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Citations for SignaGen Laboratories :
1 - 50 of 190 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 84 μL of LipoD293TM (SignaGen Laboratories, SL100668) was mixed with 700 μL DMEM medium ...
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bioRxiv - Bioengineering 2024Quote: ... Experiments were performed with rAAV6 vectors produced and purified by SignaGen Laboratories (Frederick, MD, USA) and Packgene Biotech (Houston ...
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bioRxiv - Cell Biology 2024Quote: ... or the PolyJet In Vitro DNA Transfection Reagent (SignaGen Laboratories, SL100688) in DMEM according to the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2024Quote: PolyJet (SignaGen Lab) was incubated with serum-free Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Neuroscience 2024Quote: ... AAV plasmids containing the target genes were co-transfected with pAAV2 (pACG2)-RC triple mutant (Y444, 500, 730F) and the pHelper plasmid (StrateGene) into HEK293T cells by the PolyJet (SignaGen Laboratories, SL100688) transfection reagent ...
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bioRxiv - Molecular Biology 2024Quote: ... or PolyJetTM (SL100688, SignaGen Laboratories), respectively ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV1-TRE-Cre (SignaGen Laboratories, #SL101511) was conducted before jugular surgery ...
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bioRxiv - Neuroscience 2024Quote: ... The lentiviral vectors were assembled using a 3rd generation lentiviral system and CalFectinTM Mammalian DNA Transfection Reagent (SignaGen Laboratories, Rockville, MD) in 293T cells according to the manufacturer protocol ...
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bioRxiv - Microbiology 2024Quote: ... and LipoD293™ In Vitro DNA Transfection Reagent from SignaGen Laboratories (Frederick, MD). Following antibodies were used in this report ...
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bioRxiv - Microbiology 2024Quote: Cell extracts for oligo pulldown assays were isolated from HEK293T cells transfected with an ORF57-expressing vector (pVM7) using LipoD293 transfection reagent (SignaGen Laboratories). Twenty-four hours after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... plasmid expressing sfGFP-TGA-Strep-6xHis-Strep was co-transfected with a plasmid expressing 4 copies of the original or optimized (Opt) ACE-tRNA expression cassettes into HEK293T cells at 75% confluency using Calfectin (SignaGen Laboratories) according to standard protocols ...
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bioRxiv - Cancer Biology 2024Quote: Lentivirus was produced according to the “General Protocol for Generation of lentivirus in 293T cells” using “PolyJet™ In Vitro DNA Transfection Reagent” (Signagen Laboratories). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... using 0.3 μL LipoD293T (SL100668; SignaGen Laboratories). DNA levels were balanced among transfections by addition of the empty pCMV5 vector to 100 ng total DNA ...
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bioRxiv - Biochemistry 2024Quote: ... Viruses were generated by co-transfecting 107 cells plated the previous day in 15 cm dishes with 30 µg of total plasmid DNA (pNLX.Luc.R-.ΔAvrII and a vesicular stomatitis virus G envelope expressor at 6:1 ratio using PolyJet™ DNA transfection reagent; SignaGen Laboratories). Two days post-transfection ...
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bioRxiv - Biochemistry 2024Quote: ... All DNA mixtures were complexed with PolyJet In Vitro DNA Transfection Reagent (SignaGen Laboratories) at a 1:3 μg:μl ratio (plasmid:transfection reagent ...
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bioRxiv - Biochemistry 2024Quote: ... ADGRL3 + GFP and TEN2 + dsRed and 5 μL LipoD293T (SL100668; SignaGen Laboratories). Two days after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... using CalFectinTM Cell Transfection Reagent (SL100478, SignaGen Laboratories; Frederick, MD; USA) according to the manufacturer’s guidelines ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cells were grown to 60% confluency then transfected with 3 μg of each relevant DNA construct using the PolyJetTM transfection reagent (SignaGen Laboratories, USA). 6 hours later ...
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bioRxiv - Microbiology 2024Quote: ... 5 ×105 cells seeded in a 6-well plate were transfected with 40 nM SMARTpool human siRNAs (Horizon) or non-targeting (siNT) control siRNA (Horizon) using LipoJet In Vitro Transfection Kit (Ver. II) (SignaGen Laboratories). Twenty-four hours after the first transfection ...
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bioRxiv - Neuroscience 2023Quote: ... wild type or phosphomimetic tau plasmids using PolyJet (SL100688, SignaGen Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... into 70% confluent HEK293TN cells using PolyJet (SignaGen Laboratories). Media was changed into DMEM containing 1% FetalClone-II serum (Hyclone ...
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bioRxiv - Neuroscience 2023Quote: ... HEK cells were transfected with 2-3 μg of the designated construct or empty vector using the PolyJet (SignaGen Laboratories, SL100688).
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bioRxiv - Molecular Biology 2023Quote: ... DNA transfections for HEK cells used PolyJet (SignaGen Laboratories), whereas MEF cells used TransIT-X (Mirus ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were co-transfected using GenJet™ (cat# SL00488, SignaGen Laboratories) lipofection with Tet-pLKO-puro (Addgene plasmid #21915 ...
