Labshake search
Citations for Megazyme :
1 - 50 of 328 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2024Quote: ... and centrifuged to obtain the supernatant which was used for sucrose measurement using the sucrose/D-glucose assay kit (Megazyme) (10 ...
-
bioRxiv - Plant Biology 2024Quote: The total starch content of samples collected daily was determined using Total Starch Assay Kit (K-TSTA-100A, Megazyme, Ireland) as described in its instruction with modifications ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were analyzed using the L-Arabinose/D-Galactose Assay Kit (Megazyme) and the L-Fucose Assay Kit (Megazyme ...
-
bioRxiv - Microbiology 2024Quote: ... and the L-Fucose Assay Kit (Megazyme) per kit protocols for the microplate assay format ...
-
bioRxiv - Plant Biology 2024Quote: ... 100 mg flour was used and the content was masured using the Megazyme Total Starch Assay Kit (Megazyme; KTSTA-50A) following manufacturer’s method (three replicates per sample) ...
-
bioRxiv - Plant Biology 2024Quote: Cellulase digestions were performed by adding 5 U of endo-β-1,4-glucanase (Trichoderma longibrachiatum; Megazyme: E-CELTR) directly to the reaction mixture ...
-
bioRxiv - Plant Biology 2024Quote: Starch content was extracted from 50 mg of dry matter and analyzed using the “Total Starch” enzymatic kit (Megazyme International, County Wicklow, Ireland). Briefly ...
-
bioRxiv - Biochemistry 2024Quote: ... and 1 U of galactose dehydrogenase (GalDH, Megazyme, Wicklow, Ireland). The absorbance was monitored at 340 nm until equilibrium was reached ...
-
bioRxiv - Biochemistry 2024Quote: ... AZO-Carob Galactomannan were purchased from Megazyme (Ireland).
-
bioRxiv - Biochemistry 2024Quote: The kinetic parameters of the BT4241 GH2 β-galactosidase against disaccharide substrates was determined by the continuous monitoring of galactose release using a D-Gal detection kit (Megazyme International) at 37°C in 20 mM MOPS buffer ...
-
bioRxiv - Biophysics 2024Quote: ... another set of reaction was set up wherein 5U of endo-(1,4)-β-glucanase (E-CELTR; Megazyme) was added at the beginning of the synthesis reaction for the enzymatic degradations of the in vitro synthesized glucan ...
-
bioRxiv - Biochemistry 2024Quote: ... as well as AZCL substrates from Megazyme. Standards ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... trehalose was extracted and quantified using a Megazyme trehalose kit (Megazyme, Bray, Ireland) according to Santos and Ebert36 ...
-
bioRxiv - Microbiology 2024Quote: ... pectic galactan (Megazyme), xylan (carbosynth) ...
-
bioRxiv - Microbiology 2024Quote: ... arabinoxylan (Megazyme), pectic galactan (Megazyme) ...
-
bioRxiv - Microbiology 2024Quote: ... Solutions of arabinan (Megazyme), arabinoxylan (Megazyme) ...
-
bioRxiv - Microbiology 2024Quote: ... and 0.6 U of α1,2,3,4,6-L-fucosidase (Megazyme, E-FUCHS) for 3 h as positive controls for sialidase and fucosidase activity ...
-
bioRxiv - Biochemistry 2024Quote: ... For the holo form with GlcNAc2 (Megazyme O-CHI2), this construct crystallized in either P21212 or P212121 with either 2 or 4 molecules in the ASU and diffracted to a maximum resolution between 1.50 to 1.95 Å ...
-
bioRxiv - Biochemistry 2024Quote: ... For the holo form with GlcNAc3 (Megazyme O-CHI3), this construct crystallized in P21212 with 2 molecules in the ASU and diffracted to a maximum resolution of 1.70 Å.
-
bioRxiv - Physiology 2023Quote: ... Total starch in each diet was determined using a total starch analysis kit (amyloglucosidase/alpha-amylase method; Total Starch Assay Kit [AA/AMG], K-TSTA-50A, Megazyme, Wicklow, Ireland). Amounts of rapidly digestible starch (RDS) ...
-
bioRxiv - Plant Biology 2023Quote: ... isoamylase (Megazyme, Bray, Ireland) and 1 unit of Klebsiella planticola pullulanase (Megazyme ...
-
bioRxiv - Plant Biology 2023Quote: ... by using the primary antibody LM6 ([Anti-1,5-α-L-Arabinan] Antibody, Megazyme Ltd, Bray, Ireland) and a fluorescent marker (Alexa Fluor 568 goat anti-rat IgG (H+L) ...
-
bioRxiv - Plant Biology 2023Quote: ... disaccharides and CDs were purchased from Sigma-Aldritch (St. Louis, USA) whereas MLGs (TETB, TRIA, TRIB and TETC) and chitin oligosaccharides were purchased from Megazyme (Bray, Ireland). OGs were prepared as previously described (Pontiggia et al. ...
-
bioRxiv - Plant Biology 2023Quote: ... xyloglucanase (Megazyme, cat no. E-XEGP), endo 1,4 β-xylanase (Megazyme ...
-
bioRxiv - Plant Biology 2023Quote: ... pectate lyase (Megazyme, cat no. E-PCLYAN2), xyloglucanase (Megazyme ...
-
bioRxiv - Plant Biology 2023Quote: ... endo 1,4 β-xylanase (Megazyme, cat no. E-XYNACJ), endo 1,4 β-mannanase (Megazyme ...
