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Citations for abcam :
401 - 435 of 435 citations for SPINK6 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... neurons were incubated first with a blocking solution (BSA 1%, NGS 2%, Triton X-100 0.1%) and then with primary antibodies for KIF1A (Abcam, #ab180153, 1:100, rabbit), HTT (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... blocked with blocking solution at 37°C for 3 h and incubated for 2 h with primary antibody (H3K9ac – Abcam ab12179, 1:300) and 1 hour with secondary antibodies (Donkey anti mouse – Abcam ab150109 ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were subsequently incubated in Inoue blocking solution (Inoue and Wittbrodt, 2011) (5 % Normal Goat Serum (NGS) (Abcam, cat# ab7481, lot# GR325285-5), 2% BSA (Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... slices were pre-incubated at room temperature for 2 hours in a blocking medium composed of PB-Tx and Normal Goat Serum (NGS, 5% or 10%, Abcam, Cat. No. ab7481), after which they were incubated with primary antibodies (72 hours at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Blocking buffer was discarded and slides were washed and incubated with primary antibody: rabbit anti-Alpha-synuclein (phospho S129) (Abcam #ab51253, 1:10000) diluted in blocking buffer overnight ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed 3 times in 1x PBS for 10 minutes then incubated in blocking solution containing AlexaFlour488 conjugated secondary antibodies (Abcam, ab150077; 1:1000) for 2-4 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were washed three times in PBST and incubated with secondary antibodies diluted in blocking solution for two hours at room temperature (RT): Goat anti-Rabbit conjugated Alexa Fluor 568 (Abcam #175471, 1:500) and 1:500 Goat anti-Chicken Alexa Fluor 488 (A11039 ...
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bioRxiv - Physiology 2023Quote: ... Membranes were blocked with Odyssey blocking buffer (Li-COR) and immunoblotted overnight (4°C) with rodent OXPHOS cocktail monoclonal antibody (Abcam; ab110413, 1:1000) to detect ETC proteins ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated at 4°C for 72 hours in blocking solution (5% bovine serum albumin, 0.2% Triton-X) containing primary antibodies CD45 antibody (Abcam, ab10558, 1:400 dilution), CD11b (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were washed with PBS and incubated in the blocking solution containing goat anti-mouse IgM Alexa Fluor 488 (Abcam, ab150121, 1:500) antibody and goat anti-mouse IgG H&L Alexa Fluor 594 (Abcam ...
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bioRxiv - Genomics 2023Quote: ... cells were immunostained in blocking buffer containing mouse anti-beta III Tubulin (Tub) conjugated Alexa Fluor 488 antibody (Abcam ab195879, dilution 1:1000) and DAPI (Sigma D9542 ...
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bioRxiv - Bioengineering 2023Quote: ... After completion of blocking the membrane was incubated in primary antibody overnight on a shaker (Primary antibodies: Syntenin-1 (Abcam, ab133267 1:2000); CD81 (Santa Cruz ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were incubated in blocking solution for 2 hr and then incubated with goat anti-rabbit-HRP secondary antibody (Abcam; ab6721; 1:300) overnight at 4°C ...
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bioRxiv - Physiology 2021Quote: ... Pre-synaptic motoneuron terminals were then labeled by incubating bundles overnight at 4°C in the same blocking solution to which had been added a mouse anti-synaptophysin antibody (AB8049 Abcam, USA; 1:25 dilution). The following morning bundles were washed (5 x 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... After blocking in 5% milk/TBST membranes were probed with primary anti-ACE2 antibody (Abcam 15348 for total ACE2 or Abcam 108252. for long ACE2) followed by the appropriate secondary HRP-conjugated antibody (Dako) ...
