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Citations for Biosearch Technologies :
201 - 250 of 795 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Fixed worms were stained at 46 °C overnight using FISH probes conjugated to the red Cal Fluor 610 fluorophore (Biosearch Technologies), targeting microsporidia ribosomal RNA (81 ...
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bioRxiv - Immunology 2023Quote: ... Fixed worms were washed and then stained at 46 °C overnight using FISH probes conjugated to the red Cal Fluor 610 fluorophore (Biosearch Technologies), targeting Orsay virus RNA1 and RNA2 (80 ...
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bioRxiv - Developmental Biology 2023Quote: ... Custom 20 nt oligonucleotides with a 3’NHS ester modification were obtained from Biosearch Technologies, conjugated to either Atto 565 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... with 100 U of NxGen RNase inhibitor (LGC Biosearch technologies, 30281), 2 U of beta-agarose I (NEB ...
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bioRxiv - Systems Biology 2023Quote: ... All oligos were synthesized with a 3’ amine modification (LGC Biosearch Technologies). The oligos against a given gene (oligo set ...
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bioRxiv - Immunology 2023Quote: ... NP(19)-BSA for high affinity or NP(>20)-BSA for all affinities (N-5050L, N-5050H, Biosearch Technologies)) in 25 μl PBS ...
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bioRxiv - Immunology 2023Quote: ... or NP-LPS (N-5065, Biosearch Technologies) for T-independent (TI ...
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bioRxiv - Immunology 2023Quote: ... groups of WT (CD19WT/WT Rab8aflox/flox) and Rab8a-/- (CD19WT/Cre Rab8aflox/flox) females were immunised with NP40-FICOLL (F-1420, Biosearch Technologies) or NP-LPS (N-5065 ...
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bioRxiv - Immunology 2023Quote: ... or NP31-KLH (N-5060, Biosearch Technologies) for T-dependent (TD ...
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bioRxiv - Cell Biology 2023Quote: ... 2μl of the probe/flap hybridization product (0.833µM) or smFISH probe was added to 100μl of smFISH hybridization buffer (Biosearch Technologies). A hybridization chamber was prepared using an empty 15cm cell culture plate ...
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bioRxiv - Cell Biology 2023Quote: ... the glass coverslips were transferred to a fresh 6-well plate with the cell side up and incubated with Wash Buffer A (Biosearch Technologies) and Hoechst stain (1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... Hoechst stain was washed with Wash Buffer B (Biosearch Technologies) at room temperature in the dark for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... and Wash B Buffers were purchased from Biosearch Technologies. Hoechst stain was purchased from AnaSpec ...
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bioRxiv - Cell Biology 2023Quote: smFISH probes for Firefly luciferase were obtained from Biosearch Technologies. The probes were labeled with Quasar 570 dye ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We then designed RNA-probes for our target sequences using the Stellaris RNA FISH platform (Biosearch Technologies) with the custom probe design service following their recommendations for probe design.
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bioRxiv - Developmental Biology 2023Quote: ... Probes against soti and wa-cup transcripts were designed using the Stellaris® RNA FISH Probe Designer (Biosearch Technologies, Inc., Novato, USA) available online at www.biosearchtech.com/stellarisdesigner.
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bioRxiv - Biophysics 2023Quote: All oligonucleotides used in this work (Table S2) were synthesized using a MerMade 6 instrument (LGC, Biosearch Technologies) at 1 μmol scale ...
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bioRxiv - Cancer Biology 2023Quote: ... was obtained from Biosearch Technologies (Petaluma, CA) and diluted in sterile deionized water ...
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bioRxiv - Developmental Biology 2023Quote: ... Genotyping PCR were done by using KASP genotyping assay (Biosearch Technologies, Hoddesdon, United Kingdom). A similar workflow was used in genotyping adult carrier fish.
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bioRxiv - Immunology 2023Quote: ... 10 μg/ml trinitrophenyl (TNP)-specific human IgG1-4 (clone 7B4) were incubated with 5 μg/ml TNP-conjugated BSA (TNP-33-BSA, BioSearch Technologies) in PBS for 3 hours gently shaking at 60 rpm ...
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bioRxiv - Genomics 2023Quote: ... 50 µl of RNase-inhibitor (Biosearch technologies, 30281-1)) which was added to nuclei suspension ...
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bioRxiv - Genomics 2023Quote: ... 10 x 200 µL aliquots of extraction buffer (Dissociation Buffer, 1% Kollidon VA64, 1% Triton X100, 0.01% BSA, 666 units/mL RNase-inhibitor (Biosearch technologies, 30281-1)) were dispensed onto the puck for a total volume of 2 mL ...
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bioRxiv - Cell Biology 2023Quote: ... Custom probe libraries were designed utilizing the Stellaris FISH probe designer software based on sequence to hybridize with a desired coding RNA (Biosearch Technologies, Inc., Petaluma, CA) and were coupled to Cy5 ...
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bioRxiv - Cancer Biology 2023Quote: Library amplification: E.cloni electrocompetent bacteria (Biosearch Technologies) were thawed on ice for 20 min prior to addition of 1 μL of Gibson reaction and electroporation (1800 V ...
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bioRxiv - Molecular Biology 2023Quote: Stellaris RNA FISH probes were designed against the first 40kb of Airn utilizing the Stellaris RNA FISH Probe Designer (LGC, Biosearch Technologies, Petaluma, CA) available online at www.biosearchtech.com/stellarisdesigner (version 4.2 ...