-
bioRxiv - Plant Biology 2023Quote: ... and lichenase (Megazyme, cat no. E-LICHN) to 140 µl of 250 mM sodium citrate buffer (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2023Quote: Supernatant were used to measure acetate concentrations using Acetate assay kit (Megazyme Acetic Acid Assay Kit). The slope of the plot of acetate concentrations versus OD600 for all replicates (multiplied with the measured growth rate ...
-
bioRxiv - Biochemistry 2023Quote: 5 μL of CVF fluid (not centrifuged) was added to 95 μL of 10 mg mL-1 pullulan (Megazyme) in assay buffer (20 mM sodium acetate ...
-
bioRxiv - Biochemistry 2023Quote: ... 300 μL reactions were set up containing substrate (0.0048-10 mg mL-1 glycogen; 0.0012-1.25 mg mL-1 Pullulan (Megazyme); or 0.0048-1.25 mg mL-1 amylose in a final concentration of 2% DMSO) ...
-
bioRxiv - Plant Biology 2023Quote: ... E-XEGP (xyloglucan-specific endo-β-(1→4)-glucanase (Megazyme, Wicklow, Ireland) by adding 10 μl endoglucanase solution (0.4U/μl ...
-
bioRxiv - Biochemistry 2023Quote: ... and tamarind xyloglucan purchased from Megazyme. The model crystalline cellulose substrate Avicel PH-101 ...
-
bioRxiv - Microbiology 2023Quote: ... using the CellG5 and XylX6 kits (Megazyme, Wicklow, Ireland) as previously described (Ermoli et al. ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5 mg ml-1 linear β-1,2-glucan (Megazyme) in 300 μl final sample volume ...
-
bioRxiv - Microbiology 2023Quote: Growth on dehydroabietic acid and abietic acid was done in cultures of M9 medium containing 100 mg/L of dehydroabietic acid (Megazyme ∼90%) or abietic acid (Sigma ∼75% ...
-
bioRxiv - Microbiology 2023Quote: ... in a 24-well plate (6×4) using 100 mg/L of each resin acid: dehydroabietic acid (Megazyme ∼90%), abietic acid (Sigma ∼75%) ...
-
bioRxiv - Plant Biology 2023Quote: ... endo 1,4 β-mannanase (Megazyme, cat no. E-BMACJ) and lichenase (Megazyme ...
-
bioRxiv - Plant Biology 2023Quote: ... glucose was determined by the D-Glucose Assay Kit (GOPOD Format, Megazyme) using D-glucose standards as reference.
-
bioRxiv - Plant Biology 2023Quote: ... and 1 unit of Klebsiella planticola pullulanase (Megazyme, Bray, Ireland) for 16 hours at 42°C in 55 mM Sodium Acetate buffer pH 4.5 ...
-
bioRxiv - Plant Biology 2023Quote: Carbohydrate digestion assays were performed using either purified barley GBP (heterologously expressed in N. benthamiana) or barley BGLUII (Chandrasekar et al., 2022; available from Megazyme, E-LAMHV). Preparations of the fungal CW and EPS matrix were incubated overnight in sterile MilliQ water at 65°C prior to enzymatic digestion ...
-
bioRxiv - Plant Biology 2023Quote: ... Total starch content was measured using a Total Starch HK Assay Kit [K-TSHK] (Megazyme, Bray, Ireland). The assay followed the AOAC Official Method 996.11 for total starch determination (Englyst & Cummings ...
-
bioRxiv - Plant Biology 2023Quote: ... was added to 90 µL enzyme solution containing 50 mM sodium-phosphate buffer (pH4.5) and endo-β-1,4-galactanase from Aspergillus niger (1.3 units, Megazyme, Ireland) and incubated at 37°C for 12 h ...
-
bioRxiv - Plant Biology 2023Quote: ... Samples were digested with 1 U/mg DW sample of Aspergillus aculeatus endo-polygalacturonase M2 (Megazyme, Bray, Ireland) (Paterlini et al. ...
-
bioRxiv - Plant Biology 2023Quote: 10 mg of dried alcohol insoluble cell wall residues were incubated with 10U.ml-1 of endo-1,4-D-xylanase (E-XYLNP) from MEGAZYME previously desalted with centrifulgal filters (Amicon Ultra-0.5ml ...
-
bioRxiv - Microbiology 2023Quote: ... 10 min) and concentrations of lactate in the supernatants were determined using K-LATE kit (Megazyme).
-
bioRxiv - Plant Biology 2023Quote: ... Total starch content was calculated in accordance with the manufacturer’s (Megazyme, Bray, Ireland) instructions and data are presented as g/100g dry weight.
-
bioRxiv - Plant Biology 2023Quote: ... Both the thermostable α-amylase and amyloglucosidase were from the Total Starch Assay kit (K-TSTA, Megazyme, Bray). The digested sample was centrifuged to remove insoluble material ...
-
bioRxiv - Plant Biology 2023Quote: ... Soluble sugars were analysed using a kit (MEGAZYME, Bray, Ireland).
-
bioRxiv - Biochemistry 2023Quote: ... D-Glucose and D-Xylose release from biomass was quantified using commercial kits (Megazyme). Sugar concentration for each experiment was standardised with readings obtained from biomass- and enzyme-only controls ...
-
bioRxiv - Bioengineering 2023Quote: ... The hemicellulosic hydrolysate obtained after the second step was enzymatically treated with a GH11 from Neocallimastix patriciarum (Megazyme® Ireland) as detailed described elsewhere (Brenelli et al. ...