrolysis of α-maltose-1-phosphate was tested using Aspergillus niger amyloglucosidase from Megazyme (high purity for use in Megazyme Total Starch method) in 100 mM sodium acetate buffer ...
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bioRxiv - Microbiology 2020Quote: ... Turkey) were also used as well as levan from Timothy grass (Megazyme) and in vitro synthesised levan ...
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bioRxiv - Plant Biology 2020Quote: ... using the Megazyme starch assay kit (Megazyme International Ireland), using the AOAC Official Method 996.11 (Approved Methods of the AACC,(McCleary et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... was quantified from culture supernatants using a commercial lactate quantification kit (Megazyme, CAT K-LATE) in 96-well plate format following the manufacturers’ protocol ...
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bioRxiv - Plant Biology 2020Quote: ... RiSLM and Ecp6 were adjusted to 200 μM and 200 μL of each protein was incubated with 200 μL of 2 mM chitohexaose (Megazyme, Wicklow, Ireland), or 200 μL water as control ...
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bioRxiv - Plant Biology 2020Quote: The determination of free Asn content in storage roots was carried out with an L-asparagine kit (Megazyme, K-ASNAM). Measurements were carried out according to(Lecart et al. ...
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bioRxiv - Microbiology 2020Quote: The procedures for β-glucan measurement in the cell wall planktonic cells and ECM of biofilm were performed using enzymatic yeast β-glucan kit purchased from Megazyme International (Ireland) ...
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bioRxiv - Microbiology 2020Quote: ... Citrate in the filtered supernatant was quantified according to manufacturer’s “manual assay” protocol (Megazyme) in ½ reactions ...
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bioRxiv - Microbiology 2020Quote: ... 64-α-D-galactosyl-mannopentaose (Gal2M5) were purchased from Megazyme. Konjac glucomannan digest and carob galactomannan digest were produced in-house using RiGH26 (7 ...
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bioRxiv - Microbiology 2020Quote: ... α-D-galactose-1-phosphate (Gal1P) and α-L-fucose (Fuc) were form Carbosynth and xylotetraose was from Megazyme. Galactose (Gal) ...
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bioRxiv - Biochemistry 2020Quote: ... The enzymes were obtained from Megazyme. They were SCS from an unspecified prokaryotic source (product code E-SCOAS) ...
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bioRxiv - Synthetic Biology 2020Quote: ... or α-glucosidase (Bottle 5 in the Maltose assay kit; Megazyme Inc., Wicklow, Ireland) (Fig ...
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bioRxiv - Plant Biology 2020Quote: ... while 1,1,1-kestopentaose was obtained from Megazyme. Additional inulin and neoseries-type fructans were isolated from onions and barley grain and analysed by mass spectrometry (MS) ...
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bioRxiv - Synthetic Biology 2020Quote: ... cultures were pelleted and sucrose was measured from the supernatant and lysed pellet fractions using sucrose/D-glucose assay kits (Megazyme). Biological replicates were performed in triplicate.
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bioRxiv - Bioengineering 2020Quote: Activity of LsAA9A samples was measured by mixing a reaction solution consisting of 1 mg/mL AZCL-HEC substrate (Megazyme, County Wicklow, Bray, Ireland), 2 mM Ascorbic acid (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... A standard curve for β-1,3-glucan was constructed by using 0–50 mg ml-1 curdlan (Megazyme) dissolved in 0.1 M NaOH and also heating for 30 min at 50 °C in 1 N NaOH.
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Loss of p32 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitisbioRxiv - Molecular Biology 2020Quote: L-lactate levels were measured in serum or plasma samples (1:5 diluted in PBS) and in cell culture supernatants (1:10 diluted in PBS) according to manufacturer’s instructions (Megazyme, Wicklow, Ireland). Lactate level in cell culture supernatant were normalized to cell count.
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bioRxiv - Plant Biology 2020Quote: ... laminarin and were obtained from Megazyme.
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bioRxiv - Plant Biology 2020Quote: Starch content was determined using the Total Starch Assay Kit (Megazyme) with the following modifications ...
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bioRxiv - Plant Biology 2020Quote: Determination of the MLG content was performed using a mixed-linkage beta-glucan assay kit (K-GLU kit, Megazyme) with modifications ...
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bioRxiv - Systems Biology 2020Quote: ... Ammonium quantities were determined using an enzymatic kit according to the manufacturer’s instructions (Megazyme K-AMIAR). Quantified levels of the carbon source and ammonium were subsequently used to estimate the specific substrate uptake rates.
