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Citations for abcam :
501 - 550 of 2129 citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... plates were washed 3 times in 0.1% Tween-20 in TBS and incubated overnight at 4C with antibodies to: MHC II (Abcam ab55152), TZAP (Abnova H0003-B01P) ...
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bioRxiv - Bioengineering 2022Quote: ... HUVECs were washed three times with 1X PBS and then blocked at room temperature for 1 h with a 2.5% goat serum (Abcam, ab7481) solution ...
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bioRxiv - Biophysics 2022Quote: ... in blocking/permeabilization buffer for 120 minutes, washed three times with PBS and visualized with polyclonal anti-rabbit Alexa 488 (IgG, goat, 1:50) (ab11034, Abcam), polyclonal anti-mouse Alexa 488 (IgG ...
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bioRxiv - Microbiology 2022Quote: ... tween 20 in PBS (PBST) three times followed by incubation with goat F(ab’)2 anti-human IgG (Fab’)2-horseradish peroxidase (Abcam) at a 1:10,000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in PBST three times and then mounted onto MAS-GP microscope slides (Matsunami) and coverslipped with Fluoroshield Mounting Media (Abcam). Images were collected on a confocal microscope (Zeiss LSM 710 ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed 5 times with 1× PBST and incubated with goat anti-mouse horseradish peroxidase-conjugated secondary antibody (Abcam) for 30 mins then visualized using the chemiluminescence kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were again washed three times with TBS-T and incubated with an anti-GST HRP-conjugated antibody (Abcam) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were washed three times with PBST and incubated with primary antibodies diluted in blocking solution: 1:500 Rabbit anti-Iba1 (ab178846, Abcam) and 1:250 chicken anti-GFP (A10262 ...
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bioRxiv - Neuroscience 2023Quote: ... washed three times with PBS for 5 min each and incubated with secondary antibody Alexa Fluor® 647 (Abcam, ab150067) for an hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were further rinsed with PBS for 3 times 10 min each and mounted with mounting medium with DAPI (Abcam).
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bioRxiv - Molecular Biology 2023Quote: ... washed 3 times with PBS and incubated with anti-mouse secondary antibodies conjugated with Alexa647 (ab150107, Abcam, 1:1000 dilution) or Alexa488 (A21202 ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed three times with PBS and then labeled with a secondary antibody (1:1000, Alexa Fluor 488, ab150077, Abcam) for 1 h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Isolated m6A-enriched RNA was immunoprecipitated a second time using 2 µg of a second anti-m6A antibody (Abcam ab151230). Final cDNA Libraries were prepared from twice immunoprecipitated RNA and fragmented input total RNA using the Smarter® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio 634411 ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: Tissue sections were washed three times with PBS and incubated in blocking solution [PBS with 10% (v/v) donkey serum (Abcam) and 1% Triton X-100] for 2 hr at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed three times in PBS and incubated with secondary antibodies (Alexa Fluor 488/555, Abcam, 1:500) and DAPI for 2 hours at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Wells were again washed three times for 5 minutes with dPBS before incubating with secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 [Abcam] ...
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bioRxiv - Cancer Biology 2023Quote: ... Magnetics beads (FischerScientific, 13484209) were coated during the same time with 3 µg of the following antibodies: TRIM28 (Abcam, ab10484), YARS (SantaCruz ...
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bioRxiv - Bioengineering 2023Quote: ... sections were washed three times in PBS for 15 min and incubated in blocking solution [PBS with 10% (v/v) donkey serum (Abcam) and 0.5% Triton X-100] for 2 hr at RT ...
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bioRxiv - Biochemistry 2024Quote: ... The cells collected at different time points (0h, 6h, 24h) were analyzed by immunostaining for the presence of γH2A.X (Abcam #ab2893) foci (Syed et al. ...
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bioRxiv - Genetics 2023Quote: ... Cells were washed three times in PBS before the addition of 4′,6-diamidino-2-phenylindole (DAPI) (Abcam, Cambridge, UK) diluted 1:10,000 in H2O ...
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bioRxiv - Cell Biology 2023Quote: ... Organoids were washed with PBS three times again and then cut from inserts and transferred onto glass slides with mounting medium containing DAPI (Abcam) and a coverslip ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 times in PBS then incubated with Alexa 488-conjugated goat anti-rabbit secondary antibody (1:500; ab150077, Abcam) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... The brain sections were washed 3 times with PBS solution before being immersed with chicken anti-GFP antibody (1:500; ab13970, Abcam) for 12 hours at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was washed in TBS-T three times for 5 minutes each and incubated with goat anti-mouse IgG HRP (1:5000, cat# AB205719, Abcam) in TBS-T for 1 h at room temperature ...
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bioRxiv - Genetics 2024Quote: ... slides were washed 3 times with PBS and incubated with isotype-specific anti-rabbit secondary antibody labeled with Alexa Fluor 488 (1:500, Abcam) and anti-mouse secondary antibody labeled with Alexa Fluor 568 (1:500 ...
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bioRxiv - Immunology 2024Quote: ... and were rinsed with PBS for three times before mounting the coverslips to slides in glycerol mounting medium with 4’,6-diamidino-2-phenylindole (DAPI) (ab188804, Abcam). The mounted samples were observed on a Zeiss LSM 880 inverted laser scanning confocal microscope ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed three times with TBST and incubated with HRP-conjugated goat anti-human IgG (Fab’)2 (Abcam) or HRP-conjugated anti-rabbit IgG (Cell Signaling ...