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bioRxiv - Molecular Biology 2023Quote: ... coli EPI300 cells (LGC Biosearch Technologies, UK). Sequences of all obtained plasmids were confirmed using Sanger sequencing and are available form authors upon request.
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bioRxiv - Immunology 2023Quote: ... mice were immunized with 100ug of NP(24)-CGG (Cat. N5055C-5, Biosearch technologies) emulsified in alum (Cat ...
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bioRxiv - Immunology 2023Quote: ... 100 μg or 20 μg NP-OVA (Biosearch Technologies, 20 μg for recall) mixed with 2 μg LPS (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from cells at exponential (Fig. 3C) or stationary (Fig. 1C) phase using the MasterPure Yeast RNA Purification Kit including a DNase treatment step (Biosearch Technologies), and converted to cDNA using random primers and the RevertAid Reverse Transcriptase kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5X sample premixes were created with 2.4 μL of 10X T7 Buffer (Biosearch Technologies F88905-1, Hoddesdon, United Kingdom), 3.2 μL of 7.5X sample buffer ...
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bioRxiv - Molecular Biology 2023Quote: Assay mixes were prepared in 16 μL volumes for each unique crRNA target consisting of 1 μL of 50 U/μL NxGen T7 Polymerase (Biosearch Technologies 30223-1), 2 μL of 800 nM LwaCas13a (Genscript) ...
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bioRxiv - Cell Biology 2023Quote: ... Ligations were transformed into Endura Electrocompetent Cells (LCG Biosearch Technologies, Middlesex, UK) using a Gene Pulser II (Bio-Rad ...
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bioRxiv - Genetics 2023Quote: ... The probes were designed by Biosearch Technologies. The sequences of the new linc-133 probes can be found in Table S2 ...
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bioRxiv - Neuroscience 2023Quote: ... After electroporation transformation with Endura Electrocompetent Cells (60242-1, Biosearch Technologies, USA), the cells were plated into Nunc™ Square BioAssay Dishes (Catalog number ...
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bioRxiv - Developmental Biology 2023Quote: smFISH probes (32 probes per gene) were designed using the Stellaris Probe Designer (Biosearch Technologies) and ordered as unlabelled DNA oligos ...
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bioRxiv - Cell Biology 2023Quote: Probe design: DNA probes coupled to CAL Fluor Red 590 (Stellaris Biosearch Technologies, synthesized by BioCat) were used for smFISH ...
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bioRxiv - Biochemistry 2023Quote: ... NxGen phi29 (Biosearch Technologies, 30221), iododeoxyuridine (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... 50μg NP(30-39)-CGG (Biosearch Tech, cat: N-5055D-5) in PBS was mixed with Imject Alum (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: Mice were injected intraperitoneally with 50μg of 4-hydroxy-3-nitrophenylacetyl (NP)-CGG (BioSearch Technologies) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... We also propagated SCN1A into CopyCutter EPI400 cells (formerly Lucigen, now BioSearch Technologies, catalog # C400CH10) for low-copy growth in absence of CopyCutter Induction Solution ...
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bioRxiv - Molecular Biology 2023Quote: Oligonucleotides were synthesized by solid-phase phosphoramidite chemistry on a MerMade12 (Biosearch Technologies, Novato, CA) or Dr Oligo 48 (Biolytic ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted with MasterPure™ Complete DNA & RNA Purification Kit (MC89010, LGC Biosearch Technologies). Isolates were grown overnight on blood agar (PP0120-9090 ...
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bioRxiv - Immunology 2023Quote: ... by one or two intraperitoneal (i.p.) injections of 100 µg of 4-hydroxy-3-nitrophenylacetyl hapten (NP) conjugated to ovalbumin (NP-ova, Biosearch Technologies, Novato, CA), emulsified in 100 µL Imject® alum (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... All- or high-affinity hapten-specific Ab were measured using plates coated with NP20-BSA or NP2-PSA (Biosearch Technologies), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... Twenty microlitres of total RNA was digested with 2.5 units of RNase R (Biosearch Technologies, RNR07250) for one hour at 37°C to remove linear RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... Nucleofected clones were isolated by limiting dilution and indel-containing clonal populations were screened by extracting gDNA using Quick Extract (Biosearch Technologies) for PCR amplification using primers targeting up and downstream the Cas9 cut-site ...
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bioRxiv - Genomics 2023Quote: ... Thirty μL of the eluate were digested using the Plasmid-Safe ATP-dependent DNase (Biosearch Technologies, Hoddesdon, UK) for 17 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: mRNA was isolated using Master Pure Complete DNA&RNA purification kit (Biosearch technologies) according to the manufacturer’s instructions except that scale was increased 2 times ...
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bioRxiv - Biochemistry 2023Quote: ... The ∼105 nt cDNAs were separated from free RT primers on a 15% TBE-Urea gel and circularized using CircLigaseII ssDNA Ligase (Biosearch Technologies; CL9021K). PCR was carried out using forward primer NI-798 ...
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bioRxiv - Cell Biology 2023Quote: RNA FISH experiments were done using Stellaris oligonucleotide probes labeled with Quasar 570 Dye according to the manufacture’s protocol (LGC Biosearch Technologies). Sequences of the probes are listed in Supplementary Table 2.