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bioRxiv - Neuroscience 2023Quote: We used a GFP-expressing lentiviral vector (LV-EF1α-puro, SignaGen Laboratories, SL100269) to label neuronal precursors and image GC dendritic spines ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected by LipoD293 (SignaGen laboratories #SL100668) at DIV1 ...
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bioRxiv - Cell Biology 2023Quote: ... following the standard LipoD293 transfection protocol (SignaGen Laboratories), with the envelope (pCMV-VSV-G ...
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bioRxiv - Genomics 2023Quote: ... packaging plasmids were cotransfected into 8 million HEK293T cells in a 10-cm dish supplemented with 36 μl PolyJet (SignaGen Laboratories, SL100688). The medium was replaced 12 hours after transfection and harvested every 24 hours thereafter for a total of three harvests ...
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bioRxiv - Genomics 2023Quote: ... we transfected two million MCF7-dCas9/RT cells with 2000 ng of ngRNA/pegRNA plasmid containing an EGFP marker using PolyJet (SignaGen Laboratories, SL100688). Five days after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... packaging plasmids psPAX2 and enveloped plasmids pMD2.G (a gift from Dr. Maciej Olszewski) into HEK-293T cells using lipoD293 (SignaGen Laboratories) as transforming reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... and envelop plasmids pMD2.G (a gift from Dr. Maciej Olszewski) into HEK-293T cells using lipoD293 (SignaGen Laboratories) as transforming reagent ...
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bioRxiv - Bioengineering 2023Quote: ... CD123-E51K and CD123-E51T donor rAAV6 virus was purchased from SignaGen Laboratories (Frederick, MD, USA). CCR5-KO donor rAAV6 virus was produced in HEK-293T cells and was purified with AAVpro Purification Kit (TakaRa ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2-GFP was purchased from SignaGen Laboratories. AAV2-EF1a-Ascl1-Flag was packaged by Viagene ...
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bioRxiv - Cell Biology 2023Quote: ... pMD2.G and psPAX2 in 2:1:2 ratio with PolyJet transfection reagent (SignaGen Laboratories). Media were harvested two days later and added to recipient cells with 1 μg/ml polybrene (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were transfected using GenJet™ In Vitro DNA Transfection Reagent (SignaGen Laboratories, catalog # SL100489-OS) for U2OS Cells as instructed ...
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HIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus MaturationbioRxiv - Microbiology 2023Quote: ... II (SignaGen Laboratories, MD) at a mass ratio of 10:1 ...
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HIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus MaturationbioRxiv - Microbiology 2023Quote: ... II (SignaGen Laboratories, MD) at a 3:1 mass ratio to produce WT and mutant infectious viral particles ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using LipoD293™ Transfection Reagent (SignaGen Laboratories.) Conditioned media containing virions was harvested after 24 and 48 hours ...
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bioRxiv - Genetics 2023Quote: ... The cells were transfected with 5 to 30 nM ZC3HC1 siRNA or 5 to 30 nM control siRNA overnight using GenMute™ SMC siRNA Transfection Reagent (SignaGen Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... using a 1:3 mixture of LipoD293 (SignaGen Laboratories SL100668). 48 hours later ...
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bioRxiv - Molecular Biology 2023Quote: ... and adenoviral Bad* (Ad-Bad*) were produced by SignaGen Laboratories (Maryland, USA). All adenoviruses are based on recombinant serotype 5 (Cat# SL100702 ...
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bioRxiv - Neuroscience 2023Quote: HEK293T cells were transfected with indicated plasmids with GenJet Transfection Reagent (SignaGen Laboratories), and at 48 hr cells were lysed in ice-cold RIPA buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Transfection of HEK293T or HCT116 cells was carried out using LipoD293 (SignaGen Laboratories, Rockville, MD) or FuGENE HD (Promega ...
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bioRxiv - Cell Biology 2023Quote: Cells were seeded in 6-well plates on 30 mm glass coverslips (Paul Marienfeld GmbH & Co. KG, Lauda-Königshofen, Germany) and transfected using PolyJet (SignaGen Laboratories). Per well ...
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bioRxiv - Molecular Biology 2023Quote: The oligonucleotides were delivered using the GenMute™ siRNA transfection reagent for primary macrophages (SL100568-PMG, SignaGen Laboratories), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Plasmids were transfected into 40-50% confluent cells using PloyJet reagent (SignaGen Lab MD, USA). siRNAs were transfected with the siRNA transfection reagent INTERFERin® (Polyplus ...
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bioRxiv - Cancer Biology 2023Quote: ... these compounds were added with the media change prior to the transfection with the PolyJet transfection reagent (SignaGen Laboratories) approximately 12 hours prior to the experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... either using PolyJET DNA transfection reagent (SignaGen Laboratories, Maryland, USA) for plasmid DNA transfection or Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... following manufacturer’s protocols of PolyJet reagent (SignaGen Laboratories).
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bioRxiv - Cell Biology 2023Quote: ... 0.3% PolyJet (SignaGen Laboratories; Frederick, MD), and 1 µg/ml plasmid ...