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Citations for SignaGen Laboratories :
101 - 150 of 190 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... packaging plasmid (Δ 8.9) and envelope (VSVG) vectors using Lipo293T DNA in vitro transfection reagent (SignaGen Laboratories) according to the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... U2OS cells were transfected with the appropriate amount of DNA plasmid according to the manufacturer’s protocol using GenJet U2OS DNA in vitro transfection reagent (SignaGen Laboratories) for 18 to 24 hrs.
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bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out using PolyJet™ (SignaGen labs) according to the manufacturer’s DNA transfection protocol and cells were harvested 24 hr post transfection ...
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bioRxiv - Genetics 2021Quote: ... then the cells were transfected with plasmids using a LipoD293 DNA transfection reagent (SignaGen Laboratories, SL100668) at 24–48 hours before the imaging studies ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells at 0.25 × 106 were plated into 6-well plates 24 h before the first siRNA transfection with 40 nM of the ON-TARGETplus human siRNA SMARTpool (Horizon Discovery Biosciences) using LipoJet (SignaGen Laboratories, Frederick, MD) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... using PolyJet DNA Transfection Reagent (SignaGen Laboratories, Cat# SL100688 Rockville, MD) as per the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... AAV6 viral particles were produced and purified by SignaGen Laboratories (Rockville, MD).
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bioRxiv - Microbiology 2021Quote: ... the corresponding plasmids were transfected using Poly Jet™ transfection reagent (SignaGen Laboratories, SL100688) after the 293T cells had been grown to 80% confluence for 18-24 h before treatments ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transfected using Genefect (Alkali Scientific, Fort Lauderdale, FL) or LipoJet transfection reagents (Signagen Laboratories, Rockville, MD), according to each manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... N2a or tsA-201 cells were transfected using either PolyJet™ (SignaGen Laboratories) or Fugene® 6 (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with fluorescent reporter constructs (1 μg total DNA/well, 6 well plate) using GenJet (SignaGen Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: 1×106 cells/6cm2 tissue culture plate were transfected with TMD insertion reporters (2.5 μg total DNA/6cm2 plate) using GenJet (SignaGen Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... the PolyJet Transfection Reagent (SignaGen Laboratories) was used per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected using PolyJet transfection reagents (SignaGen Laboratories, Rockville, MD). 8×105 cells were plated on a 3-cm plate overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Huh7 cells were transfected with GenJet™ (SignaGen Laboratories), and HEK 293T cells were transfected with a 1:3 ratio of DNA:polyethylenimine (PEI ...
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bioRxiv - Microbiology 2021Quote: Transfections were performed with GenJet (SignaGen Laboratories) or Lipofectamin 2000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... plasmids were incubated with 3 μl CalFectin (SignaGen laboratories, Rockville, MD, USA) in Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... II (SignaGen Laboratories). In all cases ...
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bioRxiv - Cell Biology 2021Quote: ... The SH-SY5Y neuroblastoma cells were transfected with 10 ug of plasmid DNAs when 60% confluence in 100-mm dishes using transfection reagents GenJet II (SignaGen Laboratories, Rockville ...
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bioRxiv - Neuroscience 2021Quote: ... were carried by SignaGen Laboratories (Johns Hopkins University, USA). A European patent application has been filed for the AAV- PHP.EB vectors and is currently pending.
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bioRxiv - Cancer Biology 2021Quote: ... Transient transfections of plasmids were performed by using LipoD293™ In Vitro DNA Transfection Reagent (SignaGen Laboratories). Transient transfections of SMARTPool SGK3 siRNA (L-004162-00-0005 ...
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bioRxiv - Genomics 2021Quote: ... lentivirus packaging plasmids into 8 million HEK293T cells in a 10-cm dish with PolyJet (SignaGen Laboratories, SL100688). For each individual sgRNA ...
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bioRxiv - Genomics 2021Quote: ... plasmids into 4 million HEK293T cells in a T25 flask with PolyJet (SignaGen Laboratories, SL100688). Media was replaced 12 h after transfection ...
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bioRxiv - Physiology 2021Quote: ... cells were transiently transfected with Lipojet reagent (LipoJet™ In Vitro Transfection Kit, SignaGen Lab. Cat # SL100468) to introduce a full-length TTX-resistant human SCN2A WT or L1342P variant (gifts from Dr ...
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bioRxiv - Cell Biology 2021Quote: ... NRK or PC12 cells were transfected using Polyjet (SignaGen Laboratories; Frederick, MD), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and transfected into HEK293T cells using LipoD293 (SignaGen Laboratories, catalog no. SL100668). After 48 h ...
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bioRxiv - Molecular Biology 2021Quote: Recombinant AAV constructs were packaged in AAV6 capsids and purified by SignaGen Laboratories (#SL100865, >1013 VG/mL). Production of AAV-CMV-unreg-CUG1 was attempted on three occasions but titers >109 VG/mL could not be obtained ...
