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Citations for SignaGen Laboratories :
51 - 77 of 77 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... lentiviral vectors were cotransfected with viral packaging vectors into 70% confluent HEK293TN cells using PolyJet (SignaGen Laboratories, SL100688). Media were changed into DMEM containing 1% FetalClone-II serum (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... lentivirus packaging plasmids into 8 million HEK293T cells in a 10-cm dish with PolyJet (SignaGen Laboratories, SL100688). For each individual sgRNA ...
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bioRxiv - Cancer Biology 2023Quote: ... and envelop plasmids pMD2.G (a gift from Dr. Maciej Olszewski) into HEK-293T cells using lipoD293 (SignaGen Laboratories) as transforming reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... COMMA1D cells were then transfected with INSR knock-out plasmid using 15 µL PolyJet transfection reagent (SignaGen Laboratories #SL100688). Following transfection ...
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bioRxiv - Neuroscience 2020Quote: pCAG-eCas9-GFP-U6-Rpsa-gRNA plasmid was transfected into mouse Neuro-2a cells using PolyJet transfection reagent (SignaGen Laboratories), and Genomic DNA was isolated and subjected to PCR to amplify the 433 bp fragment containing gRNA target sequence using Q5 High Fidelity DNA polymerase (NEB ...
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bioRxiv - Synthetic Biology 2022Quote: ... HEK293T and HEK293T-NnSULT1C1 cells were transfected with pAcBacw.tR4-sTyrRS/GFP* with Polyjet In Vitro DNA Transfection Reagent (SignaGen Laboratories) in the presence or absence of the indicated concentration of sTyr ...
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bioRxiv - Molecular Biology 2022Quote: ... Transfection of HEK293T cells was performed by mixing plasmid DNA with PolyJet™ In Vitro DNA Transfection Reagent (SignaGen Laboratories) and incubation with cells for 3 h ...
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bioRxiv - Genetics 2021Quote: ... plasmids for either ABCC5 or ABCC12 constructs were transfected into cell lines using PolyJet transfection reagent (SignaGen Laboratories, cat. number SL100688). Previously ...
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bioRxiv - Cancer Biology 2020Quote: ... SiHa cells at 24 h of cell passage were transfected with 40 nM of siRNA by LipoJet In Vitro Transfection Kit (Ver. II) (SignaGen Laboratories). Total protein extracts and total RNA were prepared 48 h after the transfection.
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bioRxiv - Cancer Biology 2023Quote: ... packaging plasmids psPAX2 and enveloped plasmids pMD2.G (a gift from Dr. Maciej Olszewski) into HEK-293T cells using lipoD293 (SignaGen Laboratories) as transforming reagent ...
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bioRxiv - Microbiology 2024Quote: Cell extracts for oligo pulldown assays were isolated from HEK293T cells transfected with an ORF57-expressing vector (pVM7) using LipoD293 transfection reagent (SignaGen Laboratories). Twenty-four hours after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... plasmid expressing sfGFP-TGA-Strep-6xHis-Strep was co-transfected with a plasmid expressing 4 copies of the original or optimized (Opt) ACE-tRNA expression cassettes into HEK293T cells at 75% confluency using Calfectin (SignaGen Laboratories) according to standard protocols ...
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bioRxiv - Genomics 2023Quote: ... packaging plasmids were cotransfected into 8 million HEK293T cells in a 10-cm dish supplemented with 36 μl PolyJet (SignaGen Laboratories, SL100688). The medium was replaced 12 hours after transfection and harvested every 24 hours thereafter for a total of three harvests ...
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bioRxiv - Genomics 2023Quote: ... we transfected two million MCF7-dCas9/RT cells with 2000 ng of ngRNA/pegRNA plasmid containing an EGFP marker using PolyJet (SignaGen Laboratories, SL100688). Five days after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... AAV plasmids containing the target genes were co-transfected with pAAV2 (pACG2)-RC triple mutant (Y444, 500, 730F) and the pHelper plasmid (StrateGene) into HEK293T cells by the PolyJet (SignaGen Laboratories, SL100688) transfection reagent ...
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bioRxiv - Cancer Biology 2024Quote: Lentivirus was produced according to the “General Protocol for Generation of lentivirus in 293T cells” using “PolyJet™ In Vitro DNA Transfection Reagent” (Signagen Laboratories). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... a 2:1 mixture of AAV1-CAG-DIO-Ace2N-4AA-mNeon (Virovek) and AAV2-Synapsin-tdTomato (1.23 × 1012 vg ml−1; SignaGen Laboratories) were injected at the following coordinates ...
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bioRxiv - Cancer Biology 2019Quote: MDA-MB-231 cells were transfected with gene-specific siRNA or control siRNA (20 nM) using PepMute Plus transfection reagent (SignaGen Labs, Rockville, MD). After 18 hours of transfection ...
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bioRxiv - Microbiology 2020Quote: ... DsiRNAs were transfected into Vero cells growing on 35 mm glass bottom dishes or on standard 35 mm dishes using PepMute transfection reagent (SignaGen Laboratories, Rockville, MD) according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... 1X 150 mm dish of HEK293 cells were transfected with either FLAG-HA-YTHDC1 or FLAG-ZFC3H1 (1-1233) using Lipo293T DNA in vitro transfection reagent (SignaGen Laboratories). 18 to 24 hours post transfection the cell pellets were trypsinized ...
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bioRxiv - Molecular Biology 2020Quote: All cells were plated 24 h before transfection and transfected at a confluency of 70-80% using GenJet-OS in vitro transfection reagent for U2OS cells (SignaGen Laboratories, Gaithersburg, MD, USA) or JetPRIME for 293T cells (PolyPlus ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with plasmids using GenJet™ in vitro transfection reagent (Ver. II) pre-optimized and conditioned for transfecting Neuro2A and BHK-21 cells respectively (SignaGen laboratories, Ijamsville, MD) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... the HEK-293T cells were transfected with the expression vectors and package vectors (psPAX2 and pMD2.G) by PolyJet (SignaGen laboratories, Cat. No. SL100688). After 48 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... the expression vectors were transfected together with package vectors (psPAX2 and pMD2.G) into HEK-293T cells by PolyJet (SignaGen laboratories, Cat. No. SL100688). After 48 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using LipoD293™ Transfection Reagent (SignaGen Laboratories.) Conditioned media containing virions was harvested after 24 and 48 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Viruses were generated by co-transfecting 107 cells plated the previous day in 15 cm dishes with 30 µg of total plasmid DNA (pNLX.Luc.R-.ΔAvrII and a vesicular stomatitis virus G envelope expressor at 6:1 ratio using PolyJet™ DNA transfection reagent; SignaGen Laboratories). Two days post-transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... B16-F1 cells were transfected with 1 μg DNA of pCMV3 vector encoding IL-6 or the empty control pCMV3 vector (EV) using PolyJet (SignaGen Laboratories, MD, USA) following the manufacturer’s instructions ...