Labshake search
Citations for abcam :
451 - 500 of 2129 citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... the cells were further washed with PBS and then washed two times with wash buffer (10% formamide (Abcam) in 2x saline sodium citrate (SSC) ...
-
bioRxiv - Immunology 2021Quote: ... Plates were washed 3 times with PBST and then incubated with a goat anti-human HRP (abcam ab7153) secondary ...
-
bioRxiv - Immunology 2020Quote: ... and adipose tissue was collected from surviving mice at 48hr time point for western blot of UCP1 (abcam) expression.
-
bioRxiv - Developmental Biology 2023Quote: ... Samples were then washed 6 times in PBSGT before incubation with secondary antibodies (ab150063 and ab175745 from Abcam, at 1:500 and 1:250 dilution ...
-
bioRxiv - Immunology 2023Quote: ... Sections were then washed five times with PB buffer and blocked in 10 % normal goat serum (NGS; Abcam) at 4°C for 1.5 h ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by three times PBS washing and then incubation with goat anti-cTnnI3 (1:500 Abcam Cat# ab56357) for an additional overnight at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... three pre-diluted primary antibodies were sequentially applied in the following order using the indicated chromogenic detection: anti-CASQ1 (1:10000, Abcam # ab185220, Abcam, Cambridge, UK) with Omap anti-Ms HRP (Ventana Discovery #760-4310 ...
-
bioRxiv - Cell Biology 2023Quote: ... DNA detection was performed by applying 2 ng of the sample collected for Pico Green detection to a nylon membrane and detecting with the α-dsDNA antibody (abcam ab27156, 1:7000).
-
bioRxiv - Immunology 2023Quote: ... washed four times with PBS-T and 20 μL of polyclonal HRP-conjugated detection antibodies (goat anti-mouse, #G-21040, Thermo Fisher at 1:1,000 dilution or goat anti-human, #ab7153, Abcam at 1:10,000 dilution) were added to each well and incubated for 30 min (anti-mouse ...
-
bioRxiv - Molecular Biology 2023Quote: ... 300 ng/mL RIPK1 primary detection antibody (1:300 dilution in Meso Scale Discovery Diluent 100 of Abcam ab125072 lot GR3258748, 50 µL/well) was incubated for 2 h at RT with shaking at 600 rpm ...
-
bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475, Lot GR3234297-32) for 20 minutes at room temperature before being incubated with PBS containing both WGA-555 (Thermo Fisher ...
-
bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475, Lot GR3234297-32) for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher ...
-
bioRxiv - Physiology 2022Quote: ... 0,05% Tween 20 (TBS-t) for 60 min at RT and incubated overnight at 4°C with anti-IL6 antibody (Abcam, ab229381, 1:1000 dilution). Following 3 washes (10 min ...
-
bioRxiv - Immunology 2020Quote: ... Genotyping PCR products were digested with AvaI restriction enzyme (Abcam, Cambridge, Massachusetts) for 1 hour at 37°C and electrophoresed on a 3% agarose gel in 1X Tris-acetate-EDTA (TAE ...
-
bioRxiv - Plant Biology 2023Quote: ... and then was first incubated with rabbit anti-AKIN10 antibody (1:500 dilution) followed by incubation with Veriblot IP detection reagent (Abcam, 1:500 in blocking solution). Protein bands were detected with SupersignalTM west pico chemiluminescent substrate (Thermofisher ...
-
bioRxiv - Biochemistry 2023Quote: ... Fluorescently labelled detection antibodies (AT8: Invitrogen, Cat. No. MN1020, 5 nM; HT7: Invitrogen, Cat. No. MN1000, 2 nM; T181: abcam, Cat. No. ab232849, 5 nM) were added in blocking solution ...
-
bioRxiv - Biochemistry 2023Quote: ... Nucleosomes bound were detected using western blot followed by detection with histone antibodies (anti-H3 Abcam ab1791; anti-H2A Abcam ab18255, anti-H2B Abcam ab1790) and HRP conjugated secondary antibodies (anti-mouse IgG-HRP Vector Labs PI2000 ...
-
bioRxiv - Microbiology 2021Quote: ... cells were washed two times in PBS and stained with goat anti-mouse secondary IgG-HRP antibody (abcam, 6823) at a dilution of 1:5000 in 1% milk-TBST and incubated for 1 hour at RT ...
-
bioRxiv - Cancer Biology 2020Quote: ... Bead pellet was washed three times with 0.5% BSA in PBS before incubating in 1:200 rabbit anti-human NM23A (Abcam), NME2 (Abcam) ...
-
bioRxiv - Cell Biology 2022Quote: ... the membrane was washed three times with TBS-T and incubated with HRP-anti-Mouse secondary Ab (Abcam #ab205719)(1:1000 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Slides were washed three times with PBS for five minutes and incubated with H3K4me3 antibody (Abcam ab8580, 1:10’000), H3K27me2me3 antibody (Active Motif 39535 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... the membranes were washed three times with 5% nonfat dry milk in 0.1% TBST for 30 min each time and incubated with horseradish peroxidase-conjugated secondary antibody (Abcam) at room temperature for 1 h ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The membrane was then washed three times in PBST and probed with a secondary horseradish peroxidase-conjugated antibody (Abcam) at a dilution of 1:15,000 in 1x PBST ...
-
bioRxiv - Immunology 2019Quote: ... washed 3 times with PBS/1% BSA/0.05% Tween (TBPBS) and stained with goat-anti-Iba1 1/200 (ab5076, Abcam) and anti-phosphotyrosine (pTyr ...
-
bioRxiv - Microbiology 2020Quote: ... washed 3 times with TBS-T and primary 4-HNE antibody was added at 1:200 dilution (Abcam, #ab46545). The antibody was left on overnight at 4°C rocking ...
