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Citations for abcam :
251 - 300 of 435 citations for SPINK6 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were blocked using Odyssey® blocking buffer (Licor, 927-40000) and then incubated with antibodies against KLF5 (abcam, ab137676), Tubulin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... blocked with Odyssey Blocking Buffer (Li-Cor 927-40000) and incubated with CENP-A Mouse antibody (1:500; Ab13939; Abcam) in 5% BSA TBS with 0.1% Tween 20 and detected with IRDye 800CW and 680LT secondary antibodies using the Odyssey Infrared Imaging System (Li-Cor).
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bioRxiv - Molecular Biology 2020Quote: ... at room temperature for 2 h and then incubated overnight at 4°C in Blocking Buffer containing primary antibody (anti-mouse PIWIL2/MILI, Abcam Cat# ab36764 ...
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bioRxiv - Immunology 2020Quote: ... thawed unfixed tissue slices were rinsed with PBS then blocked with blocking solution (PBS supplemented with 2% Bovine Serum Albumin (Roth) and 5% Normal Goat Serum (Abcam)) for 1 hour at room temperature before incubation of mAbs at 5 µg/ml overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Blocking was followed by an additional 20 min incubation on ice with antibodies of interest (GSDMD-PE 1:500, Abcam). Mitochondria were washed 2X in mitoFLOW buffer and were analyzed on an LSR Fortessa X20 (BD Biosciences) ...
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bioRxiv - Biochemistry 2022Quote: ... washed three times in blocking buffer and then incubated in 1:500 dilution of Alexa-488-conjugated goat anti-rabbit secondary (ab150077; Abcam) mixed with 1X rhodamine-phalloidin (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... slides were washed 3 × 1 minute in PBS before exposing to a blocking solution containing TBST (TBS with 0.1% Tween-20) plus 20% AquaBlock (Cat. # ab166952, Abcam USA) for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were added to the blocking solution (Rabbit anti-DsRed 1:1000, stock #632496, Takara Bio, Clonetech; chicken anti-GFP 1:1000, ab13970, Abcam) and incubated overnight at 4°C ...
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bioRxiv - Genetics 2022Quote: ... the wells were blocked with Licor blocking buffer (927-40000, Licor, Cambridge, UK) for 90 minutes followed by incubation with Anti-ASL antibody (Abcam) for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... They were then blocked with rotation at room temperature for an hour in blocking buffer (5% normal goat serum (Abcam), 0.2% TritonX-100 PBS) ...
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bioRxiv - Neuroscience 2022Quote: ... Alexa Fluor secondary antibodies were diluted 1:500 in blocking solution and included 568 goat anti-Armenian hamster (Abcam, # ab175716), 555 goat anti-rat (Invitrogen #A21434) ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were washed three times with PBST and incubated with primary antibodies diluted in blocking solution: 1:500 Rabbit anti-Iba1 (ab178846, Abcam) and 1:250 chicken anti-GFP (A10262 ...
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bioRxiv - Neuroscience 2023Quote: ... PFC sections were incubated overnight at 4°C in a blocking solution containing primary antibodies against green fluorescent protein (GFP; 1:1000 dilution, raised in goat, AB6673, Abcam). VTA sections were incubated overnight in a blocking solution containing antibodies for green fluorescent protein (GFP ...
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bioRxiv - Neuroscience 2023Quote: ... VTA sections were incubated overnight in a blocking solution containing antibodies for green fluorescent protein (GFP; 1:1000 dilution, raised in goat, AB6673, Abcam) and tyrosine hydroxylase (TH ...