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bioRxiv - Plant Biology 2020Quote: ... 10 μL of the supernatant containing MLG-derived oligosaccharides was mixed with 10 μL β-Glucosidase (Megazyme) and incubated 15min at 50°C ...
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bioRxiv - Plant Biology 2020Quote: ... KOH-soluble fraction was then digested with licheninase (Megazyme) as described before ...
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bioRxiv - Plant Biology 2020Quote: ... cellopentaose and cellohexaose commercial standards (Megazyme).
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bioRxiv - Molecular Biology 2020Quote: The ForteBio Octet® RED96 interaction analyzer (ForteBio, United States) was used to detect the binding of XynA to the oligosaccharide substrates (cellobiose, xylohexaose and cellohexaose) (Megazyme, Ireland) and xylan (BWX ...
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bioRxiv - Physiology 2020Quote: ... Beta-glucan was analyzed using a Megazyme analysis kit (Mixed-linkage beta-glucan assay procedure/McCleary method, Megazyme International Ireland Ltd. ...
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bioRxiv - Physiology 2020Quote: ... The analysis of insoluble dietary fibre (IDF) and soluble dietary fibre (SDF) was completed using a Megazyme kit (Total dietary fibre assay procedure, Megazyme International Ireland Ltd. ...
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bioRxiv - Physiology 2020Quote: ... Ingredients were analyzed for total starch using the AOAC method 996.11 and the AACC method 76-13.01 using a Megazyme kit (Total starch assay procedure, Amyloglucosidase/α-amylase method, Megazyme International Ireland Ltd. ...
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bioRxiv - Cell Biology 2020Quote: Trehalose amount was measured using Trehalose Assay Kit (Product code: K-TREH, Megazyme Ltd.) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... glucose was measured with a commercial kit from (K-GLUHK, Megazyme).
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bioRxiv - Biochemistry 2020Quote: ... cellopentaose and cellohexaose were from Megazyme (Bray, Ireland). Sodium cacodylate ...
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bioRxiv - Microbiology 2020Quote: ... SBABN and glucomannan (Megazyme). All conditions were tested in quadruplicate ...
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bioRxiv - Microbiology 2020Quote: ... Sugar beet arabinan (Megazyme) was analyzed simultaneously ...
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bioRxiv - Microbiology 2020Quote: PFABN was enzymatically de-starched using amyloglucosidase and α-amylase (Megazyme). To do so ...
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bioRxiv - Plant Biology 2020Quote: ... All samples were centrifuged for 10 min at 4 °C and 13000 rpm and the supernatant was collected into a new tube for sugar quantifications that were performed using ©Megazyme Sucrose/D-Fructose/D-Glucose Assay Kit (K-SUFRG, ©Megazyme) with a few modifications ...
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bioRxiv - Cell Biology 2020Quote: Analyses of D-glucose and L-lactate concentrations from spent culture medium samples were performed using the D-Glucose-HK and L-Lactic acid kits from Megazyme following manufacturer's protocol ...
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bioRxiv - Microbiology 2020Quote: ... The first step involved manually adding 75 µl of 1% w/v Beechwood xylan (Megazyme) solution to a 96 well PCR plate (FLAT 96 WELL PCR PLATE ...
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bioRxiv - Microbiology 2020Quote: ... Transaminase activity was tested using an L-glutamic acid detection kit (K-GLUT, Megazyme Inc., Wicklow, Ireland), according to the manufacture’s microplate assay procedure with some modifications ...
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bioRxiv - Microbiology 2020Quote: ... and 3.5 U of pullulanase M1 from Klebsiella planticola (Megazyme), then desalted with AG® 501X8(D ...
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bioRxiv - Microbiology 2020Quote: ... 15µL of reaction sample were incubated 30 min at 58°C with 30 µL of 100 mM sodium citrate pH 4.6 containing whether 0.4 U of amyloglucosidase from Aspergillus niger (Megazyme) or no amyloglucosidase to then quantify the amount of free glucose and maltose after addition of 100 µL of a buffer (500 mM triethanolamine hydrochloride ...
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bioRxiv - Microbiology 2020Quote: ... The increase in absorbance at 340 nm after the supplementary addition of 1.2 U of hexokinase and 0.6 U of glucose-6-phosphate dehydrogenase (Megazyme) allowed us to estimate the amount of glucose units from a standard curve ...