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bioRxiv - Biophysics 2021Quote: ... Up to 15 μg of individual Strep-tagged expression constructs (normalized to 15 μg with empty vector as needed) was complexed with PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 μg:μl ratio of plasmid to transfection reagent based on manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2021Quote: ... and transfected with RILPL1-miniSOG-HA using LipoD293™ Transfection Reagent (SignaGen Laboratories, SL100668) as described above ...
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bioRxiv - Neuroscience 2021Quote: ... and transfected at 80 % confluence with 1 μg of LRRK2 constructs (and 100 ng of HA-tagged vps35 constructs where indicated) and 3 μl of LipoD293™ Transfection Reagent (SignaGen Laboratories) per well of a 12-well plate overnight ...
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bioRxiv - Microbiology 2021Quote: ... and 1 ng pCMV-IFNL2-genomic or pCMV-IFNL2-cDNA using PolyJet transfection reagent (SignaGen labs) according to the manufacturer’s DNA transfection protocol ...
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bioRxiv - Immunology 2021Quote: ... Transient transfection with plasmids was performed using GenJet version II (SignaGen Laboratories), and transfection with poly(I:C ...
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bioRxiv - Genomics 2021Quote: ... using a PolyJet™ DNA transfection reagent (Signagen Laboratories, 504788). Transfected cells were kept in the culture to allow virus to be released ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated in a 1:1 mixture of DNA/Polyjet DNA transfection reagent for 5-18 hours according to the manufacturer’s protocol (SignaGen Laboratories). Expression of plasmid DNA was selected using G418 ...
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bioRxiv - Microbiology 2020Quote: ... All the cell lines incubated at 37 °C in a humidified atmosphere containing 5% CO2. DNA transfection was performed with GeneJet In Vitro DNA Transfection Reagent (Ver. II) (SignaGen Laboratories) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... then transfected with 1ug of Tau mutant plasmids using PolyJet reagent (SL100688, SignaGen Laboratories, MD). Cells were collected 25 hours after transfection ...
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bioRxiv - Genomics 2020Quote: ... were transformed into 293T cells using PolyJet (SignaGen Laboratories #SL100688) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: 293T cells were cultured in complete DMEM (10% HI-FBS, 1x Pen/Strep) and then transfected using the LipoD293 Transfection reagent (SignaGen Laboratories, cat. SL100668) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: HeLa cells were transfected with a 1:1:1 mix of gRNA1 and gRNA2 vectors together with the donor plasmid using PolyJet transfection reagent (SL100688, SignaGen Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: HEK-293FT cells were transfected approximately 48 hours before imaging using CalFectin (SignaGen Laboratories, catalog number #SL100478). A plasmid ratio of 3:1 for the SunTag:scFv-sfGFP/OsTIR1 plasmids was used ...
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bioRxiv - Bioengineering 2020Quote: ... Transfections were performed using PolyJet DNA In Vitro Transfection Reagent (SignaGen Laboratories) with a PolyJet/DNA ratio of 2.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with plasmid DNA using PolyJet DNA In Vitro Transfection Reagent (Signagen Laboratories, Gaithersburg, USA) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... LV-Syn-DIO-GluN1-2A-tdTomato (Vigene Biosciences, 2 × 109 transducing units (TU)/mL) and LV-Syn-DIO-mTagBFP2-2A-GluN2B (SignaGen Laboratories, 1 × 109 TU/mL). For control fluorophores ...
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bioRxiv - Molecular Biology 2020Quote: All cells were plated 24 h before transfection and transfected at a confluency of 70-80% using GenJet-OS in vitro transfection reagent for U2OS cells (SignaGen Laboratories, Gaithersburg, MD, USA) or JetPRIME for 293T cells (PolyPlus ...
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bioRxiv - Neuroscience 2020Quote: pCAG-eCas9-GFP-U6-Rpsa-gRNA plasmid was transfected into mouse Neuro-2a cells using PolyJet transfection reagent (SignaGen Laboratories), and Genomic DNA was isolated and subjected to PCR to amplify the 433 bp fragment containing gRNA target sequence using Q5 High Fidelity DNA polymerase (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... or PolyJet™ In Vitro DNA Transfection Reagent (SignaGen Laboratories) in DMEM ...
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bioRxiv - Neuroscience 2020Quote: ... Viral particle production and packaging was done by a commercial source (SignaGen Laboratories, Rockville MD, USA). The viral titer is > 1E + 9 TU/ml ...
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bioRxiv - Neuroscience 2020Quote: The lentiviral vector for eGFP under control of the human EF1α-promoter was purchased from SignaGen Laboratories (Gaithersburg MD, USA). All other lentiviral vectors were made in the department of Prof ...
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bioRxiv - Molecular Biology 2020Quote: Plasmid transfection was carried out using LipoD293™ In Vitro DNA Transfection Reagent (SignaGen Laboratories, SL100668) following manufacture's protocol ...
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bioRxiv - Microbiology 2020Quote: ... were transfected with 30 μg full-length proviral plasmid DNA containing the WT sequence or indicated CA mutations using PolyJet DNA transfection reagent as recommended by the manufacturer (SignaGen Laboratories). Two days after transfection ...