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bioRxiv - Neuroscience 2023Quote: ... sections were rinsed in blocking buffer (2x 10 min) and incubated with secondary antibodies: Alexa Fluor 488 goat anti-mouse (1:1000, Abcam), Alexa Fluor 594 goat anti-rabbit (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with the secondary antibody (goat anti-chicken with Alexa Fluor 488 1:800 in blocking solution; ab150169, Abcam) for 2 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were incubated with the blots overnight at 4 °C in blocking solution: anti-myc-tag (1:5,000, rabbit pAb, ab9106, Abcam, UK), anti-Dclk1 (1:1,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were washed 3 times with PBS with 0.1% Tween-20 and were incubated for 1 hour in the following secondary antibodies diluted in blocking buffer (Anti-Guinea Pig AF 405 1:750 (Abcam), Anti-Chicken AF 488 (Abcam) ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was washed and incubated first in blocking buffer and then with an anti-m6A antibody (Abcam, ab151230, USA) overnight at 4 °C with gentle shaking ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were rehydrated with PBS for 5 minutes before blocking and permeabilization for 1 hour with 10% normal goat serum (Abcam) containing 0.3% PBS-TritonX-100 ...
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bioRxiv - Neuroscience 2023Quote: ... 2% goat serum) for 1h and then incubated overnight at 4°C in blocking buffer containing anti-PSER129 antibody EP1536Y (Abcam) diluted 1:50,000 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 0.3% Triton X-100 and 90%PBS (Blocking Buffer) for 1 hr at room temperature and then incubated in primary antibodies (rabbit anti-Iba1, Abcam, ab178847 ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then incubated with the following primary antibodies diluted in blocking buffer at room temperature for 2 hours: anti-OCT4 (1:200, catalog no. ab181557; Abcam); anti-TFAP2C (1:100 ...
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bioRxiv - Developmental Biology 2023Quote: 12μm thick cryosections of organoids were permeabilized in 0.1% Triton/PBS before incubation with primary antibodies at 1/200 dilution using appropriate serum as blocking buffer without antigen retrieval step: SOX2 (rabbit, ab92494 Abcam), TBR2 (sheep ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were washed 3 times with PBS with 0.1% Tween-20 and were incubated for 1 hour in the following secondary antibodies diluted in blocking buffer (anti-guinea pig AF 405 1:750 (Abcam), anti-chicken AF 488 (Abcam) ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS and then incubated overnight with gentle agitation at 4 °C in blocking solution plus 1:1000 dilution primary antibody (chicken anti-GFP polyclonal, Abcam; rabbit anti-dsRed polyclonal ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained in blocking buffer overnight at 4°C with the following primary antibodies at the indicated dilutions: anti-Gal-9 (Abcam ab275877 ...
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bioRxiv - Developmental Biology 2023Quote: ... BSA (blocking agent) was applied on slides for 35 minutes followed by overnight incubation with primary antibodies [MMP-2 (Abcam) at a dilution of 1:100 ...
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bioRxiv - Genomics 2023Quote: ... Tissues were stained at 4 °C overnight using antibodies diluted in blocking solution: anti-EpCAM-AF594 (abcam, ab286811; 1:200) and anti-Vimentin-AF488 (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were diluted in blocking reagent and incubated overnight at 4 °C (Collagen I (# ab34710, Abcam, Collagen IV (#ab6586, Abcam), MUC-1 (MA1-06503 ...
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bioRxiv - Cancer Biology 2023Quote: ... The following primary antibody was incubated overnight at 4°C in blocking buffer: LINE-1 ORF1p (ab230966, Abcam, 1:200). Vector ImmPress anti-Rabbit HRP and ImmPact DAB kits (Vector Laboratories ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: Tissue sections were washed three times with PBS and incubated in blocking solution [PBS with 10% (v/v) donkey serum (Abcam) and 1% Triton X-100] for 2 hr at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... for 2 h and then incubated overnight at +4°C with primary antibodies in 1:1 TBS and blocking buffer to analyze the content of pRLC (1:1000; ab2480, Abcam), Total OXPHOS Cocktail (OXPHOS ...