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bioRxiv - Cell Biology 2023Quote: ... Novus), TRACP-5b ELISA kit (EK7661, SAB), OCN ELISA kit (NBP2-68151, Novus) and PDGF-BB ELISA kit (ab224879, Abcam) were used respectively ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we used a calcium assay kit (Abcam Calcium Assay Kit ab102505, USA). After removing the tissue from PBS ...
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bioRxiv - Physiology 2020Quote: ... a nuclear extraction kit (Nuclear Extraction Kit #ab113473, Abcam, Whitby, Ontario, Canada) was used to extract and isolate the nuclear protein fractions of a 5-10 mg sample of BAT ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were washed three times with PBS-T and then incubated with secondary HRP-conjugated anti-rabbit IgG (Abcam, Cambridge, UK) or anti-mouse IgG (Invitrogen™ ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed 3 times with PB and then incubated with the secondary antibody for 2 hours (goat anti chicken Alexa Fluor 594, abcam, ab150172). After incubation with the secondary antibody ...
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bioRxiv - Microbiology 2020Quote: Post-excysted bradyzoites and Day 3- and Day 5-recrudesing populations were inoculated onto 6 well coverslips of HFF or astrocyte cells and at various times were fixed and stained using an anti-toxoplasma antibody (abcam, ab138698). Vacuole sizes at each timepoint were determined by counting 50 random vacuoles in triplicate on a Zeiss Axiovert fluorescent microscope ...
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bioRxiv - Cancer Biology 2020Quote: ... Then slides was washed with washing buffer (0.025% Tween-20/1X PBS) three times and the secondary antibody conjugated with Alexa 555 (ab150086, abcam, 1:1000) was applied ...
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed five times with PBS-T and 100 μL of TMB ELISA Substrate Solution (Abcam, Cambridge, MA, USA) was added to all wells of the plate and incubated for 5 minutes before the reaction was stopped ...
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bioRxiv - Developmental Biology 2019Quote: ... After washing 3 times with TBS-T the blots were incubated with HRP-conjugated secondary antibodies (goat-anti-mouse HRP (Abcam, ab6789); goat-anti-rabbit HRP (Abcam ...
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bioRxiv - Cancer Biology 2020Quote: ... We used extraction buffer and washed several times with PBS before immunofluorescence for ECM using anti-fibronectin-FITC (1:50 dilution; Abcam, ab72686). Samples were imaged using a Zeiss LSM 780 microscope using either a 20x 0.75NA objective or 10x 0.45NA objective.
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed three times for 10 mins with TBST and incubated with IRDye conjugated antibodies (Goat anti-Rabbit IRDye800CW 1:5000 (abcam, #216773), donkey anti-mouse IRDye680RD 1:5000 (abcam ...
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bioRxiv - Genomics 2021Quote: ... Fragmented DNA was then diluted 10 times with ChIP dilution buffer and protein-DNA complexes were captured using an H3K27ac antibody (Abcam AB4729). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were washed with 0.3% PBSTx four times for 15 min per wash and incubated in Alexa Fluor 488 Goat Anti-Mouse IgG H&L (Abcam, ab150117) at a 1:1000 dilution at room temperature (RT ...
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bioRxiv - Developmental Biology 2022Quote: Post-excysted bradyzoites and recrudesing populations were inoculated onto 6 well coverslips of HFF or primary astrocyte cells and at various times were fixed and stained using an anti-toxoplasma antibody (abcam, ab138698). Vacuole sizes at each timepoint were determined by counting 50 random vacuoles in triplicate on a Zeiss Axiovert fluorescent microscope.
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bioRxiv - Cell Biology 2022Quote: ... log-phase liquid cultures were divided in two and incubated for different times in YES-Glucose medium treated with 10 µM 3-BrB-PP1 (Abcam, ab143756), or in medium lacking the analog kinase inhibitor ...
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bioRxiv - Neuroscience 2022Quote: ... Free floating sections were washed three times in PBS (10 minutes each) and then incubated in a blocking solution containing 10% normal goat serum (NGS, Abcam #ab7481), 0.5% Triton X-100 in PBS for 2 hours at room temperature on a shaker ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed at least three times with PBST and incubated with native Streptavidin protein DyLight 488 (1:750 dilution; Abcam, ab134349) in 0.5% fish skin gelatin with PBST for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then washed 3 times in 1x PBS for 10 minutes then incubated in DAPI counterstain (Abcam, ab228549; 2 µM) for 30 minutes before being washed 3 more times in 1x PBS for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... Slides were washed three times with PBS before staining with Donkey anti-goat-AlexaFluor 594 secondary antibody (1:200, abcam #ab150132). Stained slides were washed again ...
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bioRxiv - Microbiology 2023Quote: ... washed further 5 times with PBS pH 7.4 and incubated for one hour with Goat anti-monkey (Rhesus macaque) (Abcam cat # a112767), diluted 1:2000 in PBS pH 7.4 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 3 times with PBS for 5 minutes and incubated with AlexaFluor® 647 conjugated secondary antibodies (Ab150079, Ab150115; Abcam) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The plates were washed three times with PBS-T and 100 uL anti-hamster IgG horseradish peroxidase antibody (HRP, abcam, #ab6892) diluted 1:10,000 in PBS-T containing 1% milk powder was added to all wells ...
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bioRxiv - Plant Biology 2022Quote: ... pH 7.5) three times, incubated in blocking solution (1% [w/v] bovine serum albumin, 1% [w/v] normal goat serum (Abcam ab7481) in TBS with 0.2% [v/v] Tween-20 [TBST]) ...