-
bioRxiv - Microbiology 2020Quote: ... washed three times with TBS-T and primary 4-HNE antibody was added at 1:200 dilution (Abcam, #ab46545). The antibody was left on overnight at 4°C rocking ...
-
bioRxiv - Cancer Biology 2023Quote: ... fragmented and run-on reaction products were immuno-purified two times using anti-Br-UTP antibody (ab-6326, Abcam) bound to 30 μl of magnetic beads (Protein G DynabeadsThermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... Plates were then washed six times in PBST and added with 100µl of goat anti-rabbit IgG (Abcam, #ab6721) at a 1:8000 dilution for 1 hour ...
-
bioRxiv - Biophysics 2023Quote: ... Membranes were washed three times with 1X TBST before incubating with horseradish peroxidase-conjugated goat antib-rabbit secondary antibody (1:10,000; Abcam) diluted in blocking buffer for 1 h at 4 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... Three times washing with PBS followed by secondary antibodies goat anti-rabbit IgG Alexa Fluor 555 (Abcam Cat# ab150078), donkey anti-goat IgG Alexa Fluor 488 (ThermoFisher Cat# A-11055) ...
-
bioRxiv - Cancer Biology 2024Quote: ... the membranes were washed three times with 5% nonfat dry milk in 0.1% TBST for 30 min each time and incubated with horseradish peroxidase-conjugated secondary antibody (Abcam) at room temperature for 1 h ...
-
bioRxiv - Pathology 2023Quote: ... Sections were washed 3 times for 5-10 min in 0.3% PBT and incubated with secondary antibodies and Hoechst (Abcam) in 3% NDS in 0.3% PBT for 1 h at RT ...
-
bioRxiv - Biochemistry 2022Quote: ... and re-probed (1h, RT, 1/10000 diluted in PBS-Tween with 5% non-fat milk) with rat anti-β actin (AbCam, Cambridge, UK, RRID: AB_867488) washed (as above ...
-
bioRxiv - Microbiology 2022Quote: ... Phosphate kit: Phosphate Assay Kit (Colorimetric) (Abcam, product code: ab65622). Sodium kit ...
-
bioRxiv - Microbiology 2022Quote: ... Potassium kit: Potassium Assay Kit (Fluorometric) (Abcam, product code: ab252904). Osmolarity was measured in each SM sample using an osmometer (Osmomat 030 by Gonotec).
-
bioRxiv - Bioengineering 2020Quote: ... c-Kit (Abcam), and mast cell tryptase (Abcam) ...
-
bioRxiv - Neuroscience 2023Quote: ... 1% bovine serum albumin in PBS with 0.1% Tween20 [PBST]) for one hour at RT and left overnight in the A2AR primary antibody at 4°C (Abcam #ab3461, Lot # GR3431019-2, 1:100) in blocking solution ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.05% Triton X-100 in PBS) was performed for 1 hour at RT followed by primary Ab (ab237715, Abcam, diluted 1:1000, 0.1% FBS in PBS) incubation for 1 hour at RT ...
-
bioRxiv - Bioengineering 2019Quote: ... Pro-inflammatory prostaglandin E2 (PGE2) was also analyzed from media samples (n=3/condition/cell-type/time-point) via enzyme-linked immunosorbent assay (Abcam) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were washed five times with PBS before incubation with secondary antibodies at room temperature for 2 h (Alexa 488, 1:1000; abcam). Finally ...
-
bioRxiv - Cancer Biology 2022Quote: ... felis for 4-4.5 mos and then were injected intraperitoneally three times with 1.2 mg/kg of a monoclonal anti-IFNα antibody (Abcam, #ab7373) spaced three days apart for each administration for a total of 72 μg ...
-
bioRxiv - Bioengineering 2022Quote: ... plates were washed 10 times and then developed by adding 50 μL of TMB ELISA Substrate (High Sensitivity) (Abcam, ab171523). Development was stopped after 2.5 min by adding 1 N hydrochloric acid ...
-
bioRxiv - Bioengineering 2022Quote: ... sections were washed with PBS three times for 15 min and incubated in blocking solution [PBS with 10% (v/v) donkey serum (Abcam) and 0.5% Triton X-100] for 2 hours at RT ...
-
bioRxiv - Genomics 2019Quote: ... Membranes were then washed with TBST three times and incubated with Goat Anti-Rabbit IgG H&L (HRP) secondary antibody 1:10,000 dilution (Abcam ab205718) for 1 hour at room temperature ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Wells were washed four times and then filled with 100 μL of Goat anti-GFP conjugated to Horseradish Peroxidase (ab6663, Abcam) diluted 1:3000 in Receptor Assay Blocking buffer ...
-
bioRxiv - Immunology 2021Quote: ... plates were rinsed three times with PBST and incubated with peroxidase-conjugated goat anti-mouse IgA or goat anti-rat IgA (Abcam), for 1 hr ...
-
bioRxiv - Biochemistry 2020Quote: ... Livers and blood samples were collected at this time point for H&E staining and measurement of AST/ALT (Abcam), GSH/GSSG (DojinDo) ...
-
bioRxiv - Physiology 2021Quote: ... Samples were then washed three times with PBT 0.1% and incubated with a secondary goat anti-mouse FITC antibody (Abcam #6785) for 1h30 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Unbound protein was removed by washing the wells three times with 100ul of washing buffer and bound Myc-HK2 was detected with monoclonal anti-Myc HRP-conjugated antibody (Abcam; 1:5000 in blocking buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... The wells were then washed three times with PBS buffer (0.05% tween) and treated with HRP-conjugated anti-FITC antibody (100 μL/well, Abcam, ab196968) for 1h at room temperature ...