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Citations for SignaGen Laboratories :
1 - 50 of 77 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Viral injection of the recombinant adeno-associated virus expressing Cre recombinase under the control of human synapsin promoter (rAAV-hSyn-Cre; from SignaGen Laboratories, Rockville, MD) was done by bulk regional viral injection to the visual cortical area of neonatal Cre dependent Gs-DREADD mice at p1 ...
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bioRxiv - Cell Biology 2023Quote: ... pMD2.G and psPAX2 in 2:1:2 ratio with PolyJet transfection reagent (SignaGen Laboratories). Media were harvested two days later and added to recipient cells with 1 μg/ml polybrene (Sigma ...
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bioRxiv - Systems Biology 2023Quote: ... 15 μg of Strep-tagged plasmids was combined with PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 μg:μl ratio of plasmid:transfection reagent ...
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bioRxiv - Biophysics 2021Quote: ... Up to 15 μg of individual Strep-tagged expression constructs (normalized to 15 μg with empty vector as needed) was complexed with PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 μg:μl ratio of plasmid to transfection reagent based on manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2019Quote: Cre recombinase was expressed in organoids using Recombinant Adenovirus Ad-Cre-GFP from SignaGen Laboratories (#SL100706), which co-expresses GFP as a marker ...
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bioRxiv - Neuroscience 2021Quote: ... and transfected at 80 % confluence with 1 μg of LRRK2 constructs (and 100 ng of HA-tagged vps35 constructs where indicated) and 3 μl of LipoD293™ Transfection Reagent (SignaGen Laboratories) per well of a 12-well plate overnight ...
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bioRxiv - Molecular Biology 2021Quote: Recombinant AAV constructs were packaged in AAV6 capsids and purified by SignaGen Laboratories (#SL100865, >1013 VG/mL). Production of AAV-CMV-unreg-CUG1 was attempted on three occasions but titers >109 VG/mL could not be obtained ...
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bioRxiv - Immunology 2020Quote: 293T cells were cultured in complete DMEM (10% HI-FBS, 1x Pen/Strep) and then transfected using the LipoD293 Transfection reagent (SignaGen Laboratories, cat. SL100668) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... HEK cells were transfected with 2-3 μg of the designated construct or empty vector using the PolyJet (SignaGen Laboratories, SL100688).
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bioRxiv - Neuroscience 2020Quote: ... LV-Syn-DIO-GluN1-2A-tdTomato (Vigene Biosciences, 2 × 109 transducing units (TU)/mL) and LV-Syn-DIO-mTagBFP2-2A-GluN2B (SignaGen Laboratories, 1 × 109 TU/mL). For control fluorophores ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with fluorescent reporter constructs (1 μg total DNA/well, 6 well plate) using GenJet (SignaGen Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: The lentiviral vector for eGFP under control of the human EF1α-promoter was purchased from SignaGen Laboratories (Gaithersburg MD, USA). All other lentiviral vectors were made in the department of Prof ...
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bioRxiv - Microbiology 2024Quote: ... 5 ×105 cells seeded in a 6-well plate were transfected with 40 nM SMARTpool human siRNAs (Horizon) or non-targeting (siNT) control siRNA (Horizon) using LipoJet In Vitro Transfection Kit (Ver. II) (SignaGen Laboratories). Twenty-four hours after the first transfection ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells at 0.25 × 106 were plated into 6-well plates 24 h before the first siRNA transfection with 40 nM of the ON-TARGETplus human siRNA SMARTpool (Horizon Discovery Biosciences) using LipoJet (SignaGen Laboratories, Frederick, MD) as recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using LipoD293™ transfection reagent (SignaGen Laboratories; Cat# SL100668) at a 1:2.5 DNA:Lipo ratio (2.5ug transfer plasmid + 2.5ug psPAX2 + 1.0ug pMD2.G and 15ul lipoD per plate) ...
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bioRxiv - Immunology 2020Quote: ... All transient transfections for SARS-CoV-2 infection experiments were performed using LipoJet transfection reagent (Signagen Laboratories). siRNAs targeting IFITM1 (s16192) ...
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bioRxiv - Biochemistry 2020Quote: HeLa cells were transfected with a 1:1:1 mix of gRNA1 and gRNA2 vectors together with the donor plasmid using PolyJet transfection reagent (SL100688, SignaGen Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... into 293T cells using PolyJet (SignaGen Laboratories #SL100688) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected by LipoD293 (SignaGen laboratories #SL100668) at DIV1 ...
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bioRxiv - Cell Biology 2021Quote: ... Huh7 cells were transfected with GenJet™ (SignaGen Laboratories), and HEK 293T cells were transfected with a 1:3 ratio of DNA:polyethylenimine (PEI ...
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bioRxiv - Neuroscience 2023Quote: ... into 70% confluent HEK293TN cells using PolyJet (SignaGen Laboratories). Media was changed into DMEM containing 1% FetalClone-II serum (Hyclone ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA transfections for HEK cells used PolyJet (SignaGen Laboratories), whereas MEF cells used TransIT-X (Mirus ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated in a 1:1 mixture of DNA/Polyjet DNA transfection reagent for 5-18 hours according to the manufacturer’s protocol (SignaGen Laboratories). Expression of plasmid DNA was selected using G418 ...
