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Citations for Biosearch Technologies :
151 - 185 of 185 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Stellaris Probe Designer was used to design the smFISH probes and they were ordered from Biosearch Technologies (https://www.biosearchtech.com/). The probe labeling was performed by using equal volumes of each probe ...
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bioRxiv - Genomics 2021Quote: ... DAPI solution was aspirated and samples were washed once with wash buffer B (Biosearch Technologies Cat# SMF-WB1-20) for 5 min ...
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bioRxiv - Biophysics 2020Quote: ... Stellaris Probe Designer was used to design the smFISH oligonucleotide probes that were ordered from Biosearch Technologies (https://www.biosearchtech.com/). Each sRNA was labeled with 9 Alexa Fluor 647 probes while each ptsG mRNA was labeled with 28 CF 568 probes ...
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bioRxiv - Molecular Biology 2022Quote: ... Each sample was then washed twice with Stellaris RNA FISH Wash Buffer B (Biosearch Technologies Cat# SMF-WB1-20) with the second wash left to incubate for 30 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... smFISH was performed using SunTag-V4 probes (59) labeled with Quasar 670 dye (Stellaris RNA FISH probes, Biosearch Technologies), as described above ...
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bioRxiv - Molecular Biology 2023Quote: ... 56 Subsequent IVT reaction producing 5′-triphosphorylated RNAs was performed with NxGen T7 RNA Polymerase (Biosearch Technologies #30223-1). For segment 8 of IAV MEGAscript T7 Transcription Kit (Thermo #AM1333 ...
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bioRxiv - Microbiology 2023Quote: ... ∼5x107 cells were collected and total RNA was extracted using a MasterPure Yeast RNA purification kit (Biosearch Technologies, MPY03100) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The 70% ethanol was aspirated and the cells were washed once with 0.5 ml Stellaris RNA wash buffer A (Biosearch technologies). The cells were then incubated with 100 μl Stellaris hybridization buffer (Biosearch Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... The 70% ethanol was aspirated and the cells were washed once with 0.5 ml Stellaris RNA wash buffer A (Biosearch technologies). The cells were then incubated with 100 μl Stellaris hybridization buffer (Biosearch Technologies ...
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bioRxiv - Immunology 2022Quote: ... BSA (in PBS; ThermoScientific #77110) at 2.5 mg/ml was incubated with TNP-ε-Aminocaproyl-OSu (Biosearch Technologies #T-1030) in PBS with 20% dimethyl sulfoxide at a molar ratio of either 1:2 or 1:20 for 2 hours at room temperature while rotating ...
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bioRxiv - Microbiology 2023Quote: ... Single molecule FISH (smFISH) was performed using Stellaris™ RNA FISH protocol in Adherent Cells (Biosearch Technologies, Novato, CA, USA). After fixation ...
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bioRxiv - Genomics 2022Quote: ... and 2.5 µL of the reaction was transformed into 25 µL of Lucigen Endura ElectroCompetent Cells (Biosearch Technologies, Hoddesdon, UK). Before culture for maxiprep ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction was conducted using EconoTaq PLUS 2X Master mix (Cat no. 30035-1, Lucigen, LGC Biosearch technologies, USA) as follow ...
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bioRxiv - Genetics 2023Quote: ... The cell pellet was then resuspended with 100 µL per 1 million cells of QuickExtract (Pt#SS000035-D1 Biosearch Technologies). The hiPSCs and PBMCs were then run in a thermocycler for one round of three consecutive cycles ...
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bioRxiv - Cancer Biology 2021Quote: ... a set of oligonucleotides spanning the first intron of let-7-C was designed using the Stellaris Probe Designer software (Biosearch Technologies). The oligos were ordered from Biosearch Technologies labeled with Quasar-670.
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bioRxiv - Evolutionary Biology 2020Quote: ... respectively and a commercially available library of 48 probes was used to detect HOXA5 (cat # VSMF-2538-5) (Stellaris RNA FISH probes, Biosearch Technologies). Hybridization conditions and imaging were as previously described (Itzkovitz et al ...
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bioRxiv - Neuroscience 2019Quote: ... RNA in situ hybridisation in adult ventral nerve cords for the precursor RNA transcripts of miR-iab-4 was performed by designing 48 unique 20nt-probes labelled with Quasar 570 in the Stellaris platform from Biosearch Technologies, and using an adapted version of the protocol by Raj A ...
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bioRxiv - Molecular Biology 2019Quote: ... was incubated for 5 minutes and ACTB probe set was hybridized in for 16 hours at 37°C with Hybridization buffer (LGC Biosearch Technologies). After hybridization ...