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bioRxiv - Bioengineering 2023Quote: ... sections were washed three times in PBS for 15 min and incubated in blocking solution [PBS with 10% (v/v) donkey serum (Abcam) and 0.5% Triton X-100] for 2 hr at RT ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation at 4◦C overnight with primary antibodies diluted in the blocking buffer (rabbit anti-Dclk1 1:1000, Abcam, ab31704 ...
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bioRxiv - Immunology 2023Quote: ... The membranes then were incubated at 4◦C overnight with the primary antibodies diluted in the blocking buffer (rabbit anti-NLRP3 1:1000, Abcam, ab263899; rabbit anti-Gsdmd 1:1000, Abcam, ab219800 ...
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bioRxiv - Immunology 2023Quote: ... The membranes then were incubated at 4◦C overnight with the primary antibodies diluted in the blocking buffer (rabbit anti-NLRP3 1:1000, Abcam, ab263899 ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation at 4◦C overnight with primary antibodies diluted in the blocking buffer (rabbit anti-Dclk1 1:1000, Abcam, ab31704; rabbit anti-Dclk1-488 1:1000, Abcam, ab202754 ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then washed 3X in 0.4% Triton-X-100 TBS for 5 minutes before blocking at room temperature for 2 hours in a solution of 10% Normal Goat Serum (Abcam), 3% BSA (Fisher ...
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bioRxiv - Immunology 2024Quote: ... Sections were then incubated for 48 hours with the following primary antibodies (1:100 in blocking solution): Rat anti-P21 (ab107099; Abcam), and Rabbit anti-CDKN2A/p16INK4a (EPR20418 ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were then incubated for 60 minutes at room temperature in blocking buffer (PBS with 5 % goat serum [Abcam #ab7481] and 0.3 % Triton X-100 [Bio-Rad Laboratories #161-0407]) ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were blocked with BloxAll blocking buffer and 2.5% horse serum respectively prior to a 1-h incubation with anti-Ki67 antibody (Abcam, ab16667) at 1:100 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated for 72 hours at 4°C with the following dilutions of the primary antibodies in blocking buffer: Pecam1 1:50 (ab28364, Abcam), Keratin 8 1:100 (Troma1 ...
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bioRxiv - Microbiology 2020Quote: ... coli anti-CsgD peptide primary antibody (dilution 1:5000) (79) and anti-Flagellin (FliC) antibody (Abcam, dilution 1:5000), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies C-peptide (DSHB, 1:200) and glucagon (MilliporeSigma, G2654, 1:10,000) diluted in antibody dilution buffer (Abcam) were added to the wells for overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Sections were then washed in TBS and stained in 10% FBS in TBS-T (0.05% Tween-20) blocking solution with primary antibodies: anti-GFP 1:2000 (Abcam Cat# ab13970), anti-RFP 1:1000 (Rockland p/n ...
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bioRxiv - Genetics 2021Quote: ... were incubated in blocking solution (0.5% bovine serum albumin in PBS) with 3 μg of antibody to histone-H3 K27ac (Abcam, cat. # ab4729) for 3 h at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... then incubated 1 hour at room temperature in blocking buffer containing a 1:10,000 dilution of goat anti-Rabbit IgG H+L HRP-conjugated (Abcam Cat #ab63797). Blots were washed again in 3X TBS-T then incubated in blocking buffer containing a 1:5000 dilution of rabbit Anti-DDDDK HRP conjugated antibody (Abcam Cat #ab2493) ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were incubated in primary antibodies diluted in blocking solution overnight at 4°C as follows: rabbit anti-CASP3 (1:500, Abcam, ab49822); mouse anti-COL2A1 (1:400 ...
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bioRxiv - Neuroscience 2021Quote: ... Microglia were probed with a 24h incubation at 4°C with either a rabbit antihuman IBA1 antibody diluted in blocking solution (1:200, 019-19741, Wako chemicals, USA) or a goat anti-human IBA1 antibody (1:200, ab5076, Abcam, UK). We used additional microglial markers including mouse anti-MHC-II/HLA-DP/DQ/DR (1:250 ...