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bioRxiv - Genomics 2020Quote: ... were transformed into 293T cells using PolyJet (SignaGen Laboratories #SL100688) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... cells were transiently transfected using PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 ratio of plasmid to transfection reagent ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected using PolyJet transfection reagents (SignaGen Laboratories, Rockville, MD). 8×105 cells were plated on a 3-cm plate overnight ...
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bioRxiv - Cancer Biology 2020Quote: ACLY was transiently transfected in HEK293T cells using Polyjet (SignaGen Lab) with pLenti vector and pLenti-GIII-CMV-Human-ACLY HA-tagged constructs (Abmgood ...
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bioRxiv - Cancer Biology 2024Quote: ... using CalFectinTM Cell Transfection Reagent (SL100478, SignaGen Laboratories; Frederick, MD; USA) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... using a 1:3 mixture of LipoD293 (SignaGen Laboratories SL100668). 48 hours later ...
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bioRxiv - Cell Biology 2021Quote: ... NRK or PC12 cells were transfected using Polyjet (SignaGen Laboratories; Frederick, MD), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and transfected into HEK293T cells using LipoD293 (SignaGen Laboratories, catalog no. SL100668). After 48 h ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were co-transfected using GenJet™ (cat# SL00488, SignaGen Laboratories) lipofection with Tet-pLKO-puro (Addgene plasmid #21915 ...
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bioRxiv - Neuroscience 2021Quote: ... N2a or tsA-201 cells were transfected using either PolyJet™ (SignaGen Laboratories) or Fugene® 6 (Promega ...
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bioRxiv - Neuroscience 2023Quote: HEK293T cells were transfected with indicated plasmids with GenJet Transfection Reagent (SignaGen Laboratories), and at 48 hr cells were lysed in ice-cold RIPA buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Mutant plasmids were directly transfected into cell lines using GeneJet transfection reagent (SignaGen Labs) or packaged in 293T for lentiviral infection.
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bioRxiv - Microbiology 2020Quote: 293T cells were transiently transfected with GenJetTM In Vitro DNA Transfection Reagent (SignaGen Laboratories). Luciferase reporter assays were performed 24 h after DNA transfection ...
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bioRxiv - Neuroscience 2020Quote: ... cells were transduced at MOI of 10 with ADV-CMV-FOXF2-mCherry (SignaGen Laboratories, SL100701), ADV-CMV-OLIG2-mCherry (SignaGen Laboratories ...
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bioRxiv - Genomics 2021Quote: ... plasmids into 4 million HEK293T cells in a T25 flask with PolyJet (SignaGen Laboratories, SL100688). Media was replaced 12 h after transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... siRNA transfection was performed in cells using GeneMut siRNA transfection reagent (SignaGen Laboratories cat. SL100568) (Rockville ...
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bioRxiv - Biochemistry 2023Quote: ... Transfection of HEK293T or HCT116 cells was carried out using LipoD293 (SignaGen Laboratories, Rockville, MD) or FuGENE HD (Promega ...
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bioRxiv - Cell Biology 2023Quote: Plasmids were transfected into 40-50% confluent cells using PloyJet reagent (SignaGen Lab MD, USA). siRNAs were transfected with the siRNA transfection reagent INTERFERin® (Polyplus ...
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bioRxiv - Genetics 2021Quote: ... then the cells were transfected with plasmids using a LipoD293 DNA transfection reagent (SignaGen Laboratories, SL100668) at 24–48 hours before the imaging studies ...
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bioRxiv - Biochemistry 2020Quote: HEK-293FT cells were transfected approximately 48 hours before imaging using CalFectin (SignaGen Laboratories, catalog number #SL100478). A plasmid ratio of 3:1 for the SunTag:scFv-sfGFP/OsTIR1 plasmids was used ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with plasmid DNA using PolyJet DNA In Vitro Transfection Reagent (Signagen Laboratories, Gaithersburg, USA) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: HEK-293T and A549 cells were transfected with LipoJet™ In Vitro Transfection Kit (SignaGen Laboratories, SL100468) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2021Quote: ... cells were transiently transfected with Lipojet reagent (LipoJet™ In Vitro Transfection Kit, SignaGen Lab. Cat # SL100468) to introduce a full-length TTX-resistant human SCN2A WT or L1342P variant (gifts from Dr ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were transfected using GenJet™ In Vitro DNA Transfection Reagent (SignaGen Laboratories, catalog # SL100489-OS) for U2OS Cells as instructed ...
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bioRxiv - Microbiology 2021Quote: ... and 1 ng pCMV-IFNL2-genomic or pCMV-IFNL2-cDNA using PolyJet transfection reagent (SignaGen labs) according to the manufacturer’s DNA transfection protocol ...
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bioRxiv - Neuroscience 2019Quote: ... HEK293 cells were transfected with GFP-TDP-43 by PolyJet™ DNA in vitro transfection reagent (Signagen Laboratories). Cells were seeded into a 6-well plate at a density of 5 × 105 cells/well and incubated overnight ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transfected using Genefect (Alkali Scientific, Fort Lauderdale, FL) or LipoJet transfection reagents (Signagen Laboratories, Rockville, MD), according to each manufacturer’s protocol ...