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bioRxiv - Cancer Biology 2020Quote: ... A set of Stellaris RNA FISH Probes labeled with Cy5 dye specific to Hmrhl targeted transcript was purchased from Biosearch Technologies. Briefly ...
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bioRxiv - Microbiology 2021Quote: ... The abundance of SARS-CoV-2 genetic material was subsequently determined by quantitative PCR using a Taqman probe and primers (Biosearch Technologies). The primers and probe sequences for the were as recommended by BEI resources for compatability with synthetic RNA standards (# NR-52358).
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bioRxiv - Molecular Biology 2020Quote: ... The 70% ethanol was then removed and 1 mL Stellaris RNA FISH Wash Buffer A (Biosearch Technologies Cat# SMF-WA1-60) with 10% deionized formamide for 5 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA of each isolate was extracted using the MasterPure™ Yeast DNA Purification Kit (LGC Biosearch Technologies, Halle-Zoersei, Belgium), according to the manufactureŕs protocol ...
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bioRxiv - Cell Biology 2023Quote: Xist RNA was detected with Stellaris® RNA FISH (Human XIST with Quasar® 570 Dye, Biosearch Technologies, SMF-2038-1) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Genotyping of single colonies was performed after extraction of DNA with QuickExtract DNA Extraction Solution (Biosearch Technologies, catalog no. 76081-766) and PCR amplification with Q5® High-Fidelity 2X Master Mix (NEB ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA was extracted from 250,000 cells for each clonal cell line in 25 uL QuickExtractTM DNA Extraction Solution (BioSearch Technologies #QE09050) by thermocycling at 65°C for 6 minutes followed by 98°C for 2 minutes ...
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bioRxiv - Genomics 2024Quote: ... The assembled plasmid pool was purified and concentrated with AMPure XP SPRI Reagent and used for transformation of Electrocompetent Endura Cells (#60242-2, Biosearch Technologies) following manufacturer’s instructions and a previously published protocol47 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was extracted from samples at the two timepoints of the PCA experiment using MasterPure Yeast DNA Purification Kit (Biosearch Technologies).
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bioRxiv - Genomics 2023Quote: ... ligation and extension (GF) was made in molbio grade water over ice to a final concentration of 1x Ampligase Buffer (Biosearch Technologies, A1905B), 0.4 U/ uL RNaseH (Enyzmatics ...
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bioRxiv - Systems Biology 2023Quote: ... each cover glass was set on a droplet (cells facing down) consisting of 45 μL hybridization buffer (Biosearch Technologies, SMF-HB1-10) and 1 μL of the duplex smiFISH probes (MS2-transcript-binding probe mix + FLAP-Y-binding region annealed to FLAP-Y-Cy5 ...
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bioRxiv - Developmental Biology 2023Quote: ... Probes against soti and wa-cup transcripts were designed using the Stellaris® RNA FISH Probe Designer (Biosearch Technologies, Inc., Novato, USA) available online at www.biosearchtech.com/stellarisdesigner.
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bioRxiv - Neuroscience 2020Quote: ... Hybridization followed the manufacturer’s instructions (http://www.biosearchtech.com/stellarisprotocols) and was performed at 37°C for 16h in Stellaris RNA FISH hybridization buffer (Biosearch Technologies Cat# SMF-HB1-10) containing unc-8 probe at 1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... 3 ug of total RNA from cultured keratinocytes or fibroblasts was treated without (control) or with 10 units of RNase R in 10X RNase R reaction buffer (LGC Biosearch Technologies, Hoddesdon, UK). Treatment was conducted at 37°C for 12 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μL of the TdT-labeled probes was added to 98 μL of Stellaris RNA FISH Hybridization Buffer (Biosearch Technologies Cat# SMF-HB1-10); 100 μL of this mixture was dotted onto the Parafilm in the humidifying chamber ...
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bioRxiv - Molecular Biology 2021Quote: ... Excess buffer was removed and 100 uL of freshly prepared Hybe Buffer Mixture was added (for two color in situs: 85.5 uL of Stellaris® RNA FISH Hybridization Buffer (Biosearch Technologies; Cat #: SMF-HB1-10); 9.5 uL deionized formamide ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 uL of freshly prepared Hybe Buffer Mixture was added (for two color in situs: 85.5 uL of Stellaris® RNA FISH Hybridization Buffer (Biosearch Technologies; Cat #: SMF-HB1-10); 9.5 uL deionized formamide ...