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bioRxiv - Immunology 2021Quote: ... detected with HRP conjugated secondaries in blocking buffer (Goat anti-Rabbit HRP, Sino Biological SSA003, 0.5 μg/ml or Goat anti-Mouse HRP, Abcam ab205719, 1: 4000 dilution) and visualised using the SuperSignal West Femto kit (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 h at room temperature and then with primary antibodies diluted in the blocking solution overnight (anti-PV primary antibody, ab11427, Abcam, Cambridge, UK; 1:1000) at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were diluted in blocking buffer and applied to cells overnight at 4°C: chicken anti-βIII tubulin (Tuj-1, 1:200; Abcam #ab41489; Boston, MA, USA), rabbit anti-PSD95 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... The blocking step was followed by incubation with rabbit anti-NeuN-Alexa Fluor 647 or 488 (1:500, Abcam, ab190565 or ab190195, clone EPR12763) in PBS containing 1% BSA and 0.2% Triton X-100 overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The higher molecular weight part of the membrane was incubated overnight at 4ºC in anti-DNAK antibody (1:5000 dilution in blocking buffer, Abcam#69617 Mouse monoclonal [8E2/2]) as the loading control ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then incubated overnight in primary antibody in the blocking solution at 4°C using 1 or more of the following antibodies: α-SMA antibody (ab7817, 1:500, Abcam, Cambridge, MA, USA), FAP antibody (ab53066 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained overnight at 4°C in blocking solution with the following antibodies and dilutions: Alexa Fluor® 488 anti-S100 beta (1:1000, Abcam, #ab196442, clone EP1576Y), PE anti-Nestin (1:150 ...
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bioRxiv - Biochemistry 2020Quote: ... After blocking with 5% defatted milk, membranes were incubated (overnight, 4°C) with primary antibodies (anti-NNT cat. Abcam: ab110352; β-actin cat. Abcam ab8226). Membranes were then incubated with secondary antibodies conjugated to fluorescent (Licor® ...
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bioRxiv - Immunology 2021Quote: ... before incubation with primary antibodies in blocking buffer (Rabbit polyclonal anti-SARS-Cov2, Sino Biological 40591-T62, 1:6000 dilution or Mouse b-actin, Abcam 8226, 1 μg/ml), detected with HRP conjugated secondaries in blocking buffer (Goat anti-Rabbit HRP ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were applied in blocking buffer at 4°C over-night (alpha-tubulin, Abcam ab233661, 1:1000 dilution and Pcdh19, Abcam ab191198, in 1:200 dilution). After 3 wash steps in PBS-T (0.1% triton) ...
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bioRxiv - Immunology 2022Quote: ... table 1) were diluted in blocking solution (F4/80 [Bio-Rad]: 10 µg/ml, Ly6G [abcam]: 1 µg/ml, MHC-II [BioLegend]: 5 µg/ml), CD163 [Invitrogen] ...
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bioRxiv - Plant Biology 2023Quote: ... and then was first incubated with rabbit anti-AKIN10 antibody (1:500 dilution) followed by incubation with Veriblot IP detection reagent (Abcam, 1:500 in blocking solution). Protein bands were detected with SupersignalTM west pico chemiluminescent substrate (Thermofisher ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:500 rabbit anti-PARP1 Abcam ab227244; 1:500 rabbit anti-PCNA Abcam ab18197, 1:500 mouse anti-PCNA Abcam ab29 diluted in blocking solution). After antibody incubation ...
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bioRxiv - Biochemistry 2024Quote: ... The coverslips were washed twice with 1x PBS and incubated with the secondary antibody (donkey anti-mouse coupled to Alexa Fluor 594, Abcam; 1:500 in blocking buffer) for 60 min at room temperature in the dark ...
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bioRxiv - Microbiology 2019Quote: ... Ultra-thin sections were blocked with 5% fetal bovine serum and 5% normal goat serum in 1× PBS for 30 min and subsequently stained with rabbit anti-GFP antibody (Abcam, 1:500 dilution in blocking buffer), washed thoroughly and incubated with 18 nm colloidal gold-conjugated anti-rabbit IgG for 1 h ...
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bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were incubated overnight at 4°C with the following primary antibodies diluted in blocking solution: chicken polyclonal anti-mouse GFAP (Abcam ref. ab4674, RRID AB_304558, 1:3000), rabbit polyclonal anti-mouse IBA1 (Wako ref ...
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bioRxiv - Cell Biology 2021Quote: ... Non-specific interactions were blocked for 1 hour with blocking solution (3% BSA, Sigma ref. A9647, 2% donkey serum, Abcam ref. ab7475, 0,3% Triton X-100, PBS). Primary antibodies were diluted in the blocking solution and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated for 1h in blocking buffer (PBS, 10 % FBS, 1 % Saponin) followed by primary antibody incubation overnight at 4°C (Calnexin, Abcam ab22595 at a dilution of 1:200). After washing twice with wash buffer (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated overnight with the primary antibody solution at 4 °C (blocking buffer solution contained primary antibodies with the following dilution: ch α GFP 1:2000, Abcam; rb α βIV-spectrin 1:1000, self-made 4). Subsequently ...